JP5396653B2 - Mar配列の多トランスフェクション手順による高効率の遺伝子導入および哺乳動物細胞における発現 - Google Patents

Mar配列の多トランスフェクション手順による高効率の遺伝子導入および哺乳動物細胞における発現 Download PDF

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JP5396653B2
JP5396653B2 JP2006536060A JP2006536060A JP5396653B2 JP 5396653 B2 JP5396653 B2 JP 5396653B2 JP 2006536060 A JP2006536060 A JP 2006536060A JP 2006536060 A JP2006536060 A JP 2006536060A JP 5396653 B2 JP5396653 B2 JP 5396653B2
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sequence
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sequences
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JP2007508831A (ja
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メルモ、ニコラ
アラン ジロ、ピエール
ブシェール、フィリップ
ヌエン、デュク−クァング
カラブレセ、ダヴィ
ソジュ、ダミアン
プティーニ、ステファニア
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セレキス エスアー
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    • C12N15/09Recombinant DNA-technology
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JP2006536060A 2003-10-24 2004-10-22 Mar配列の多トランスフェクション手順による高効率の遺伝子導入および哺乳動物細胞における発現 Expired - Lifetime JP5396653B2 (ja)

Applications Claiming Priority (5)

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US51357403P 2003-10-24 2003-10-24
US60/513,574 2003-10-24
EP04002722 2004-02-06
EP04002722.9 2004-02-06
PCT/EP2004/011974 WO2005040377A2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences

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JP5396653B2 true JP5396653B2 (ja) 2014-01-22

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US (3) US8252917B2 (enExample)
EP (5) EP1959011B1 (enExample)
JP (1) JP5396653B2 (enExample)
KR (4) KR101269656B1 (enExample)
CN (1) CN1863913B (enExample)
AT (1) ATE551420T1 (enExample)
AU (4) AU2004284220B2 (enExample)
CA (1) CA2535836C (enExample)
IL (2) IL175142A0 (enExample)
IN (2) IN2014DN02484A (enExample)
PL (4) PL2298898T3 (enExample)
RU (1) RU2375450C2 (enExample)
SG (1) SG147468A1 (enExample)
WO (1) WO2005040377A2 (enExample)
ZA (1) ZA200604032B (enExample)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1572994B1 (en) 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
IN2014DN02484A (enExample) 2003-10-24 2015-05-15 Selexis Sa
DE102004043207C5 (de) * 2004-09-03 2010-09-09 Südzucker AG Mannheim/Ochsenfurt Wurzel- und xylemparenchymspezifischer Promotor
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
KR101271884B1 (ko) 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
WO2007107578A1 (en) 2006-03-20 2007-09-27 Chromagenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
SG176501A1 (en) * 2006-08-23 2011-12-29 Selexis Sa Matrix attachment regions (mars) for increasing transcription and uses thereof
EP1975228A1 (en) 2007-03-28 2008-10-01 Fachhochschule Mannheim Polynucleotides for enhancing expression of a polynucleotide of interest
WO2009079796A1 (en) 2007-12-24 2009-07-02 Id Biomedical Corporation Of Quebec Recombinant rsv antigens
CN101580842B (zh) * 2008-05-15 2011-02-09 中国农业科学院生物技术研究所 一种增强基因表达的dna分子及其应用
EP2379598B1 (en) 2009-01-19 2015-03-11 Innate Pharma Anti-kir3d antibodies
AU2010264688A1 (en) 2009-06-24 2012-01-19 Glaxosmithkline Biologicals S.A. Recombinant RSV antigens
WO2010149743A2 (en) 2009-06-24 2010-12-29 Id Biomedical Corporation Of Quebec Vaccine
SI3178490T1 (sl) 2009-07-15 2022-08-31 Glaxosmithkline Biologicals S.A. Sestavki proteina F RSV-ja in postopki za pripravo le-teh
JP2013505013A (ja) * 2009-09-18 2013-02-14 セレクシス エス.エー. 強化導入遺伝子発現およびプロセッシングの産物および方法
WO2011070405A1 (en) * 2009-12-07 2011-06-16 Avesthagen Limited An expression vector carrying scaffold/matrix attachment region(s)
RU2422512C1 (ru) * 2010-02-16 2011-06-27 Автономная некоммерческая организация "Институт молекулярной диагностики" (АНО "ИнМоДи") ШТАММ Escherichia coli BL21(DE3)/pAFP11D3 - ПРОДУЦЕНТ ФРАГМЕНТА С 404 ПО 609 АМИНОКИСЛОТУ АЛЬФА-ФЕТОПРОТЕИНА ЧЕЛОВЕКА
WO2012019630A1 (en) 2010-08-13 2012-02-16 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded protein
WO2012095514A1 (en) 2011-01-14 2012-07-19 Vivalis Recombinant protein production system
WO2012139195A1 (en) 2011-04-13 2012-10-18 National Research Council Of Canada EXPRESSION SYSTEM WITH SAR ELEMENT FROM IFNα2
SI3275892T1 (sl) 2011-05-13 2020-06-30 Glaxosmithkline Biologicals S.A. Prefuzijski RSV F antigeni
WO2013120498A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded allergenic antigen or an autoimmune self-antigen
WO2013120500A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded tumour antigen
WO2013120499A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly (a) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen
WO2013120497A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded therapeutic protein
ES2969742T3 (es) 2012-03-27 2024-05-22 CureVac SE Moléculas artificiales de ácido nucleico para la expresión mejorada de proteínas o péptidos
ES2717175T3 (es) 2013-02-01 2019-06-19 Selexis Sa Expresión mejorada de transgenes y procesamiento
CN103667440A (zh) * 2013-09-05 2014-03-26 谢小冬 Gata4基因snp位点的用途
US9982027B2 (en) 2013-10-22 2018-05-29 Lubris Llc Control of rheological properties of mixed hyaluronate/lubricin solutions
CN109576265B (zh) * 2014-04-01 2020-08-28 三生国健药业(上海)股份有限公司 一种含mar核心片段的动物细胞表达载体
CN104974933B (zh) * 2014-04-04 2017-08-15 上海泰因生物技术有限公司 一种大规模连续多次悬浮瞬转表达重组蛋白的装置和方法
WO2017202776A1 (en) * 2016-05-23 2017-11-30 Luxembourg Institute Of Health (Lih) Multifunctional heteromultimeric constructs.
DK3456821T4 (da) * 2017-09-19 2024-04-15 Deutsches Krebsforsch Ikke-integrerende dna-vektorer til genetisk modifikation af celler
WO2020084528A1 (en) 2018-10-24 2020-04-30 Selexis Sa Expression systems, recombinant cells and uses thereof
CN113383081B (zh) * 2018-12-07 2025-04-01 赛尔希恩公司 基质附着区和促进基因表达的用途
JP2023515504A (ja) 2020-02-24 2023-04-13 ノバルティス アーゲー 組み換え産生されたポリペプチドの精製
AU2020447192A1 (en) * 2020-05-11 2023-01-19 Samsung Bioepis Co., Ltd. Human genome-derived polynucleotide and method for producing polypeptide of interest using same
CN116209767A (zh) * 2020-07-22 2023-06-02 德国癌症研究中心 5’s/mar应用

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1077994A (en) * 1963-04-18 1967-08-02 Kobe Steel Ltd Process for producing cold-forged products from tempered steel wire
DE2605560C3 (de) 1976-02-12 1979-08-30 Siemens Ag, 1000 Berlin Und 8000 Muenchen Aufschlußverfahren für Biomaterialien
DE3376405D1 (en) 1982-12-23 1988-06-01 Britax Weathershields Vehicle sliding roof
JP2874751B2 (ja) 1986-04-09 1999-03-24 ジェンザイム・コーポレーション 希望する蛋白質をミルク中へ分泌する遺伝子移植動物
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
GB8925590D0 (en) 1989-11-13 1990-01-04 Central Blood Lab Authority Monoclonal antibodies
US5968502A (en) 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6063630A (en) 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
PT663921E (pt) 1992-10-05 2002-10-31 Univ North Carolina State Metodo para aumentar a expressao e reduzir a variabilidade de expressao de genes estranhos em celulas vegetais
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5888981A (en) 1993-06-14 1999-03-30 Basf Aktiengesellschaft Methods for regulating gene expression
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5907078A (en) 1994-12-09 1999-05-25 Greenberg; Norman M. Transgenic mouse model for prostate cancer
US5705364A (en) 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
DE19541450C2 (de) 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Genkonstrukt und dessen Verwendung
US6596514B2 (en) 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
WO1997032018A2 (en) 1996-02-29 1997-09-04 Immusol, Inc. Hepatitis c virus ribozymes
EP0914444A1 (en) 1996-06-06 1999-05-12 Novartis AG Vectors comprising sar elements
CA2689842A1 (en) 1996-12-02 1998-06-11 Genaera Corporation Il-9 receptor variants, useful in treating and diagnosing atopic allergies including asthma and related disorders
US6245974B1 (en) 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
EP1029048A2 (en) 1997-11-05 2000-08-23 Baylor College Of Medicine Sequences for targeting metastatic cells
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6004284A (en) 1998-06-04 1999-12-21 Alcon Laboratories, Inc. Surgical handpiece
WO2000005393A2 (en) 1998-07-21 2000-02-03 Cobra Therapeutics Limited A polynucleotide comprising a ubiquitous chromatin opening element (ucoe)
US6338066B1 (en) 1998-09-25 2002-01-08 International Business Machines Corporation Surfaid predictor: web-based system for predicting surfer behavior
WO2000020950A1 (en) 1998-10-07 2000-04-13 Adobe Systems, Inc. Distributing access to a data item
DE19848017A1 (de) 1998-10-17 2000-04-20 Multigene Biotech Gmbh Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen
ATE279524T1 (de) 1998-12-01 2004-10-15 Dow Agrosciences Llc Künstliche matrix-anheftungsregion zur erhöhung der expression von in pflanzen eingeführten genen
US6225113B1 (en) 1998-12-04 2001-05-01 Genvec, Inc. Use of trans-activation and cis-activation to modulate the persistence of expression of a transgene in an at least E4-deficient adenovirus
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
EP1181359A1 (en) 1999-05-28 2002-02-27 Sangamo Biosciences Inc. Gene switches
US20050129669A1 (en) 1999-06-21 2005-06-16 Transkaryotic Therapies, Inc., A Massachusetts Corporation DNA construct for effecting homologous recombination and uses thereof
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
JP3507858B2 (ja) 1999-11-19 2004-03-15 奈良先端科学技術大学院大学長 植物において外来遺伝子の発現を安定化させるdna断片、外来遺伝子を植物において安定に発現させる遺伝子導入方法、外来遺伝子を安定に発現させた形質転換植物
WO2001040488A1 (en) 1999-11-30 2001-06-07 Novartis Ag Increased transgene expression in retroviral vectors having scaffold attachment region
US6864085B2 (en) 1999-12-14 2005-03-08 Novartis Ag Bovine immunodeficiency virus (BIV) based vectors
US20020127199A1 (en) 2000-02-03 2002-09-12 Tang Y. Tom Novel nucleic acids and polypeptides
US6821775B1 (en) 2000-02-11 2004-11-23 Genvec, Inc. Viral vector encoding pigment epithelium-derived factor
DE10006886A1 (de) 2000-02-16 2001-08-23 Hepavec Ag Fuer Gentherapie Nichthumaner helferabhängiger Virusvektor
US6569681B1 (en) 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
US6747189B1 (en) 2000-03-21 2004-06-08 Dekalb Genetics Corporation Maize glycine rich protein promoter compositions and methods for use thereof
US6730826B2 (en) 2000-03-22 2004-05-04 University Of Kentucky Research Foundation Plant trichome gland specific promoter sequence
JP2004501620A (ja) 2000-06-26 2004-01-22 ユニヴェルシテ・ドゥ・ジュネーブ クロマチンリモデリング物質による染色体機能の調整
US20030157715A1 (en) 2000-06-26 2003-08-21 Ulrich Laemmli Modulation of chromosome function by chromatin remodeling agents
CA2416877C (en) 2000-07-27 2013-03-12 Apogene Gmbh & Co. Kg Somatic cloning gene transfer for producing recombinant proteins, cells and organs
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
KR20030029885A (ko) 2000-08-30 2003-04-16 노쓰 캐롤라이나 스테이트 유니버시티 단백질 함량을 변화시키는 분자 미끼를 포함하는유전자이식 식물
US20050034187A1 (en) 2000-09-09 2005-02-10 Andrei Golovko Modified tet-inducible system for regulation of gene expression in plants
GB0022995D0 (en) 2000-09-20 2000-11-01 Cobra Therapeutics Ltd Polynucleotide
US6919204B2 (en) 2000-09-29 2005-07-19 Sangamo Biosciences, Inc. Modulation of gene expression using localization domains
AU2002241556B2 (en) 2000-11-01 2007-07-19 Elusys Therapeutics, Inc. Method of producing bispecific molecules by protein trans-splicing
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
US7259010B2 (en) 2000-12-15 2007-08-21 Pangen Biotech Inc. Expression vector for animal cell containing nuclear matrix attachment region of interferon beta
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
ATE437233T1 (de) 2001-01-26 2009-08-15 Selexis Sa Matrix-anheftungsregionen und verfahren zu deren verwendung
DE10109780A1 (de) 2001-02-28 2002-09-05 Cardion Ag Episomale Vektoren enthaltend Matrix-Anheftungsregionen zur zellspezifischen Genexpression
WO2002072138A1 (en) 2001-03-08 2002-09-19 Hyseq, Inc. Methods and materials relating to fibulin-like polypeptides and polynucleotides
JP4817514B2 (ja) 2001-03-09 2011-11-16 協和発酵キリン株式会社 新規動物細胞用ベクターおよびその使用
WO2002072789A2 (en) 2001-03-12 2002-09-19 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
NZ527937A (en) 2001-03-13 2005-03-24 Novartis Ag Stable cell lines to express high levels of lentiviral Gag/Pol proteins for generation of HIV- or BIV- based lentiviral vectors
EP1478751A4 (en) 2001-03-30 2005-10-19 Avigenics Inc LYSOZYM PROMOTER IN BIRDS
DE10129010A1 (de) 2001-06-13 2002-12-19 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
US20030032597A1 (en) 2001-07-31 2003-02-13 Sebestyen Magdolna G. Targeting nucleic acids to a cellular nucleus
KR100464962B1 (ko) * 2001-09-14 2005-01-05 삼화강봉주식회사 냉간압조 특성이 우수한 조질 강선
US7122715B2 (en) 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
WO2003024199A2 (en) 2001-09-21 2003-03-27 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US7138278B2 (en) 2001-11-20 2006-11-21 Monsanto Technology, L.L.C. Maize cytoplasmic glutamine synthetase promoter compositions and methods for use thereof
FR2832423B1 (fr) 2001-11-22 2004-10-08 Vivalis Systeme d'expression de proteines exogenes dans un systeme aviaire
US6875588B2 (en) 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use
CA2427190A1 (en) 2002-04-30 2003-10-30 Alberta Research Council Inc. Production of recombinant epidermal growth factor in plants
US20030224477A1 (en) 2002-05-31 2003-12-04 Heartlein Michael W. Optimized promoter constructs
AU2003267967B2 (en) 2002-06-18 2008-11-20 Zymogenetics, Inc. Hybrid vector having a cytomegalovirus enhancer and myeloproliferative sarcoma virus promoter
US20040016015A1 (en) 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US6933136B2 (en) 2002-09-20 2005-08-23 Novo Nordisk A/S Method for making recombinant proteins
CN1263860C (zh) 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
WO2004053106A2 (en) 2002-12-05 2004-06-24 Regulome Corporation Profiled regulatory sites useful for gene control
AU2003283174A1 (en) 2002-12-11 2004-06-30 Cytos Biotechnology Ag Method for protein production
AU2002953381A0 (en) 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
EP1445320A1 (en) 2003-02-05 2004-08-11 ARTEMIS Pharmaceuticals GmbH Automated gene-targeting using non-toxic detectable markers
EP1623035A4 (en) 2003-04-15 2006-08-30 Univ Georgia Res Found AVIAN TRANSGENESE USING A REGION OF THE CHICKEN OVALBUMIN GENE
EP2395017A3 (en) 2003-05-30 2012-12-19 Merus B.V. Design and use of paired variable regions of specific binding molecules
WO2005005644A1 (en) 2003-07-11 2005-01-20 Cytos Biotechnology Ag Gene expression system
EP1668137B8 (en) 2003-08-27 2012-03-21 ORF Liftaekni HF. Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins
ATE484591T1 (de) 2003-10-21 2010-10-15 Merck Serono Sa Minimale dna sequenz, die als chromatin-isolator wirkt, und deren verwendung für die protein- expression
IN2014DN02484A (enExample) * 2003-10-24 2015-05-15 Selexis Sa
SG176501A1 (en) 2006-08-23 2011-12-29 Selexis Sa Matrix attachment regions (mars) for increasing transcription and uses thereof
EP2097538A4 (en) * 2006-12-07 2011-11-30 Switchgear Genomics TRANSCRIPTION REAGULATION ELEMENTS OF BIOLOGICAL PATHS, TOOLS AND METHODS

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