CN101952446B - 蛋白表达系统 - Google Patents

蛋白表达系统 Download PDF

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Publication number
CN101952446B
CN101952446B CN200980105869.XA CN200980105869A CN101952446B CN 101952446 B CN101952446 B CN 101952446B CN 200980105869 A CN200980105869 A CN 200980105869A CN 101952446 B CN101952446 B CN 101952446B
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rna
sequence
gene
virus
expression
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Expired - Fee Related
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Chinese (zh)
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CN101952446A (zh
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弗兰克·塞恩斯伯里
乔治·P·洛莫诺索夫
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PLANT BIOLOGICAL SCIENIC CO Ltd
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PLANT BIOLOGICAL SCIENIC CO Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN200980105869.XA 2008-01-08 2009-01-08 蛋白表达系统 Expired - Fee Related CN101952446B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0800272.7 2008-01-08
GBGB0800272.7A GB0800272D0 (en) 2008-01-08 2008-01-08 Protein expression systems
PCT/GB2009/000060 WO2009087391A1 (en) 2008-01-08 2009-01-08 Protein expression systems

Publications (2)

Publication Number Publication Date
CN101952446A CN101952446A (zh) 2011-01-19
CN101952446B true CN101952446B (zh) 2014-06-11

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CN200980105869.XA Expired - Fee Related CN101952446B (zh) 2008-01-08 2009-01-08 蛋白表达系统

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US (5) US8674084B2 (enExample)
EP (1) EP2240589B1 (enExample)
JP (1) JP5745857B2 (enExample)
KR (1) KR101602972B1 (enExample)
CN (1) CN101952446B (enExample)
AU (1) AU2009203608B2 (enExample)
BR (1) BRPI0906509A2 (enExample)
CA (1) CA2711895C (enExample)
DK (1) DK2240589T3 (enExample)
ES (1) ES2537198T3 (enExample)
GB (1) GB0800272D0 (enExample)
IL (1) IL206606A (enExample)
MX (1) MX2010007336A (enExample)
NZ (1) NZ586939A (enExample)
PT (1) PT2240589E (enExample)
RU (1) RU2539793C2 (enExample)
WO (1) WO2009087391A1 (enExample)
ZA (1) ZA201005245B (enExample)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
ES2554703T3 (es) 2007-11-27 2015-12-22 Medicago Inc. Partículas recombinantes semejantes al virus de la influenza (VLP) producidas en plantas transgénicas que expresan hemaglutinina
GB0800272D0 (en) * 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
CN105753948A (zh) 2008-07-08 2016-07-13 麦迪卡格公司 可溶性重组流感抗原
EP2445928B1 (en) 2009-06-24 2018-03-28 Medicago Inc. Chimeric influenza virus-like particles comprising hemagglutinin
GB201002434D0 (en) * 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source
CA2815887C (en) * 2010-11-04 2013-12-10 Medicago Inc. Plant expression system
JP6073247B2 (ja) 2011-01-17 2017-02-01 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物で核酸を発現させるベクター
EP2665818B1 (en) 2011-01-17 2017-03-22 Philip Morris Products S.a.s. Protein expression in plants
CN103930435A (zh) 2011-09-30 2014-07-16 麦迪卡格公司 增加植物中病毒样颗粒的产率
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
JP6390846B2 (ja) * 2011-11-11 2018-09-19 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム ニコチアナタバカムにおいて生成される血球凝集素を含むインフルエンザウイルス様粒子（ｖｌｐ）
SG10201810400TA (en) * 2012-09-05 2018-12-28 Medicago Inc Picornavirus-like particle production in plants
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
WO2014153674A1 (en) 2013-03-28 2014-10-02 Medicago Inc. Influenza virus-like particle production in plants
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
DK3092309T3 (da) * 2014-01-10 2021-04-12 Medicago Inc Cpmv-forstærkerelementer
US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements
US11959088B2 (en) 2014-07-11 2024-04-16 Aramis Biotechnologies Inc. Modifying protein production in plants
US10287555B2 (en) 2015-01-23 2019-05-14 Mitsubishi Tanabe Pharma Corporation Rotavirus-like particle production in plants
WO2017000074A1 (en) 2015-07-02 2017-01-05 Medicago Inc. Jasmonic acid pathway activator
BR112019015848A2 (pt) * 2017-02-02 2020-03-31 Duke University Constructo de dna, vetor, célula, planta, e, método de controle da expressão de um polipeptídeo heterólogo em uma célula
GB201721600D0 (en) 2017-12-21 2018-02-07 Plant Bioscience Ltd Metabolic engineering
RU2020132255A (ru) 2018-03-14 2022-04-15 Медикаго Инк. Энхансер экспрессии в растениях
GB201808617D0 (en) 2018-05-25 2018-07-11 Plant Bioscience Ltd Scaffold modification
WO2020033416A1 (en) * 2018-08-06 2020-02-13 Northwestern University Combined transcription and translation platform derived from plant chloroplasts
CA3133166A1 (en) * 2019-03-14 2020-09-17 Mitsubishi Tanabe Pharma Corporation Endogenous plant expression enhancer
GB201908431D0 (en) 2019-06-12 2019-07-24 Plant Bioscience Ltd Biosynthetic genes and polypeptides
WO2020256099A1 (ja) * 2019-06-21 2020-12-24 国立大学法人大阪大学 外来遺伝子を安定的に保持する人工組換えｒｎａウイルスの作製方法
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
CN110468129B (zh) * 2019-08-08 2023-03-24 四川农业大学 一种基于cmv rna2的体外干扰黄瓜花叶病的制剂、其制备方法与应用
CN111777670B (zh) * 2020-06-19 2021-07-30 东北农业大学 一种pH调节自组装抗菌肽及制备方法和应用
CN117479831A (zh) 2021-06-09 2024-01-30 南特知识产权控股有限责任公司 用于在植物中生产感兴趣的蛋白质的方法和系统
CN114181969B (zh) * 2021-12-09 2024-05-28 苏州大学 一种制备包裹外源蛋白的多角体的方法
GB202209501D0 (en) 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
CN121195070A (zh) 2023-04-03 2025-12-23 斯威夫特制药有限责任公司 用于在植物中高效异源基因表达的反式互补载体系统
KR20250082120A (ko) * 2023-11-29 2025-06-09 서강대학교산학협력단 검은눈콩 모자이크 바이러스의 바이러스 유사 입자의 제조방법 및 이의 활용

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007135480A1 (en) * 2006-05-22 2007-11-29 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19701141C1 (de) * 1997-01-16 1998-04-09 Hoechst Ag Genkonstrukte für durch Proteasen aktivierbare Wirksubstanzen
AU2001273738B2 (en) 2000-06-13 2004-09-02 The University Of Queensland Expression modulating sequences
GB0800272D0 (en) 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
DK3092309T3 (da) 2014-01-10 2021-04-12 Medicago Inc Cpmv-forstærkerelementer
US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007135480A1 (en) * 2006-05-22 2007-11-29 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Daiki Matsuda， et al.Expression of the Two Nested Overlapping Reading Frames of Turnip Yellow Mosaic Virus RNA Is Enhanced by a 5" Cap and by 5"and 3"Viral Sequences.《JOURNAL OF VIROLOGY》.2004, *
J Wellink,et al.MUTATIONAL ANALYSIS OF AUG CODONS OF COWPEA MOSAIC VIRUS-M RNA.《Biochimie》.1993, *

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Publication number Publication date
KR101602972B1 (ko) 2016-03-11
US20140250549A1 (en) 2014-09-04
AU2009203608A1 (en) 2009-07-16
JP2011509081A (ja) 2011-03-24
MX2010007336A (es) 2010-08-16
NZ586939A (en) 2012-08-31
PT2240589E (pt) 2015-06-18
US20210155947A1 (en) 2021-05-27
JP5745857B2 (ja) 2015-07-08
IL206606A (en) 2015-11-30
US20100287670A1 (en) 2010-11-11
GB0800272D0 (en) 2008-02-13
RU2539793C2 (ru) 2015-01-27
EP2240589B1 (en) 2015-03-04
WO2009087391A1 (en) 2009-07-16
EP2240589A1 (en) 2010-10-20
ES2537198T3 (es) 2015-06-03
US10947549B2 (en) 2021-03-16
KR20100109950A (ko) 2010-10-11
CA2711895C (en) 2016-08-09
ZA201005245B (en) 2012-01-25
US8674084B2 (en) 2014-03-18
US20200392522A1 (en) 2020-12-17
US10822613B2 (en) 2020-11-03
AU2009203608B2 (en) 2014-03-27
US20180030462A1 (en) 2018-02-01
CN101952446A (zh) 2011-01-19
BRPI0906509A2 (pt) 2015-12-01
RU2010133217A (ru) 2012-02-20
CA2711895A1 (en) 2009-07-16
IL206606A0 (en) 2010-12-30
DK2240589T3 (en) 2015-05-04

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