KR101963920B1 - Nk 세포의 증폭 방법 - Google Patents

Nk 세포의 증폭 방법 Download PDF

Info

Publication number
KR101963920B1
KR101963920B1 KR1020147001579A KR20147001579A KR101963920B1 KR 101963920 B1 KR101963920 B1 KR 101963920B1 KR 1020147001579 A KR1020147001579 A KR 1020147001579A KR 20147001579 A KR20147001579 A KR 20147001579A KR 101963920 B1 KR101963920 B1 KR 101963920B1
Authority
KR
South Korea
Prior art keywords
cells
cell
positive
amplification
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020147001579A
Other languages
English (en)
Korean (ko)
Other versions
KR20140051263A (ko
Inventor
요시카즈 요네미츠
유이 하라다
사토루 사이토
유이치로 야자키
마사토 오카모토
다케후미 이시다오
Original Assignee
고쿠리쓰다이가쿠호진 규슈다이가쿠
가부시키가이샤 가이아바이오메디신
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 고쿠리쓰다이가쿠호진 규슈다이가쿠, 가부시키가이샤 가이아바이오메디신 filed Critical 고쿠리쓰다이가쿠호진 규슈다이가쿠
Publication of KR20140051263A publication Critical patent/KR20140051263A/ko
Application granted granted Critical
Publication of KR101963920B1 publication Critical patent/KR101963920B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0646Natural killers cells [NK], NKT cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14Blood; Artificial blood
    • A61K35/17Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00Cellular immunotherapy
    • A61K40/10Cellular immunotherapy characterised by the cell type used
    • A61K40/15Natural-killer [NK] cells; Natural-killer T [NKT] cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00Cellular immunotherapy
    • A61K40/40Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41Vertebrate antigens
    • A61K40/42Cancer antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081Purging biological preparations of unwanted cells
    • C12N5/0087Purging against subsets of blood cells, e.g. purging alloreactive T cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/2302Interleukin-2 (IL-2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020147001579A 2011-06-24 2012-06-20 Nk 세포의 증폭 방법 Active KR101963920B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JPJP-P-2011-140725 2011-06-24
JP2011140725 2011-06-24
JPJP-P-2012-021972 2012-02-03
JP2012021972A JP5572863B2 (ja) 2011-06-24 2012-02-03 Nk細胞の増幅方法
PCT/JP2012/065718 WO2012176796A1 (ja) 2011-06-24 2012-06-20 Nk細胞の増幅方法

Publications (2)

Publication Number Publication Date
KR20140051263A KR20140051263A (ko) 2014-04-30
KR101963920B1 true KR101963920B1 (ko) 2019-03-29

Family

ID=47422628

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147001579A Active KR101963920B1 (ko) 2011-06-24 2012-06-20 Nk 세포의 증폭 방법

Country Status (9)

Country Link
US (1) US9404083B2 (enExample)
EP (1) EP2725100B1 (enExample)
JP (1) JP5572863B2 (enExample)
KR (1) KR101963920B1 (enExample)
CN (1) CN103620022B (enExample)
AU (1) AU2012274478B2 (enExample)
CA (1) CA2840161C (enExample)
MY (1) MY161389A (enExample)
WO (1) WO2012176796A1 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5511039B1 (ja) * 2013-05-22 2014-06-04 国立大学法人九州大学 Nk細胞の調製方法
JP6164650B2 (ja) * 2014-01-20 2017-07-19 国立大学法人九州大学 Nk細胞の調製方法
WO2015132415A1 (en) * 2014-03-07 2015-09-11 Emercell Sas Pooled nk cells from ombilical cord blood and their uses for the treatment of cancer and chronic infectious disease
KR101697473B1 (ko) 2014-11-26 2017-01-18 주식회사 녹십자랩셀 T 세포를 이용한 자연살해세포의 배양방법
US12398371B2 (en) 2015-01-27 2025-08-26 Korea Research Institute Of Bioscience Method for mass producing natural killer cell and use of natural killer cell obtained by the method as anti-cancer agent
WO2016122014A1 (ko) * 2015-01-27 2016-08-04 한국생명공학연구원 자연살해세포의 대량생산 방법 및 상기 방법으로 수득된 자연살해세포의 항암제로서의 용도
CN106222141B (zh) * 2016-10-17 2018-10-19 湖南丰晖生物科技有限公司 Nk细胞培养液和细胞培养方法
JP6647240B2 (ja) 2017-05-12 2020-02-14 米満 吉和 高活性nk細胞、およびその利用
CN110662834B (zh) 2017-05-26 2023-09-12 Gc细胞治疗 使用转化的t细胞培养自然杀伤细胞的方法
KR102765799B1 (ko) * 2018-02-01 2025-02-13 주식회사 엔케이맥스 Cd56+ 자연살해세포를 포함하는 항암용 조성물
EP3746095A4 (en) 2018-02-01 2021-04-21 Nkmax Co., Ltd. Method of producing natural killer cells and composition for treating cancer
WO2019152663A1 (en) * 2018-02-01 2019-08-08 Nkmax Co., Ltd. Method of producing natural killer cells and composition for treating cancer
JP6543375B1 (ja) 2018-03-27 2019-07-10 株式会社ガイアバイオメディシン ケモカインレセプターと細胞接着分子を発現するcd3陰性細胞の集団、およびその利用
CN110628714B (zh) * 2018-06-21 2023-03-28 精准生技股份有限公司 用于体外扩增自然杀手细胞及自然杀手t细胞的无血清细胞培养液
CN109161527A (zh) * 2018-09-25 2019-01-08 深圳市五零生命科技有限公司 一种高效的nk细胞扩增方法
EP3892721A4 (en) * 2018-11-14 2022-08-31 GC Cell Corporation METHOD FOR CULTIVATING NATURAL KILLER CELLS DERIVED FROM UMBILICAL CORD BLOOD USING TRANSFORMED T LYMPHOCYTES
WO2020152661A1 (ja) 2019-01-21 2020-07-30 株式会社ガイアバイオメディシン Nk細胞を含む細胞集団の製造方法
JP6838750B2 (ja) 2019-01-21 2021-03-03 株式会社ガイアバイオメディシン Nk細胞を含む細胞集団の製造方法
KR102234394B1 (ko) * 2019-03-08 2021-03-31 신지섭 타가면역세포배양방법, 그 방법으로 얻어진 면역세포배양액 및 이를 포함하는 면역세포치료제
KR102216710B1 (ko) * 2019-03-27 2021-02-17 신지섭 Nk세포배양배지용 첨가조성물, 상기 첨가조성물을 이용한 nk세포배양방법 및 상기 배양방법으로 얻어진 피부트러블개선용 화장료조성물
JP6697611B2 (ja) * 2019-05-20 2020-05-20 米満 吉和 高活性nk細胞、およびその利用
CN111154721B (zh) * 2020-01-14 2023-10-17 深圳格泰赛尔生物科技有限公司 Nk细胞扩增方法
US20230040477A1 (en) * 2020-01-19 2023-02-09 Board Of Regents, The University Of Texas System T-cell death associated gene 8 (tdag8) modulation to enhance cellular cancer therapies
US12486492B2 (en) 2020-03-05 2025-12-02 Korea Research Institute Of Bioscience Method for producing memory-like NK cells with ability to express higher levels of NCRs, cytotoxicity, and IFN-γ than NK cells in human peripheral blood
EP4263600A1 (en) 2020-12-18 2023-10-25 Century Therapeutics, Inc. Chimeric antigen receptor systems with adaptable receptor specificity
WO2023081813A1 (en) 2021-11-05 2023-05-11 St. Jude Children's Research Hospital, Inc. Zip cytokine receptors
EP4536687A1 (en) 2022-06-08 2025-04-16 St. Jude Children's Research Hospital, Inc. Disruption of kdm4a in t cells to enhance immunotherapy
WO2024059787A1 (en) 2022-09-16 2024-03-21 St. Jude Children's Research Hospital, Inc. Disruption of asxl1 in t cells to enhance immunotherapy
WO2025022372A1 (ja) * 2023-07-21 2025-01-30 株式会社ガイアバイオメディシン 細胞集団の製造方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030068306A1 (en) * 2001-09-14 2003-04-10 Dilber Mehmet Sirac Medium
JP2006514024A (ja) 2002-12-23 2006-04-27 イネイト・ファーマ Nk細胞の増殖に対する効果を有する医薬組成物及びそれを使用する方法
WO2010110734A1 (en) 2009-03-26 2010-09-30 Avaris Ab Expansion of nk cells
JP2010220479A (ja) 2007-06-15 2010-10-07 Medeinetto:Kk Nk細胞の培養方法及びnk細胞の利用
KR101039843B1 (ko) * 2010-08-30 2011-06-09 주식회사 엔케이바이오 자기활성화 림프구 배양용 배지 조성물 및 이를 이용한 자기활성화 림프구 배양방법

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7544355B2 (en) * 2002-03-13 2009-06-09 Universita Degli Studi Di Perugia Methods and compositions for allogeneic transplantation
US7435596B2 (en) * 2004-11-04 2008-10-14 St. Jude Children's Research Hospital, Inc. Modified cell line and method for expansion of NK cell
WO2006050270A2 (en) * 2004-11-02 2006-05-11 The Government Of The United States Of America As Represented By The Secretary Department Of Health & Human Services Compositions and methods for treating hyperproliferative disorders
CA2645042A1 (en) 2006-03-06 2007-09-13 Government Of The United States Of America, Represented By The Secretary , Department Of Health And Human Services Autologous natural killer cells and lymphodepleting chemotherapy for the treatment of cancer
JP2007297292A (ja) 2006-04-28 2007-11-15 Kyorin Pharmaceut Co Ltd 1−アルコキシイソキノリン誘導体又はその水和物の製造方法
JP4275680B2 (ja) 2006-04-28 2009-06-10 裕 照沼 リンパ球の活性・増殖に係る培養方法
WO2008023874A1 (en) * 2006-08-23 2008-02-28 Binex Co., Ltd. Manufacturing method of activated lymphocytes for immunotherapy
JP4861420B2 (ja) 2006-08-28 2012-01-25 久光製薬株式会社 爪用貼付剤
AU2008307633C1 (en) * 2007-09-28 2015-04-30 Celularity Inc. Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells
DK2279253T3 (en) * 2008-04-09 2017-02-13 Maxcyte Inc Construction and application of therapeutic compositions of freshly isolated cells
KR101133185B1 (ko) * 2008-07-29 2012-04-06 서울대학교병원 자연살해세포의 증식방법
CN102597223B (zh) * 2009-09-11 2017-05-10 宝生物工程株式会社 生产天然杀伤细胞的方法
EP2519239B1 (en) 2009-12-29 2017-03-08 Gamida Cell Ltd. Methods for enhancing natural killer cell proliferation and activity
JP2011239701A (ja) 2010-05-14 2011-12-01 Tella Inc 樹状細胞の培養方法
JP5840876B2 (ja) 2011-06-24 2016-01-06 テラ株式会社 Nk細胞を増幅するための組成物及び方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030068306A1 (en) * 2001-09-14 2003-04-10 Dilber Mehmet Sirac Medium
JP2006514024A (ja) 2002-12-23 2006-04-27 イネイト・ファーマ Nk細胞の増殖に対する効果を有する医薬組成物及びそれを使用する方法
JP2010220479A (ja) 2007-06-15 2010-10-07 Medeinetto:Kk Nk細胞の培養方法及びnk細胞の利用
WO2010110734A1 (en) 2009-03-26 2010-09-30 Avaris Ab Expansion of nk cells
KR101039843B1 (ko) * 2010-08-30 2011-06-09 주식회사 엔케이바이오 자기활성화 림프구 배양용 배지 조성물 및 이를 이용한 자기활성화 림프구 배양방법

Also Published As

Publication number Publication date
JP2013027385A (ja) 2013-02-07
JP5572863B2 (ja) 2014-08-20
EP2725100A4 (en) 2015-01-07
US20140120072A1 (en) 2014-05-01
MY161389A (en) 2017-04-14
EP2725100A1 (en) 2014-04-30
US9404083B2 (en) 2016-08-02
EP2725100B1 (en) 2019-09-04
AU2012274478A1 (en) 2014-02-20
CN103620022B (zh) 2015-09-30
KR20140051263A (ko) 2014-04-30
CN103620022A (zh) 2014-03-05
CA2840161A1 (en) 2012-12-27
AU2012274478B2 (en) 2017-03-30
AU2012274478A8 (en) 2016-07-28
WO2012176796A1 (ja) 2012-12-27
HK1190432A1 (en) 2014-07-04
CA2840161C (en) 2019-02-12

Similar Documents

Publication Publication Date Title
KR101963920B1 (ko) Nk 세포의 증폭 방법
AU2014269813B2 (en) Method for preparing NK cells
US12329817B2 (en) Highly active NK cell and use thereof
JP5840876B2 (ja) Nk細胞を増幅するための組成物及び方法
JP5989016B2 (ja) Nk細胞の増幅方法
TWI757709B (zh) 含有nk細胞之細胞集團之製造方法
JP6164650B2 (ja) Nk細胞の調製方法
JP6697611B2 (ja) 高活性nk細胞、およびその利用
HK1190432B (en) Method for amplifying nk cells
JP2023153286A (ja) Nk細胞を含む細胞集団の製造方法
KR100999905B1 (ko) 생착능이 증가된 조혈줄기세포의 제조방법
EA043082B1 (ru) Способы получения tcr гамма дельта+ т-клеток

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20140121

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
N231 Notification of change of applicant
PN2301 Change of applicant

Patent event date: 20170201

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20170524

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20180716

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20190122

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20190325

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20190325

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20220302

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20250217

Start annual number: 7

End annual number: 7