KR101518085B1 - 카피수 변이 측정, 방법 및 시스템 - Google Patents

카피수 변이 측정, 방법 및 시스템 Download PDF

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Publication number
KR101518085B1
KR101518085B1 KR1020107005860A KR20107005860A KR101518085B1 KR 101518085 B1 KR101518085 B1 KR 101518085B1 KR 1020107005860 A KR1020107005860 A KR 1020107005860A KR 20107005860 A KR20107005860 A KR 20107005860A KR 101518085 B1 KR101518085 B1 KR 101518085B1
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polynucleotide sequence
sample
reaction
amplification
target polynucleotide
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KR20100058566A (ko
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라메쉬 라마크리쉬난
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플루이다임 코포레이션
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Eye Examination Apparatus (AREA)
  • Inert Electrodes (AREA)
  • Control Of Vending Devices And Auxiliary Devices For Vending Devices (AREA)
KR1020107005860A 2007-09-07 2008-09-08 카피수 변이 측정, 방법 및 시스템 Expired - Fee Related KR101518085B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96789707P 2007-09-07 2007-09-07
US60/967,897 2007-09-07
PCT/US2008/075636 WO2009033178A1 (en) 2007-09-07 2008-09-08 Copy number variation determination, methods and systems

Publications (2)

Publication Number Publication Date
KR20100058566A KR20100058566A (ko) 2010-06-03
KR101518085B1 true KR101518085B1 (ko) 2015-05-07

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KR1020107005860A Expired - Fee Related KR101518085B1 (ko) 2007-09-07 2008-09-08 카피수 변이 측정, 방법 및 시스템

Country Status (14)

Country Link
US (3) US8148078B2 (https=)
EP (1) EP2198293B1 (https=)
JP (1) JP5707132B2 (https=)
KR (1) KR101518085B1 (https=)
CN (1) CN101821619B (https=)
AT (1) ATE541946T1 (https=)
AU (1) AU2008295992B2 (https=)
BR (1) BRPI0816393A2 (https=)
CA (1) CA2698545C (https=)
EA (1) EA018555B1 (https=)
ES (1) ES2380844T3 (https=)
IL (1) IL204288A (https=)
MX (1) MX2010002556A (https=)
WO (1) WO2009033178A1 (https=)

Families Citing this family (214)

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Publication number Priority date Publication date Assignee Title
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US7214298B2 (en) * 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US7306672B2 (en) 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
IL147302A0 (en) 1999-06-28 2002-08-14 California Inst Of Techn Microfabricated elastomeric valve and pump systems
US7144616B1 (en) 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US8052792B2 (en) * 2001-04-06 2011-11-08 California Institute Of Technology Microfluidic protein crystallography techniques
US7459022B2 (en) 2001-04-06 2008-12-02 California Institute Of Technology Microfluidic protein crystallography
US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
US20050118073A1 (en) * 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US7378280B2 (en) * 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) * 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
CA2480728A1 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
US8220494B2 (en) * 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
JP2006501056A (ja) 2002-09-25 2006-01-12 カリフォルニア インスティテュート オブ テクノロジー ミクロ流体大規模集積
AU2003299541A1 (en) 2002-10-02 2004-05-25 California Institute Of Technology Microfluidic nucleic acid analysis
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
WO2004103563A2 (en) 2003-05-20 2004-12-02 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
US7407799B2 (en) 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
EP2752874A1 (en) 2004-06-07 2014-07-09 Fluidigm Corporation Method and apparatus for imaging a device under temperature control
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1838431A4 (en) * 2004-12-03 2012-08-22 California Inst Of Techn MICROFLUIDIC DEVICES WITH CIRCUITRY OF CHEMICAL REACTIONS
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
EP1882189A2 (en) 2005-04-20 2008-01-30 Fluidigm Corporation Analysis engine and database for manipulating parameters for fluidic systems on a chip
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US8532930B2 (en) 2005-11-26 2013-09-10 Natera, Inc. Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US20070054293A1 (en) * 2005-08-30 2007-03-08 California Institute Of Technology Microfluidic chaotic mixing systems and methods
WO2007033385A2 (en) * 2005-09-13 2007-03-22 Fluidigm Corporation Microfluidic assay devices and methods
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
US7815868B1 (en) * 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US8828661B2 (en) * 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8055034B2 (en) 2006-09-13 2011-11-08 Fluidigm Corporation Methods and systems for image processing of microfluidic devices
WO2008067552A2 (en) * 2006-11-30 2008-06-05 Fluidigm Corporation Method and apparatus for biological sample analysis
EP2125219B1 (en) * 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CA2698545C (en) 2007-09-07 2014-07-08 Fluidigm Corporation Copy number variation determination, methods and systems
US20090088330A1 (en) * 2007-09-28 2009-04-02 Leproust Emily M Methods And Kits For Producing Labeled Target Nucleic Acids For Use In Array Based Hybridization Applications
US9157116B2 (en) 2008-02-08 2015-10-13 Fluidigm Corporation Combinatorial amplification and detection of nucleic acids
CN102056838B (zh) 2008-04-11 2013-07-03 弗卢丁公司 微流体装置和方法
DE102008019132A1 (de) * 2008-04-16 2009-10-22 Olympus Life Science Research Europa Gmbh Verfahren zur quantitativen Bestimmung der Kopienzahl einer vorbestimmten Sequenz in einer Probe
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
SG2014012777A (en) 2008-07-25 2014-06-27 Fluidigm Corp Method and system for manufacturing integratedfluidic chips
CN102171565B (zh) 2008-08-04 2015-04-29 纳特拉公司 等位基因调用和倍性调用的方法
US8617488B2 (en) 2008-08-07 2013-12-31 Fluidigm Corporation Microfluidic mixing and reaction systems for high efficiency screening
WO2010021936A1 (en) * 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
WO2011120006A1 (en) 2010-03-25 2011-09-29 Auantalife, Inc. A Delaware Corporation Detection system for droplet-based assays
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
US8633015B2 (en) * 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US20120252015A1 (en) * 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
CN102348811A (zh) 2009-01-13 2012-02-08 弗卢伊蒂格姆公司 单细胞核酸分析
US8058630B2 (en) 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods
US8450063B2 (en) * 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
EP3415235B1 (en) 2009-03-23 2025-11-12 Bio-Rad Laboratories, Inc. Manipulation of microfluidic droplets
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9415392B2 (en) 2009-03-24 2016-08-16 The University Of Chicago Slip chip device and methods
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US8551787B2 (en) * 2009-07-23 2013-10-08 Fluidigm Corporation Microfluidic devices and methods for binary mixing
EP2473618B1 (en) 2009-09-02 2015-03-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US20120185176A1 (en) 2009-09-30 2012-07-19 Natera, Inc. Methods for Non-Invasive Prenatal Ploidy Calling
SG169918A1 (en) 2009-10-02 2011-04-29 Fluidigm Corp Microfluidic devices with removable cover and methods of fabrication and application
WO2011053790A2 (en) * 2009-10-30 2011-05-05 Fluidigm Corporation Assay of closely linked targets in fetal diagnosis and coincidence detection assay for genetic analysis
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
CN102686246B (zh) 2009-11-30 2016-04-06 富鲁达公司 微流体装置的再生
EP2524056A4 (en) * 2010-01-15 2013-08-14 Univ British Columbia MULTIPLEX REINFORCEMENT FOR DETECTING NUCLEIC ACID VARIATIONS
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP6155419B2 (ja) 2010-03-25 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 検出用の液滴輸送システム
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
EP2572003A4 (en) 2010-05-18 2016-01-13 Natera Inc METHOD FOR NONINVASIVE PRANATAL PLOIDIE ASSIGNMENT
ES2704303T3 (es) 2010-08-24 2019-03-15 Dana Farber Cancer Inst Inc Procedimientos para la predicción de una respuesta contra el cáncer
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US9353406B2 (en) 2010-10-22 2016-05-31 Fluidigm Corporation Universal probe assay methods
CN103429331B (zh) 2010-11-01 2016-09-28 伯乐生命医学产品有限公司 用于形成乳液的系统
WO2012088456A2 (en) 2010-12-22 2012-06-28 Natera, Inc. Methods for non-invasive prenatal paternity testing
AU2011358564B9 (en) 2011-02-09 2017-07-13 Natera, Inc Methods for non-invasive prenatal ploidy calling
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US8399262B2 (en) 2011-03-23 2013-03-19 Darrel A. Mazzari Biosensor
US9168531B2 (en) 2011-03-24 2015-10-27 Fluidigm Corporation Method for thermal cycling of microfluidic samples
US9347059B2 (en) 2011-04-25 2016-05-24 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
SG194722A1 (en) 2011-05-09 2013-12-30 Fluidigm Corp Probe based nucleic acid detection
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
EP4563709A3 (en) 2011-06-17 2025-09-03 Myriad Genetics, Inc. Methods and materials for assessing allelic imbalance
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2737089B1 (en) 2011-07-29 2017-09-06 Bio-rad Laboratories, Inc. Library characterization by digital assay
WO2013033714A1 (en) * 2011-09-01 2013-03-07 Bio-Rad Laboratories, Inc. Digital assays with reduced measurement uncertainty
EP2758544B1 (en) 2011-09-23 2017-10-18 Academisch Medisch Centrum Materials and methods for prognosis of progression of barrett's esophagus
US9121051B2 (en) 2011-10-31 2015-09-01 Arkray, Inc. Method of determining the abundance of a target nucleotide sequence of a gene of interest
JP5570657B2 (ja) 2011-10-31 2014-08-13 アークレイ株式会社 遺伝子存在量の測定方法
EP2788768A1 (en) 2011-12-10 2014-10-15 Abbott Molecular Inc. Materials and methods for diagnosis of malignant melanoma and prognosis of metastasis of malignant melanoma
CN107267598B (zh) 2011-12-21 2022-03-25 美瑞德生物工程公司 用于评估杂合性丢失的方法与材料
US9617601B2 (en) 2011-12-30 2017-04-11 Abbott Molecular Inc. Materials and methods for diagnosis, prognosis and assessment of therapeutic/prophylactic treatment of prostate cancer
EP2798079B1 (en) 2011-12-30 2016-09-07 Abbott Molecular Inc. Materials and methods for diagnosis of bladder cancer and monitoring recurrence thereof
EP2798080B1 (en) 2011-12-30 2019-02-20 Abbott Molecular Inc. Materials and methods for diagnosis, and prognosis of pancreatobiliary cancer
EP3495817B1 (en) * 2012-02-10 2024-10-16 Bio-Rad Laboratories, Inc. Molecular diagnostic screening assay
WO2013130347A1 (en) 2012-02-23 2013-09-06 The Children's Hospital Corporation Methods for predicting anti-cancer response
CN102586456B (zh) * 2012-03-14 2015-07-01 上海翼和应用生物技术有限公司 一种多重竞争性pcr检测拷贝数变异的方法
EP2852682B1 (en) 2012-05-21 2017-10-04 Fluidigm Corporation Single-particle analysis of particle populations
ES2658867T3 (es) 2012-06-07 2018-03-12 Institut Curie Métodos para detectar la inactivación de la ruta de recombinación homóloga (BRCA1/2) en tumores humanos
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
GB2533006B (en) 2012-09-04 2017-06-07 Guardant Health Inc Systems and methods to detect copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
WO2014089368A1 (en) * 2012-12-05 2014-06-12 Bio-Rad Laboratories, Inc Methods for polymerase chain reaction copy number variation assays
WO2014121240A1 (en) 2013-02-01 2014-08-07 Bio-Rad Laboratories, Inc. Multiplexed digital assay with specific and generic reporters
US20140272963A1 (en) 2013-03-14 2014-09-18 Abbott Molecular Inc. Cell preparations and cell supports and their use in theranosis
WO2014160080A1 (en) 2013-03-14 2014-10-02 Children's Medical Center Corporation Cancer diagnosis, treatment selection and treatment
EP2971117B1 (en) * 2013-03-15 2018-08-08 Bio-Rad Laboratories, Inc. Multiplexed digital assay for variant and normal forms of a gene of interest
EP2970367B8 (en) * 2013-03-15 2018-06-13 Bio-rad Laboratories, Inc. Digital assays with a generic reporter
WO2015013681A1 (en) 2013-07-25 2015-01-29 Bio-Rad Laboratories, Inc. Genetic assays
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
EP3080292B1 (en) 2013-12-09 2022-02-02 Institut Curie Methods for detecting inactivation of the homologous recombination pathway (brca1/2) in human tumors
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
ES2660989T3 (es) * 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
EP3957749A1 (en) 2014-04-21 2022-02-23 Natera, Inc. Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3594360B1 (en) 2014-04-24 2021-06-23 Lucira Health, Inc. Colorimetric detection of nucleic acid amplification
US10077469B2 (en) * 2014-05-02 2018-09-18 Bio-Rad Laboratories, Inc. Pre-amplification assay
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US9994912B2 (en) 2014-07-03 2018-06-12 Abbott Molecular Inc. Materials and methods for assessing progression of prostate cancer
EP4234711B1 (en) 2014-08-15 2025-05-28 Myriad Genetics, Inc. Methods and materials for assessing homologous recombination deficiency
US10612080B2 (en) 2014-09-22 2020-04-07 Roche Molecular Systems, Inc. Digital PCR for non-invasive prenatal testing
JP2016145764A (ja) * 2015-02-09 2016-08-12 株式会社東芝 マイクロ分析パッケージ
SG11201707650SA (en) * 2015-03-24 2017-10-30 Agency For Science Tech And Res (A*Star) Normalization methods for measuring gene copy number and expression
CA2989538A1 (en) * 2015-04-20 2016-10-27 Sung Chun Kim Method for analyzing biomolecule by using external biomolecule as standard material, and kit therefor
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
CN104846103A (zh) * 2015-05-26 2015-08-19 南京杰蒙生物技术有限公司 多重数字pcr技术在染色体非整倍体筛查中的应用
WO2017020024A2 (en) * 2015-07-29 2017-02-02 Progenity, Inc. Systems and methods for genetic analysis
JP2018534942A (ja) * 2015-11-26 2018-11-29 ディーエヌエーイー グループ ホールディングス リミテッド 単一分子対照
WO2017106768A1 (en) 2015-12-17 2017-06-22 Guardant Health, Inc. Methods to determine tumor gene copy number by analysis of cell-free dna
JP6949864B2 (ja) 2016-03-14 2021-10-13 ルシラ ヘルス インコーポレイテッド 生物学的アッセイ試料調製および送り出しのための装置および方法
EP3429543B1 (en) 2016-03-14 2024-11-20 Pfizer Inc. Selectively vented biological assay devices and associated methods
AU2017232340B2 (en) 2016-03-14 2022-04-28 Pfizer Inc. Systems and methods for performing biological assays
ES2927062T3 (es) 2016-03-14 2022-11-02 Lucira Health Inc Dispositivos y procedimientos para modificar propiedades ópticas
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
PT3488443T (pt) 2016-07-20 2021-09-24 BioNTech SE Seleção de neoepítopos como alvos específicos da doença para terapia com eficácia melhorada
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CN107312826A (zh) * 2016-12-22 2017-11-03 华东医药（杭州）基因科技有限公司 一种基于扩增矫正的数字pcr检测方法
US10894976B2 (en) 2017-02-21 2021-01-19 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
CN108373971A (zh) * 2017-03-11 2018-08-07 南京科维思生物科技股份有限公司 用于进行实时数字pcr的方法和装置
CN110741094B (zh) * 2017-03-20 2023-04-11 赛雷纳（中国）医疗科技有限公司 一种用于校正扩增子测序中扩增偏差的方法
WO2018186687A1 (ko) * 2017-04-04 2018-10-11 주식회사 젠큐릭스 생물학적 시료의 핵산 품질을 결정하는 방법
US11080848B2 (en) 2017-04-06 2021-08-03 Lucira Health, Inc. Image-based disease diagnostics using a mobile device
US10549275B2 (en) 2017-09-14 2020-02-04 Lucira Health, Inc. Multiplexed biological assay device with electronic readout
CN113777011B (zh) * 2017-09-14 2024-11-19 辉瑞公司 具有电子读出的复用生物测定装置
WO2019069512A1 (ja) * 2017-10-05 2019-04-11 国立研究開発法人産業技術総合研究所 測定必要個数決定装置、測定必要個数決定プログラム及び測定必要個数決定方法、並びに、測定精度推定装置、測定精度推定プログラム及び測定精度推定方法
EP3697927B1 (en) 2017-10-19 2022-12-14 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
US11753681B2 (en) * 2017-11-03 2023-09-12 University Of Washington Digital nucleic acid amplification using encoded particles
JP2021506342A (ja) 2017-12-14 2021-02-22 ティーエーアイ ダイアグノスティックス インコーポレイテッドＴａｉ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． 移植のための移植片適合性の評価
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
CA3090426A1 (en) 2018-04-14 2019-10-17 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
CN110656159B (zh) * 2018-06-28 2024-01-09 深圳华大生命科学研究院 一种拷贝数变异的检测方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP3884066A2 (en) * 2018-11-19 2021-09-29 Biocartis NV Enhanced detection of low-copy-number nucleic acids in an integrated workflow
USD907232S1 (en) 2018-12-21 2021-01-05 Lucira Health, Inc. Medical testing device
US11294162B2 (en) * 2019-02-07 2022-04-05 Nanotronics Imaging, Inc. Fluorescence microscopy inspection systems, apparatus and methods with darkfield channel
EP3980559A1 (en) 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
DE102019220020A1 (de) * 2019-12-18 2021-06-24 Robert Bosch Gmbh Verfahren und Vorrichtung zum Ermitteln einer in einem Fluid enthaltenen Kopienanzahl einer DNA-Sequenz
CN113462760A (zh) * 2020-03-12 2021-10-01 京东方科技集团股份有限公司 生物芯片的检测方法、装置及设备
USD953561S1 (en) 2020-05-05 2022-05-31 Lucira Health, Inc. Diagnostic device with LED display
USD962470S1 (en) 2020-06-03 2022-08-30 Lucira Health, Inc. Assay device with LCD display
CN111718979B (zh) * 2020-07-24 2021-03-23 嘉兴雅康博医学检验所有限公司 基因扩增参考品及其应用
CN117737210A (zh) * 2022-09-09 2024-03-22 深圳瑞吉生物科技有限公司 一种检测mRNA产品中具有poly A尾的mRNA的比例的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070259351A1 (en) 2006-05-03 2007-11-08 James Chinitz Evaluating Genetic Disorders

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4565026A (en) * 1983-09-15 1986-01-21 Bohme August E Remote release deep trolling system
DE3763259D1 (de) 1986-04-29 1990-07-19 Siemens Ag Verfahren und einrichtung zum erfassen des abbindevorganges in einem anorganischen, waessrigen bindemittelsystem.
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5965362A (en) * 1992-03-04 1999-10-12 The Regents Of The University Of California Comparative genomic hybridization (CGH)
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US5863736A (en) 1997-05-23 1999-01-26 Becton, Dickinson And Company Method, apparatus and computer program products for determining quantities of nucleic acid sequences in samples
DE19736691A1 (de) * 1997-08-22 1999-02-25 Michael Prof Dr Med Giesing Verfahren zur Charakterisierung und Identifizierung disseminierter und metastasierter Krebszellen
US6833242B2 (en) 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6958865B1 (en) 1998-11-12 2005-10-25 California Institute Of Technology Microlicensing particles and applications
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
IL147302A0 (en) 1999-06-28 2002-08-14 California Inst Of Techn Microfabricated elastomeric valve and pump systems
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6929030B2 (en) 1999-06-28 2005-08-16 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7144616B1 (en) 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7306672B2 (en) 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US20020151040A1 (en) * 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US20020012926A1 (en) 2000-03-03 2002-01-31 Mycometrix, Inc. Combinatorial array for nucleic acid analysis
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US20050118073A1 (en) 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
WO2002000343A2 (en) 2000-06-27 2002-01-03 Fluidigm Corporation A microfluidic design automation method and system
US6885982B2 (en) 2000-06-27 2005-04-26 Fluidigm Corporation Object oriented microfluidic design method and system
US6301055B1 (en) 2000-08-16 2001-10-09 California Institute Of Technology Solid immersion lens structures and methods for producing solid immersion lens structures
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US7678547B2 (en) 2000-10-03 2010-03-16 California Institute Of Technology Velocity independent analyte characterization
EP1322936A2 (en) 2000-10-03 2003-07-02 California Institute Of Technology Microfluidic devices and methods of use
US7097809B2 (en) 2000-10-03 2006-08-29 California Institute Of Technology Combinatorial synthesis system
AU1189702A (en) 2000-10-13 2002-04-22 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
US7232109B2 (en) 2000-11-06 2007-06-19 California Institute Of Technology Electrostatic valves for microfluidic devices
WO2002060582A2 (en) 2000-11-16 2002-08-08 Fluidigm Corporation Microfluidic devices for introducing and dispensing fluids from microfluidic systems
US7378280B2 (en) 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US20050196785A1 (en) 2001-03-05 2005-09-08 California Institute Of Technology Combinational array for nucleic acid analysis
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
ATE500051T1 (de) 2001-04-06 2011-03-15 Fluidigm Corp Polymeroberflächenmodifikation
US6802342B2 (en) 2001-04-06 2004-10-12 Fluidigm Corporation Microfabricated fluidic circuit elements and applications
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US7075162B2 (en) 2001-08-30 2006-07-11 Fluidigm Corporation Electrostatic/electrostrictive actuation of elastomer structures using compliant electrodes
JP2005505441A (ja) 2001-10-08 2005-02-24 カリフォルニア インスティテュート オブ テクノロジー 微小加工レンズ、その製造の方法および応用
WO2003031066A1 (en) 2001-10-11 2003-04-17 California Institute Of Technology Devices utilizing self-assembled gel and method of manufacture
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
CA2480728A1 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
US7059348B2 (en) 2002-05-13 2006-06-13 Fluidigm Corporation Drug delivery system
US20060086309A1 (en) 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
JP2006501056A (ja) 2002-09-25 2006-01-12 カリフォルニア インスティテュート オブ テクノロジー ミクロ流体大規模集積
AU2003299541A1 (en) 2002-10-02 2004-05-25 California Institute Of Technology Microfluidic nucleic acid analysis
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
AU2004228678A1 (en) 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
WO2004094020A2 (en) 2003-04-17 2004-11-04 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
WO2004103563A2 (en) 2003-05-20 2004-12-02 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
SG145697A1 (en) 2003-07-28 2008-09-29 Fluidigm Corp Image processing method and system for microfluidic devices
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7042649B2 (en) 2003-08-11 2006-05-09 California Institute Of Technology Microfabricated rubber microscope using soft solid immersion lenses
US20060172408A1 (en) 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
US7407799B2 (en) 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
EP2752874A1 (en) 2004-06-07 2014-07-09 Fluidigm Corporation Method and apparatus for imaging a device under temperature control
EP1838431A4 (en) 2004-12-03 2012-08-22 California Inst Of Techn MICROFLUIDIC DEVICES WITH CIRCUITRY OF CHEMICAL REACTIONS
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
EP1882189A2 (en) 2005-04-20 2008-01-30 Fluidigm Corporation Analysis engine and database for manipulating parameters for fluidic systems on a chip
US20070054293A1 (en) 2005-08-30 2007-03-08 California Institute Of Technology Microfluidic chaotic mixing systems and methods
US8206975B2 (en) 2005-10-28 2012-06-26 California Institute Of Technology Method and device for regulating fluid flow in microfluidic devices
US20070248971A1 (en) 2006-01-26 2007-10-25 California Institute Of Technology Programming microfluidic devices with molecular information
EP1987358A4 (en) 2006-01-26 2013-12-04 California Inst Of Techn MECHANICALLY INTRODUCED TRAPPING OF MOLECULAR INTERACTIONS
TR201910868T4 (tr) * 2006-02-02 2019-08-21 Univ Leland Stanford Junior Dijital analizle invazif olmayan fetal genetik tarama.
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US20070238106A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Systems and methods of determining alleles and/or copy numbers
US8828661B2 (en) 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
US8055034B2 (en) 2006-09-13 2011-11-08 Fluidigm Corporation Methods and systems for image processing of microfluidic devices
WO2008067552A2 (en) 2006-11-30 2008-06-05 Fluidigm Corporation Method and apparatus for biological sample analysis
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008141183A2 (en) 2007-05-09 2008-11-20 Fluidigm Corporation Method and system for crystallization and x-ray diffraction screening
CA2698545C (en) 2007-09-07 2014-07-08 Fluidigm Corporation Copy number variation determination, methods and systems
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
HUE031848T2 (en) 2008-09-20 2017-08-28 Univ Leland Stanford Junior Non-invasive diagnosis of fetal aneuploidy by sequencing
US8058630B2 (en) 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods
AU2010206843B2 (en) 2009-01-20 2015-01-29 The Board Of Trustees Of The Leland Stanford Junior University Single cell gene expression for diagnosis, prognosis and identification of drug targets

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070259351A1 (en) 2006-05-03 2007-11-08 James Chinitz Evaluating Genetic Disorders

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Vogelstein and Kinzler, 1999, Proc Natl Acad Sci USA 96:9236-41

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