AU2009203608B2 - Protein expression systems - Google Patents
Protein expression systems Download PDFInfo
- Publication number
- AU2009203608B2 AU2009203608B2 AU2009203608A AU2009203608A AU2009203608B2 AU 2009203608 B2 AU2009203608 B2 AU 2009203608B2 AU 2009203608 A AU2009203608 A AU 2009203608A AU 2009203608 A AU2009203608 A AU 2009203608A AU 2009203608 B2 AU2009203608 B2 AU 2009203608B2
- Authority
- AU
- Australia
- Prior art keywords
- rna
- gene
- sequence
- protein
- initiation site
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 313
- 230000014509 gene expression Effects 0.000 title claims abstract description 243
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 188
- 230000000977 initiatory effect Effects 0.000 claims abstract description 185
- 239000013598 vector Substances 0.000 claims abstract description 113
- 238000000034 method Methods 0.000 claims abstract description 81
- 108700028146 Genetic Enhancer Elements Proteins 0.000 claims abstract description 69
- 241000723655 Cowpea mosaic virus Species 0.000 claims abstract description 36
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 26
- 101150084101 RNA2 gene Proteins 0.000 claims abstract description 23
- 101100353432 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRP2 gene Proteins 0.000 claims abstract description 23
- 241001493065 dsRNA viruses Species 0.000 claims abstract description 23
- 230000014621 translational initiation Effects 0.000 claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims description 149
- 239000002773 nucleotide Substances 0.000 claims description 40
- 125000003729 nucleotide group Chemical group 0.000 claims description 40
- 241000700605 Viruses Species 0.000 claims description 34
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 33
- 230000030279 gene silencing Effects 0.000 claims description 31
- 241000961587 Secoviridae Species 0.000 claims description 29
- 238000013519 translation Methods 0.000 claims description 27
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims description 26
- 108020004414 DNA Proteins 0.000 claims description 25
- 230000035772 mutation Effects 0.000 claims description 25
- 241000723607 Comovirus Species 0.000 claims description 23
- 238000012226 gene silencing method Methods 0.000 claims description 19
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 17
- 108020005345 3' Untranslated Regions Proteins 0.000 claims description 15
- 238000003780 insertion Methods 0.000 claims description 15
- 230000037431 insertion Effects 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 12
- 230000009261 transgenic effect Effects 0.000 claims description 12
- 230000002708 enhancing effect Effects 0.000 claims description 11
- 230000001965 increasing effect Effects 0.000 claims description 11
- 239000003623 enhancer Substances 0.000 claims description 9
- 241000238631 Hexapoda Species 0.000 claims description 8
- 108700026244 Open Reading Frames Proteins 0.000 claims description 6
- 108010058731 nopaline synthase Proteins 0.000 claims description 4
- 238000003306 harvesting Methods 0.000 claims description 3
- 230000000052 comparative effect Effects 0.000 claims 1
- 108091081024 Start codon Proteins 0.000 abstract description 42
- 238000009825 accumulation Methods 0.000 abstract description 9
- 238000012217 deletion Methods 0.000 abstract description 9
- 230000037430 deletion Effects 0.000 abstract description 9
- 230000029812 viral genome replication Effects 0.000 abstract description 5
- 235000018102 proteins Nutrition 0.000 description 146
- 239000005090 green fluorescent protein Substances 0.000 description 56
- 239000013612 plasmid Substances 0.000 description 54
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 50
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 50
- 210000004027 cell Anatomy 0.000 description 46
- 210000001519 tissue Anatomy 0.000 description 38
- 239000013604 expression vector Substances 0.000 description 36
- 150000007523 nucleic acids Chemical class 0.000 description 29
- 238000010367 cloning Methods 0.000 description 28
- 230000000694 effects Effects 0.000 description 27
- 238000004519 manufacturing process Methods 0.000 description 27
- 102000039446 nucleic acids Human genes 0.000 description 27
- 108020004707 nucleic acids Proteins 0.000 description 27
- 230000014616 translation Effects 0.000 description 27
- 241000589158 Agrobacterium Species 0.000 description 26
- 230000009466 transformation Effects 0.000 description 24
- 230000010474 transient expression Effects 0.000 description 24
- 238000001764 infiltration Methods 0.000 description 22
- 230000003321 amplification Effects 0.000 description 21
- 230000000295 complement effect Effects 0.000 description 21
- 238000003199 nucleic acid amplification method Methods 0.000 description 21
- 101710132601 Capsid protein Proteins 0.000 description 19
- 230000010076 replication Effects 0.000 description 18
- 230000008595 infiltration Effects 0.000 description 16
- 230000000692 anti-sense effect Effects 0.000 description 15
- 108090000765 processed proteins & peptides Proteins 0.000 description 13
- 108091034117 Oligonucleotide Proteins 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 12
- 230000010354 integration Effects 0.000 description 12
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 12
- 108010076039 Polyproteins Proteins 0.000 description 11
- 238000010790 dilution Methods 0.000 description 11
- 239000012895 dilution Substances 0.000 description 11
- 238000002703 mutagenesis Methods 0.000 description 11
- 231100000350 mutagenesis Toxicity 0.000 description 11
- 230000009467 reduction Effects 0.000 description 11
- 108700019146 Transgenes Proteins 0.000 description 10
- 108091023045 Untranslated Region Proteins 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 10
- 230000001404 mediated effect Effects 0.000 description 10
- 230000008901 benefit Effects 0.000 description 9
- 239000002245 particle Substances 0.000 description 9
- 230000002829 reductive effect Effects 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 239000000284 extract Substances 0.000 description 8
- 239000012634 fragment Substances 0.000 description 8
- 230000001939 inductive effect Effects 0.000 description 8
- 239000003550 marker Substances 0.000 description 8
- 102000004196 processed proteins & peptides Human genes 0.000 description 8
- 230000003612 virological effect Effects 0.000 description 8
- 241000207746 Nicotiana benthamiana Species 0.000 description 7
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 230000004927 fusion Effects 0.000 description 7
- 230000006872 improvement Effects 0.000 description 7
- 229920001184 polypeptide Polymers 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- 230000001052 transient effect Effects 0.000 description 7
- 108020004705 Codon Proteins 0.000 description 6
- 240000008042 Zea mays Species 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 102200078107 rs387906770 Human genes 0.000 description 6
- 238000002741 site-directed mutagenesis Methods 0.000 description 6
- 241000723596 Bean pod mottle virus Species 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 230000001976 improved effect Effects 0.000 description 5
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 241000701447 unidentified baculovirus Species 0.000 description 5
- 239000013603 viral vector Substances 0.000 description 5
- 108020003589 5' Untranslated Regions Proteins 0.000 description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- 241000701489 Cauliflower mosaic virus Species 0.000 description 4
- 101710094648 Coat protein Proteins 0.000 description 4
- 241000723664 Cowpea severe mosaic virus Species 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 4
- 101710125418 Major capsid protein Proteins 0.000 description 4
- 101710141454 Nucleoprotein Proteins 0.000 description 4
- 101710083689 Probable capsid protein Proteins 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 241000723661 Red clover mottle virus Species 0.000 description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000000635 electron micrograph Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 239000004009 herbicide Substances 0.000 description 4
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 235000009973 maize Nutrition 0.000 description 4
- 230000017074 necrotic cell death Effects 0.000 description 4
- 230000037039 plant physiology Effects 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- 101100437118 Arabidopsis thaliana AUG1 gene Proteins 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 244000020551 Helianthus annuus Species 0.000 description 3
- 235000003222 Helianthus annuus Nutrition 0.000 description 3
- 241000209510 Liliopsida Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 108020005091 Replication Origin Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 240000006394 Sorghum bicolor Species 0.000 description 3
- 241000723658 Squash mosaic virus Species 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 241000710145 Tomato bushy stunt virus Species 0.000 description 3
- 244000098338 Triticum aestivum Species 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000013599 cloning vector Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 239000000287 crude extract Substances 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 230000009368 gene silencing by RNA Effects 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- -1 phosphinotricin Chemical compound 0.000 description 3
- 238000001742 protein purification Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 101150029062 15 gene Proteins 0.000 description 2
- 101150055869 25 gene Proteins 0.000 description 2
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical class O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 2
- 244000144725 Amygdalus communis Species 0.000 description 2
- 235000011437 Amygdalus communis Nutrition 0.000 description 2
- 244000226021 Anacardium occidentale Species 0.000 description 2
- 244000099147 Ananas comosus Species 0.000 description 2
- 235000007119 Ananas comosus Nutrition 0.000 description 2
- 241000723604 Andean potato mottle virus Species 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 241000610275 Bean rugose mosaic virus Species 0.000 description 2
- 108090000565 Capsid Proteins Proteins 0.000 description 2
- 235000009467 Carica papaya Nutrition 0.000 description 2
- 240000006432 Carica papaya Species 0.000 description 2
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 2
- 244000020518 Carthamus tinctorius Species 0.000 description 2
- 239000005496 Chlorsulfuron Substances 0.000 description 2
- 241000207199 Citrus Species 0.000 description 2
- 235000013162 Cocos nucifera Nutrition 0.000 description 2
- 244000060011 Cocos nucifera Species 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 241000723377 Coffea Species 0.000 description 2
- 101100256584 Dictyostelium discoideum selk gene Proteins 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 244000078127 Eleusine coracana Species 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- 239000005562 Glyphosate Substances 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
- 241000208467 Macadamia Species 0.000 description 2
- 235000014826 Mangifera indica Nutrition 0.000 description 2
- 240000007228 Mangifera indica Species 0.000 description 2
- 240000003183 Manihot esculenta Species 0.000 description 2
- 240000004658 Medicago sativa Species 0.000 description 2
- 101000906396 Mus musculus Glutathione S-transferase Mu 5 Proteins 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 240000007817 Olea europaea Species 0.000 description 2
- 235000007199 Panicum miliaceum Nutrition 0.000 description 2
- 244000025272 Persea americana Species 0.000 description 2
- 235000008673 Persea americana Nutrition 0.000 description 2
- 241000723762 Potato virus Y Species 0.000 description 2
- 241000034975 Radish mosaic virus Species 0.000 description 2
- 101150098459 SELENOK gene Proteins 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- 244000082988 Secale cereale Species 0.000 description 2
- 240000005498 Setaria italica Species 0.000 description 2
- 108020004459 Small interfering RNA Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 244000299461 Theobroma cacao Species 0.000 description 2
- 235000009470 Theobroma cacao Nutrition 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 241000219977 Vigna Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000009418 agronomic effect Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 230000010310 bacterial transformation Effects 0.000 description 2
- 244000022203 blackseeded proso millet Species 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 2
- 235000020971 citrus fruits Nutrition 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 2
- 241001233957 eudicotyledons Species 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
- 229940097068 glyphosate Drugs 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 208000002672 hepatitis B Diseases 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 208000006278 hypochromic anemia Diseases 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 239000003999 initiator Substances 0.000 description 2
- 101150066555 lacZ gene Proteins 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 229960000485 methotrexate Drugs 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 210000003705 ribosome Anatomy 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
- 229960000268 spectinomycin Drugs 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- ZAGNMMRDHSEOPE-UHFFFAOYSA-N (2-chlorophenyl) n-methylcarbamate Chemical compound CNC(=O)OC1=CC=CC=C1Cl ZAGNMMRDHSEOPE-UHFFFAOYSA-N 0.000 description 1
- RVLCUCVJZVRNDC-IMJSIDKUSA-N 2-[(2s,5s)-5-methyl-3,6-dioxopiperazin-2-yl]acetic acid Chemical compound C[C@@H]1NC(=O)[C@H](CC(O)=O)NC1=O RVLCUCVJZVRNDC-IMJSIDKUSA-N 0.000 description 1
- 101150098072 20 gene Proteins 0.000 description 1
- 101150110188 30 gene Proteins 0.000 description 1
- 101150096316 5 gene Proteins 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 235000001274 Anacardium occidentale Nutrition 0.000 description 1
- 102400000068 Angiostatin Human genes 0.000 description 1
- 108010079709 Angiostatins Proteins 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 101710151325 B2 protein Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091032955 Bacterial small RNA Proteins 0.000 description 1
- 235000021533 Beta vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 241000702697 Bluetongue virus 10 Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 244000178993 Brassica juncea Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 240000008100 Brassica rapa Species 0.000 description 1
- 241000220243 Brassica sp. Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 102000000584 Calmodulin Human genes 0.000 description 1
- 108010041952 Calmodulin Proteins 0.000 description 1
- 241001515826 Cassava vein mosaic virus Species 0.000 description 1
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 1
- 241001218361 Cheravirus Species 0.000 description 1
- 244000260524 Chrysanthemum balsamita Species 0.000 description 1
- 235000005633 Chrysanthemum balsamita Nutrition 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 102100024458 Cyclin-dependent kinase inhibitor 2A Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 102100038284 Cytospin-B Human genes 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 241000006867 Discosoma Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 235000007349 Eleusine coracana Nutrition 0.000 description 1
- 235000013499 Eleusine coracana subsp coracana Nutrition 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000723648 Fabavirus Species 0.000 description 1
- 241000218218 Ficus <angiosperm> Species 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 240000000047 Gossypium barbadense Species 0.000 description 1
- 235000009429 Gossypium barbadense Nutrition 0.000 description 1
- 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 102100026122 High affinity immunoglobulin gamma Fc receptor I Human genes 0.000 description 1
- 108091006054 His-tagged proteins Proteins 0.000 description 1
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 description 1
- 101000913074 Homo sapiens High affinity immunoglobulin gamma Fc receptor I Proteins 0.000 description 1
- 102000009490 IgG Receptors Human genes 0.000 description 1
- 108010073807 IgG Receptors Proteins 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 102000012745 Immunoglobulin Subunits Human genes 0.000 description 1
- 108010079585 Immunoglobulin Subunits Proteins 0.000 description 1
- 108020005350 Initiator Codon Proteins 0.000 description 1
- 101710125507 Integrase/recombinase Proteins 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 108010092277 Leptin Proteins 0.000 description 1
- 102000016267 Leptin Human genes 0.000 description 1
- 235000004456 Manihot esculenta Nutrition 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241000219828 Medicago truncatula Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 101100365516 Mus musculus Psat1 gene Proteins 0.000 description 1
- 241000234295 Musa Species 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 241000723638 Nepovirus Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 101710144121 Non-structural protein 5 Proteins 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 235000002725 Olea europaea Nutrition 0.000 description 1
- 241000209046 Pennisetum Species 0.000 description 1
- 244000115721 Pennisetum typhoides Species 0.000 description 1
- 235000007195 Pennisetum typhoides Nutrition 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 101800001092 Protein 3B Proteins 0.000 description 1
- 241000508269 Psidium Species 0.000 description 1
- 240000001679 Psidium guajava Species 0.000 description 1
- 235000013929 Psidium pyriferum Nutrition 0.000 description 1
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 1
- 101150023114 RNA1 gene Proteins 0.000 description 1
- 108050002653 Retinoblastoma protein Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 241000702670 Rotavirus Species 0.000 description 1
- 241000209051 Saccharum Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241001596272 Sadwavirus Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 235000008515 Setaria glauca Nutrition 0.000 description 1
- 235000007226 Setaria italica Nutrition 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 235000007230 Sorghum bicolor Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 101900024951 Tomato bushy stunt virus RNA silencing suppressor p19 Proteins 0.000 description 1
- 241000711484 Transmissible gastroenteritis virus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000861887 Turnip ringspot virus Species 0.000 description 1
- 101800003106 VPg Proteins 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 101800001476 Viral genome-linked protein Proteins 0.000 description 1
- 108050006628 Viral movement proteins Proteins 0.000 description 1
- 101800001133 Viral protein genome-linked Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229940126575 aminoglycoside Drugs 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000036436 anti-hiv Effects 0.000 description 1
- 108010038239 aspartyl-alanyl-diketopiperazine Proteins 0.000 description 1
- FZCSTZYAHCUGEM-UHFFFAOYSA-N aspergillomarasmine B Natural products OC(=O)CNC(C(O)=O)CNC(C(O)=O)CC(O)=O FZCSTZYAHCUGEM-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000007622 bioinformatic analysis Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229940027138 cambia Drugs 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 235000020226 cashew nut Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000007771 core particle Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229940039781 leptin Drugs 0.000 description 1
- NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 230000001293 nucleolytic effect Effects 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000002252 panizo Nutrition 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 108010054624 red fluorescent protein Proteins 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 101150087667 spk1 gene Proteins 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 235000020354 squash Nutrition 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 230000007502 viral entry Effects 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000006810 virus induced RNAi Effects 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/08—RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Virology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0800272.7 | 2008-01-08 | ||
| GBGB0800272.7A GB0800272D0 (en) | 2008-01-08 | 2008-01-08 | Protein expression systems |
| PCT/GB2009/000060 WO2009087391A1 (en) | 2008-01-08 | 2009-01-08 | Protein expression systems |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2009203608A1 AU2009203608A1 (en) | 2009-07-16 |
| AU2009203608B2 true AU2009203608B2 (en) | 2014-03-27 |
Family
ID=39111256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2009203608A Ceased AU2009203608B2 (en) | 2008-01-08 | 2009-01-08 | Protein expression systems |
Country Status (18)
| Country | Link |
|---|---|
| US (5) | US8674084B2 (enExample) |
| EP (1) | EP2240589B1 (enExample) |
| JP (1) | JP5745857B2 (enExample) |
| KR (1) | KR101602972B1 (enExample) |
| CN (1) | CN101952446B (enExample) |
| AU (1) | AU2009203608B2 (enExample) |
| BR (1) | BRPI0906509A2 (enExample) |
| CA (1) | CA2711895C (enExample) |
| DK (1) | DK2240589T3 (enExample) |
| ES (1) | ES2537198T3 (enExample) |
| GB (1) | GB0800272D0 (enExample) |
| IL (1) | IL206606A (enExample) |
| MX (1) | MX2010007336A (enExample) |
| NZ (1) | NZ586939A (enExample) |
| PT (1) | PT2240589E (enExample) |
| RU (1) | RU2539793C2 (enExample) |
| WO (1) | WO2009087391A1 (enExample) |
| ZA (1) | ZA201005245B (enExample) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2615372A1 (en) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
| ES2554703T3 (es) | 2007-11-27 | 2015-12-22 | Medicago Inc. | Partículas recombinantes semejantes al virus de la influenza (VLP) producidas en plantas transgénicas que expresan hemaglutinina |
| GB0800272D0 (en) * | 2008-01-08 | 2008-02-13 | Plant Bioscience Ltd | Protein expression systems |
| CN105753948A (zh) | 2008-07-08 | 2016-07-13 | 麦迪卡格公司 | 可溶性重组流感抗原 |
| EP2445928B1 (en) | 2009-06-24 | 2018-03-28 | Medicago Inc. | Chimeric influenza virus-like particles comprising hemagglutinin |
| GB201002434D0 (en) * | 2010-02-12 | 2010-03-31 | Plant Bioscience Ltd | Non-infectious RNA source |
| CA2815887C (en) * | 2010-11-04 | 2013-12-10 | Medicago Inc. | Plant expression system |
| JP6073247B2 (ja) | 2011-01-17 | 2017-02-01 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | 植物で核酸を発現させるベクター |
| EP2665818B1 (en) | 2011-01-17 | 2017-03-22 | Philip Morris Products S.a.s. | Protein expression in plants |
| CN103930435A (zh) | 2011-09-30 | 2014-07-16 | 麦迪卡格公司 | 增加植物中病毒样颗粒的产率 |
| US11390878B2 (en) | 2011-09-30 | 2022-07-19 | Medicago Inc. | Increasing protein yield in plants |
| JP6390846B2 (ja) * | 2011-11-11 | 2018-09-19 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | ニコチアナタバカムにおいて生成される血球凝集素を含むインフルエンザウイルス様粒子（ｖｌｐ） |
| SG10201810400TA (en) * | 2012-09-05 | 2018-12-28 | Medicago Inc | Picornavirus-like particle production in plants |
| KR101449155B1 (ko) * | 2012-12-06 | 2014-10-13 | 주식회사 바이오앱 | 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열 |
| WO2014153674A1 (en) | 2013-03-28 | 2014-10-02 | Medicago Inc. | Influenza virus-like particle production in plants |
| US12467058B2 (en) | 2013-03-28 | 2025-11-11 | Aramis Biotechnologies Inc. | Influenza virus-like particle production in plants |
| DK3092309T3 (da) * | 2014-01-10 | 2021-04-12 | Medicago Inc | Cpmv-forstærkerelementer |
| US10563213B2 (en) | 2014-03-27 | 2020-02-18 | Medicago Inc. | Modified CPMV enhancer elements |
| US11959088B2 (en) | 2014-07-11 | 2024-04-16 | Aramis Biotechnologies Inc. | Modifying protein production in plants |
| US10287555B2 (en) | 2015-01-23 | 2019-05-14 | Mitsubishi Tanabe Pharma Corporation | Rotavirus-like particle production in plants |
| WO2017000074A1 (en) | 2015-07-02 | 2017-01-05 | Medicago Inc. | Jasmonic acid pathway activator |
| BR112019015848A2 (pt) * | 2017-02-02 | 2020-03-31 | Duke University | Constructo de dna, vetor, célula, planta, e, método de controle da expressão de um polipeptídeo heterólogo em uma célula |
| GB201721600D0 (en) | 2017-12-21 | 2018-02-07 | Plant Bioscience Ltd | Metabolic engineering |
| RU2020132255A (ru) | 2018-03-14 | 2022-04-15 | Медикаго Инк. | Энхансер экспрессии в растениях |
| GB201808617D0 (en) | 2018-05-25 | 2018-07-11 | Plant Bioscience Ltd | Scaffold modification |
| WO2020033416A1 (en) * | 2018-08-06 | 2020-02-13 | Northwestern University | Combined transcription and translation platform derived from plant chloroplasts |
| CA3133166A1 (en) * | 2019-03-14 | 2020-09-17 | Mitsubishi Tanabe Pharma Corporation | Endogenous plant expression enhancer |
| GB201908431D0 (en) | 2019-06-12 | 2019-07-24 | Plant Bioscience Ltd | Biosynthetic genes and polypeptides |
| WO2020256099A1 (ja) * | 2019-06-21 | 2020-12-24 | 国立大学法人大阪大学 | 外来遺伝子を安定的に保持する人工組換えｒｎａウイルスの作製方法 |
| GB201909104D0 (en) | 2019-06-25 | 2019-08-07 | Plant Bioscience Ltd | Transferase enzymes |
| CN110468129B (zh) * | 2019-08-08 | 2023-03-24 | 四川农业大学 | 一种基于cmv rna2的体外干扰黄瓜花叶病的制剂、其制备方法与应用 |
| CN111777670B (zh) * | 2020-06-19 | 2021-07-30 | 东北农业大学 | 一种pH调节自组装抗菌肽及制备方法和应用 |
| CN117479831A (zh) | 2021-06-09 | 2024-01-30 | 南特知识产权控股有限责任公司 | 用于在植物中生产感兴趣的蛋白质的方法和系统 |
| CN114181969B (zh) * | 2021-12-09 | 2024-05-28 | 苏州大学 | 一种制备包裹外源蛋白的多角体的方法 |
| GB202209501D0 (en) | 2022-06-29 | 2022-08-10 | Plant Bioscience Ltd | Biosynthetic enzymes |
| CN121195070A (zh) | 2023-04-03 | 2025-12-23 | 斯威夫特制药有限责任公司 | 用于在植物中高效异源基因表达的反式互补载体系统 |
| KR20250082120A (ko) * | 2023-11-29 | 2025-06-09 | 서강대학교산학협력단 | 검은눈콩 모자이크 바이러스의 바이러스 유사 입자의 제조방법 및 이의 활용 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007135480A1 (en) * | 2006-05-22 | 2007-11-29 | Plant Bioscience Limited | Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19701141C1 (de) * | 1997-01-16 | 1998-04-09 | Hoechst Ag | Genkonstrukte für durch Proteasen aktivierbare Wirksubstanzen |
| AU2001273738B2 (en) | 2000-06-13 | 2004-09-02 | The University Of Queensland | Expression modulating sequences |
| GB0800272D0 (en) | 2008-01-08 | 2008-02-13 | Plant Bioscience Ltd | Protein expression systems |
| DK3092309T3 (da) | 2014-01-10 | 2021-04-12 | Medicago Inc | Cpmv-forstærkerelementer |
| US10563213B2 (en) | 2014-03-27 | 2020-02-18 | Medicago Inc. | Modified CPMV enhancer elements |
-
2008
- 2008-01-08 GB GBGB0800272.7A patent/GB0800272D0/en not_active Ceased
-
2009
- 2009-01-08 MX MX2010007336A patent/MX2010007336A/es active IP Right Grant
- 2009-01-08 CN CN200980105869.XA patent/CN101952446B/zh not_active Expired - Fee Related
- 2009-01-08 EP EP09700649.8A patent/EP2240589B1/en active Active
- 2009-01-08 US US12/812,165 patent/US8674084B2/en active Active
- 2009-01-08 RU RU2010133217/10A patent/RU2539793C2/ru not_active Application Discontinuation
- 2009-01-08 DK DK09700649T patent/DK2240589T3/en active
- 2009-01-08 BR BRPI0906509A patent/BRPI0906509A2/pt not_active Application Discontinuation
- 2009-01-08 JP JP2010541103A patent/JP5745857B2/ja not_active Expired - Fee Related
- 2009-01-08 PT PT97006498T patent/PT2240589E/pt unknown
- 2009-01-08 KR KR1020107017609A patent/KR101602972B1/ko not_active Expired - Fee Related
- 2009-01-08 CA CA2711895A patent/CA2711895C/en active Active
- 2009-01-08 NZ NZ586939A patent/NZ586939A/xx not_active IP Right Cessation
- 2009-01-08 WO PCT/GB2009/000060 patent/WO2009087391A1/en not_active Ceased
- 2009-01-08 AU AU2009203608A patent/AU2009203608B2/en not_active Ceased
- 2009-01-08 ES ES09700649.8T patent/ES2537198T3/es active Active
-
2010
- 2010-06-24 IL IL206606A patent/IL206606A/en active IP Right Grant
- 2010-07-22 ZA ZA2010/05245A patent/ZA201005245B/en unknown
-
2014
- 2014-02-04 US US14/172,301 patent/US20140250549A1/en not_active Abandoned
-
2017
- 2017-08-11 US US15/674,881 patent/US10822613B2/en not_active Expired - Fee Related
-
2020
- 2020-08-31 US US17/007,242 patent/US10947549B2/en active Active
- 2020-09-09 US US17/016,191 patent/US20210155947A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007135480A1 (en) * | 2006-05-22 | 2007-11-29 | Plant Bioscience Limited | Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants |
Non-Patent Citations (5)
| Title |
|---|
| CANIZARES, M. C., et al., Plant Biotechnology Journal, 2006, vol. 4, pages 183-19 * |
| HOLNESS, C., et al., Virology, 1989, vol. 172, pages 311-320 * |
| MATSUDA, D., et al., Journal of Virology, 2004, vol. 78, pages 9325-9335 * |
| SAINSBURY, F., et al., Plant Biotechnology Journal, 2008, vol. 6, pages 82-92 * |
| WELLINK, J., et al., Biochimie, 1993, vol. 75, pages 741-747 * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101602972B1 (ko) | 2016-03-11 |
| US20140250549A1 (en) | 2014-09-04 |
| AU2009203608A1 (en) | 2009-07-16 |
| JP2011509081A (ja) | 2011-03-24 |
| MX2010007336A (es) | 2010-08-16 |
| NZ586939A (en) | 2012-08-31 |
| PT2240589E (pt) | 2015-06-18 |
| US20210155947A1 (en) | 2021-05-27 |
| JP5745857B2 (ja) | 2015-07-08 |
| IL206606A (en) | 2015-11-30 |
| US20100287670A1 (en) | 2010-11-11 |
| GB0800272D0 (en) | 2008-02-13 |
| RU2539793C2 (ru) | 2015-01-27 |
| EP2240589B1 (en) | 2015-03-04 |
| WO2009087391A1 (en) | 2009-07-16 |
| EP2240589A1 (en) | 2010-10-20 |
| ES2537198T3 (es) | 2015-06-03 |
| US10947549B2 (en) | 2021-03-16 |
| KR20100109950A (ko) | 2010-10-11 |
| CA2711895C (en) | 2016-08-09 |
| ZA201005245B (en) | 2012-01-25 |
| CN101952446B (zh) | 2014-06-11 |
| US8674084B2 (en) | 2014-03-18 |
| US20200392522A1 (en) | 2020-12-17 |
| US10822613B2 (en) | 2020-11-03 |
| US20180030462A1 (en) | 2018-02-01 |
| CN101952446A (zh) | 2011-01-19 |
| BRPI0906509A2 (pt) | 2015-12-01 |
| RU2010133217A (ru) | 2012-02-20 |
| CA2711895A1 (en) | 2009-07-16 |
| IL206606A0 (en) | 2010-12-30 |
| DK2240589T3 (en) | 2015-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10947549B2 (en) | Protein expression systems | |
| Lindbo | High-efficiency protein expression in plants from agroinfection-compatible Tobacco mosaic virus expression vectors | |
| Gleba et al. | Engineering viral expression vectors for plants: the ‘full virus’ and the ‘deconstructed virus’ strategies | |
| US20090181460A1 (en) | Bipartite System, Method and Composition for the Constitutive and Inducible Expression of High Levels of Foreign Proteins in Plants | |
| US8936937B2 (en) | System for expression of genes in plants from a virus-based expression vector | |
| JP2004531260A (ja) | 植物における対象とする核酸配列の増幅及び発現に用いる方法及びベクター | |
| AU2005206283B2 (en) | Two-component RNA virus-derived plant expression system | |
| CN109880846B (zh) | 一种用于植物基因组编辑载体、及其构建方法和应用 | |
| CN106460005B (zh) | 修饰的cpmv增强子元件 | |
| KR20190051045A (ko) | 식물에서의 표적화된 게놈 최적화 | |
| RU2326167C2 (ru) | Искусственный промотор для экспрессии последовательностей днк в растительных клетках | |
| CN100577807C (zh) | 用于植物中表皮特异性转基因表达的启动子 | |
| CN110423775B (zh) | 一种水稻基因组中稻瘟病抗性位点dna的编辑修饰方法、编辑载体 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |