CA2711895C - Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus - Google Patents

Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus Download PDF

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Publication number
CA2711895C
CA2711895C CA2711895A CA2711895A CA2711895C CA 2711895 C CA2711895 C CA 2711895C CA 2711895 A CA2711895 A CA 2711895A CA 2711895 A CA2711895 A CA 2711895A CA 2711895 C CA2711895 C CA 2711895C
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rna
gene
sequence
initiation site
protein
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French (fr)
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CA2711895A1 (en
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George Peter Lomonossoff
Frank Sainsbury
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Plant Bioscience Ltd
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Plant Bioscience Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08RNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CA2711895A 2008-01-08 2009-01-08 Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus Active CA2711895C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0800272.7 2008-01-08
GBGB0800272.7A GB0800272D0 (en) 2008-01-08 2008-01-08 Protein expression systems
PCT/GB2009/000060 WO2009087391A1 (en) 2008-01-08 2009-01-08 Protein expression systems

Publications (2)

Publication Number Publication Date
CA2711895A1 CA2711895A1 (en) 2009-07-16
CA2711895C true CA2711895C (en) 2016-08-09

Family

ID=39111256

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2711895A Active CA2711895C (en) 2008-01-08 2009-01-08 Methods for protein production employing expression enhancer sequences derived from the rna-z segment of a comoviridae bipartite rna virus

Country Status (18)

Country Link
US (5) US8674084B2 (enExample)
EP (1) EP2240589B1 (enExample)
JP (1) JP5745857B2 (enExample)
KR (1) KR101602972B1 (enExample)
CN (1) CN101952446B (enExample)
AU (1) AU2009203608B2 (enExample)
BR (1) BRPI0906509A2 (enExample)
CA (1) CA2711895C (enExample)
DK (1) DK2240589T3 (enExample)
ES (1) ES2537198T3 (enExample)
GB (1) GB0800272D0 (enExample)
IL (1) IL206606A (enExample)
MX (1) MX2010007336A (enExample)
NZ (1) NZ586939A (enExample)
PT (1) PT2240589E (enExample)
RU (1) RU2539793C2 (enExample)
WO (1) WO2009087391A1 (enExample)
ZA (1) ZA201005245B (enExample)

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CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EP2238253B1 (en) 2007-11-27 2012-09-12 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
GB0800272D0 (en) 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
CA2730171C (en) 2008-07-08 2016-10-11 Medicago Inc. Soluble recombinant influenza antigens
NZ597401A (en) 2009-06-24 2013-09-27 Medicago Inc Chimeric influenza virus-like particles comprising hemagglutinin
GB201002434D0 (en) * 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source
US20130295609A1 (en) * 2010-11-04 2013-11-07 Medicago Inc. Plant expression system
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
EP2665818B1 (en) 2011-01-17 2017-03-22 Philip Morris Products S.a.s. Protein expression in plants
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
CN116218900A (zh) 2011-09-30 2023-06-06 麦迪卡格公司 增加植物中病毒样颗粒的产率
CA2855252C (en) * 2011-11-11 2023-03-07 Philip Morris Products S.A. Influenza virus-like particles (vlps) comprising hemagglutinin produced in nicotiana tabacum
MX364778B (es) * 2012-09-05 2019-05-06 Medicago Inc Produccion de particulas similares a picornavirus en plantas.
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
ES2803508T3 (es) 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
EP3092309B1 (en) * 2014-01-10 2021-02-24 Medicago Inc. Cpmv enhancer elements
ES2912291T3 (es) 2014-03-27 2022-05-25 Medicago Inc Elementos potenciadores de CPMV modificados
US11959088B2 (en) 2014-07-11 2024-04-16 Aramis Biotechnologies Inc. Modifying protein production in plants
EA201791683A1 (ru) 2015-01-23 2017-12-29 Медикаго Инк. Получение ротавирусоподобных частиц в растениях
JP2018524345A (ja) 2015-07-02 2018-08-30 メディカゴ インコーポレイテッド ジャスモン酸経路アクチベーター
CA3052286A1 (en) * 2017-02-02 2018-08-09 Duke University Compositions and methods for controlling gene expression
GB201721600D0 (en) 2017-12-21 2018-02-07 Plant Bioscience Ltd Metabolic engineering
CN112400021A (zh) 2018-03-14 2021-02-23 麦迪卡格公司 植物表达增强子
GB201808617D0 (en) 2018-05-25 2018-07-11 Plant Bioscience Ltd Scaffold modification
WO2020033416A1 (en) * 2018-08-06 2020-02-13 Northwestern University Combined transcription and translation platform derived from plant chloroplasts
EP3938511A4 (en) * 2019-03-14 2023-02-08 Mitsubishi Tanabe Pharma Corporation Endogenous plant expression enhancer
GB201908431D0 (en) 2019-06-12 2019-07-24 Plant Bioscience Ltd Biosynthetic genes and polypeptides
WO2020256099A1 (ja) * 2019-06-21 2020-12-24 国立大学法人大阪大学 外来遺伝子を安定的に保持する人工組換えrnaウイルスの作製方法
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
CN110468129B (zh) * 2019-08-08 2023-03-24 四川农业大学 一种基于cmv rna2的体外干扰黄瓜花叶病的制剂、其制备方法与应用
CN111777670B (zh) * 2020-06-19 2021-07-30 东北农业大学 一种pH调节自组装抗菌肽及制备方法和应用
CN117479831A (zh) 2021-06-09 2024-01-30 南特知识产权控股有限责任公司 用于在植物中生产感兴趣的蛋白质的方法和系统
CN114181969B (zh) * 2021-12-09 2024-05-28 苏州大学 一种制备包裹外源蛋白的多角体的方法
GB202209501D0 (en) 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
KR20250167005A (ko) 2023-04-03 2025-11-28 스위프트파마 비브이 식물에서 고효율 이종성 유전자 발현을 위한 트랜스-상보성 벡터 시스템
KR20250082120A (ko) * 2023-11-29 2025-06-09 서강대학교산학협력단 검은눈콩 모자이크 바이러스의 바이러스 유사 입자의 제조방법 및 이의 활용

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EP3092309B1 (en) 2014-01-10 2021-02-24 Medicago Inc. Cpmv enhancer elements
ES2912291T3 (es) 2014-03-27 2022-05-25 Medicago Inc Elementos potenciadores de CPMV modificados

Also Published As

Publication number Publication date
NZ586939A (en) 2012-08-31
US20210155947A1 (en) 2021-05-27
BRPI0906509A2 (pt) 2015-12-01
US10947549B2 (en) 2021-03-16
WO2009087391A1 (en) 2009-07-16
GB0800272D0 (en) 2008-02-13
PT2240589E (pt) 2015-06-18
US20140250549A1 (en) 2014-09-04
AU2009203608A1 (en) 2009-07-16
RU2539793C2 (ru) 2015-01-27
RU2010133217A (ru) 2012-02-20
JP2011509081A (ja) 2011-03-24
AU2009203608B2 (en) 2014-03-27
ZA201005245B (en) 2012-01-25
DK2240589T3 (en) 2015-05-04
IL206606A0 (en) 2010-12-30
US20180030462A1 (en) 2018-02-01
US10822613B2 (en) 2020-11-03
KR101602972B1 (ko) 2016-03-11
US20100287670A1 (en) 2010-11-11
CN101952446A (zh) 2011-01-19
IL206606A (en) 2015-11-30
US8674084B2 (en) 2014-03-18
EP2240589A1 (en) 2010-10-20
ES2537198T3 (es) 2015-06-03
JP5745857B2 (ja) 2015-07-08
US20200392522A1 (en) 2020-12-17
CN101952446B (zh) 2014-06-11
MX2010007336A (es) 2010-08-16
CA2711895A1 (en) 2009-07-16
EP2240589B1 (en) 2015-03-04
KR20100109950A (ko) 2010-10-11

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