DE60043992D1 - Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs) - Google Patents
Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs)Info
- Publication number
- DE60043992D1 DE60043992D1 DE60043992T DE60043992T DE60043992D1 DE 60043992 D1 DE60043992 D1 DE 60043992D1 DE 60043992 T DE60043992 T DE 60043992T DE 60043992 T DE60043992 T DE 60043992T DE 60043992 D1 DE60043992 D1 DE 60043992D1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- plant
- expression
- ptgs
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000014509 gene expression Effects 0.000 title abstract 3
- 108700019146 Transgenes Proteins 0.000 title abstract 2
- 230000004186 co-expression Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 102000004169 proteins and genes Human genes 0.000 abstract 5
- 241000196324 Embryophyta Species 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 239000013598 vector Substances 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 230000032361 posttranscriptional gene silencing Effects 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 241000702449 African cassava mosaic virus Species 0.000 abstract 1
- 101000846901 Drosophila melanogaster Fat-body protein 1 Proteins 0.000 abstract 1
- 241000709992 Potato virus X Species 0.000 abstract 1
- 102100029008 Putative HTLV-1-related endogenous sequence Human genes 0.000 abstract 1
- 241001429314 Rice yellow mottle virus Species 0.000 abstract 1
- 241000710145 Tomato bushy stunt virus Species 0.000 abstract 1
- 230000003362 replicative effect Effects 0.000 abstract 1
- 230000010474 transient expression Effects 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9927609.9A GB9927609D0 (en) | 1999-11-22 | 1999-11-22 | Enhanced expression |
| GB0022960A GB0022960D0 (en) | 2000-09-19 | 2000-09-19 | Enhanced expression |
| GB0023668A GB0023668D0 (en) | 2000-09-27 | 2000-09-27 | Enhanced expression |
| PCT/GB2000/004454 WO2001038512A2 (en) | 1999-11-22 | 2000-11-22 | Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60043992D1 true DE60043992D1 (de) | 2010-04-22 |
Family
ID=27255894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60043992T Expired - Lifetime DE60043992D1 (de) | 1999-11-22 | 2000-11-22 | Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs) |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7217854B1 (enExample) |
| EP (1) | EP1232274B1 (enExample) |
| JP (1) | JP4884628B2 (enExample) |
| KR (1) | KR20030004305A (enExample) |
| AT (1) | ATE460490T1 (enExample) |
| AU (1) | AU782788B2 (enExample) |
| CA (1) | CA2390152C (enExample) |
| DE (1) | DE60043992D1 (enExample) |
| IL (1) | IL149388A0 (enExample) |
| MX (1) | MXPA02005089A (enExample) |
| NZ (1) | NZ518947A (enExample) |
| WO (1) | WO2001038512A2 (enExample) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6972349B1 (en) | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| CA2390049C (en) * | 1999-11-12 | 2012-01-24 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| GB0101505D0 (en) * | 2001-01-19 | 2001-03-07 | Scottish Crop Res Inst | Protection against gene silencing |
| GB0101513D0 (en) * | 2001-01-19 | 2001-03-07 | Scottish Crop Res Inst | 'Gene silencing suppressor' |
| CA2492917C (en) | 2002-07-19 | 2011-10-18 | University Of South Carolina | Compositions and methods for the modulation of gene expression in plants |
| US7683238B2 (en) | 2002-11-12 | 2010-03-23 | iBio, Inc. and Fraunhofer USA, Inc. | Production of pharmaceutically active proteins in sprouted seedlings |
| US7692063B2 (en) | 2002-11-12 | 2010-04-06 | Ibio, Inc. | Production of foreign nucleic acids and polypeptides in sprout systems |
| EP1594956A4 (en) | 2003-02-03 | 2007-08-01 | Fraunhofer Usa Inc | SYSTEM FOR EXPRESSION OF GENES IN PLANTS |
| AU2004278624B2 (en) * | 2003-10-01 | 2008-03-20 | Japan Science And Technology Agency | DNA fragment, method for producing transformant for protein production and utilization thereof |
| GB0327919D0 (en) * | 2003-12-02 | 2004-01-07 | Plant Bioscience Ltd | Enhanced expression |
| ES2532609T3 (es) * | 2004-02-20 | 2015-03-30 | Ibio, Inc. | Sistemas y métodos para expresión clonales en plantas |
| PL1756280T3 (pl) | 2004-04-22 | 2015-04-30 | Commw Scient Ind Res Org | Synteza długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach rekombinowanych |
| ES2529572T3 (es) | 2004-04-22 | 2015-02-23 | Commonwealth Scientific And Industrial Research Organisation | Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes |
| EP1616959A1 (en) * | 2004-07-07 | 2006-01-18 | Icon Genetics AG | Biological safe transient protein expression in plants |
| FR2877014B1 (fr) * | 2004-10-21 | 2009-07-10 | Inst Rech Pour Le Dev I R D Et | Systemes d'expression de proteines recombinantes et leurs applications |
| FR2880357B1 (fr) * | 2005-01-05 | 2010-12-10 | Inst Rech Developpement Ird | Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications |
| WO2007092460A2 (en) * | 2006-02-03 | 2007-08-16 | The Texas A & M University System | Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants |
| BRPI0716075A2 (pt) | 2006-08-29 | 2013-08-06 | Commw Scient Ind Res Org | sÍntese de Ácidos graxos |
| EP1897953A1 (en) * | 2006-09-06 | 2008-03-12 | Icon Genetics GmbH | Potexvirus-derived replicon |
| US9605270B2 (en) * | 2006-09-22 | 2017-03-28 | The Regents Of The University Of California | Chemically inducible cucumber mosaic virus protein expression system |
| WO2008094512A2 (en) | 2007-01-29 | 2008-08-07 | The Ohio Sate University Research Foundation | System for expression of genes in plants from a virus-based expression vector |
| MX2009011893A (es) * | 2007-05-09 | 2010-02-17 | Devgen Nv | Metodo para la transcripcion sustentable de transgenes. |
| US8513397B2 (en) * | 2008-08-27 | 2013-08-20 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants |
| CA3015426C (en) | 2008-11-18 | 2020-01-14 | Commonwealth Scientific And Industrial Research Organisation | Recombinant cells comprising exogenous .delta.5 and .delta.6 desaturases, .delta.5 and .delta.6 elongases, and .delta.4 desaturases |
| AU2013204254B8 (en) * | 2008-11-18 | 2015-10-08 | Commonwealth Scientific And Industrial Research Organisation | Enzymes and methods for producing omega-3 fatty acids |
| WO2010099462A2 (en) * | 2009-02-26 | 2010-09-02 | Baylor University | Highly efficient suppressor-dependent protein expression in plants with a viral vector |
| US20110197312A1 (en) * | 2010-02-07 | 2011-08-11 | University Of Kuwait | Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi |
| FR2960550A1 (fr) * | 2010-05-26 | 2011-12-02 | Inst Rech R Pour Le Dev Ird | Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique |
| FI20100248A0 (fi) | 2010-06-11 | 2010-06-11 | Kristiina Maekinen | Menetelmä proteiinien tuottamiseksi |
| CA2824152C (en) | 2011-01-17 | 2022-12-13 | Philip Morris Products S.A. | Method and system for producing a heterologous polypeptide in a nicotiana tabacum plant |
| WO2012098111A1 (en) | 2011-01-17 | 2012-07-26 | Philip Morris Products S.A. | Vectors for nucleic acid expression in plants |
| KR101334846B1 (ko) * | 2011-07-20 | 2013-11-29 | 대한민국 | 벼줄무늬잎마름병 바이러스 유래 ｎｓ3유전자의 신규 용도 |
| WO2013096992A1 (en) * | 2011-12-27 | 2013-07-04 | Commonwealth Scientific And Industrial Reserach Organisation | Simultaneous gene silencing and supressing gene silencing in the same cell |
| HUE033766T2 (en) | 2012-06-15 | 2018-01-29 | Commw Scient Ind Res Org | Production of long chain polyunsaturated fatty acids in plant cells |
| EA037817B1 (ru) | 2013-12-18 | 2021-05-25 | Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн | Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты |
| WO2015168733A1 (en) * | 2014-05-05 | 2015-11-12 | Queensland University Of Technology | Decoy molecules |
| CA2953008C (en) | 2014-06-27 | 2024-03-19 | Nuseed Pty Ltd | Lipid comprising docosapentaenoic acid |
| WO2019049111A1 (en) | 2017-09-11 | 2019-03-14 | R. J. Reynolds Tobacco Company | METHODS AND COMPOSITIONS FOR INCREASING THE EXPRESSION OF GENES OF INTEREST IN A PLANT BY CO-EXPRESSION WITH P21 |
| US20220002746A1 (en) * | 2018-11-13 | 2022-01-06 | University Of Maryland, College Park | Plant Vectors, Compositions and Uses Relating Thereto |
| CN115851820B (zh) * | 2022-12-19 | 2024-06-18 | 内蒙古农业大学 | 一株烟草脉斑驳病毒的应用 |
| CN119410670A (zh) * | 2025-01-06 | 2025-02-11 | 中国热带农业科学院三亚研究院 | 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5939541A (en) * | 1997-03-28 | 1999-08-17 | University Of South Carolina | Method for enhancing expression of a foreign or endogenous gene product in plants |
| US6972349B1 (en) * | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| CA2390049C (en) * | 1999-11-12 | 2012-01-24 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
-
2000
- 2000-11-22 WO PCT/GB2000/004454 patent/WO2001038512A2/en not_active Ceased
- 2000-11-22 AU AU15334/01A patent/AU782788B2/en not_active Ceased
- 2000-11-22 JP JP2001540262A patent/JP4884628B2/ja not_active Expired - Fee Related
- 2000-11-22 MX MXPA02005089A patent/MXPA02005089A/es unknown
- 2000-11-22 CA CA2390152A patent/CA2390152C/en not_active Expired - Lifetime
- 2000-11-22 US US10/130,758 patent/US7217854B1/en not_active Expired - Lifetime
- 2000-11-22 KR KR1020027006548A patent/KR20030004305A/ko not_active Ceased
- 2000-11-22 EP EP00977696A patent/EP1232274B1/en not_active Expired - Lifetime
- 2000-11-22 AT AT00977696T patent/ATE460490T1/de not_active IP Right Cessation
- 2000-11-22 DE DE60043992T patent/DE60043992D1/de not_active Expired - Lifetime
- 2000-11-22 IL IL14938800A patent/IL149388A0/xx not_active IP Right Cessation
- 2000-11-22 NZ NZ518947A patent/NZ518947A/en unknown
-
2006
- 2006-11-20 US US11/602,416 patent/US8871997B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US8871997B2 (en) | 2014-10-28 |
| US7217854B1 (en) | 2007-05-15 |
| KR20030004305A (ko) | 2003-01-14 |
| JP2003514585A (ja) | 2003-04-22 |
| ATE460490T1 (de) | 2010-03-15 |
| AU782788B2 (en) | 2005-08-25 |
| IL149388A0 (en) | 2002-11-10 |
| JP4884628B2 (ja) | 2012-02-29 |
| WO2001038512A3 (en) | 2002-03-28 |
| AU1533401A (en) | 2001-06-04 |
| CA2390152A1 (en) | 2001-05-31 |
| EP1232274A2 (en) | 2002-08-21 |
| CA2390152C (en) | 2012-08-28 |
| EP1232274B1 (en) | 2010-03-10 |
| WO2001038512A2 (en) | 2001-05-31 |
| MXPA02005089A (es) | 2003-01-28 |
| NZ518947A (en) | 2004-05-28 |
| US20070292862A1 (en) | 2007-12-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |