KR101518085B1 - 카피수 변이 측정, 방법 및 시스템 - Google Patents

카피수 변이 측정, 방법 및 시스템 Download PDF

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KR101518085B1
KR101518085B1 KR1020107005860A KR20107005860A KR101518085B1 KR 101518085 B1 KR101518085 B1 KR 101518085B1 KR 1020107005860 A KR1020107005860 A KR 1020107005860A KR 20107005860 A KR20107005860 A KR 20107005860A KR 101518085 B1 KR101518085 B1 KR 101518085B1
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Prior art keywords
polynucleotide sequence
sample
reaction
amplification
target polynucleotide
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KR20100058566A (ko
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라메쉬 라마크리쉬난
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플루이다임 코포레이션
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Inert Electrodes (AREA)
  • Control Of Vending Devices And Auxiliary Devices For Vending Devices (AREA)
  • Eye Examination Apparatus (AREA)
KR1020107005860A 2007-09-07 2008-09-08 카피수 변이 측정, 방법 및 시스템 Expired - Fee Related KR101518085B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96789707P 2007-09-07 2007-09-07
US60/967,897 2007-09-07
PCT/US2008/075636 WO2009033178A1 (en) 2007-09-07 2008-09-08 Copy number variation determination, methods and systems

Publications (2)

Publication Number Publication Date
KR20100058566A KR20100058566A (ko) 2010-06-03
KR101518085B1 true KR101518085B1 (ko) 2015-05-07

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KR1020107005860A Expired - Fee Related KR101518085B1 (ko) 2007-09-07 2008-09-08 카피수 변이 측정, 방법 및 시스템

Country Status (14)

Country Link
US (3) US8148078B2 (https=)
EP (1) EP2198293B1 (https=)
JP (1) JP5707132B2 (https=)
KR (1) KR101518085B1 (https=)
CN (1) CN101821619B (https=)
AT (1) ATE541946T1 (https=)
AU (1) AU2008295992B2 (https=)
BR (1) BRPI0816393A2 (https=)
CA (1) CA2698545C (https=)
EA (1) EA018555B1 (https=)
ES (1) ES2380844T3 (https=)
IL (1) IL204288A (https=)
MX (1) MX2010002556A (https=)
WO (1) WO2009033178A1 (https=)

Families Citing this family (214)

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Publication number Priority date Publication date Assignee Title
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US7214298B2 (en) * 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US7459022B2 (en) 2001-04-06 2008-12-02 California Institute Of Technology Microfluidic protein crystallography
US7144616B1 (en) * 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
US8052792B2 (en) * 2001-04-06 2011-11-08 California Institute Of Technology Microfluidic protein crystallography techniques
US7306672B2 (en) * 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
US7601270B1 (en) 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US20050118073A1 (en) * 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
EP1343973B2 (en) 2000-11-16 2020-09-16 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7691333B2 (en) * 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US8220494B2 (en) * 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
JP2006501056A (ja) 2002-09-25 2006-01-12 カリフォルニア インスティテュート オブ テクノロジー ミクロ流体大規模集積
WO2004040001A2 (en) 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
US7604965B2 (en) * 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US8828663B2 (en) * 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
AU2004240944A1 (en) 2003-05-20 2004-12-02 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
US7407799B2 (en) 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
CN103884698B (zh) 2004-06-07 2017-04-12 先锋生物科技股份有限公司 用于微流体器件的光学透镜系统和方法
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
EP1838431A4 (en) * 2004-12-03 2012-08-22 California Inst Of Techn MICROFLUIDIC DEVICES WITH CIRCUITRY OF CHEMICAL REACTIONS
US7883669B2 (en) 2005-04-20 2011-02-08 Fluidigm Corporation Analysis engine and database for manipulating parameters for fluidic systems on a chip
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US8532930B2 (en) 2005-11-26 2013-09-10 Natera, Inc. Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US20070054293A1 (en) * 2005-08-30 2007-03-08 California Institute Of Technology Microfluidic chaotic mixing systems and methods
EP1938101A2 (en) * 2005-09-13 2008-07-02 Fluidigm Corporation Microfluidic assay devices and methods
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7815868B1 (en) * 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US8828661B2 (en) * 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
EP2530167A1 (en) 2006-05-11 2012-12-05 Raindance Technologies, Inc. Microfluidic Devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8055034B2 (en) 2006-09-13 2011-11-08 Fluidigm Corporation Methods and systems for image processing of microfluidic devices
US8473216B2 (en) * 2006-11-30 2013-06-25 Fluidigm Corporation Method and program for performing baseline correction of amplification curves in a PCR experiment
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2009033178A1 (en) 2007-09-07 2009-03-12 Fluidigm Corporation Copy number variation determination, methods and systems
US20090088330A1 (en) * 2007-09-28 2009-04-02 Leproust Emily M Methods And Kits For Producing Labeled Target Nucleic Acids For Use In Array Based Hybridization Applications
WO2009100449A1 (en) 2008-02-08 2009-08-13 Fluidigm Corporation Dynamic array assay methods
WO2009126826A1 (en) 2008-04-11 2009-10-15 Fluidigm Corporation Multilevel microfluidic systems and methods
DE102008019132A1 (de) * 2008-04-16 2009-10-22 Olympus Life Science Research Europa Gmbh Verfahren zur quantitativen Bestimmung der Kopienzahl einer vorbestimmten Sequenz in einer Probe
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
CN104043490A (zh) 2008-07-25 2014-09-17 弗卢丁公司 用于制造集成流体芯片的方法和系统
EP2321642B1 (en) 2008-08-04 2017-01-11 Natera, Inc. Methods for allele calling and ploidy calling
WO2010017210A1 (en) 2008-08-07 2010-02-11 Fluidigm Corporation Microfluidic mixing and reaction systems for high efficiency screening
US20100069250A1 (en) * 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US20130059754A1 (en) * 2011-09-01 2013-03-07 Bio-Rad Laboratories, Inc. Digital assays with reduced measurement uncertainty
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
EP4512526A3 (en) 2008-09-23 2025-06-04 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8633015B2 (en) * 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US20120252015A1 (en) * 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
SG183029A1 (en) 2009-01-13 2012-08-30 Fluidigm Corp Single-cell nucleic acid analysis
US8058630B2 (en) 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods
US8450063B2 (en) * 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
JP5766178B2 (ja) 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ ＳｌｉｐＣｈｉｐ装置および方法
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US8551787B2 (en) * 2009-07-23 2013-10-08 Fluidigm Corporation Microfluidic devices and methods for binary mixing
CA2767056C (en) * 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
WO2011041485A1 (en) 2009-09-30 2011-04-07 Gene Security Network, Inc. Methods for non-invasive prenatal ploidy calling
SG169918A1 (en) 2009-10-02 2011-04-29 Fluidigm Corp Microfluidic devices with removable cover and methods of fabrication and application
WO2011053790A2 (en) * 2009-10-30 2011-05-05 Fluidigm Corporation Assay of closely linked targets in fetal diagnosis and coincidence detection assay for genetic analysis
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
WO2011066588A1 (en) 2009-11-30 2011-06-03 Fluidigm Corporation Microfluidic device regeneration
US20130022973A1 (en) * 2010-01-15 2013-01-24 Hansen Carl L G Multiplex Amplification for the Detection of Nucleic Acid Variations
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
JP2013524169A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴によるアッセイ用の検出システム
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
WO2011146632A1 (en) 2010-05-18 2011-11-24 Gene Security Network Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
WO2012027224A1 (en) 2010-08-24 2012-03-01 Dana-Farber Cancer Institute, Inc. Methods for predicting anti-cancer response
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012054933A2 (en) 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
EP3574990B1 (en) 2010-11-01 2022-04-06 Bio-Rad Laboratories, Inc. System for forming emulsions
CN103608466B (zh) 2010-12-22 2020-09-18 纳特拉公司 非侵入性产前亲子鉴定方法
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
EP3412778A1 (en) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
CN103534360A (zh) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 借助对信号的组合使用进行的多重数字分析
US8399262B2 (en) 2011-03-23 2013-03-19 Darrel A. Mazzari Biosensor
US9168531B2 (en) 2011-03-24 2015-10-27 Fluidigm Corporation Method for thermal cycling of microfluidic samples
EP3395957B1 (en) 2011-04-25 2020-08-12 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US8809238B2 (en) 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
EP3216872B1 (en) 2011-06-02 2020-04-01 Bio-Rad Laboratories, Inc. Enzyme quantification
JP6117194B2 (ja) 2011-06-17 2017-04-19 ミリアド・ジェネティックス・インコーポレイテッド アレル不均衡を評価するための方法および材料
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US20130078632A1 (en) 2011-09-23 2013-03-28 Academisch Medisch Centrum Materials and methods for prognosis of progression of barrett's esophagus
CN104024433B (zh) 2011-10-31 2016-11-16 爱科来株式会社 基因丰度的测定方法
US9121051B2 (en) 2011-10-31 2015-09-01 Arkray, Inc. Method of determining the abundance of a target nucleotide sequence of a gene of interest
EP2788768A1 (en) 2011-12-10 2014-10-15 Abbott Molecular Inc. Materials and methods for diagnosis of malignant melanoma and prognosis of metastasis of malignant melanoma
BR112014015152A2 (pt) 2011-12-21 2017-07-04 Myriad Genetics Inc métodos e materiais para a avaliação da perda de heterozigosidade
WO2013101613A1 (en) 2011-12-30 2013-07-04 Abbott Molecular Inc. Materials and methods for diagnosis, prognosis, monitoring of recurrence, and assessment of therapeutic/prophylactic treatment of pancreatobiliary cancer
ES2601460T3 (es) 2011-12-30 2017-02-15 Abbott Molecular Inc. Materiales y métodos para el diagnóstico del cáncer de vejiga y el control de la recurrencia del mismo
EP2798082B1 (en) 2011-12-30 2017-04-12 Abbott Molecular Inc. Materials and methods for diagnosis, prognosis and assessment of therapeutic/prophylactic treatment of prostate cancer
WO2013120089A1 (en) * 2012-02-10 2013-08-15 Raindance Technologies, Inc. Molecular diagnostic screening assay
CA2864481C (en) 2012-02-23 2020-07-14 Dana-Farber Cancer Institute, Inc. Methods for predicting anti-cancer response
CN102586456B (zh) * 2012-03-14 2015-07-01 上海翼和应用生物技术有限公司 一种多重竞争性pcr检测拷贝数变异的方法
WO2013177206A2 (en) 2012-05-21 2013-11-28 Fluidigm Corporation Single-particle analysis of particle populations
WO2013182645A1 (en) 2012-06-07 2013-12-12 Institut Curie Methods for detecting inactivation of the homologous recombination pathway (brca1/2) in human tumors
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
WO2014089368A1 (en) * 2012-12-05 2014-06-12 Bio-Rad Laboratories, Inc Methods for polymerase chain reaction copy number variation assays
EP2951320B1 (en) 2013-02-01 2018-12-26 Bio-Rad Laboratories, Inc. Multiplexed digital assay with specific and generic reporters
US20140272969A1 (en) 2013-03-14 2014-09-18 Abbott Molecular Inc. Cell preparations and cell supports and their use in theranosis
US10308986B2 (en) 2013-03-14 2019-06-04 Children's Medical Center Corporation Cancer diagnosis, treatment selection and treatment
EP2971117B1 (en) * 2013-03-15 2018-08-08 Bio-Rad Laboratories, Inc. Multiplexed digital assay for variant and normal forms of a gene of interest
EP3372612A1 (en) * 2013-03-15 2018-09-12 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
CN105555972B (zh) 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 遗传测定
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
WO2015086473A1 (en) 2013-12-09 2015-06-18 Institut Curie Methods for detecting inactivation of the homologous recombination pathway (brca1/2) in human tumors
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
CA2945962C (en) 2014-04-21 2023-08-29 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3594360B1 (en) 2014-04-24 2021-06-23 Lucira Health, Inc. Colorimetric detection of nucleic acid amplification
CN105392903B (zh) * 2014-05-02 2021-05-04 生物辐射实验室股份有限公司 预扩增试验
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US9994912B2 (en) 2014-07-03 2018-06-12 Abbott Molecular Inc. Materials and methods for assessing progression of prostate cancer
CA2958801A1 (en) 2014-08-15 2016-02-18 Myriad Genetics, Inc. Methods and materials for assessing homologous recombination deficiency
US10612080B2 (en) * 2014-09-22 2020-04-07 Roche Molecular Systems, Inc. Digital PCR for non-invasive prenatal testing
JP2016145764A (ja) * 2015-02-09 2016-08-12 株式会社東芝 マイクロ分析パッケージ
WO2016153434A1 (en) * 2015-03-24 2016-09-29 Agency For Science, Technology And Research (A*Star) Normalization methods for measuring gene copy number and expression
EP3287530A4 (en) * 2015-04-20 2018-11-21 KIM, Sung-Chun Method for analyzing biomolecule by using external biomolecule as standard material, and kit therefor
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
CN104846103A (zh) * 2015-05-26 2015-08-19 南京杰蒙生物技术有限公司 多重数字pcr技术在染色体非整倍体筛查中的应用
CA2993619A1 (en) * 2015-07-29 2017-02-02 Progenity, Inc. Systems and methods for genetic analysis
CN108699597A (zh) * 2015-11-26 2018-10-23 DNAe集团控股有限公司 单分子对照
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
EP3429752A4 (en) 2016-03-14 2019-10-30 Lucira Health, Inc. SYSTEMS AND METHOD FOR IMPLEMENTING BIOLOGICAL TESTS
EP4477316A3 (en) 2016-03-14 2025-02-19 Pfizer Inc. Selectively vented biological assay devices and associated methods
DK3430378T3 (da) 2016-03-14 2022-10-24 Lucira Health Inc Indretninger og fremgangsmåder til modifikation af optiske egenskaber
CA3214851A1 (en) 2016-03-14 2017-09-21 Lucira Health, Inc. Devices and methods for biological assay sample preparation and delivery
RU2760913C2 (ru) 2016-04-15 2021-12-01 Натера, Инк. Способы выявления рака легкого
SG11201900132QA (en) 2016-07-20 2019-02-27 Biontech Rna Pharmaceuticals Gmbh Selecting neoepitopes as disease-specific targets for therapy with enhanced efficacy
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CN107312826A (zh) * 2016-12-22 2017-11-03 华东医药（杭州）基因科技有限公司 一种基于扩增矫正的数字pcr检测方法
WO2018156418A1 (en) 2017-02-21 2018-08-30 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
CN108373971A (zh) * 2017-03-11 2018-08-07 南京科维思生物科技股份有限公司 用于进行实时数字pcr的方法和装置
WO2018170660A1 (en) * 2017-03-20 2018-09-27 Celula China Med-Technology Co., Ltd. Method of correcting amplification bias in amplicon sequencing
WO2018186687A1 (ko) * 2017-04-04 2018-10-11 주식회사 젠큐릭스 생물학적 시료의 핵산 품질을 결정하는 방법
US11080848B2 (en) 2017-04-06 2021-08-03 Lucira Health, Inc. Image-based disease diagnostics using a mobile device
US10549275B2 (en) 2017-09-14 2020-02-04 Lucira Health, Inc. Multiplexed biological assay device with electronic readout
CN113777011B (zh) 2017-09-14 2024-11-19 辉瑞公司 具有电子读出的复用生物测定装置
JP6971496B2 (ja) * 2017-10-05 2021-11-24 国立研究開発法人産業技術総合研究所 測定必要個数決定のための情報処理装置、方法及びプログラム
US11072820B2 (en) 2017-10-19 2021-07-27 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
US11753681B2 (en) * 2017-11-03 2023-09-12 University Of Washington Digital nucleic acid amplification using encoded particles
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
CN110656159B (zh) * 2018-06-28 2024-01-09 深圳华大生命科学研究院 一种拷贝数变异的检测方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
JP2022513079A (ja) * 2018-11-19 2022-02-07 バイオカルティス エン フェー 統合ワークフローでの低コピー数の核酸の強化された検出
USD907232S1 (en) 2018-12-21 2021-01-05 Lucira Health, Inc. Medical testing device
US11294162B2 (en) * 2019-02-07 2022-04-05 Nanotronics Imaging, Inc. Fluorescence microscopy inspection systems, apparatus and methods with darkfield channel
US12305235B2 (en) 2019-06-06 2025-05-20 Natera, Inc. Methods for detecting immune cell DNA and monitoring immune system
DE102019220020A1 (de) * 2019-12-18 2021-06-24 Robert Bosch Gmbh Verfahren und Vorrichtung zum Ermitteln einer in einem Fluid enthaltenen Kopienanzahl einer DNA-Sequenz
CN113462760A (zh) * 2020-03-12 2021-10-01 京东方科技集团股份有限公司 生物芯片的检测方法、装置及设备
USD953561S1 (en) 2020-05-05 2022-05-31 Lucira Health, Inc. Diagnostic device with LED display
USD962470S1 (en) 2020-06-03 2022-08-30 Lucira Health, Inc. Assay device with LCD display
CN111718979B (zh) * 2020-07-24 2021-03-23 嘉兴雅康博医学检验所有限公司 基因扩增参考品及其应用
CN117737210A (zh) * 2022-09-09 2024-03-22 深圳瑞吉生物科技有限公司 一种检测mRNA产品中具有poly A尾的mRNA的比例的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070259351A1 (en) 2006-05-03 2007-11-08 James Chinitz Evaluating Genetic Disorders

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4565026A (en) * 1983-09-15 1986-01-21 Bohme August E Remote release deep trolling system
DE3763259D1 (de) * 1986-04-29 1990-07-19 Siemens Ag Verfahren und einrichtung zum erfassen des abbindevorganges in einem anorganischen, waessrigen bindemittelsystem.
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5976790A (en) * 1992-03-04 1999-11-02 The Regents Of The University Of California Comparative Genomic Hybridization (CGH)
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6221654B1 (en) * 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US5863736A (en) 1997-05-23 1999-01-26 Becton, Dickinson And Company Method, apparatus and computer program products for determining quantities of nucleic acid sequences in samples
DE19736691A1 (de) * 1997-08-22 1999-02-25 Michael Prof Dr Med Giesing Verfahren zur Charakterisierung und Identifizierung disseminierter und metastasierter Krebszellen
US7214298B2 (en) * 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6540895B1 (en) * 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6833242B2 (en) * 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6958865B1 (en) * 1998-11-12 2005-10-25 California Institute Of Technology Microlicensing particles and applications
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7144616B1 (en) * 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7601270B1 (en) 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7306672B2 (en) * 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
US6929030B2 (en) * 1999-06-28 2005-08-16 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6440706B1 (en) * 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US20020151040A1 (en) * 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
WO2001067369A2 (en) * 2000-03-03 2001-09-13 California Institute Of Technology Combinatorial array for nucleic acid analysis
US20050118073A1 (en) * 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US7867763B2 (en) * 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US7351376B1 (en) * 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7062418B2 (en) * 2000-06-27 2006-06-13 Fluidigm Corporation Computer aided design method and system for developing a microfluidic system
US6885982B2 (en) * 2000-06-27 2005-04-26 Fluidigm Corporation Object oriented microfluidic design method and system
US6301055B1 (en) * 2000-08-16 2001-10-09 California Institute Of Technology Solid immersion lens structures and methods for producing solid immersion lens structures
WO2002023163A1 (en) * 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
AU2002211389A1 (en) * 2000-10-03 2002-04-15 California Institute Of Technology Microfluidic devices and methods of use
US7097809B2 (en) * 2000-10-03 2006-08-29 California Institute Of Technology Combinatorial synthesis system
US7678547B2 (en) * 2000-10-03 2010-03-16 California Institute Of Technology Velocity independent analyte characterization
EP1336097A4 (en) * 2000-10-13 2006-02-01 Fluidigm Corp SAMPLE INJECTION SYSTEM BASED ON A MICROFLUIDIC EQUIPMENT FOR ANALYTICAL EQUIPMENT
US7232109B2 (en) * 2000-11-06 2007-06-19 California Institute Of Technology Electrostatic valves for microfluidic devices
EP1343973B2 (en) * 2000-11-16 2020-09-16 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
AU2002248149A1 (en) * 2000-11-16 2002-08-12 Fluidigm Corporation Microfluidic devices for introducing and dispensing fluids from microfluidic systems
US20050196785A1 (en) * 2001-03-05 2005-09-08 California Institute Of Technology Combinational array for nucleic acid analysis
US6752922B2 (en) * 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US6802342B2 (en) * 2001-04-06 2004-10-12 Fluidigm Corporation Microfabricated fluidic circuit elements and applications
WO2002081183A1 (en) * 2001-04-06 2002-10-17 Fluidigm Corporation Polymer surface modification
EP1384022A4 (en) * 2001-04-06 2004-08-04 California Inst Of Techn NUCLEIC ACID AMPLIFICATION USING MICROFLUID DEVICES
US7075162B2 (en) * 2001-08-30 2006-07-11 Fluidigm Corporation Electrostatic/electrostrictive actuation of elastomer structures using compliant electrodes
WO2003031163A2 (en) * 2001-10-08 2003-04-17 California Institute Of Technology Microfabricated lenses, methods of manufacture thereof, and applications therefor
US7192629B2 (en) * 2001-10-11 2007-03-20 California Institute Of Technology Devices utilizing self-assembled gel and method of manufacture
WO2003048295A1 (en) * 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) * 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
AU2003224817B2 (en) * 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US7059348B2 (en) * 2002-05-13 2006-06-13 Fluidigm Corporation Drug delivery system
US20060086309A1 (en) * 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
JP2006501056A (ja) * 2002-09-25 2006-01-12 カリフォルニア インスティテュート オブ テクノロジー ミクロ流体大規模集積
US8220494B2 (en) * 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
WO2004040001A2 (en) * 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
US7604965B2 (en) * 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US8828663B2 (en) * 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
CA2521171C (en) * 2003-04-03 2013-05-28 Fluidigm Corp. Microfluidic devices and methods of using same
US7476363B2 (en) * 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US20050145496A1 (en) * 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
WO2004094020A2 (en) * 2003-04-17 2004-11-04 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
AU2004240944A1 (en) * 2003-05-20 2004-12-02 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
AU2004261655A1 (en) * 2003-07-28 2005-02-10 Fluidigm Corporation Image processing method and system for microfluidic devices
US7413712B2 (en) * 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7042649B2 (en) * 2003-08-11 2006-05-09 California Institute Of Technology Microfabricated rubber microscope using soft solid immersion lenses
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
US7407799B2 (en) * 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
WO2005080605A2 (en) * 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
CN103884698B (zh) * 2004-06-07 2017-04-12 先锋生物科技股份有限公司 用于微流体器件的光学透镜系统和方法
WO2006060748A2 (en) * 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
EP1838431A4 (en) * 2004-12-03 2012-08-22 California Inst Of Techn MICROFLUIDIC DEVICES WITH CIRCUITRY OF CHEMICAL REACTIONS
US7883669B2 (en) * 2005-04-20 2011-02-08 Fluidigm Corporation Analysis engine and database for manipulating parameters for fluidic systems on a chip
US20070054293A1 (en) * 2005-08-30 2007-03-08 California Institute Of Technology Microfluidic chaotic mixing systems and methods
US8206975B2 (en) * 2005-10-28 2012-06-26 California Institute Of Technology Method and device for regulating fluid flow in microfluidic devices
WO2007089587A2 (en) * 2006-01-26 2007-08-09 California Institute Of Technology Mechanically induced trapping of molecular interactions
US20070248971A1 (en) * 2006-01-26 2007-10-25 California Institute Of Technology Programming microfluidic devices with molecular information
EP3591068A1 (en) * 2006-02-02 2020-01-08 The Board of Trustees of the Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
US7815868B1 (en) * 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US20070238106A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Systems and methods of determining alleles and/or copy numbers
US8828661B2 (en) * 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
US8055034B2 (en) * 2006-09-13 2011-11-08 Fluidigm Corporation Methods and systems for image processing of microfluidic devices
US8473216B2 (en) * 2006-11-30 2013-06-25 Fluidigm Corporation Method and program for performing baseline correction of amplification curves in a PCR experiment
EP2125219B1 (en) * 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
US7974380B2 (en) * 2007-05-09 2011-07-05 Fluidigm Corporation Method and system for crystallization and X-ray diffraction screening
WO2009033178A1 (en) 2007-09-07 2009-03-12 Fluidigm Corporation Copy number variation determination, methods and systems
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
CA3069082C (en) * 2008-09-20 2022-03-22 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8058630B2 (en) * 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods
AU2010206843B2 (en) * 2009-01-20 2015-01-29 The Board Of Trustees Of The Leland Stanford Junior University Single cell gene expression for diagnosis, prognosis and identification of drug targets

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070259351A1 (en) 2006-05-03 2007-11-08 James Chinitz Evaluating Genetic Disorders

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Vogelstein and Kinzler, 1999, Proc Natl Acad Sci USA 96:9236-41

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