CN107188960A - 从样品的一或多种杂质中纯化靶蛋白的方法 - Google Patents

从样品的一或多种杂质中纯化靶蛋白的方法 Download PDF

Info

Publication number
CN107188960A
CN107188960A CN201710418825.9A CN201710418825A CN107188960A CN 107188960 A CN107188960 A CN 107188960A CN 201710418825 A CN201710418825 A CN 201710418825A CN 107188960 A CN107188960 A CN 107188960A
Authority
CN
China
Prior art keywords
protein
buffer
column
cation exchange
resin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710418825.9A
Other languages
English (en)
Chinese (zh)
Inventor
N·索伊斯
J·D·哈伯德
Y·张
J·海姆齐克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Millipore Corp filed Critical Millipore Corp
Publication of CN107188960A publication Critical patent/CN107188960A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
CN201710418825.9A 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法 Pending CN107188960A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27370909P 2009-08-07 2009-08-07
US61/273,709 2009-08-07
CN201080035153.XA CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201080035153.XA Division CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Publications (1)

Publication Number Publication Date
CN107188960A true CN107188960A (zh) 2017-09-22

Family

ID=43544660

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201710418825.9A Pending CN107188960A (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法
CN201080035153.XA Expired - Fee Related CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201080035153.XA Expired - Fee Related CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Country Status (9)

Country Link
US (1) US8536316B2 (https=)
EP (1) EP2462157B1 (https=)
JP (2) JP5791602B2 (https=)
KR (1) KR101415660B1 (https=)
CN (2) CN107188960A (https=)
ES (1) ES2817802T3 (https=)
IN (1) IN2012DN00338A (https=)
SG (1) SG177577A1 (https=)
WO (1) WO2011017514A1 (https=)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3237179B2 (ja) 1992-03-18 2001-12-10 栗田工業株式会社 排超純水中の過酸化水素の除去方法
EP2519536A4 (en) * 2009-12-29 2013-06-05 Reddys Lab Ltd Dr PROTEIN PURIFICATION BY ION EXCHANGE
KR101976853B1 (ko) 2010-05-25 2019-05-09 제넨테크, 인크. 폴리펩티드의 정제 방법
KR101619865B1 (ko) 2010-07-30 2016-05-11 이엠디 밀리포어 코포레이션 크로마토그래피 매질
JP6055615B2 (ja) 2011-05-27 2016-12-27 アッヴィ バイオテクノロジー リミテッド Ｄａｃｈｙｐ組成物および方法
US20140187749A1 (en) * 2011-06-16 2014-07-03 Dsm Ip Assets B.V. Single unit chromatography antibody purification
DK2729482T3 (en) 2011-07-08 2018-05-07 Merck Sharp & Dohme PROCEDURE FOR CLEANING FC-FUSION PROTEIN
CN103827134A (zh) * 2011-07-20 2014-05-28 泽普泰恩股份有限公司 多肽分离方法
CN103917555A (zh) * 2011-08-01 2014-07-09 艾瓦夏生物制剂公司 人tnf特异性的牛科动物多克隆抗体
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
WO2013033517A1 (en) * 2011-09-01 2013-03-07 Human Genome Sciences, Inc. Viral clearance methods
EP2578286A1 (en) 2011-10-04 2013-04-10 Merck Patent GmbH Method and apparatus for chromatographic purification
HUE035685T2 (en) * 2011-11-02 2018-05-28 Hoffmann La Roche Overload and elute chromatography
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
CN102492040B (zh) * 2011-12-29 2014-06-25 嘉和生物药业有限公司 一种抗her2或/和抗her3抗体蛋白的纯化方法
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
JP6253584B2 (ja) * 2012-08-27 2017-12-27 旭化成メディカル株式会社 温度応答性クロマトグラフィーによる抗体の精製方法
SI2895188T1 (en) * 2012-09-11 2018-05-31 Coherus Biosciences, Inc. CORRECTED QUALITY ETHANERCEPT WITH HIGH QUALITY AND EXCELLENT EXPERIENCE
EP2812091B1 (en) 2012-09-17 2021-03-10 W.R. Grace & CO. - CONN. Chromatography media and devices
US10421795B2 (en) 2012-12-17 2019-09-24 Merck Sharp & Dohme Corp. Process for purifying insulin and analogues thereof
EP2959509B1 (en) 2013-02-14 2018-05-23 Nanopareil, Llc Electrospun hybrid nanofibre felt, method for making the same, and method for purifying biomolecules
EP3301115B1 (en) * 2013-05-06 2021-11-10 Sanofi Continuous multistep process for purifying antibodies
US20150093800A1 (en) * 2013-09-05 2015-04-02 Genentech, Inc. Method for chromatography reuse
JPWO2015041218A1 (ja) * 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）
JP6326499B2 (ja) 2013-12-12 2018-05-16 イー・エム・デイー・ミリポア・コーポレイシヨン アクリルアミド含有フィルターを使用したタンパク質分離
JP6612242B2 (ja) * 2014-02-14 2019-11-27 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 自動化された多段階精製システム及び多段階クロマトグラフィー精製方法
PL3116891T3 (pl) 2014-03-10 2020-07-27 Richter Gedeon Nyrt. Oczyszczanie immunoglobuliny z zastosowaniem etapów wstępnego oczyszczania
CN104826617B (zh) * 2014-03-25 2017-06-13 西北大学 一种用于蛋白质分离的wax/hic双功能混合模式色谱固定相及其制备方法
PL3137209T3 (pl) 2014-05-02 2023-01-02 W.R. Grace & Co. - Conn. Funkcjonalizowany materiał nośnikowy i sposoby wytwarzania oraz stosowania funkcjonalizowanego materiału nośnikowego
WO2015198320A1 (en) * 2014-06-24 2015-12-30 Insight Biopharmaceuticals Ltd. Methods of purifying antibodies
DE102014010353B4 (de) 2014-07-10 2024-09-12 Sartorius Stedim Biotech Gmbh Vorrichtung, Verwendung dieser Vorrichtung und Verfahren für die Stofftrennung mit verbesserter Ausnutzung der Kapazität chromatographischer Medien
EP3230299A1 (en) 2014-12-08 2017-10-18 EMD Millipore Corporation Mixed bed ion exchange adsorber
KR102566292B1 (ko) 2015-06-05 2023-08-10 더블유.알. 그레이스 앤드 캄파니-콘. 흡착성 바이오프로세싱 정화제와 이의 제조 및 사용 방법
JP7163187B2 (ja) * 2016-04-05 2022-10-31 ヘモネティクス・コーポレーション 血液からの免疫グロブリンの濃縮のための方法および装置
CN107964044B (zh) * 2016-10-19 2021-11-19 无锡科捷诺生物科技有限责任公司 从乳样中纯化抗cd20单克隆抗体的方法
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
WO2020125757A1 (en) * 2018-12-21 2020-06-25 Wuxi Biologics (Shanghai) Co., Ltd. A method for improving aggregate removal by protein a chromatography
FR3099066B1 (fr) 2019-07-26 2021-08-13 Novasep Process Procédé de purification d’une substance cible avec inactivation virale
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
CA3156649A1 (en) * 2019-10-04 2021-04-08 Merck Patent Gmbh PROTEIN PURIFICATION AND VIRAL INACTIVATION
CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法
CN113845562A (zh) * 2020-06-28 2021-12-28 佛山汉腾生物科技有限公司 目的蛋白纯化方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008025748A1 (en) * 2006-08-28 2008-03-06 Ares Trading S.A. Process for the purification of fc-containing proteins
WO2008025747A1 (en) * 2006-08-28 2008-03-06 Ares Trading S.A. Process for the purification of fc-fusion proteins
WO2009017491A1 (en) * 2006-06-14 2009-02-05 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4490937A (en) 1981-12-07 1985-01-01 Amcor Ltd. Insect electrocution device
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5256694A (en) 1984-09-22 1993-10-26 Basf Aktiengesellschaft Diarylacetylenes, their preparation and their use
US5091178A (en) 1986-02-21 1992-02-25 Oncogen Tumor therapy with biologically active anti-tumor antibodies
US6054051A (en) 1996-01-17 2000-04-25 Genentech, Inc. Tangential-flow filtration system
WO2003102132A2 (en) 2002-04-26 2003-12-11 Genetech, Inc. Non-affinity purification of proteins
EP3225625A1 (en) * 2002-09-11 2017-10-04 Chugai Seiyaku Kabushiki Kaisha Protein purification method
GB0304576D0 (en) 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
CA2581208A1 (en) * 2004-08-30 2006-03-09 Lonza Biologics Plc. Affinity- plus ion exchange- chromatography for purifying antibodies
AU2008206923A1 (en) * 2007-01-17 2008-07-24 Merck Serono S.A. Process for the purification of Fc-containing proteins
US9433922B2 (en) 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
WO2009045897A1 (en) 2007-10-03 2009-04-09 Dyax Corp. Systems and methods for purifying proteins
EP2281000A2 (en) 2008-05-15 2011-02-09 Novo Nordisk A/S Antibody purification process
BRPI0920572A8 (pt) * 2008-10-20 2015-10-27 Abbott Lab Inativação viral durante a purificação dos anticorpos

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009017491A1 (en) * 2006-06-14 2009-02-05 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
WO2008025748A1 (en) * 2006-08-28 2008-03-06 Ares Trading S.A. Process for the purification of fc-containing proteins
WO2008025747A1 (en) * 2006-08-28 2008-03-06 Ares Trading S.A. Process for the purification of fc-fusion proteins

Also Published As

Publication number Publication date
IN2012DN00338A (https=) 2015-05-22
JP6335838B2 (ja) 2018-05-30
SG177577A1 (en) 2012-03-29
EP2462157A1 (en) 2012-06-13
KR101415660B1 (ko) 2014-07-16
CN102574911A (zh) 2012-07-11
CN102574911B (zh) 2017-06-06
WO2011017514A1 (en) 2011-02-10
US8536316B2 (en) 2013-09-17
ES2817802T3 (es) 2021-04-08
JP5791602B2 (ja) 2015-10-07
JP2015157846A (ja) 2015-09-03
US20110065901A1 (en) 2011-03-17
KR20120089432A (ko) 2012-08-10
EP2462157A4 (en) 2012-12-26
EP2462157B1 (en) 2020-06-17
JP2013501721A (ja) 2013-01-17

Similar Documents

Publication Publication Date Title
CN102574911B (zh) 从样品的一或多种杂质中纯化靶蛋白的方法
US11590434B2 (en) Method and apparatus for chromatographic purification
US10865224B2 (en) Purification of biological molecules
CN102977182B (zh) 在蛋白质纯化过程中降低样品中的一种或多种杂质水平的方法
US8697847B2 (en) Protein purification using HCIC and ion exchange chromatography
CN101437839A (zh) 蛋白质纯化
AU2012269240B2 (en) Single unit chromatography antibody purification

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170922

RJ01 Rejection of invention patent application after publication