KR102059866B1 - 세포-표면 항원의 면역결합제를 동정하는 방법 - Google Patents

세포-표면 항원의 면역결합제를 동정하는 방법 Download PDF

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Publication number
KR102059866B1
KR102059866B1 KR1020197005221A KR20197005221A KR102059866B1 KR 102059866 B1 KR102059866 B1 KR 102059866B1 KR 1020197005221 A KR1020197005221 A KR 1020197005221A KR 20197005221 A KR20197005221 A KR 20197005221A KR 102059866 B1 KR102059866 B1 KR 102059866B1
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South Korea
Prior art keywords
xaa xaa
cells
antigen
cell
antibody
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KR1020197005221A
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English (en)
Korean (ko)
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KR20190020202A (ko
Inventor
발레리 휼만-코티에르
다비트 우레히
Original Assignee
에스바테크 - 어 노바티스 컴파니 엘엘씨
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Priority claimed from PCT/CH2009/000222 external-priority patent/WO2009155726A2/en
Application filed by 에스바테크 - 어 노바티스 컴파니 엘엘씨 filed Critical 에스바테크 - 어 노바티스 컴파니 엘엘씨
Priority claimed from PCT/CH2010/000044 external-priority patent/WO2010096941A1/en
Publication of KR20190020202A publication Critical patent/KR20190020202A/ko
Application granted granted Critical
Publication of KR102059866B1 publication Critical patent/KR102059866B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1020197005221A 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법 KR102059866B1 (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US15510509P 2009-02-24 2009-02-24
US15504109P 2009-02-24 2009-02-24
US61/155,041 2009-02-24
US61/155,105 2009-02-24
CH832/09 2009-06-02
CH8322009 2009-06-02
PCT/CH2009/000222 WO2009155726A2 (en) 2008-06-25 2009-06-25 Humanization of rabbit antibodies using a universal antibody framework
CHPCT/CH2009/000222 2009-06-25
PCT/CH2010/000044 WO2010096941A1 (en) 2009-02-24 2010-02-22 Methods for identifying immunobinders of cell-surface antigens

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020187000320A Division KR20180005752A (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법

Publications (2)

Publication Number Publication Date
KR20190020202A KR20190020202A (ko) 2019-02-27
KR102059866B1 true KR102059866B1 (ko) 2019-12-27

Family

ID=48917567

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020197005221A KR102059866B1 (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법
KR1020167035683A KR101817279B1 (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법
KR1020167003693A KR101689791B1 (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법
KR1020187000320A KR20180005752A (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법

Family Applications After (3)

Application Number Title Priority Date Filing Date
KR1020167035683A KR101817279B1 (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법
KR1020167003693A KR101689791B1 (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법
KR1020187000320A KR20180005752A (ko) 2009-02-24 2010-02-22 세포-표면 항원의 면역결합제를 동정하는 방법

Country Status (12)

Country Link
JP (3) JP6161655B2 (zh)
KR (4) KR102059866B1 (zh)
CN (1) CN105116138A (zh)
BR (1) BRPI1005398A2 (zh)
CL (1) CL2011001453A1 (zh)
ES (1) ES2618181T3 (zh)
IL (4) IL226955A (zh)
MX (1) MX352922B (zh)
PH (1) PH12014502468A1 (zh)
RU (2) RU2577965C2 (zh)
SG (3) SG10201708233VA (zh)
ZA (1) ZA201103125B (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016462B (zh) * 2019-02-20 2020-04-24 优睿赛思(武汉)生物科技有限公司 从脾脏细胞中高效分离单个抗原特异性b淋巴细胞的方法

Citations (3)

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WO2003097697A2 (en) * 2002-05-22 2003-11-27 Esbatech Ag Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
WO2004016740A2 (en) 2002-08-15 2004-02-26 Epitomics, Inc. Humanized rabbit antibodies
US20050033031A1 (en) 2003-08-07 2005-02-10 Couto Fernando Jose Rebelo Do Methods for humanizing rabbit monoclonal antibodies

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JPS63253098A (ja) * 1987-04-10 1988-10-20 Ube Ind Ltd ヒト抗体に対するモノクロ−ナル抗体、その製法及び用途
IL91948A (en) 1988-10-26 1994-05-30 Immunomedics Removal of immunoreactive components from a sample
US5417972A (en) * 1993-08-02 1995-05-23 The Board Of Trustees Of The Leland Stanford Junior University Method of killing B-cells in a complement independent and an ADCC independent manner using antibodies which specifically bind CDIM
KR0119909B1 (ko) * 1993-10-05 1997-10-20 김은영 플로우 사이토미터를 이용한 고상형 면역 측정방법
DE69637481T2 (de) * 1995-04-27 2009-04-09 Amgen Fremont Inc. Aus immunisierten Xenomäusen stammende menschliche Antikörper gegen IL-8
ATE253593T1 (de) * 1996-07-16 2003-11-15 Plueckthun Andreas Prof Dr Immunglobulin-superfamilie domainen und fragmente mit erhöhter löslichkeit
CN1396182A (zh) * 2002-07-31 2003-02-12 陕西超英生物医学研究开发有限公司 人内皮生长因子受体2蛋白全人单克隆抗体及其制备方法
EP1561107A1 (en) * 2002-11-13 2005-08-10 Micromet AG Method for identifying antigen specific b cells
JP4336812B2 (ja) * 2003-03-07 2009-09-30 博夫 岩田 細胞に発現する表面抗原を迅速に同定するための分析方法
US20070087331A1 (en) * 2003-05-15 2007-04-19 Cytos Biotechnology Ag Selection of b cells with specificity if interest: method of preparation and use
WO2007003041A1 (en) * 2005-07-01 2007-01-11 John Schrader Methods of isolating cells and generating monoclonal antibodies
US20070134249A1 (en) 2005-12-08 2007-06-14 Genitope Corporation Combination therapy and antibody panels
CA2635849C (en) * 2005-12-28 2017-05-09 Asubio Pharma Co., Ltd. An antibody against periostin, and a pharmaceutical composition comprising it for preventing or treating a disease in which periostin is involved
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WO2008004834A1 (en) * 2006-07-06 2008-01-10 Isu Abxis Co., Ltd Humanized monoclonal antibody highly binding to epidermal growth factor receptor, egf receptor

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Publication number Priority date Publication date Assignee Title
WO2003097697A2 (en) * 2002-05-22 2003-11-27 Esbatech Ag Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
WO2004016740A2 (en) 2002-08-15 2004-02-26 Epitomics, Inc. Humanized rabbit antibodies
US20040086979A1 (en) 2002-08-15 2004-05-06 Dongxiao Zhang Humanized rabbit antibodies
US20050033031A1 (en) 2003-08-07 2005-02-10 Couto Fernando Jose Rebelo Do Methods for humanizing rabbit monoclonal antibodies
WO2005016950A1 (en) * 2003-08-07 2005-02-24 Epitomics, Inc. Methods for humanizing rabbit monoclonal antibodies

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J Immunol. 1993 Apr 1;150(7): 2844-2857

Also Published As

Publication number Publication date
JP6604989B2 (ja) 2019-11-13
KR20180005752A (ko) 2018-01-16
CN105116138A (zh) 2015-12-02
IL237985B (en) 2018-12-31
SG10201408247SA (en) 2015-02-27
KR101689791B1 (ko) 2016-12-26
JP6161655B2 (ja) 2017-07-12
KR20190020202A (ko) 2019-02-27
SG10201708233VA (en) 2017-11-29
BRPI1005398A2 (pt) 2019-03-26
CL2011001453A1 (es) 2012-02-24
ES2618181T3 (es) 2017-06-21
JP2017192384A (ja) 2017-10-26
RU2017135831A (ru) 2019-04-09
KR20160022397A (ko) 2016-02-29
IL226955A0 (en) 2013-07-31
IL226955A (en) 2015-04-30
IL263158B (en) 2019-10-31
PH12014502468B1 (en) 2016-01-18
IL263158A (en) 2018-12-31
JP2015146825A (ja) 2015-08-20
PH12014502468A1 (en) 2016-01-18
MX352922B (es) 2017-12-14
RU2017135831A3 (zh) 2019-04-09
IL226956A (en) 2015-04-30
RU2577965C2 (ru) 2016-03-20
SG2014014864A (en) 2014-09-26
IL226956A0 (en) 2013-07-31
KR20160150642A (ko) 2016-12-30
JP2018196395A (ja) 2018-12-13
KR101817279B1 (ko) 2018-01-10
RU2011124039A (ru) 2012-12-20
ZA201103125B (en) 2012-08-29

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