SG177577A1 - Methods for purifying a target protein from one or more impurities in a sample - Google Patents

Methods for purifying a target protein from one or more impurities in a sample Download PDF

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Publication number
SG177577A1
SG177577A1 SG2012001483A SG2012001483A SG177577A1 SG 177577 A1 SG177577 A1 SG 177577A1 SG 2012001483 A SG2012001483 A SG 2012001483A SG 2012001483 A SG2012001483 A SG 2012001483A SG 177577 A1 SG177577 A1 SG 177577A1
Authority
SG
Singapore
Prior art keywords
protein
buffer
target protein
region containing
cation exchange
Prior art date
Application number
SG2012001483A
Other languages
English (en)
Inventor
Neil Soice
Dana Hubbard
Yu Zhang
James Harmzik
Original Assignee
Emd Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emd Millipore Corp filed Critical Emd Millipore Corp
Publication of SG177577A1 publication Critical patent/SG177577A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
SG2012001483A 2009-08-07 2010-08-05 Methods for purifying a target protein from one or more impurities in a sample SG177577A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27370909P 2009-08-07 2009-08-07
PCT/US2010/044539 WO2011017514A1 (en) 2009-08-07 2010-08-05 Methods for purifying a target protein from one or more impurities in a sample

Publications (1)

Publication Number Publication Date
SG177577A1 true SG177577A1 (en) 2012-03-29

Family

ID=43544660

Family Applications (1)

Application Number Title Priority Date Filing Date
SG2012001483A SG177577A1 (en) 2009-08-07 2010-08-05 Methods for purifying a target protein from one or more impurities in a sample

Country Status (9)

Country Link
US (1) US8536316B2 (https=)
EP (1) EP2462157B1 (https=)
JP (2) JP5791602B2 (https=)
KR (1) KR101415660B1 (https=)
CN (2) CN102574911B (https=)
ES (1) ES2817802T3 (https=)
IN (1) IN2012DN00338A (https=)
SG (1) SG177577A1 (https=)
WO (1) WO2011017514A1 (https=)

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CN104826617B (zh) * 2014-03-25 2017-06-13 西北大学 一种用于蛋白质分离的wax/hic双功能混合模式色谱固定相及其制备方法
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
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DE102014010353B4 (de) 2014-07-10 2024-09-12 Sartorius Stedim Biotech Gmbh Vorrichtung, Verwendung dieser Vorrichtung und Verfahren für die Stofftrennung mit verbesserter Ausnutzung der Kapazität chromatographischer Medien
US20170298091A1 (en) 2014-12-08 2017-10-19 Emd Millipore Corporation Mixed Bed Ion Exchange Adsorber
CN107921407B (zh) 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
EP3440093B1 (en) * 2016-04-05 2024-11-13 Haemonetics Corporation Methods and devices for the enrichment of immunoglobulin from blood
CN107964044B (zh) * 2016-10-19 2021-11-19 无锡科捷诺生物科技有限责任公司 从乳样中纯化抗cd20单克隆抗体的方法
CN111902720B (zh) 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
US20210380638A1 (en) * 2018-12-21 2021-12-09 WuXi Biologics Ireland Limited A method for improving aggregate removal by Protein A chromatography
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Also Published As

Publication number Publication date
EP2462157B1 (en) 2020-06-17
US8536316B2 (en) 2013-09-17
JP2015157846A (ja) 2015-09-03
JP2013501721A (ja) 2013-01-17
EP2462157A4 (en) 2012-12-26
KR101415660B1 (ko) 2014-07-16
KR20120089432A (ko) 2012-08-10
EP2462157A1 (en) 2012-06-13
IN2012DN00338A (https=) 2015-05-22
CN102574911A (zh) 2012-07-11
ES2817802T3 (es) 2021-04-08
JP5791602B2 (ja) 2015-10-07
WO2011017514A1 (en) 2011-02-10
JP6335838B2 (ja) 2018-05-30
CN107188960A (zh) 2017-09-22
CN102574911B (zh) 2017-06-06
US20110065901A1 (en) 2011-03-17

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