JP4884628B2 - 発現の増強 - Google Patents
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- JP4884628B2 JP4884628B2 JP2001540262A JP2001540262A JP4884628B2 JP 4884628 B2 JP4884628 B2 JP 4884628B2 JP 2001540262 A JP2001540262 A JP 2001540262A JP 2001540262 A JP2001540262 A JP 2001540262A JP 4884628 B2 JP4884628 B2 JP 4884628B2
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| GB0023668A GB0023668D0 (en) | 2000-09-27 | 2000-09-27 | Enhanced expression |
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| PCT/GB2000/004454 WO2001038512A2 (en) | 1999-11-22 | 2000-11-22 | Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) |
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| CA2492917C (en) | 2002-07-19 | 2011-10-18 | University Of South Carolina | Compositions and methods for the modulation of gene expression in plants |
| US7683238B2 (en) | 2002-11-12 | 2010-03-23 | iBio, Inc. and Fraunhofer USA, Inc. | Production of pharmaceutically active proteins in sprouted seedlings |
| US7692063B2 (en) | 2002-11-12 | 2010-04-06 | Ibio, Inc. | Production of foreign nucleic acids and polypeptides in sprout systems |
| EP1594956A4 (en) | 2003-02-03 | 2007-08-01 | Fraunhofer Usa Inc | SYSTEM FOR EXPRESSION OF GENES IN PLANTS |
| AU2004278624B2 (en) * | 2003-10-01 | 2008-03-20 | Japan Science And Technology Agency | DNA fragment, method for producing transformant for protein production and utilization thereof |
| GB0327919D0 (en) * | 2003-12-02 | 2004-01-07 | Plant Bioscience Ltd | Enhanced expression |
| ES2532609T3 (es) * | 2004-02-20 | 2015-03-30 | Ibio, Inc. | Sistemas y métodos para expresión clonales en plantas |
| PL1756280T3 (pl) | 2004-04-22 | 2015-04-30 | Commw Scient Ind Res Org | Synteza długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach rekombinowanych |
| ES2529572T3 (es) | 2004-04-22 | 2015-02-23 | Commonwealth Scientific And Industrial Research Organisation | Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes |
| EP1616959A1 (en) * | 2004-07-07 | 2006-01-18 | Icon Genetics AG | Biological safe transient protein expression in plants |
| FR2877014B1 (fr) * | 2004-10-21 | 2009-07-10 | Inst Rech Pour Le Dev I R D Et | Systemes d'expression de proteines recombinantes et leurs applications |
| FR2880357B1 (fr) * | 2005-01-05 | 2010-12-10 | Inst Rech Developpement Ird | Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications |
| WO2007092460A2 (en) * | 2006-02-03 | 2007-08-16 | The Texas A & M University System | Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants |
| BRPI0716075A2 (pt) | 2006-08-29 | 2013-08-06 | Commw Scient Ind Res Org | sÍntese de Ácidos graxos |
| EP1897953A1 (en) * | 2006-09-06 | 2008-03-12 | Icon Genetics GmbH | Potexvirus-derived replicon |
| US9605270B2 (en) * | 2006-09-22 | 2017-03-28 | The Regents Of The University Of California | Chemically inducible cucumber mosaic virus protein expression system |
| WO2008094512A2 (en) | 2007-01-29 | 2008-08-07 | The Ohio Sate University Research Foundation | System for expression of genes in plants from a virus-based expression vector |
| MX2009011893A (es) * | 2007-05-09 | 2010-02-17 | Devgen Nv | Metodo para la transcripcion sustentable de transgenes. |
| US8513397B2 (en) * | 2008-08-27 | 2013-08-20 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants |
| CA3015426C (en) | 2008-11-18 | 2020-01-14 | Commonwealth Scientific And Industrial Research Organisation | Recombinant cells comprising exogenous .delta.5 and .delta.6 desaturases, .delta.5 and .delta.6 elongases, and .delta.4 desaturases |
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| WO2010099462A2 (en) * | 2009-02-26 | 2010-09-02 | Baylor University | Highly efficient suppressor-dependent protein expression in plants with a viral vector |
| US20110197312A1 (en) * | 2010-02-07 | 2011-08-11 | University Of Kuwait | Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi |
| FR2960550A1 (fr) * | 2010-05-26 | 2011-12-02 | Inst Rech R Pour Le Dev Ird | Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique |
| FI20100248A0 (fi) | 2010-06-11 | 2010-06-11 | Kristiina Maekinen | Menetelmä proteiinien tuottamiseksi |
| CA2824152C (en) | 2011-01-17 | 2022-12-13 | Philip Morris Products S.A. | Method and system for producing a heterologous polypeptide in a nicotiana tabacum plant |
| WO2012098111A1 (en) | 2011-01-17 | 2012-07-26 | Philip Morris Products S.A. | Vectors for nucleic acid expression in plants |
| KR101334846B1 (ko) * | 2011-07-20 | 2013-11-29 | 대한민국 | 벼줄무늬잎마름병 바이러스 유래 ｎｓ3유전자의 신규 용도 |
| WO2013096992A1 (en) * | 2011-12-27 | 2013-07-04 | Commonwealth Scientific And Industrial Reserach Organisation | Simultaneous gene silencing and supressing gene silencing in the same cell |
| HUE033766T2 (en) | 2012-06-15 | 2018-01-29 | Commw Scient Ind Res Org | Production of long chain polyunsaturated fatty acids in plant cells |
| EA037817B1 (ru) | 2013-12-18 | 2021-05-25 | Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн | Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты |
| WO2015168733A1 (en) * | 2014-05-05 | 2015-11-12 | Queensland University Of Technology | Decoy molecules |
| CA2953008C (en) | 2014-06-27 | 2024-03-19 | Nuseed Pty Ltd | Lipid comprising docosapentaenoic acid |
| WO2019049111A1 (en) | 2017-09-11 | 2019-03-14 | R. J. Reynolds Tobacco Company | METHODS AND COMPOSITIONS FOR INCREASING THE EXPRESSION OF GENES OF INTEREST IN A PLANT BY CO-EXPRESSION WITH P21 |
| US20220002746A1 (en) * | 2018-11-13 | 2022-01-06 | University Of Maryland, College Park | Plant Vectors, Compositions and Uses Relating Thereto |
| CN115851820B (zh) * | 2022-12-19 | 2024-06-18 | 内蒙古农业大学 | 一株烟草脉斑驳病毒的应用 |
| CN119410670A (zh) * | 2025-01-06 | 2025-02-11 | 中国热带农业科学院三亚研究院 | 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO1998044097A2 (en) * | 1997-03-28 | 1998-10-08 | Vance Vicki B | Method for enhancing expression of a foreign or endogenous gene product in plants |
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| US6972349B1 (en) * | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| CA2390049C (en) * | 1999-11-12 | 2012-01-24 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
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| US8871997B2 (en) | 2014-10-28 |
| US7217854B1 (en) | 2007-05-15 |
| KR20030004305A (ko) | 2003-01-14 |
| JP2003514585A (ja) | 2003-04-22 |
| ATE460490T1 (de) | 2010-03-15 |
| AU782788B2 (en) | 2005-08-25 |
| IL149388A0 (en) | 2002-11-10 |
| WO2001038512A3 (en) | 2002-03-28 |
| AU1533401A (en) | 2001-06-04 |
| CA2390152A1 (en) | 2001-05-31 |
| EP1232274A2 (en) | 2002-08-21 |
| CA2390152C (en) | 2012-08-28 |
| EP1232274B1 (en) | 2010-03-10 |
| DE60043992D1 (de) | 2010-04-22 |
| WO2001038512A2 (en) | 2001-05-31 |
| MXPA02005089A (es) | 2003-01-28 |
| NZ518947A (en) | 2004-05-28 |
| US20070292862A1 (en) | 2007-12-20 |
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