KR102516695B1 - 단백질 내로의 비-천연 아미노산의 혼입 - Google Patents

단백질 내로의 비-천연 아미노산의 혼입 Download PDF

Info

Publication number
KR102516695B1
KR102516695B1 KR1020177014048A KR20177014048A KR102516695B1 KR 102516695 B1 KR102516695 B1 KR 102516695B1 KR 1020177014048 A KR1020177014048 A KR 1020177014048A KR 20177014048 A KR20177014048 A KR 20177014048A KR 102516695 B1 KR102516695 B1 KR 102516695B1
Authority
KR
South Korea
Prior art keywords
leu
lys
erf1
gly
glu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020177014048A
Other languages
English (en)
Korean (ko)
Other versions
KR20170070238A (ko
Inventor
제이슨 더블류. 친
울프강 에이치. 슈미드
시몬 제이. 엘사서
유진 와이. 킴
Original Assignee
유나이티드 킹덤 리서치 앤드 이노베이션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 유나이티드 킹덤 리서치 앤드 이노베이션 filed Critical 유나이티드 킹덤 리서치 앤드 이노베이션
Publication of KR20170070238A publication Critical patent/KR20170070238A/ko
Application granted granted Critical
Publication of KR102516695B1 publication Critical patent/KR102516695B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020177014048A 2014-10-27 2015-10-21 단백질 내로의 비-천연 아미노산의 혼입 Active KR102516695B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1419109.2A GB201419109D0 (en) 2014-10-27 2014-10-27 Incorporation of unnatural amino acids into proteins
GB1419109.2 2014-10-27
PCT/GB2015/053141 WO2016066995A1 (en) 2014-10-27 2015-10-21 Incorporation of unnatural amino acids into proteins

Publications (2)

Publication Number Publication Date
KR20170070238A KR20170070238A (ko) 2017-06-21
KR102516695B1 true KR102516695B1 (ko) 2023-03-30

Family

ID=52103474

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020177014048A Active KR102516695B1 (ko) 2014-10-27 2015-10-21 단백질 내로의 비-천연 아미노산의 혼입

Country Status (9)

Country Link
US (1) US10738339B2 (https=)
EP (1) EP3212799B1 (https=)
JP (2) JP6843044B2 (https=)
KR (1) KR102516695B1 (https=)
CN (1) CN107002088B (https=)
BR (1) BR112017008108A2 (https=)
CA (1) CA2965085A1 (https=)
GB (1) GB201419109D0 (https=)
WO (1) WO2016066995A1 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2014DN09922A (https=) 2012-05-18 2015-08-14 Medical Res Council
GB2528227A (en) 2014-03-14 2016-01-20 Medical Res Council Cyclopropene amino acids and methods
WO2018044143A1 (ko) * 2016-09-05 2018-03-08 한국과학기술원 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도
CN106834349B (zh) * 2017-02-28 2019-12-13 中国人民解放军军事医学科学院 一种安全性提高的病毒基因递送系统
EP3630977B1 (en) * 2017-06-02 2024-02-21 Ambrx, Inc. Methods and compositions for promoting non-natural amino acid-containing protein production
KR20210005172A (ko) * 2018-05-01 2021-01-13 암브룩스, 인코포레이티드 항체 발현을 최적화하는 방법
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
GB201817444D0 (en) 2018-10-26 2018-12-12 Res & Innovation Uk Methods and compositions
EP3696189A1 (en) * 2019-02-14 2020-08-19 European Molecular Biology Laboratory Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner
CN111718949B (zh) * 2019-03-19 2021-10-01 宁波鲲鹏生物科技有限公司 利用双质粒系统在蛋白中引入非天然氨基酸
CN111850020B (zh) * 2019-04-25 2021-05-07 苏州鲲鹏生物技术有限公司 利用质粒系统在蛋白中引入非天然氨基酸
CN111849929B (zh) * 2019-04-30 2021-05-11 苏州鲲鹏生物技术有限公司 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶
GB201906775D0 (en) 2019-05-14 2019-06-26 Res & Innovation Uk Synthetic genome
TW202128996A (zh) * 2019-10-10 2021-08-01 美商史基普研究協會 用於活體內合成非天然多肽的組合物及方法
CN117881779A (zh) 2021-05-28 2024-04-12 英国研究与创新署 微生物及其用途
CN113481239A (zh) * 2021-07-01 2021-10-08 四川大学华西医院 一种通过Rosa26位点向细胞系中引入非天然氨基酸编码体系的方法及其细胞系
WO2023282315A1 (ja) * 2021-07-07 2023-01-12 味の素株式会社 非天然アミノ酸含有タンパク質の分泌生産法
GB202110137D0 (en) 2021-07-14 2021-08-25 Res & Innovation Uk Methods for optimising protein production
CN113699124B (zh) * 2021-09-08 2022-04-12 北京大学 一种含非天然氨基酸蛋白的制备方法
WO2023196596A2 (en) * 2022-04-08 2023-10-12 Absci Corporation Trna synthetases and methods of use thereof
EP4689125A1 (en) * 2023-03-30 2026-02-11 The Chinese University of Hong Kong Unnatural amino acid in cellulo synthesis for site-specific protein modification
CN120752342A (zh) * 2023-07-13 2025-10-03 深圳华大生命科学研究院 工程生物遏制系统及方法
CN120210144A (zh) * 2023-12-25 2025-06-27 康码（上海）生物科技有限公司 一种改造的氨酰-tRNA合成酶、核酸构建物以及基因工程菌株

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120077186A1 (en) 2010-05-07 2012-03-29 Oregon Health & Science University Use of cysteine-derived suppressor trnas for non-native amino acid incorporation

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004321117B2 (en) * 2003-12-18 2010-09-02 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
EP1807515A4 (en) * 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
JP5590649B2 (ja) 2007-09-20 2014-09-17 独立行政法人理化学研究所 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法
GB2470770A (en) 2009-06-04 2010-12-08 Medical Res Council Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs
GB201201100D0 (en) 2012-01-20 2012-03-07 Medical Res Council Polypeptides and methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120077186A1 (en) 2010-05-07 2012-03-29 Oregon Health & Science University Use of cysteine-derived suppressor trnas for non-native amino acid incorporation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EMBO Reports,제3권,9호,881-886면(2002)
RNA,제16권,1902-1914면(2010)

Also Published As

Publication number Publication date
EP3212799B1 (en) 2019-04-17
EP3212799A1 (en) 2017-09-06
BR112017008108A2 (pt) 2017-12-19
CN107002088B (zh) 2022-01-11
JP2021035374A (ja) 2021-03-04
WO2016066995A1 (en) 2016-05-06
CN107002088A (zh) 2017-08-01
JP6843044B2 (ja) 2021-03-17
KR20170070238A (ko) 2017-06-21
JP2017532043A (ja) 2017-11-02
GB201419109D0 (en) 2014-12-10
US20170356023A1 (en) 2017-12-14
US10738339B2 (en) 2020-08-11
CA2965085A1 (en) 2016-05-06

Similar Documents

Publication Publication Date Title
KR102516695B1 (ko) 단백질 내로의 비-천연 아미노산의 혼입
AU2021250992B2 (en) Compositions and methods for directing proteins to specific loci in the genome
KR102888521B1 (ko) Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna
CN101553506B (zh) Lingo结合分子及其制药用途
AU2016363696B2 (en) Single variable domain T-cell receptors
KR20080031024A (ko) 폴리단백질, 프로단백질 및 단백질분해를 사용한 ｓＯＲＦ작제물 포함 다중 유전자 발현 및 방법
US20040105855A1 (en) Humanized antibodies against human 4-1BB
AU2019227700B2 (en) Techniques for generating cell-based therapeutics using recombinant T cell receptor genes
CN113631033B (zh) 与enpp1或enpp3缺乏有关的疾病的治疗
KR20230019156A (ko) 유전자 삽입을 위한 다수의 도크들이 있는 세포주
KR20240037185A (ko) 키메라 공동자극 수용체, 케모카인 수용체, 및 세포 면역치료에서의 이의 용도
CN103834692B (zh) 一种用于表达lncRNA的慢病毒载体及其应用
KR102084227B1 (ko) 인간 형질전환 연골세포주 기반 연골질환 치료제 스크리닝 시스템
CN116143896A (zh) 一种分离的多肽及其在制备肿瘤免疫治疗产品中的用途
CN112342216B (zh) 用于提高CHO细胞的表达效率的CRISPR-Cas13d系统以及重组CHO细胞
RU2840923C1 (ru) Конструкции нуклеиновых кислот для получения белков
US20020150912A1 (en) Reporter system for cell surface receptor-ligand binding
TW202216777A (zh) 四面體抗體
CN113073118B (zh) Tet-off表达调控系统在级联放大启动子活性中的用途
CN110514628A (zh) 一种快速检测O-GlcNAc修饰的生物感应器/方法及应用
EP4433592A1 (en) Compositions and methods for production of circular nucleic acid molecules
KR102721254B1 (ko) 형질전환시킨 중국햄스터난소 세포를 활용하여 시시엔5 단백질을 제조하는 방법
KR102393402B1 (ko) 세포 내 존재 단백질과 세포 외부로 분비되는 단백질을 동시 발현하는 이중발현벡터를 포함하는 암의 예방 또는 치료용 조성물
US20050003344A1 (en) AAV2 Rep protein fusions
KR100927098B1 (ko) 에피토프 태그 치환용 재조합 벡터

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R11-asn-PN2301

R19-X000 Request for party data change rejected

St.27 status event code: A-3-3-R10-R19-oth-X000

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 4