BRPI1005398A2 - métodos para identificação de imunoligantes de antígenos na superfície celular. - Google Patents

métodos para identificação de imunoligantes de antígenos na superfície celular.

Info

Publication number
BRPI1005398A2
BRPI1005398A2 BRPI1005398-0A BRPI1005398A BRPI1005398A2 BR PI1005398 A2 BRPI1005398 A2 BR PI1005398A2 BR PI1005398 A BRPI1005398 A BR PI1005398A BR PI1005398 A2 BRPI1005398 A2 BR PI1005398A2
Authority
BR
Brazil
Prior art keywords
methods
immunoligants
cell surface
identification
surface antigen
Prior art date
Application number
BRPI1005398-0A
Other languages
English (en)
Inventor
Hulmann-Cottier Valerie
Urech David
Original Assignee
Esbatech, An Alcon Biomedical Research Unit Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/CH2009/000222 external-priority patent/WO2009155726A2/en
Application filed by Esbatech, An Alcon Biomedical Research Unit Llc filed Critical Esbatech, An Alcon Biomedical Research Unit Llc
Priority claimed from PCT/CH2010/000044 external-priority patent/WO2010096941A1/en
Publication of BRPI1005398A2 publication Critical patent/BRPI1005398A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

métodos para identificação de imunoligantes e clones de células 8 que se ligam especificamente a um antígeno de interesse na superfície celular, para produção in vitro de tal imunoligante, o referido imunoligante, molécula de ácido nucleico codificando-o, bem como seu uso a presente invenção refere-se a métodos para a identificação de imunoligantes, tais como anticorpos scfv, capazes de se ligar especificamente a antígenos na superfície celular e composições identificadas de acordo com os referidos métodos.
BRPI1005398-0A 2009-02-24 2010-02-22 métodos para identificação de imunoligantes de antígenos na superfície celular. BRPI1005398A2 (pt)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US15510509P 2009-02-24 2009-02-24
US15504109P 2009-02-24 2009-02-24
US61/155,041 2009-02-24
US61/155,105 2009-02-24
CH832/09 2009-06-02
CH8322009 2009-06-02
PCT/CH2009/000222 WO2009155726A2 (en) 2008-06-25 2009-06-25 Humanization of rabbit antibodies using a universal antibody framework
CHPCT/CH2009/000222 2009-06-25
PCT/CH2010/000044 WO2010096941A1 (en) 2009-02-24 2010-02-22 Methods for identifying immunobinders of cell-surface antigens

Publications (1)

Publication Number Publication Date
BRPI1005398A2 true BRPI1005398A2 (pt) 2019-03-26

Family

ID=48917567

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1005398-0A BRPI1005398A2 (pt) 2009-02-24 2010-02-22 métodos para identificação de imunoligantes de antígenos na superfície celular.

Country Status (12)

Country Link
JP (3) JP6161655B2 (pt)
KR (4) KR102059866B1 (pt)
CN (1) CN105116138A (pt)
BR (1) BRPI1005398A2 (pt)
CL (1) CL2011001453A1 (pt)
ES (1) ES2618181T3 (pt)
IL (4) IL226955A (pt)
MX (1) MX352922B (pt)
PH (1) PH12014502468A1 (pt)
RU (2) RU2577965C2 (pt)
SG (3) SG10201708233VA (pt)
ZA (1) ZA201103125B (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016462B (zh) * 2019-02-20 2020-04-24 优睿赛思(武汉)生物科技有限公司 从脾脏细胞中高效分离单个抗原特异性b淋巴细胞的方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63253098A (ja) * 1987-04-10 1988-10-20 Ube Ind Ltd ヒト抗体に対するモノクロ−ナル抗体、その製法及び用途
IL91948A (en) 1988-10-26 1994-05-30 Immunomedics Removal of immunoreactive components from a sample
US5417972A (en) * 1993-08-02 1995-05-23 The Board Of Trustees Of The Leland Stanford Junior University Method of killing B-cells in a complement independent and an ADCC independent manner using antibodies which specifically bind CDIM
KR0119909B1 (ko) * 1993-10-05 1997-10-20 김은영 플로우 사이토미터를 이용한 고상형 면역 측정방법
DE69637481T2 (de) * 1995-04-27 2009-04-09 Amgen Fremont Inc. Aus immunisierten Xenomäusen stammende menschliche Antikörper gegen IL-8
ATE253593T1 (de) * 1996-07-16 2003-11-15 Plueckthun Andreas Prof Dr Immunglobulin-superfamilie domainen und fragmente mit erhöhter löslichkeit
NZ536412A (en) * 2002-05-22 2008-10-31 Esbatech Ag Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
CN1396182A (zh) * 2002-07-31 2003-02-12 陕西超英生物医学研究开发有限公司 人内皮生长因子受体2蛋白全人单克隆抗体及其制备方法
US20040086979A1 (en) 2002-08-15 2004-05-06 Dongxiao Zhang Humanized rabbit antibodies
EP1561107A1 (en) * 2002-11-13 2005-08-10 Micromet AG Method for identifying antigen specific b cells
JP4336812B2 (ja) * 2003-03-07 2009-09-30 博夫 岩田 細胞に発現する表面抗原を迅速に同定するための分析方法
US20070087331A1 (en) * 2003-05-15 2007-04-19 Cytos Biotechnology Ag Selection of b cells with specificity if interest: method of preparation and use
WO2005016950A1 (en) * 2003-08-07 2005-02-24 Epitomics, Inc. Methods for humanizing rabbit monoclonal antibodies
WO2007003041A1 (en) * 2005-07-01 2007-01-11 John Schrader Methods of isolating cells and generating monoclonal antibodies
US20070134249A1 (en) 2005-12-08 2007-06-14 Genitope Corporation Combination therapy and antibody panels
CA2635849C (en) * 2005-12-28 2017-05-09 Asubio Pharma Co., Ltd. An antibody against periostin, and a pharmaceutical composition comprising it for preventing or treating a disease in which periostin is involved
MY159787A (en) * 2006-06-02 2017-01-31 Regeneron Pharma High affinity antibodies to human il-6 receptor
WO2008004834A1 (en) * 2006-07-06 2008-01-10 Isu Abxis Co., Ltd Humanized monoclonal antibody highly binding to epidermal growth factor receptor, egf receptor

Also Published As

Publication number Publication date
JP6604989B2 (ja) 2019-11-13
KR20180005752A (ko) 2018-01-16
CN105116138A (zh) 2015-12-02
IL237985B (en) 2018-12-31
SG10201408247SA (en) 2015-02-27
KR101689791B1 (ko) 2016-12-26
JP6161655B2 (ja) 2017-07-12
KR20190020202A (ko) 2019-02-27
SG10201708233VA (en) 2017-11-29
CL2011001453A1 (es) 2012-02-24
ES2618181T3 (es) 2017-06-21
JP2017192384A (ja) 2017-10-26
RU2017135831A (ru) 2019-04-09
KR20160022397A (ko) 2016-02-29
IL226955A0 (en) 2013-07-31
IL226955A (en) 2015-04-30
IL263158B (en) 2019-10-31
PH12014502468B1 (en) 2016-01-18
IL263158A (en) 2018-12-31
JP2015146825A (ja) 2015-08-20
PH12014502468A1 (en) 2016-01-18
MX352922B (es) 2017-12-14
RU2017135831A3 (pt) 2019-04-09
IL226956A (en) 2015-04-30
RU2577965C2 (ru) 2016-03-20
SG2014014864A (en) 2014-09-26
IL226956A0 (en) 2013-07-31
KR20160150642A (ko) 2016-12-30
JP2018196395A (ja) 2018-12-13
KR101817279B1 (ko) 2018-01-10
RU2011124039A (ru) 2012-12-20
ZA201103125B (en) 2012-08-29
KR102059866B1 (ko) 2019-12-27

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]