KR101415660B1 - 샘플 중의 1 이상의 불순물로부터 표적 단백질을 정제하는 방법 - Google Patents

샘플 중의 1 이상의 불순물로부터 표적 단백질을 정제하는 방법 Download PDF

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KR101415660B1
KR101415660B1 KR1020127001161A KR20127001161A KR101415660B1 KR 101415660 B1 KR101415660 B1 KR 101415660B1 KR 1020127001161 A KR1020127001161 A KR 1020127001161A KR 20127001161 A KR20127001161 A KR 20127001161A KR 101415660 B1 KR101415660 B1 KR 101415660B1
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protein
buffer
column
cation exchange
region
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KR20120089432A (ko
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닐 소이스
다나 허버드
유 장
제임스 함지크
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이엠디 밀리포어 코포레이션
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
KR1020127001161A 2009-08-07 2010-08-05 샘플 중의 1 이상의 불순물로부터 표적 단백질을 정제하는 방법 Expired - Fee Related KR101415660B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27370909P 2009-08-07 2009-08-07
US61/273,709 2009-08-07
PCT/US2010/044539 WO2011017514A1 (en) 2009-08-07 2010-08-05 Methods for purifying a target protein from one or more impurities in a sample

Publications (2)

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KR20120089432A KR20120089432A (ko) 2012-08-10
KR101415660B1 true KR101415660B1 (ko) 2014-07-16

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US (1) US8536316B2 (https=)
EP (1) EP2462157B1 (https=)
JP (2) JP5791602B2 (https=)
KR (1) KR101415660B1 (https=)
CN (2) CN102574911B (https=)
ES (1) ES2817802T3 (https=)
IN (1) IN2012DN00338A (https=)
SG (1) SG177577A1 (https=)
WO (1) WO2011017514A1 (https=)

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JPWO2015041218A1 (ja) * 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）
WO2015088677A1 (en) 2013-12-12 2015-06-18 Emd Millipore Corporation Protein separations using an acrylamide containing filter
JP6612242B2 (ja) 2014-02-14 2019-11-27 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 自動化された多段階精製システム及び多段階クロマトグラフィー精製方法
ES2784749T3 (es) * 2014-03-10 2020-09-30 Richter Gedeon Nyrt Purificación de inmunoglobulina con el uso de etapas de limpieza previa
CN104826617B (zh) * 2014-03-25 2017-06-13 西北大学 一种用于蛋白质分离的wax/hic双功能混合模式色谱固定相及其制备方法
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
WO2015198320A1 (en) * 2014-06-24 2015-12-30 Insight Biopharmaceuticals Ltd. Methods of purifying antibodies
DE102014010353B4 (de) 2014-07-10 2024-09-12 Sartorius Stedim Biotech Gmbh Vorrichtung, Verwendung dieser Vorrichtung und Verfahren für die Stofftrennung mit verbesserter Ausnutzung der Kapazität chromatographischer Medien
US20170298091A1 (en) 2014-12-08 2017-10-19 Emd Millipore Corporation Mixed Bed Ion Exchange Adsorber
CN107921407B (zh) 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
EP3440093B1 (en) * 2016-04-05 2024-11-13 Haemonetics Corporation Methods and devices for the enrichment of immunoglobulin from blood
CN107964044B (zh) * 2016-10-19 2021-11-19 无锡科捷诺生物科技有限责任公司 从乳样中纯化抗cd20单克隆抗体的方法
CN111902720B (zh) 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
US20210380638A1 (en) * 2018-12-21 2021-12-09 WuXi Biologics Ireland Limited A method for improving aggregate removal by Protein A chromatography
FR3099066B1 (fr) 2019-07-26 2021-08-13 Novasep Process Procédé de purification d’une substance cible avec inactivation virale
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
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CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法
CN113845562A (zh) * 2020-06-28 2021-12-28 佛山汉腾生物科技有限公司 目的蛋白纯化方法

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EP2462157B1 (en) 2020-06-17
US8536316B2 (en) 2013-09-17
JP2015157846A (ja) 2015-09-03
JP2013501721A (ja) 2013-01-17
EP2462157A4 (en) 2012-12-26
SG177577A1 (en) 2012-03-29
KR20120089432A (ko) 2012-08-10
EP2462157A1 (en) 2012-06-13
IN2012DN00338A (https=) 2015-05-22
CN102574911A (zh) 2012-07-11
ES2817802T3 (es) 2021-04-08
JP5791602B2 (ja) 2015-10-07
WO2011017514A1 (en) 2011-02-10
JP6335838B2 (ja) 2018-05-30
CN107188960A (zh) 2017-09-22
CN102574911B (zh) 2017-06-06
US20110065901A1 (en) 2011-03-17

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