EA201791683A1 - Получение ротавирусоподобных частиц в растениях - Google Patents

Получение ротавирусоподобных частиц в растениях

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Publication number
EA201791683A1
EA201791683A1 EA201791683A EA201791683A EA201791683A1 EA 201791683 A1 EA201791683 A1 EA 201791683A1 EA 201791683 A EA201791683 A EA 201791683A EA 201791683 A EA201791683 A EA 201791683A EA 201791683 A1 EA201791683 A1 EA 201791683A1
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EA
Eurasian Patent Office
Prior art keywords
host
nucleotide sequence
rlp
plant
rotavirus
Prior art date
Application number
EA201791683A
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English (en)
Inventor
Пьер-Оливье Лавуа
Марк-Андре Д'Ау
Original Assignee
Медикаго Инк.
Мицубиси Танабе Фарма Корпорейшн
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Application filed by Медикаго Инк., Мицубиси Танабе Фарма Корпорейшн filed Critical Медикаго Инк.
Publication of EA201791683A1 publication Critical patent/EA201791683A1/ru

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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N2720/12011—Reoviridae
    • C12N2720/12311—Rotavirus, e.g. rotavirus A
    • C12N2720/12351—Methods of production or purification of viral material

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  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
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  • Plant Pathology (AREA)
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  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Предусмотрен способ получения ротавирусоподобной частицы (RLP) в растении. Способ предусматривает экспрессию в хозяине или клетке-хозяине, например растении, части растения или растительной клетке, одной или более нуклеиновых кислот, содержащих одну или более регуляторных областей, функционально связанных с первой, второй и третьей нуклеотидной последовательностью, причем регуляторная область активна в хозяине или клетке-хозяине. Первая нуклеотидная последовательность кодирует первый ротавирусный белок, вторая нуклеотидная последовательность кодирует второй ротавирусный белок и третья нуклеотидная последовательность кодирует третий ротавирусный белок. Первая, вторая и третья нуклеиновые кислоты кодируют ротавирусные белки NSP4 и VP2 или VP6 и VP4 или VP7. Хозяина или клетку-хозяина инкубируют в условиях, которые позволяют экспрессию нуклеиновых кислот, так что экспрессируются NSP4 и VP2 или VP6 и VP4 или VP7, тем самым производя RLP. Также предусмотрены хозяева, содержащие RLP, композиции, содержащие RLP, и способ применения композиции.
EA201791683A 2015-01-23 2016-01-21 Получение ротавирусоподобных частиц в растениях EA201791683A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562106941P 2015-01-23 2015-01-23
PCT/CA2016/050043 WO2016115630A1 (en) 2015-01-23 2016-01-21 Rotavirus-like particle production in plants

Publications (1)

Publication Number Publication Date
EA201791683A1 true EA201791683A1 (ru) 2017-12-29

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EA201791683A EA201791683A1 (ru) 2015-01-23 2016-01-21 Получение ротавирусоподобных частиц в растениях

Country Status (17)

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US (2) US10287555B2 (ru)
EP (1) EP3256575B1 (ru)
JP (1) JP6841762B2 (ru)
KR (1) KR20170104595A (ru)
CN (1) CN107208072A (ru)
AU (1) AU2016208999A1 (ru)
BR (1) BR112017014160A2 (ru)
CA (1) CA2974438A1 (ru)
EA (1) EA201791683A1 (ru)
ES (1) ES2826402T3 (ru)
HK (1) HK1245333A1 (ru)
IL (1) IL253040A0 (ru)
MX (1) MX2017009568A (ru)
PH (1) PH12017501330A1 (ru)
SG (1) SG11201705991XA (ru)
WO (1) WO2016115630A1 (ru)
ZA (1) ZA201705555B (ru)

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US20230203101A1 (en) * 2020-04-22 2023-06-29 POSTECH Research and Business Development Foundation Coronavirus Disease 2019(COVID -19) Recombinant Spike Protein Forming Trimer, Method for Mass Producing Recombinant Spike Protein in Plants, and Method for Preparing Vaccine Composition on Basis Thereof
CN112251461B (zh) * 2020-10-22 2022-08-23 植链(上海)生物科技有限公司 一种采用本氏烟作为生物反应器制备的轮状病毒疫苗及其制备方法和应用

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HK1245333A1 (zh) 2018-08-24
CA2974438A1 (en) 2016-07-28
WO2016115630A1 (en) 2016-07-28
US20180016561A1 (en) 2018-01-18
ZA201705555B (en) 2020-01-29
MX2017009568A (es) 2018-03-01
AU2016208999A1 (en) 2017-07-13
CN107208072A (zh) 2017-09-26
JP2018504119A (ja) 2018-02-15
KR20170104595A (ko) 2017-09-15
PH12017501330A1 (en) 2017-12-18
EP3256575A1 (en) 2017-12-20
EP3256575A4 (en) 2018-10-31
BR112017014160A2 (pt) 2018-01-02
IL253040A0 (en) 2017-08-31
ES2826402T3 (es) 2021-05-18
JP6841762B2 (ja) 2021-03-10
SG11201705991XA (en) 2017-08-30
EP3256575B1 (en) 2020-07-29
US20190345454A1 (en) 2019-11-14
US10287555B2 (en) 2019-05-14

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