KR101602972B1 - 단백질 발현 시스템 - Google Patents

단백질 발현 시스템 Download PDF

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Publication number
KR101602972B1
KR101602972B1 KR1020107017609A KR20107017609A KR101602972B1 KR 101602972 B1 KR101602972 B1 KR 101602972B1 KR 1020107017609 A KR1020107017609 A KR 1020107017609A KR 20107017609 A KR20107017609 A KR 20107017609A KR 101602972 B1 KR101602972 B1 KR 101602972B1
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South Korea
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rna
sequence
initiation site
cpmv
gene
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Korean (ko)
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KR20100109950A (ko
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프랭크 세인스버리
조지 피터 로모노쇼프
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플랜트 바이오사이언스 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
KR1020107017609A 2008-01-08 2009-01-08 단백질 발현 시스템 Expired - Fee Related KR101602972B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0800272.7 2008-01-08
GBGB0800272.7A GB0800272D0 (en) 2008-01-08 2008-01-08 Protein expression systems

Publications (2)

Publication Number Publication Date
KR20100109950A KR20100109950A (ko) 2010-10-11
KR101602972B1 true KR101602972B1 (ko) 2016-03-11

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KR1020107017609A Expired - Fee Related KR101602972B1 (ko) 2008-01-08 2009-01-08 단백질 발현 시스템

Country Status (18)

Country Link
US (5) US8674084B2 (enExample)
EP (1) EP2240589B1 (enExample)
JP (1) JP5745857B2 (enExample)
KR (1) KR101602972B1 (enExample)
CN (1) CN101952446B (enExample)
AU (1) AU2009203608B2 (enExample)
BR (1) BRPI0906509A2 (enExample)
CA (1) CA2711895C (enExample)
DK (1) DK2240589T3 (enExample)
ES (1) ES2537198T3 (enExample)
GB (1) GB0800272D0 (enExample)
IL (1) IL206606A (enExample)
MX (1) MX2010007336A (enExample)
NZ (1) NZ586939A (enExample)
PT (1) PT2240589E (enExample)
RU (1) RU2539793C2 (enExample)
WO (1) WO2009087391A1 (enExample)
ZA (1) ZA201005245B (enExample)

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CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EP2238253B1 (en) 2007-11-27 2012-09-12 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
GB0800272D0 (en) 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
CA2730171C (en) 2008-07-08 2016-10-11 Medicago Inc. Soluble recombinant influenza antigens
NZ597401A (en) 2009-06-24 2013-09-27 Medicago Inc Chimeric influenza virus-like particles comprising hemagglutinin
GB201002434D0 (en) * 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source
US20130295609A1 (en) * 2010-11-04 2013-11-07 Medicago Inc. Plant expression system
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
EP2665818B1 (en) 2011-01-17 2017-03-22 Philip Morris Products S.a.s. Protein expression in plants
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
CN116218900A (zh) 2011-09-30 2023-06-06 麦迪卡格公司 增加植物中病毒样颗粒的产率
CA2855252C (en) * 2011-11-11 2023-03-07 Philip Morris Products S.A. Influenza virus-like particles (vlps) comprising hemagglutinin produced in nicotiana tabacum
MX364778B (es) * 2012-09-05 2019-05-06 Medicago Inc Produccion de particulas similares a picornavirus en plantas.
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
ES2803508T3 (es) 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
EP3092309B1 (en) * 2014-01-10 2021-02-24 Medicago Inc. Cpmv enhancer elements
ES2912291T3 (es) 2014-03-27 2022-05-25 Medicago Inc Elementos potenciadores de CPMV modificados
US11959088B2 (en) 2014-07-11 2024-04-16 Aramis Biotechnologies Inc. Modifying protein production in plants
EA201791683A1 (ru) 2015-01-23 2017-12-29 Медикаго Инк. Получение ротавирусоподобных частиц в растениях
JP2018524345A (ja) 2015-07-02 2018-08-30 メディカゴ インコーポレイテッド ジャスモン酸経路アクチベーター
CA3052286A1 (en) * 2017-02-02 2018-08-09 Duke University Compositions and methods for controlling gene expression
GB201721600D0 (en) 2017-12-21 2018-02-07 Plant Bioscience Ltd Metabolic engineering
CN112400021A (zh) 2018-03-14 2021-02-23 麦迪卡格公司 植物表达增强子
GB201808617D0 (en) 2018-05-25 2018-07-11 Plant Bioscience Ltd Scaffold modification
WO2020033416A1 (en) * 2018-08-06 2020-02-13 Northwestern University Combined transcription and translation platform derived from plant chloroplasts
EP3938511A4 (en) * 2019-03-14 2023-02-08 Mitsubishi Tanabe Pharma Corporation Endogenous plant expression enhancer
GB201908431D0 (en) 2019-06-12 2019-07-24 Plant Bioscience Ltd Biosynthetic genes and polypeptides
WO2020256099A1 (ja) * 2019-06-21 2020-12-24 国立大学法人大阪大学 外来遺伝子を安定的に保持する人工組換えｒｎａウイルスの作製方法
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
CN110468129B (zh) * 2019-08-08 2023-03-24 四川农业大学 一种基于cmv rna2的体外干扰黄瓜花叶病的制剂、其制备方法与应用
CN111777670B (zh) * 2020-06-19 2021-07-30 东北农业大学 一种pH调节自组装抗菌肽及制备方法和应用
CN117479831A (zh) 2021-06-09 2024-01-30 南特知识产权控股有限责任公司 用于在植物中生产感兴趣的蛋白质的方法和系统
CN114181969B (zh) * 2021-12-09 2024-05-28 苏州大学 一种制备包裹外源蛋白的多角体的方法
GB202209501D0 (en) 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
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KR20250082120A (ko) * 2023-11-29 2025-06-09 서강대학교산학협력단 검은눈콩 모자이크 바이러스의 바이러스 유사 입자의 제조방법 및 이의 활용

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ES2912291T3 (es) 2014-03-27 2022-05-25 Medicago Inc Elementos potenciadores de CPMV modificados

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Also Published As

Publication number Publication date
NZ586939A (en) 2012-08-31
US20210155947A1 (en) 2021-05-27
CA2711895C (en) 2016-08-09
BRPI0906509A2 (pt) 2015-12-01
US10947549B2 (en) 2021-03-16
WO2009087391A1 (en) 2009-07-16
GB0800272D0 (en) 2008-02-13
PT2240589E (pt) 2015-06-18
US20140250549A1 (en) 2014-09-04
AU2009203608A1 (en) 2009-07-16
RU2539793C2 (ru) 2015-01-27
RU2010133217A (ru) 2012-02-20
JP2011509081A (ja) 2011-03-24
AU2009203608B2 (en) 2014-03-27
ZA201005245B (en) 2012-01-25
DK2240589T3 (en) 2015-05-04
IL206606A0 (en) 2010-12-30
US20180030462A1 (en) 2018-02-01
US10822613B2 (en) 2020-11-03
US20100287670A1 (en) 2010-11-11
CN101952446A (zh) 2011-01-19
IL206606A (en) 2015-11-30
US8674084B2 (en) 2014-03-18
EP2240589A1 (en) 2010-10-20
ES2537198T3 (es) 2015-06-03
JP5745857B2 (ja) 2015-07-08
US20200392522A1 (en) 2020-12-17
CN101952446B (zh) 2014-06-11
MX2010007336A (es) 2010-08-16
CA2711895A1 (en) 2009-07-16
EP2240589B1 (en) 2015-03-04
KR20100109950A (ko) 2010-10-11

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