ES2153358T3 - Procedimientos para la seleccion de celulas huespedes recombinantes que expresan altos niveles de una proteina deseada. - Google Patents
Procedimientos para la seleccion de celulas huespedes recombinantes que expresan altos niveles de una proteina deseada.Info
- Publication number
- ES2153358T3 ES2153358T3 ES92908635T ES92908635T ES2153358T3 ES 2153358 T3 ES2153358 T3 ES 2153358T3 ES 92908635 T ES92908635 T ES 92908635T ES 92908635 T ES92908635 T ES 92908635T ES 2153358 T3 ES2153358 T3 ES 2153358T3
- Authority
- ES
- Spain
- Prior art keywords
- selection
- high levels
- recombinant
- desired protein
- procedures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Endocrinology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
LA PRESENTE INVENCION SE REFIERE A METODOS MEJORADOS PARA LA SELECCION DE CELULAS HUESPED RECOMBINANTES QUE EXPRESEN ALTOS NIVELES DE UNA PROTEINA DESEADA, LOS CUALES SON UTILES CON UNA AMPLIA VARIEDAD DE CELULAS HUESPED EUCARIOTICAS Y EVITAN LOS PROBLEMAS INHERENTES EN TECNOLOGIA DE SELECCION CELULAR EXISTENTE. LA INVENCION ADEMAS SE REFIERE A GENES QUE SE PUEDEN SELECCIONAR MODIFICADOS CON INTRONA, QUE COMPRENDEN LA SECUENCIA DE CODIFICACION DE UN GEN SELECCIONABLE Y UNA INTRONA QUE REDUCE EL NIVEL DE PROTEINA SELECCIONABLE PRODUCIDA A PARTIR DEL GEN SELECCIONABLE EN UNA CELULA HUESPED, LOS CUALES SON UTILES EN LOS METODOS DE LA INVENCION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US67704591A | 1991-03-29 | 1991-03-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2153358T3 true ES2153358T3 (es) | 2001-03-01 |
Family
ID=24717083
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES92908635T Expired - Lifetime ES2153358T3 (es) | 1991-03-29 | 1992-02-20 | Procedimientos para la seleccion de celulas huespedes recombinantes que expresan altos niveles de una proteina deseada. |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0724639B1 (es) |
JP (1) | JP3438889B2 (es) |
AT (1) | ATE199023T1 (es) |
AU (1) | AU660671B2 (es) |
CA (1) | CA2104598C (es) |
DE (1) | DE69231676T2 (es) |
DK (1) | DK0724639T3 (es) |
ES (1) | ES2153358T3 (es) |
GR (1) | GR3035599T3 (es) |
WO (1) | WO1992017566A2 (es) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5561053A (en) * | 1994-08-05 | 1996-10-01 | Genentech, Inc. | Method for selecting high-expressing host cells |
EA007905B1 (ru) | 2001-11-16 | 2007-02-27 | Байоджен Айдек Инк. | Полицистронная экспрессия антител |
USRE47770E1 (en) | 2002-07-18 | 2019-12-17 | Merus N.V. | Recombinant production of mixtures of antibodies |
ATE514717T1 (de) | 2002-07-18 | 2011-07-15 | Merus B V | Rekombinante produktion von antikörpermischungen |
EP1572994B1 (en) | 2002-12-20 | 2007-02-21 | Chromagenics B.V. | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors |
US20100069614A1 (en) | 2008-06-27 | 2010-03-18 | Merus B.V. | Antibody producing non-human mammals |
WO2004106375A1 (en) | 2003-05-30 | 2004-12-09 | Merus Biopharmaceuticals B.V. I.O. | Fab library for the preparation of anti vegf and anti rabies virus fabs |
CA2554054C (en) | 2004-01-20 | 2013-06-04 | Merus B.V. | Mixtures of binding proteins |
EA010863B1 (ru) | 2004-11-08 | 2008-12-30 | Хромагеникс Б.В. | Отбор клеток-хозяев, экспрессирующих белок на высоких уровнях |
DK1809750T3 (da) | 2004-11-08 | 2012-06-25 | Chromagenics Bv | Selektion af værtsceller, der udtrykker protein ved høje niveauer |
US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
JP5704753B2 (ja) * | 2008-12-22 | 2015-04-22 | 国立大学法人北海道大学 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
EP2401377B1 (en) | 2009-02-27 | 2016-05-18 | Novartis AG | Expression vector system comprising two selection markers |
PT2838917T (pt) | 2012-04-20 | 2019-09-12 | Merus Nv | Métodos e meios para a produção de moléculas similares a ig heterodiméricas |
AU2014297966B2 (en) | 2013-07-31 | 2017-10-26 | Novartis Ag | Novel selection vectors and methods of selecting eukaryotic host cells |
US11242551B2 (en) | 2013-12-20 | 2022-02-08 | Novartis Ag | Eukaryotic cells and methods for recombinantly expressing a product of interest |
SG11201604215XA (en) | 2013-12-20 | 2016-07-28 | Novartis Ag | Novel eukaryotic cells and methods for recombinantly expressing a product of interest |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2353384A (en) * | 1983-01-19 | 1984-07-26 | Genentech Inc. | Amplification in eukaryotic host cells |
US5043270A (en) * | 1989-03-31 | 1991-08-27 | The Board Of Trustees Of The Leland Stanford Junior University | Intronic overexpression vectors |
-
1992
- 1992-02-20 AU AU15806/92A patent/AU660671B2/en not_active Expired
- 1992-02-20 CA CA002104598A patent/CA2104598C/en not_active Expired - Lifetime
- 1992-02-20 EP EP92908635A patent/EP0724639B1/en not_active Expired - Lifetime
- 1992-02-20 AT AT92908635T patent/ATE199023T1/de active
- 1992-02-20 WO PCT/US1992/001358 patent/WO1992017566A2/en active IP Right Grant
- 1992-02-20 DK DK92908635T patent/DK0724639T3/da active
- 1992-02-20 JP JP50818192A patent/JP3438889B2/ja not_active Expired - Lifetime
- 1992-02-20 DE DE69231676T patent/DE69231676T2/de not_active Expired - Lifetime
- 1992-02-20 ES ES92908635T patent/ES2153358T3/es not_active Expired - Lifetime
-
2001
- 2001-03-20 GR GR20010400445T patent/GR3035599T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
AU1580692A (en) | 1992-11-02 |
CA2104598C (en) | 2003-01-07 |
DK0724639T3 (da) | 2001-03-05 |
WO1992017566A2 (en) | 1992-10-15 |
WO1992017566A3 (en) | 1993-01-21 |
EP0724639A1 (en) | 1996-08-07 |
JPH06506356A (ja) | 1994-07-21 |
DE69231676T2 (de) | 2001-05-17 |
JP3438889B2 (ja) | 2003-08-18 |
ATE199023T1 (de) | 2001-02-15 |
DE69231676D1 (de) | 2001-03-08 |
CA2104598A1 (en) | 1992-09-30 |
AU660671B2 (en) | 1995-07-06 |
EP0724639B1 (en) | 2001-01-31 |
GR3035599T3 (en) | 2001-06-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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