KR100819228B1 - 신규의 니트릴히드라타제 - Google Patents

신규의 니트릴히드라타제 Download PDF

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KR100819228B1
KR100819228B1 KR1020077024119A KR20077024119A KR100819228B1 KR 100819228 B1 KR100819228 B1 KR 100819228B1 KR 1020077024119 A KR1020077024119 A KR 1020077024119A KR 20077024119 A KR20077024119 A KR 20077024119A KR 100819228 B1 KR100819228 B1 KR 100819228B1
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amino acid
seq
nitrile hydratase
subunit
sequence
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KR20070110146A (ko
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토시후미 야마키
신이치 반바
카오리 마토이시
키요시 이토
히데키 고바야시
에이시 타나카
토시히로 오이카와
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미쓰이 가가쿠 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01084—Nitrile hydratase (4.2.1.84)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020077024119A 2002-12-19 2003-12-15 신규의 니트릴히드라타제 Expired - Lifetime KR100819228B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JPJP-P-2002-00368360 2002-12-19
JP2002368360A JP2004194588A (ja) 2002-12-19 2002-12-19 新規なニトリルヒドラターゼ
JPJP-P-2003-00379280 2003-11-10
JP2003379280 2003-11-10

Related Parent Applications (1)

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KR1020057011335A Division KR100825147B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제

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KR20070110146A KR20070110146A (ko) 2007-11-15
KR100819228B1 true KR100819228B1 (ko) 2008-04-03

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Application Number Title Priority Date Filing Date
KR1020077024119A Expired - Lifetime KR100819228B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020057011335A Expired - Lifetime KR100825147B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020077015994A Expired - Lifetime KR100794001B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026866A Expired - Lifetime KR100785548B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026865A Expired - Lifetime KR100785549B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제

Family Applications After (4)

Application Number Title Priority Date Filing Date
KR1020057011335A Expired - Lifetime KR100825147B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020077015994A Expired - Lifetime KR100794001B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026866A Expired - Lifetime KR100785548B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제
KR1020067026865A Expired - Lifetime KR100785549B1 (ko) 2002-12-19 2003-12-15 신규의 니트릴히드라타제

Country Status (7)

Country Link
US (1) US7595184B2 (https=)
EP (1) EP1586637B1 (https=)
JP (1) JP2004194588A (https=)
KR (5) KR100819228B1 (https=)
CN (4) CN101892211A (https=)
AU (2) AU2003289338A1 (https=)
WO (1) WO2004056990A1 (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1767624B1 (en) * 2004-05-26 2013-11-06 Mitsubishi Rayon Co., Ltd. Improved nitrile hydratase
EP1842907A1 (en) 2006-04-07 2007-10-10 B.R.A.I.N. Ag A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
JP5069032B2 (ja) * 2007-04-04 2012-11-07 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
AU2009315170C1 (en) * 2008-11-14 2014-10-23 Mitsui Chemicals, Inc. Nitrile hydratase variant
JP5532618B2 (ja) * 2009-01-30 2014-06-25 三菱レイヨン株式会社 改良型ニトリルヒドラターゼ及びその製造方法
WO2010125829A1 (ja) 2009-04-30 2010-11-04 三井化学株式会社 ３-メルカプトプロピオン酸またはその塩を製造する方法
KR101734797B1 (ko) 2011-05-31 2017-05-24 미쯔비시 케미컬 주식회사 개량형 니트릴 히드라타제
US9193966B2 (en) 2011-06-07 2015-11-24 Mitsubishi Rayon Co., Ltd. Nitrile hydratase
CN102517271B (zh) * 2011-12-13 2013-04-03 清华大学 一种突变腈水合酶
CN112941060B (zh) 2014-06-06 2025-05-09 三菱化学株式会社 改良型腈水合酶
CN104725476B (zh) * 2015-03-31 2018-10-16 清华大学 一种钩状结构寡肽及其应用
AU2017388945B2 (en) * 2016-12-28 2021-06-03 Mitsui Chemicals, Inc. Mutant nitrile hydratase, nucleic acid coding said mutant nitrile hydratase, expression vector and transformant including said nucleic acid, production method for said mutant nitrile hydratase, and production method for amide compound
KR20190049109A (ko) 2017-11-01 2019-05-09 (주)대화정공 풀리 제조방법
KR102068615B1 (ko) 2019-04-08 2020-01-21 (주)대화정공 자동차 댐퍼풀리 제조방법
KR102146408B1 (ko) 2019-04-08 2020-08-21 (주)대화정공 단류선 개선을 위해 압연롤러를 이용한 자동차 댐퍼풀리 제조방법
CN111690675B (zh) * 2019-08-01 2022-07-19 安徽瑞邦生物科技有限公司 一种表达腈水合酶突变体的重组菌及其制备方法和应用
CN114790452B (zh) * 2020-11-20 2024-03-26 江南大学 一种对腈类化合物具有高稳定性的腈水合酶突变体
CN112522245A (zh) * 2020-12-09 2021-03-19 江南大学 一种腈水合酶氨基酸基序的改造及其应用
CN113151234B (zh) * 2021-04-13 2022-08-12 浙江工业大学 腈水合酶赖氨酸突变体hba-k2h2r、编码基因及应用
CN113444714B (zh) * 2021-07-29 2022-06-21 浙江大学杭州国际科创中心 一种β亚基突变的腈水合酶突变体及其应用
CN114317507B (zh) * 2021-11-30 2024-07-02 清华大学 腈水合酶突变体及其应用
CN119343452A (zh) * 2022-06-30 2025-01-21 三井化学株式会社 突变型腈水合酶、编码该突变型腈水合酶的核酸、含有该核酸的载体及转化体、该突变型腈水合酶的制造方法、及酰胺化合物的制造方法
CN115772514B (zh) * 2022-11-03 2025-03-04 无锡新晨宇生物工程有限公司 一种腈水合酶底物通道氨基酸基序的改造用于肉桂酰胺的制备
EP4600368A1 (en) * 2024-02-09 2025-08-13 Adisseo France S.A.S. A method for enzymatic production of an amide compound

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09275978A (ja) * 1996-02-14 1997-10-28 Mitsui Toatsu Chem Inc 新規なニトリルヒドラターゼ

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX169933B (es) 1987-09-18 1993-08-02 Hideaki Yamada Procedimiento para la produccion biologica de amidas
AU627648B2 (en) 1990-02-28 1992-08-27 Teruhiko Beppu Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
JPH0856684A (ja) 1994-08-16 1996-03-05 Mitsui Toatsu Chem Inc 微生物によるアミド化合物の製造法
CN1240833C (zh) 1996-02-14 2006-02-08 三井化学株式会社 新型腈水合酶
JP3408737B2 (ja) * 1998-03-16 2003-05-19 三井化学株式会社 ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09275978A (ja) * 1996-02-14 1997-10-28 Mitsui Toatsu Chem Inc 新規なニトリルヒドラターゼ

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NCBI sequence database
일본공개특허공보 특개평9-275978호

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Publication number Publication date
KR20070007393A (ko) 2007-01-15
EP1586637B1 (en) 2014-02-26
WO2004056990A1 (ja) 2004-07-08
AU2010202683B2 (en) 2012-01-19
KR100785549B1 (ko) 2007-12-12
AU2010202683A1 (en) 2010-07-15
KR20070110146A (ko) 2007-11-15
JP2004194588A (ja) 2004-07-15
KR100794001B1 (ko) 2008-01-10
CN103215242A (zh) 2013-07-24
EP1586637A4 (en) 2008-03-19
EP1586637A1 (en) 2005-10-19
KR100785548B1 (ko) 2007-12-12
KR20070087054A (ko) 2007-08-27
US7595184B2 (en) 2009-09-29
KR100825147B1 (ko) 2008-04-24
KR20050089972A (ko) 2005-09-09
AU2003289338A1 (en) 2004-07-14
CN103103175A (zh) 2013-05-15
KR20070011620A (ko) 2007-01-24
US20070009985A1 (en) 2007-01-11
CN1729288A (zh) 2006-02-01
CN101892211A (zh) 2010-11-24

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