KR101269656B1 - Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 - Google Patents
Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 Download PDFInfo
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- KR101269656B1 KR101269656B1 KR1020117023134A KR20117023134A KR101269656B1 KR 101269656 B1 KR101269656 B1 KR 101269656B1 KR 1020117023134 A KR1020117023134 A KR 1020117023134A KR 20117023134 A KR20117023134 A KR 20117023134A KR 101269656 B1 KR101269656 B1 KR 101269656B1
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Evolutionary Biology (AREA)
- Theoretical Computer Science (AREA)
- Medical Informatics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51357403P | 2003-10-24 | 2003-10-24 | |
| US60/513,574 | 2003-10-24 | ||
| EP04002722 | 2004-02-06 | ||
| EP04002722.9 | 2004-02-06 | ||
| PCT/EP2004/011974 WO2005040377A2 (en) | 2003-10-24 | 2004-10-22 | High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020067006395A Division KR101222025B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물세포에서의 고효율 유전자 전달 및 발현 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20110112884A KR20110112884A (ko) | 2011-10-13 |
| KR101269656B1 true KR101269656B1 (ko) | 2013-05-30 |
Family
ID=34524793
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020117023134A Expired - Lifetime KR101269656B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
| KR1020117023131A Expired - Fee Related KR101230838B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
| KR1020117023132A Expired - Fee Related KR101229989B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
| KR1020067006395A Expired - Fee Related KR101222025B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물세포에서의 고효율 유전자 전달 및 발현 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020117023131A Expired - Fee Related KR101230838B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
| KR1020117023132A Expired - Fee Related KR101229989B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
| KR1020067006395A Expired - Fee Related KR101222025B1 (ko) | 2003-10-24 | 2004-10-22 | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물세포에서의 고효율 유전자 전달 및 발현 |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US8252917B2 (enExample) |
| EP (5) | EP1959011B1 (enExample) |
| JP (1) | JP5396653B2 (enExample) |
| KR (4) | KR101269656B1 (enExample) |
| CN (1) | CN1863913B (enExample) |
| AT (1) | ATE551420T1 (enExample) |
| AU (4) | AU2004284220B2 (enExample) |
| CA (1) | CA2535836C (enExample) |
| IL (2) | IL175142A0 (enExample) |
| IN (2) | IN2014DN02484A (enExample) |
| PL (4) | PL2298898T3 (enExample) |
| RU (1) | RU2375450C2 (enExample) |
| SG (1) | SG147468A1 (enExample) |
| WO (1) | WO2005040377A2 (enExample) |
| ZA (1) | ZA200604032B (enExample) |
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| EP1572994B1 (en) | 2002-12-20 | 2007-02-21 | Chromagenics B.V. | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors |
| IN2014DN02484A (enExample) | 2003-10-24 | 2015-05-15 | Selexis Sa | |
| DE102004043207C5 (de) * | 2004-09-03 | 2010-09-09 | Südzucker AG Mannheim/Ochsenfurt | Wurzel- und xylemparenchymspezifischer Promotor |
| US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| KR101271884B1 (ko) | 2004-11-08 | 2013-06-05 | 크로마제닉스 비.브이. | 고수준으로 단백질을 발현하는 숙주세포의 선택 |
| WO2007107578A1 (en) | 2006-03-20 | 2007-09-27 | Chromagenics B.V. | Expression augmenting dna fragments, use thereof, and methods for finding thereof |
| SG176501A1 (en) * | 2006-08-23 | 2011-12-29 | Selexis Sa | Matrix attachment regions (mars) for increasing transcription and uses thereof |
| EP1975228A1 (en) | 2007-03-28 | 2008-10-01 | Fachhochschule Mannheim | Polynucleotides for enhancing expression of a polynucleotide of interest |
| WO2009079796A1 (en) | 2007-12-24 | 2009-07-02 | Id Biomedical Corporation Of Quebec | Recombinant rsv antigens |
| CN101580842B (zh) * | 2008-05-15 | 2011-02-09 | 中国农业科学院生物技术研究所 | 一种增强基因表达的dna分子及其应用 |
| EP2379598B1 (en) | 2009-01-19 | 2015-03-11 | Innate Pharma | Anti-kir3d antibodies |
| AU2010264688A1 (en) | 2009-06-24 | 2012-01-19 | Glaxosmithkline Biologicals S.A. | Recombinant RSV antigens |
| WO2010149743A2 (en) | 2009-06-24 | 2010-12-29 | Id Biomedical Corporation Of Quebec | Vaccine |
| SI3178490T1 (sl) | 2009-07-15 | 2022-08-31 | Glaxosmithkline Biologicals S.A. | Sestavki proteina F RSV-ja in postopki za pripravo le-teh |
| JP2013505013A (ja) * | 2009-09-18 | 2013-02-14 | セレクシス エス.エー. | 強化導入遺伝子発現およびプロセッシングの産物および方法 |
| WO2011070405A1 (en) * | 2009-12-07 | 2011-06-16 | Avesthagen Limited | An expression vector carrying scaffold/matrix attachment region(s) |
| RU2422512C1 (ru) * | 2010-02-16 | 2011-06-27 | Автономная некоммерческая организация "Институт молекулярной диагностики" (АНО "ИнМоДи") | ШТАММ Escherichia coli BL21(DE3)/pAFP11D3 - ПРОДУЦЕНТ ФРАГМЕНТА С 404 ПО 609 АМИНОКИСЛОТУ АЛЬФА-ФЕТОПРОТЕИНА ЧЕЛОВЕКА |
| WO2012019630A1 (en) | 2010-08-13 | 2012-02-16 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded protein |
| WO2012095514A1 (en) | 2011-01-14 | 2012-07-19 | Vivalis | Recombinant protein production system |
| WO2012139195A1 (en) | 2011-04-13 | 2012-10-18 | National Research Council Of Canada | EXPRESSION SYSTEM WITH SAR ELEMENT FROM IFNα2 |
| SI3275892T1 (sl) | 2011-05-13 | 2020-06-30 | Glaxosmithkline Biologicals S.A. | Prefuzijski RSV F antigeni |
| WO2013120498A1 (en) | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded allergenic antigen or an autoimmune self-antigen |
| WO2013120500A1 (en) | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded tumour antigen |
| WO2013120499A1 (en) | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly (a) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen |
| WO2013120497A1 (en) | 2012-02-15 | 2013-08-22 | Curevac Gmbh | Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded therapeutic protein |
| ES2969742T3 (es) | 2012-03-27 | 2024-05-22 | CureVac SE | Moléculas artificiales de ácido nucleico para la expresión mejorada de proteínas o péptidos |
| ES2717175T3 (es) | 2013-02-01 | 2019-06-19 | Selexis Sa | Expresión mejorada de transgenes y procesamiento |
| CN103667440A (zh) * | 2013-09-05 | 2014-03-26 | 谢小冬 | Gata4基因snp位点的用途 |
| US9982027B2 (en) | 2013-10-22 | 2018-05-29 | Lubris Llc | Control of rheological properties of mixed hyaluronate/lubricin solutions |
| CN109576265B (zh) * | 2014-04-01 | 2020-08-28 | 三生国健药业(上海)股份有限公司 | 一种含mar核心片段的动物细胞表达载体 |
| CN104974933B (zh) * | 2014-04-04 | 2017-08-15 | 上海泰因生物技术有限公司 | 一种大规模连续多次悬浮瞬转表达重组蛋白的装置和方法 |
| WO2017202776A1 (en) * | 2016-05-23 | 2017-11-30 | Luxembourg Institute Of Health (Lih) | Multifunctional heteromultimeric constructs. |
| DK3456821T4 (da) * | 2017-09-19 | 2024-04-15 | Deutsches Krebsforsch | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
| WO2020084528A1 (en) | 2018-10-24 | 2020-04-30 | Selexis Sa | Expression systems, recombinant cells and uses thereof |
| CN113383081B (zh) * | 2018-12-07 | 2025-04-01 | 赛尔希恩公司 | 基质附着区和促进基因表达的用途 |
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