KR102556363B1 - 보툴리눔 신경독소의 활성을 측정하기 위한 시험관내 및 세포 기반 검정 - Google Patents

보툴리눔 신경독소의 활성을 측정하기 위한 시험관내 및 세포 기반 검정 Download PDF

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KR102556363B1
KR102556363B1 KR1020197013429A KR20197013429A KR102556363B1 KR 102556363 B1 KR102556363 B1 KR 102556363B1 KR 1020197013429 A KR1020197013429 A KR 1020197013429A KR 20197013429 A KR20197013429 A KR 20197013429A KR 102556363 B1 KR102556363 B1 KR 102556363B1
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bont
luciferase
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neurotoxin
chain polypeptide
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KR20190068582A (ko
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민 동
페이판 위
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프레지던트 앤드 펠로우즈 오브 하바드 칼리지
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020197013429A 2016-10-20 2017-10-19 보툴리눔 신경독소의 활성을 측정하기 위한 시험관내 및 세포 기반 검정 Active KR102556363B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662410558P 2016-10-20 2016-10-20
US62/410,558 2016-10-20
PCT/US2017/057411 WO2018075783A2 (en) 2016-10-20 2017-10-19 In vitro and cell based assays for measuring the activity of botulinum neurotoxins

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KR20190068582A KR20190068582A (ko) 2019-06-18
KR102556363B1 true KR102556363B1 (ko) 2023-07-18

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US (2) US11306347B2 (https=)
EP (1) EP3529616B1 (https=)
JP (2) JP7227901B2 (https=)
KR (1) KR102556363B1 (https=)
CN (1) CN110088624B (https=)
AU (1) AU2017345560C1 (https=)
BR (1) BR112019007831A2 (https=)
CA (1) CA3040507A1 (https=)
DK (1) DK3529616T5 (https=)
EA (1) EA039761B1 (https=)
ES (1) ES2963830T3 (https=)
FI (1) FI3529616T3 (https=)
HU (1) HUE064332T2 (https=)
MX (2) MX2019004431A (https=)
PL (1) PL3529616T3 (https=)
PT (1) PT3529616T (https=)
SA (1) SA519401607B1 (https=)
SG (1) SG11201903523YA (https=)
UA (1) UA129533C2 (https=)
WO (1) WO2018075783A2 (https=)
ZA (1) ZA201902654B (https=)

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US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
WO2016168631A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Vector-based mutagenesis system
WO2017015545A1 (en) 2015-07-22 2017-01-26 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11524983B2 (en) 2015-07-23 2022-12-13 President And Fellows Of Harvard College Evolution of Bt toxins
US10612011B2 (en) 2015-07-30 2020-04-07 President And Fellows Of Harvard College Evolution of TALENs
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
US11242515B2 (en) * 2016-05-16 2022-02-08 President And Fellows Of Harvard College Method for purification and activation of botulinum neurotoxin
WO2018075783A2 (en) 2016-10-20 2018-04-26 President And Fellows Of Harvard College In vitro and cell based assays for measuring the activity of botulinum neurotoxins
US11447809B2 (en) 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
AU2018321405A1 (en) 2017-08-25 2020-03-05 Ipsen Biopharm Ltd. Evolution of BoNT peptidases
US11624130B2 (en) 2017-09-18 2023-04-11 President And Fellows Of Harvard College Continuous evolution for stabilized proteins
WO2019241649A1 (en) 2018-06-14 2019-12-19 President And Fellows Of Harvard College Evolution of cytidine deaminases
US20220259269A1 (en) * 2019-07-15 2022-08-18 President And Fellows Of Harvard College Evolved botulinum neurotoxins and uses thereof
WO2021195479A1 (en) * 2020-03-26 2021-09-30 Cellex, Inc. Protease assays and their applications
CN114540419A (zh) * 2022-03-04 2022-05-27 中国人民解放军军事科学院军事医学研究院 一种分析包膜病毒膜融合效率的三功能报告系统
GB202213479D0 (en) 2022-09-14 2022-10-26 Ipsen Biopharm Ltd Cell-free clostridial neurotoxin assays
WO2024217577A1 (zh) * 2023-04-21 2024-10-24 深圳琅技生命科技有限公司 一种肽以及含有该肽的发光复合物
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay
KR20250158928A (ko) * 2024-04-30 2025-11-07 주식회사 바이오링크스 앵커 단백질을 이용한 미광성 세포기반의 보툴리눔 독소의 검출 및 활성 평가방법
WO2026005306A1 (ko) * 2024-06-28 2026-01-02 ㈜바이오솔빅스 Snap-25를 발현하는 운동신경세포 제조 방법 및 제조된 운동신경세포를 이용한 보툴리눔 신경독소 역가 측정 방법
CN119039432B (zh) * 2024-10-31 2025-01-28 兰州生物技术开发有限公司 一种检测肉毒毒素活性或突触体相关蛋白的方法和试剂盒

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ES2963830T3 (es) 2024-04-02
PL3529616T3 (pl) 2024-03-18
CN110088624B (zh) 2024-01-16
WO2018075783A3 (en) 2018-06-21
AU2017345560C1 (en) 2025-04-17
CN110088624A (zh) 2019-08-02
EP3529616B1 (en) 2023-09-06
US20190276873A1 (en) 2019-09-12
SG11201903523YA (en) 2019-05-30
MX2019004431A (es) 2019-08-14
EA201990929A1 (ru) 2019-10-31
PT3529616T (pt) 2023-12-06
DK3529616T5 (da) 2024-09-02
US20220356508A1 (en) 2022-11-10
WO2018075783A2 (en) 2018-04-26
EA039761B1 (ru) 2022-03-10
EP3529616A2 (en) 2019-08-28
ZA201902654B (en) 2024-08-28
US11306347B2 (en) 2022-04-19
AU2017345560A1 (en) 2019-05-02
JP2023011700A (ja) 2023-01-24
UA129533C2 (uk) 2025-05-28
JP7227901B2 (ja) 2023-02-22
US11788069B2 (en) 2023-10-17
MX2023004733A (es) 2023-05-10
FI3529616T3 (fi) 2023-12-04
HUE064332T2 (hu) 2024-03-28
DK3529616T3 (da) 2023-12-11
BR112019007831A2 (pt) 2019-10-01
CA3040507A1 (en) 2018-04-26
JP2019532651A (ja) 2019-11-14
SA519401607B1 (ar) 2022-08-04
AU2017345560B2 (en) 2024-12-12
KR20190068582A (ko) 2019-06-18

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