DK0724639T3 - Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket protein - Google Patents
Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket proteinInfo
- Publication number
- DK0724639T3 DK0724639T3 DK92908635T DK92908635T DK0724639T3 DK 0724639 T3 DK0724639 T3 DK 0724639T3 DK 92908635 T DK92908635 T DK 92908635T DK 92908635 T DK92908635 T DK 92908635T DK 0724639 T3 DK0724639 T3 DK 0724639T3
- Authority
- DK
- Denmark
- Prior art keywords
- methods
- host cells
- high levels
- cells expressing
- desired protein
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67704591A | 1991-03-29 | 1991-03-29 | |
| PCT/US1992/001358 WO1992017566A2 (en) | 1991-03-29 | 1992-02-20 | Methods for selection of recombinant host cells expressing high levels of a desired protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0724639T3 true DK0724639T3 (da) | 2001-03-05 |
Family
ID=24717083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK92908635T DK0724639T3 (da) | 1991-03-29 | 1992-02-20 | Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket protein |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0724639B1 (da) |
| JP (1) | JP3438889B2 (da) |
| AT (1) | ATE199023T1 (da) |
| AU (1) | AU660671B2 (da) |
| CA (1) | CA2104598C (da) |
| DE (1) | DE69231676T2 (da) |
| DK (1) | DK0724639T3 (da) |
| ES (1) | ES2153358T3 (da) |
| GR (1) | GR3035599T3 (da) |
| WO (1) | WO1992017566A2 (da) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5561053A (en) * | 1994-08-05 | 1996-10-01 | Genentech, Inc. | Method for selecting high-expressing host cells |
| WO2003048306A2 (en) | 2001-11-16 | 2003-06-12 | Idec Pharmaceuticals Corporation | Polycistronic expression of antibodies |
| USRE47770E1 (en) | 2002-07-18 | 2019-12-17 | Merus N.V. | Recombinant production of mixtures of antibodies |
| SI1523496T1 (sl) | 2002-07-18 | 2011-11-30 | Merus B V | Rekombinantno proizvajanje zmesi protiteles |
| DK1572994T3 (da) | 2002-12-20 | 2007-05-29 | Chromagenics Bv | Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer |
| US20100069614A1 (en) | 2008-06-27 | 2010-03-18 | Merus B.V. | Antibody producing non-human mammals |
| AU2004242614B2 (en) | 2003-05-30 | 2011-09-22 | Merus N.V. | Fab library for the preparation of anti vegf and anti rabies virus fabs |
| EP1737971B1 (en) | 2004-01-20 | 2017-08-16 | Merus N.V. | Mixtures of binding proteins |
| ES2384391T3 (es) | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
| US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| JP5291341B2 (ja) | 2004-11-08 | 2013-09-18 | クロマジェニックス ベー ヴェー | タンパク質を高レベルで発現する宿主細胞の選定 |
| CN104342454A (zh) | 2008-12-22 | 2015-02-11 | 国立大学法人北海道大学 | 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用 |
| SG173456A1 (en) | 2009-02-27 | 2011-09-29 | Novartis Ag | Expression vector system comprising two selection markers |
| EA035344B1 (ru) | 2012-04-20 | 2020-05-29 | Мерюс Н.В. | Способ получения двух антител из одной клетки-хозяина |
| EP3412684B1 (en) | 2013-07-31 | 2022-04-06 | Novartis AG | Novel selection vectors and methods of selecting eukaryotic host cells |
| RU2712507C2 (ru) | 2013-12-20 | 2020-01-29 | Новартис Аг | Новые эукариотические клетки и способы их получения для рекомбинантной экспрессии представляющего интерес продукта |
| CN106029692A (zh) | 2013-12-20 | 2016-10-12 | 诺华股份有限公司 | 用于重组表达感兴趣产物的新型真核细胞和方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2353384A (en) * | 1983-01-19 | 1984-07-26 | Genentech Inc. | Amplification in eukaryotic host cells |
| US5043270A (en) * | 1989-03-31 | 1991-08-27 | The Board Of Trustees Of The Leland Stanford Junior University | Intronic overexpression vectors |
-
1992
- 1992-02-20 AU AU15806/92A patent/AU660671B2/en not_active Expired
- 1992-02-20 EP EP92908635A patent/EP0724639B1/en not_active Expired - Lifetime
- 1992-02-20 DE DE69231676T patent/DE69231676T2/de not_active Expired - Lifetime
- 1992-02-20 WO PCT/US1992/001358 patent/WO1992017566A2/en active IP Right Grant
- 1992-02-20 CA CA002104598A patent/CA2104598C/en not_active Expired - Lifetime
- 1992-02-20 ES ES92908635T patent/ES2153358T3/es not_active Expired - Lifetime
- 1992-02-20 JP JP50818192A patent/JP3438889B2/ja not_active Expired - Lifetime
- 1992-02-20 AT AT92908635T patent/ATE199023T1/de active
- 1992-02-20 DK DK92908635T patent/DK0724639T3/da active
-
2001
- 2001-03-20 GR GR20010400445T patent/GR3035599T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GR3035599T3 (en) | 2001-06-29 |
| EP0724639A1 (en) | 1996-08-07 |
| WO1992017566A2 (en) | 1992-10-15 |
| ES2153358T3 (es) | 2001-03-01 |
| AU1580692A (en) | 1992-11-02 |
| JP3438889B2 (ja) | 2003-08-18 |
| DE69231676D1 (de) | 2001-03-08 |
| DE69231676T2 (de) | 2001-05-17 |
| JPH06506356A (ja) | 1994-07-21 |
| WO1992017566A3 (en) | 1993-01-21 |
| CA2104598C (en) | 2003-01-07 |
| CA2104598A1 (en) | 1992-09-30 |
| ATE199023T1 (de) | 2001-02-15 |
| AU660671B2 (en) | 1995-07-06 |
| EP0724639B1 (en) | 2001-01-31 |
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