ATE460490T1 - Erhöhte expression von transgenen durch co- exprimierung mit einen suppressor der post- transkriptionelle genabschaltung (ptgs) - Google Patents
Erhöhte expression von transgenen durch co- exprimierung mit einen suppressor der post- transkriptionelle genabschaltung (ptgs)Info
- Publication number
- ATE460490T1 ATE460490T1 AT00977696T AT00977696T ATE460490T1 AT E460490 T1 ATE460490 T1 AT E460490T1 AT 00977696 T AT00977696 T AT 00977696T AT 00977696 T AT00977696 T AT 00977696T AT E460490 T1 ATE460490 T1 AT E460490T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- expression
- plant
- ptgs
- nucleic acid
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 102000004169 proteins and genes Human genes 0.000 abstract 5
- 241000196324 Embryophyta Species 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 239000013598 vector Substances 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 230000032361 posttranscriptional gene silencing Effects 0.000 abstract 3
- 230000014509 gene expression Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 241000702449 African cassava mosaic virus Species 0.000 abstract 1
- 101000846901 Drosophila melanogaster Fat-body protein 1 Proteins 0.000 abstract 1
- 241000709992 Potato virus X Species 0.000 abstract 1
- 102100029008 Putative HTLV-1-related endogenous sequence Human genes 0.000 abstract 1
- 241001429314 Rice yellow mottle virus Species 0.000 abstract 1
- 241000710145 Tomato bushy stunt virus Species 0.000 abstract 1
- 108700019146 Transgenes Proteins 0.000 abstract 1
- 230000003362 replicative effect Effects 0.000 abstract 1
- 230000010474 transient expression Effects 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9927609.9A GB9927609D0 (en) | 1999-11-22 | 1999-11-22 | Enhanced expression |
| GB0022960A GB0022960D0 (en) | 2000-09-19 | 2000-09-19 | Enhanced expression |
| GB0023668A GB0023668D0 (en) | 2000-09-27 | 2000-09-27 | Enhanced expression |
| PCT/GB2000/004454 WO2001038512A2 (en) | 1999-11-22 | 2000-11-22 | Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE460490T1 true ATE460490T1 (de) | 2010-03-15 |
Family
ID=27255894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00977696T ATE460490T1 (de) | 1999-11-22 | 2000-11-22 | Erhöhte expression von transgenen durch co- exprimierung mit einen suppressor der post- transkriptionelle genabschaltung (ptgs) |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7217854B1 (de) |
| EP (1) | EP1232274B1 (de) |
| JP (1) | JP4884628B2 (de) |
| KR (1) | KR20030004305A (de) |
| AT (1) | ATE460490T1 (de) |
| AU (1) | AU782788B2 (de) |
| CA (1) | CA2390152C (de) |
| DE (1) | DE60043992D1 (de) |
| IL (1) | IL149388A0 (de) |
| MX (1) | MXPA02005089A (de) |
| NZ (1) | NZ518947A (de) |
| WO (1) | WO2001038512A2 (de) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2390049C (en) * | 1999-11-12 | 2012-01-24 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| US6972349B1 (en) | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| GB0101505D0 (en) * | 2001-01-19 | 2001-03-07 | Scottish Crop Res Inst | Protection against gene silencing |
| GB0101513D0 (en) * | 2001-01-19 | 2001-03-07 | Scottish Crop Res Inst | 'Gene silencing suppressor' |
| CA2492917C (en) * | 2002-07-19 | 2011-10-18 | University Of South Carolina | Compositions and methods for the modulation of gene expression in plants |
| US7692063B2 (en) | 2002-11-12 | 2010-04-06 | Ibio, Inc. | Production of foreign nucleic acids and polypeptides in sprout systems |
| US7683238B2 (en) | 2002-11-12 | 2010-03-23 | iBio, Inc. and Fraunhofer USA, Inc. | Production of pharmaceutically active proteins in sprouted seedlings |
| AU2004209660A1 (en) | 2003-02-03 | 2004-08-19 | Fraunhofer Usa, Inc. | System for expression of genes in plants |
| WO2005033306A1 (ja) * | 2003-10-01 | 2005-04-14 | Japan Science And Technology Agency | 形質転換細胞および該細胞を用いたタンパク質の生産方法並びにタンパク質生産キット、ウイルスベクター発現用ｄｎａ断片およびその利用、タンパク質生産用形質転換体の生産方法および当該生産方法によって得られたタンパク質生産用形質転換体並びにその利用 |
| GB0327919D0 (en) * | 2003-12-02 | 2004-01-07 | Plant Bioscience Ltd | Enhanced expression |
| AU2005216133A1 (en) * | 2004-02-20 | 2005-09-09 | Fraunhofer Usa, Inc. | Systems and methods for clonal expression in plants |
| DK1756280T3 (en) | 2004-04-22 | 2015-02-02 | Commw Scient Ind Res Org | SYNTHESIS OF CHAIN, polyunsaturated fatty acids BY RECOMBINANT CELLS |
| CA3023314C (en) | 2004-04-22 | 2019-12-10 | Commonwealth Scientific And Industrial Research Organisation | Synthesis of long-chain polyunsaturated fatty acids by recombinant cells |
| EP1616959A1 (de) * | 2004-07-07 | 2006-01-18 | Icon Genetics AG | Biologisch sichere transiente Proteinexpression in Pflanzen |
| FR2877014B1 (fr) * | 2004-10-21 | 2009-07-10 | Inst Rech Pour Le Dev I R D Et | Systemes d'expression de proteines recombinantes et leurs applications |
| FR2880357B1 (fr) * | 2005-01-05 | 2010-12-10 | Inst Rech Developpement Ird | Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications |
| WO2007092460A2 (en) * | 2006-02-03 | 2007-08-16 | The Texas A & M University System | Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants |
| AU2007291937B2 (en) | 2006-08-29 | 2014-05-15 | Commonwealth Scientific And Industrial Research Organisation | Synthesis of fatty acids |
| EP1897953A1 (de) * | 2006-09-06 | 2008-03-12 | Icon Genetics GmbH | Replicon von Potexviren stammend |
| US9605270B2 (en) * | 2006-09-22 | 2017-03-28 | The Regents Of The University Of California | Chemically inducible cucumber mosaic virus protein expression system |
| WO2008094512A2 (en) * | 2007-01-29 | 2008-08-07 | The Ohio Sate University Research Foundation | System for expression of genes in plants from a virus-based expression vector |
| WO2008138896A1 (en) * | 2007-05-09 | 2008-11-20 | Devgen N.V. | A method for sustainable transgene transcription |
| AU2009285667B2 (en) * | 2008-08-27 | 2015-05-28 | Arizona Board Of Regents For And On Behalf Of Arizona State University | A DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants |
| CA3015426C (en) | 2008-11-18 | 2020-01-14 | Commonwealth Scientific And Industrial Research Organisation | Recombinant cells comprising exogenous .delta.5 and .delta.6 desaturases, .delta.5 and .delta.6 elongases, and .delta.4 desaturases |
| AU2013204254B8 (en) * | 2008-11-18 | 2015-10-08 | Commonwealth Scientific And Industrial Research Organisation | Enzymes and methods for producing omega-3 fatty acids |
| WO2010099462A2 (en) * | 2009-02-26 | 2010-09-02 | Baylor University | Highly efficient suppressor-dependent protein expression in plants with a viral vector |
| US20110197312A1 (en) * | 2010-02-07 | 2011-08-11 | University Of Kuwait | Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi |
| FR2960550A1 (fr) * | 2010-05-26 | 2011-12-02 | Inst Rech R Pour Le Dev Ird | Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique |
| FI20100248A0 (fi) | 2010-06-11 | 2010-06-11 | Kristiina Maekinen | Menetelmä proteiinien tuottamiseksi |
| PH12013501459A1 (en) | 2011-01-17 | 2013-08-28 | Philip Morris Products Sa | Protein expression in plants |
| US20140059718A1 (en) | 2011-01-17 | 2014-02-27 | Philip Morris Products S.A. | Vectors for nucleic acid expression in plants |
| KR101334846B1 (ko) * | 2011-07-20 | 2013-11-29 | 대한민국 | 벼줄무늬잎마름병 바이러스 유래 ｎｓ3유전자의 신규 용도 |
| ES2744240T3 (es) * | 2011-12-27 | 2020-02-24 | Commw Scient Ind Res Org | Silenciamiento génico y supresión del silenciamiento génico simultáneos en la misma célula |
| NZ631702A (en) | 2012-06-15 | 2017-01-27 | Grains Res & Dev Corp | Production of long chain polyunsaturated fatty acids in plant cells |
| EP3082405A4 (de) | 2013-12-18 | 2017-12-13 | Commonwealth Scientific and Industrial Research Organisation | Lipid mit langkettigen mehrfach ungesättigten fettsäuren |
| WO2015168733A1 (en) * | 2014-05-05 | 2015-11-12 | Queensland University Of Technology | Decoy molecules |
| PH12016502586B1 (en) | 2014-06-27 | 2023-07-19 | Commw Scient Ind Res Org | Lipid comprising docosapentaenoic acid |
| WO2019049111A1 (en) | 2017-09-11 | 2019-03-14 | R. J. Reynolds Tobacco Company | METHODS AND COMPOSITIONS FOR INCREASING THE EXPRESSION OF GENES OF INTEREST IN A PLANT BY CO-EXPRESSION WITH P21 |
| US20220002746A1 (en) * | 2018-11-13 | 2022-01-06 | University Of Maryland, College Park | Plant Vectors, Compositions and Uses Relating Thereto |
| CN115851820B (zh) * | 2022-12-19 | 2024-06-18 | 内蒙古农业大学 | 一株烟草脉斑驳病毒的应用 |
| CN119410670A (zh) * | 2025-01-06 | 2025-02-11 | 中国热带农业科学院三亚研究院 | 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5939541A (en) * | 1997-03-28 | 1999-08-17 | University Of South Carolina | Method for enhancing expression of a foreign or endogenous gene product in plants |
| US6972349B1 (en) * | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| CA2390049C (en) * | 1999-11-12 | 2012-01-24 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
-
2000
- 2000-11-22 AU AU15334/01A patent/AU782788B2/en not_active Ceased
- 2000-11-22 MX MXPA02005089A patent/MXPA02005089A/es unknown
- 2000-11-22 US US10/130,758 patent/US7217854B1/en not_active Expired - Lifetime
- 2000-11-22 CA CA2390152A patent/CA2390152C/en not_active Expired - Lifetime
- 2000-11-22 WO PCT/GB2000/004454 patent/WO2001038512A2/en not_active Ceased
- 2000-11-22 DE DE60043992T patent/DE60043992D1/de not_active Expired - Lifetime
- 2000-11-22 JP JP2001540262A patent/JP4884628B2/ja not_active Expired - Fee Related
- 2000-11-22 IL IL14938800A patent/IL149388A0/xx not_active IP Right Cessation
- 2000-11-22 EP EP00977696A patent/EP1232274B1/de not_active Expired - Lifetime
- 2000-11-22 AT AT00977696T patent/ATE460490T1/de not_active IP Right Cessation
- 2000-11-22 KR KR1020027006548A patent/KR20030004305A/ko not_active Ceased
- 2000-11-22 NZ NZ518947A patent/NZ518947A/en unknown
-
2006
- 2006-11-20 US US11/602,416 patent/US8871997B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003514585A (ja) | 2003-04-22 |
| US7217854B1 (en) | 2007-05-15 |
| AU782788B2 (en) | 2005-08-25 |
| DE60043992D1 (de) | 2010-04-22 |
| CA2390152C (en) | 2012-08-28 |
| JP4884628B2 (ja) | 2012-02-29 |
| AU1533401A (en) | 2001-06-04 |
| US8871997B2 (en) | 2014-10-28 |
| NZ518947A (en) | 2004-05-28 |
| US20070292862A1 (en) | 2007-12-20 |
| MXPA02005089A (es) | 2003-01-28 |
| CA2390152A1 (en) | 2001-05-31 |
| EP1232274B1 (de) | 2010-03-10 |
| WO2001038512A3 (en) | 2002-03-28 |
| EP1232274A2 (de) | 2002-08-21 |
| IL149388A0 (en) | 2002-11-10 |
| KR20030004305A (ko) | 2003-01-14 |
| WO2001038512A2 (en) | 2001-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |