KR102435269B1 - 미생물 세포로부터의 미생물 오일의 수득 방법 - Google Patents

미생물 세포로부터의 미생물 오일의 수득 방법 Download PDF

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Publication number
KR102435269B1
KR102435269B1 KR1020167019587A KR20167019587A KR102435269B1 KR 102435269 B1 KR102435269 B1 KR 102435269B1 KR 1020167019587 A KR1020167019587 A KR 1020167019587A KR 20167019587 A KR20167019587 A KR 20167019587A KR 102435269 B1 KR102435269 B1 KR 102435269B1
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oil
cell composition
sec
lysed cell
microbial
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Korean (ko)
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KR20160100382A (ko
Inventor
마크 바커
나스린 타바예네자드
진저 섕크
닐 프란시스 레이닝거
커트 라이벨 시니어 매튜스
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디에스엠 아이피 어셋츠 비.브이.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/48—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/487—Separation; Purification; Stabilisation; Use of additives by treatment giving rise to chemical modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C57/00—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms
    • C07C57/02—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms with only carbon-to-carbon double bonds as unsaturation
    • C07C57/03—Monocarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/06—Refining fats or fatty oils by chemical reaction with bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Polymers & Plastics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020167019587A 2013-12-20 2014-12-19 미생물 세포로부터의 미생물 오일의 수득 방법 Active KR102435269B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361919000P 2013-12-20 2013-12-20
US61/919,000 2013-12-20
PCT/US2014/071466 WO2015095693A2 (en) 2013-12-20 2014-12-19 Processes for obtaining microbial oil from microbial cells

Publications (2)

Publication Number Publication Date
KR20160100382A KR20160100382A (ko) 2016-08-23
KR102435269B1 true KR102435269B1 (ko) 2022-08-22

Family

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KR1020167019587A Active KR102435269B1 (ko) 2013-12-20 2014-12-19 미생물 세포로부터의 미생물 오일의 수득 방법

Country Status (15)

Country Link
US (1) US10364207B2 (enExample)
EP (1) EP3082794B1 (enExample)
JP (3) JP2017504318A (enExample)
KR (1) KR102435269B1 (enExample)
CN (1) CN106061475B (enExample)
AR (2) AR098891A1 (enExample)
AU (1) AU2014369045B2 (enExample)
BR (1) BR112016014516B1 (enExample)
CA (1) CA2934506C (enExample)
IL (1) IL246342B (enExample)
MX (1) MX2016008228A (enExample)
NZ (1) NZ721413A (enExample)
SG (1) SG11201605052XA (enExample)
TW (1) TWI698520B (enExample)
WO (1) WO2015095693A2 (enExample)

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AU2011261455B2 (en) 2010-06-01 2016-03-24 Dsm Ip Assets B.V. Extraction of lipid from cells and products therefrom
CN106029624B (zh) 2013-12-20 2021-12-07 帝斯曼知识产权资产管理有限公司 用于从微生物细胞获得微生物油的方法
EP3082794B1 (en) 2013-12-20 2024-05-08 DSM IP Assets B.V. Processes for obtaining microbial oil from microbial cells
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CA2934490C (en) 2013-12-20 2022-05-03 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
AU2014369042B2 (en) 2013-12-20 2020-04-30 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
US20190249108A1 (en) * 2016-07-13 2019-08-15 Stephen Robert Cherinko Method for extracting a microbial oil comprising polyunsaturated fatty acids from a fermentation broth containing oleaginous microorganisms
AU2017297752B2 (en) * 2016-07-13 2021-09-23 Dsm Ip Assets B.V. Method for isolating lipids from lipid-containing cells
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EP3562925B1 (en) 2016-12-27 2021-03-10 Evonik Operations GmbH Method of isolating lipids from a lipids containing biomass
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EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
WO2019191544A1 (en) * 2018-03-30 2019-10-03 Dsm Ip Assets B.V. Method of obtaining a microbial oil and a method of reducing emulsion by maintaining a low concentration of carbohydrate
CA3101855C (en) 2018-05-15 2023-06-20 Evonik Operations Gmbh Method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica
BR112020023222A2 (pt) 2018-05-15 2021-03-23 Evonik Operations Gmbh método de isolamento de lipídios a partir de uma biomassa contendo lipídios lisados por inversão por emulsão
CA3104845A1 (en) 2018-06-29 2020-01-02 Amyris, Inc. Methods for recovering water-immiscible isoprenoid compounds from microbial biomass
EP3696273A1 (en) * 2019-02-18 2020-08-19 Technische Universität München A method for producing microbial lipids
EP3938339A4 (en) * 2019-03-14 2022-11-16 DSM IP Assets B.V. PROCEDURE FOR RECOVERING LIPIDS FROM A MICROBIAL CELL COMPOSITION
US20220154098A1 (en) * 2019-03-14 2022-05-19 Dsm Ip Assets B.V. Methods of obtaining lipids from a microbial cell composition by enzyme and ph shock
CN113684088B (zh) * 2020-05-18 2024-06-11 嘉必优生物技术（武汉）股份有限公司 一种微生物油脂提取方法及其所得微生物油脂
EP3933016A1 (en) * 2020-06-30 2022-01-05 Evonik Operations GmbH Method of isolating lipids from a lipids containing biomass

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