IL226955A - Methods for identifying b-cell clones that bind specifically to a desired antigen, a method of training binding and training binders produced by these methods - Google Patents

Methods for identifying b-cell clones that bind specifically to a desired antigen, a method of training binding and training binders produced by these methods

Info

Publication number
IL226955A
IL226955A IL226955A IL22695513A IL226955A IL 226955 A IL226955 A IL 226955A IL 226955 A IL226955 A IL 226955A IL 22695513 A IL22695513 A IL 22695513A IL 226955 A IL226955 A IL 226955A
Authority
IL
Israel
Prior art keywords
methods
immunobinders
immunobinder
antigen
interest
Prior art date
Application number
IL226955A
Other languages
English (en)
Hebrew (he)
Other versions
IL226955A0 (en
Original Assignee
Esbatech Alcon Biomed Res Unit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/CH2009/000222 external-priority patent/WO2009155726A2/en
Priority claimed from PCT/CH2010/000044 external-priority patent/WO2010096941A1/en
Application filed by Esbatech Alcon Biomed Res Unit filed Critical Esbatech Alcon Biomed Res Unit
Publication of IL226955A0 publication Critical patent/IL226955A0/en
Publication of IL226955A publication Critical patent/IL226955A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
IL226955A 2009-02-24 2013-06-13 Methods for identifying b-cell clones that bind specifically to a desired antigen, a method of training binding and training binders produced by these methods IL226955A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US15504109P 2009-02-24 2009-02-24
US15510509P 2009-02-24 2009-02-24
CH8322009 2009-06-02
PCT/CH2009/000222 WO2009155726A2 (en) 2008-06-25 2009-06-25 Humanization of rabbit antibodies using a universal antibody framework
PCT/CH2010/000044 WO2010096941A1 (en) 2009-02-24 2010-02-22 Methods for identifying immunobinders of cell-surface antigens

Publications (2)

Publication Number Publication Date
IL226955A0 IL226955A0 (en) 2013-07-31
IL226955A true IL226955A (en) 2015-04-30

Family

ID=48917567

Family Applications (4)

Application Number Title Priority Date Filing Date
IL226956A IL226956A (en) 2009-02-24 2013-06-13 Methods for identifying training binds that bind specifically to a desired antigen
IL226955A IL226955A (en) 2009-02-24 2013-06-13 Methods for identifying b-cell clones that bind specifically to a desired antigen, a method of training binding and training binders produced by these methods
IL237985A IL237985B (en) 2009-02-24 2015-03-26 A humanized immunolinker capable of binding to a desired antigen
IL26315818A IL263158B (en) 2009-02-24 2018-11-20 Methods for the detection of immunobinding antigens on the surface of cells

Family Applications Before (1)

Application Number Title Priority Date Filing Date
IL226956A IL226956A (en) 2009-02-24 2013-06-13 Methods for identifying training binds that bind specifically to a desired antigen

Family Applications After (2)

Application Number Title Priority Date Filing Date
IL237985A IL237985B (en) 2009-02-24 2015-03-26 A humanized immunolinker capable of binding to a desired antigen
IL26315818A IL263158B (en) 2009-02-24 2018-11-20 Methods for the detection of immunobinding antigens on the surface of cells

Country Status (12)

Country Link
JP (3) JP6161655B2 (zh)
KR (4) KR102059866B1 (zh)
CN (1) CN105116138A (zh)
BR (1) BRPI1005398A2 (zh)
CL (1) CL2011001453A1 (zh)
ES (1) ES2618181T3 (zh)
IL (4) IL226956A (zh)
MX (1) MX352922B (zh)
PH (1) PH12014502468A1 (zh)
RU (2) RU2577965C2 (zh)
SG (3) SG10201708233VA (zh)
ZA (1) ZA201103125B (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016462B (zh) * 2019-02-20 2020-04-24 优睿赛思(武汉)生物科技有限公司 从脾脏细胞中高效分离单个抗原特异性b淋巴细胞的方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63253098A (ja) * 1987-04-10 1988-10-20 Ube Ind Ltd ヒト抗体に対するモノクロ−ナル抗体、その製法及び用途
IL91948A (en) * 1988-10-26 1994-05-30 Immunomedics Removal of immunoreactive components from a sample
US5417972A (en) * 1993-08-02 1995-05-23 The Board Of Trustees Of The Leland Stanford Junior University Method of killing B-cells in a complement independent and an ADCC independent manner using antibodies which specifically bind CDIM
KR0119909B1 (ko) * 1993-10-05 1997-10-20 김은영 플로우 사이토미터를 이용한 고상형 면역 측정방법
KR20050085971A (ko) * 1995-04-27 2005-08-29 아브게닉스, 인크. 면역화된 제노마우스 유래의 인간 항체
WO1998002462A1 (en) * 1996-07-16 1998-01-22 Morphosys Gesellschaft Für Proteinoptimierung Mbh Immunoglobulin superfamily domains and fragments with increased solubility
WO2003097697A2 (en) * 2002-05-22 2003-11-27 Esbatech Ag Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
CN1396182A (zh) * 2002-07-31 2003-02-12 陕西超英生物医学研究开发有限公司 人内皮生长因子受体2蛋白全人单克隆抗体及其制备方法
WO2004016740A2 (en) * 2002-08-15 2004-02-26 Epitomics, Inc. Humanized rabbit antibodies
CA2505924A1 (en) * 2002-11-13 2004-05-27 Micromet Ag Method for identifying antigen specific b cells
JP4336812B2 (ja) * 2003-03-07 2009-09-30 博夫 岩田 細胞に発現する表面抗原を迅速に同定するための分析方法
EP1623229A2 (en) * 2003-05-15 2006-02-08 Cytos Biotechnology AG Selection of b cells with specificity of interest: method of preparation and use
EP1651659A4 (en) * 2003-08-07 2008-09-17 Epitomics Inc METHOD FOR HUMANIZATION OF MONOCLONAL RABBIT ANTIBODIES
CA2655511C (en) * 2005-07-01 2017-03-21 John Schrader Methods of isolating cells and generating monoclonal antibodies
US20070134249A1 (en) 2005-12-08 2007-06-14 Genitope Corporation Combination therapy and antibody panels
RU2008130898A (ru) * 2005-12-28 2010-02-10 Асубио Фарма Ко., Лтд. (Jp) Антитело против периостина и содержащая его фармацевтическая композиция для профилактики или лечения заболевания, в развитии которого участвует периостин
CN102585002A (zh) * 2006-06-02 2012-07-18 瑞泽恩制药公司 人il-6受体的高亲和力抗体
WO2008004834A1 (en) * 2006-07-06 2008-01-10 Isu Abxis Co., Ltd Humanized monoclonal antibody highly binding to epidermal growth factor receptor, egf receptor

Also Published As

Publication number Publication date
ZA201103125B (en) 2012-08-29
RU2017135831A3 (zh) 2019-04-09
KR20180005752A (ko) 2018-01-16
IL226955A0 (en) 2013-07-31
SG10201408247SA (en) 2015-02-27
PH12014502468B1 (en) 2016-01-18
KR102059866B1 (ko) 2019-12-27
KR101817279B1 (ko) 2018-01-10
SG10201708233VA (en) 2017-11-29
JP2015146825A (ja) 2015-08-20
RU2577965C2 (ru) 2016-03-20
SG2014014864A (en) 2014-09-26
MX352922B (es) 2017-12-14
KR20160150642A (ko) 2016-12-30
RU2011124039A (ru) 2012-12-20
IL263158A (en) 2018-12-31
JP2017192384A (ja) 2017-10-26
JP2018196395A (ja) 2018-12-13
KR101689791B1 (ko) 2016-12-26
JP6604989B2 (ja) 2019-11-13
KR20160022397A (ko) 2016-02-29
PH12014502468A1 (en) 2016-01-18
CL2011001453A1 (es) 2012-02-24
JP6161655B2 (ja) 2017-07-12
CN105116138A (zh) 2015-12-02
IL263158B (en) 2019-10-31
RU2017135831A (ru) 2019-04-09
ES2618181T3 (es) 2017-06-21
IL226956A (en) 2015-04-30
KR20190020202A (ko) 2019-02-27
BRPI1005398A2 (pt) 2019-03-26
IL226956A0 (en) 2013-07-31
IL237985B (en) 2018-12-31

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