BR112017008108A2 - incorporação de aminoácidos não naturais em proteínas - Google Patents
incorporação de aminoácidos não naturais em proteínasInfo
- Publication number
- BR112017008108A2 BR112017008108A2 BR112017008108A BR112017008108A BR112017008108A2 BR 112017008108 A2 BR112017008108 A2 BR 112017008108A2 BR 112017008108 A BR112017008108 A BR 112017008108A BR 112017008108 A BR112017008108 A BR 112017008108A BR 112017008108 A2 BR112017008108 A2 BR 112017008108A2
- Authority
- BR
- Brazil
- Prior art keywords
- unnatural amino
- amino acid
- interest
- trna
- sequence
- Prior art date
Links
- 150000001413 amino acids Chemical class 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 238000010348 incorporation Methods 0.000 title abstract 3
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 4
- 101150073803 ERF1 gene Proteins 0.000 abstract 3
- 101100301666 Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) prf1 gene Proteins 0.000 abstract 3
- 101100033735 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) prf11 gene Proteins 0.000 abstract 3
- 101100033738 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) prf12 gene Proteins 0.000 abstract 3
- 150000007523 nucleic acids Chemical group 0.000 abstract 3
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 abstract 2
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108020004705 Codon Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
- C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
- C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1419109.2A GB201419109D0 (en) | 2014-10-27 | 2014-10-27 | Incorporation of unnatural amino acids into proteins |
| PCT/GB2015/053141 WO2016066995A1 (en) | 2014-10-27 | 2015-10-21 | Incorporation of unnatural amino acids into proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112017008108A2 true BR112017008108A2 (pt) | 2017-12-19 |
Family
ID=52103474
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112017008108A BR112017008108A2 (pt) | 2014-10-27 | 2015-10-21 | incorporação de aminoácidos não naturais em proteínas |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10738339B2 (https=) |
| EP (1) | EP3212799B1 (https=) |
| JP (2) | JP6843044B2 (https=) |
| KR (1) | KR102516695B1 (https=) |
| CN (1) | CN107002088B (https=) |
| BR (1) | BR112017008108A2 (https=) |
| CA (1) | CA2965085A1 (https=) |
| GB (1) | GB201419109D0 (https=) |
| WO (1) | WO2016066995A1 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN2014DN09922A (https=) | 2012-05-18 | 2015-08-14 | Medical Res Council | |
| GB2528227A (en) | 2014-03-14 | 2016-01-20 | Medical Res Council | Cyclopropene amino acids and methods |
| WO2018044143A1 (ko) * | 2016-09-05 | 2018-03-08 | 한국과학기술원 | 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도 |
| CN106834349B (zh) * | 2017-02-28 | 2019-12-13 | 中国人民解放军军事医学科学院 | 一种安全性提高的病毒基因递送系统 |
| EP3630977B1 (en) * | 2017-06-02 | 2024-02-21 | Ambrx, Inc. | Methods and compositions for promoting non-natural amino acid-containing protein production |
| KR20210005172A (ko) * | 2018-05-01 | 2021-01-13 | 암브룩스, 인코포레이티드 | 항체 발현을 최적화하는 방법 |
| CN112673109A (zh) * | 2018-09-07 | 2021-04-16 | 免疫医疗有限责任公司 | 细胞选择方法 |
| GB201817444D0 (en) | 2018-10-26 | 2018-12-12 | Res & Innovation Uk | Methods and compositions |
| EP3696189A1 (en) * | 2019-02-14 | 2020-08-19 | European Molecular Biology Laboratory | Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner |
| CN111718949B (zh) * | 2019-03-19 | 2021-10-01 | 宁波鲲鹏生物科技有限公司 | 利用双质粒系统在蛋白中引入非天然氨基酸 |
| CN111850020B (zh) * | 2019-04-25 | 2021-05-07 | 苏州鲲鹏生物技术有限公司 | 利用质粒系统在蛋白中引入非天然氨基酸 |
| CN111849929B (zh) * | 2019-04-30 | 2021-05-11 | 苏州鲲鹏生物技术有限公司 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
| GB201906775D0 (en) | 2019-05-14 | 2019-06-26 | Res & Innovation Uk | Synthetic genome |
| TW202128996A (zh) * | 2019-10-10 | 2021-08-01 | 美商史基普研究協會 | 用於活體內合成非天然多肽的組合物及方法 |
| CN117881779A (zh) | 2021-05-28 | 2024-04-12 | 英国研究与创新署 | 微生物及其用途 |
| CN113481239A (zh) * | 2021-07-01 | 2021-10-08 | 四川大学华西医院 | 一种通过Rosa26位点向细胞系中引入非天然氨基酸编码体系的方法及其细胞系 |
| WO2023282315A1 (ja) * | 2021-07-07 | 2023-01-12 | 味の素株式会社 | 非天然アミノ酸含有タンパク質の分泌生産法 |
| GB202110137D0 (en) | 2021-07-14 | 2021-08-25 | Res & Innovation Uk | Methods for optimising protein production |
| CN113699124B (zh) * | 2021-09-08 | 2022-04-12 | 北京大学 | 一种含非天然氨基酸蛋白的制备方法 |
| WO2023196596A2 (en) * | 2022-04-08 | 2023-10-12 | Absci Corporation | Trna synthetases and methods of use thereof |
| EP4689125A1 (en) * | 2023-03-30 | 2026-02-11 | The Chinese University of Hong Kong | Unnatural amino acid in cellulo synthesis for site-specific protein modification |
| CN120752342A (zh) * | 2023-07-13 | 2025-10-03 | 深圳华大生命科学研究院 | 工程生物遏制系统及方法 |
| CN120210144A (zh) * | 2023-12-25 | 2025-06-27 | 康码（上海）生物科技有限公司 | 一种改造的氨酰-tRNA合成酶、核酸构建物以及基因工程菌株 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004321117B2 (en) * | 2003-12-18 | 2010-09-02 | The Scripps Research Institute | Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells |
| EP1807515A4 (en) * | 2004-10-27 | 2008-06-18 | Scripps Research Inst | Orthogonal translation components for the in vivo incorporation of unnatural amino acids |
| JP5590649B2 (ja) | 2007-09-20 | 2014-09-17 | 独立行政法人理化学研究所 | 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 |
| GB2470770A (en) | 2009-06-04 | 2010-12-08 | Medical Res Council | Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs |
| US20120077186A1 (en) * | 2010-05-07 | 2012-03-29 | Oregon Health & Science University | Use of cysteine-derived suppressor trnas for non-native amino acid incorporation |
| GB201201100D0 (en) | 2012-01-20 | 2012-03-07 | Medical Res Council | Polypeptides and methods |
-
2014
- 2014-10-27 GB GBGB1419109.2A patent/GB201419109D0/en not_active Ceased
-
2015
- 2015-10-21 KR KR1020177014048A patent/KR102516695B1/ko active Active
- 2015-10-21 WO PCT/GB2015/053141 patent/WO2016066995A1/en not_active Ceased
- 2015-10-21 CA CA2965085A patent/CA2965085A1/en not_active Abandoned
- 2015-10-21 BR BR112017008108A patent/BR112017008108A2/pt not_active Application Discontinuation
- 2015-10-21 JP JP2017519813A patent/JP6843044B2/ja active Active
- 2015-10-21 CN CN201580058784.6A patent/CN107002088B/zh active Active
- 2015-10-21 US US15/521,103 patent/US10738339B2/en active Active
- 2015-10-21 EP EP15794622.9A patent/EP3212799B1/en active Active
-
2020
- 2020-10-22 JP JP2020177605A patent/JP2021035374A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP3212799B1 (en) | 2019-04-17 |
| EP3212799A1 (en) | 2017-09-06 |
| CN107002088B (zh) | 2022-01-11 |
| JP2021035374A (ja) | 2021-03-04 |
| WO2016066995A1 (en) | 2016-05-06 |
| CN107002088A (zh) | 2017-08-01 |
| JP6843044B2 (ja) | 2021-03-17 |
| KR20170070238A (ko) | 2017-06-21 |
| JP2017532043A (ja) | 2017-11-02 |
| GB201419109D0 (en) | 2014-12-10 |
| US20170356023A1 (en) | 2017-12-14 |
| US10738339B2 (en) | 2020-08-11 |
| KR102516695B1 (ko) | 2023-03-30 |
| CA2965085A1 (en) | 2016-05-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25A | Requested transfer of rights approved |
Owner name: UNITED KINGDOM RESEARCH AND INNOVATION (GB) |
|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |