KR100795760B1 - 영장류 배아 줄기 세포의 무혈청 배양 - Google Patents
영장류 배아 줄기 세포의 무혈청 배양 Download PDFInfo
- Publication number
- KR100795760B1 KR100795760B1 KR1020027011681A KR20027011681A KR100795760B1 KR 100795760 B1 KR100795760 B1 KR 100795760B1 KR 1020027011681 A KR1020027011681 A KR 1020027011681A KR 20027011681 A KR20027011681 A KR 20027011681A KR 100795760 B1 KR100795760 B1 KR 100795760B1
- Authority
- KR
- South Korea
- Prior art keywords
- stem cells
- growth factor
- embryonic stem
- serum
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Gynecology & Obstetrics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/522,030 | 2000-03-09 | ||
| US09/522,030 US7005252B1 (en) | 2000-03-09 | 2000-03-09 | Serum free cultivation of primate embryonic stem cells |
| PCT/US2001/006912 WO2001066697A2 (en) | 2000-03-09 | 2001-03-02 | Serum free cultivation of primate embryonic stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20030032926A KR20030032926A (ko) | 2003-04-26 |
| KR100795760B1 true KR100795760B1 (ko) | 2008-01-21 |
Family
ID=24079156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020027011681A Expired - Lifetime KR100795760B1 (ko) | 2000-03-09 | 2001-03-02 | 영장류 배아 줄기 세포의 무혈청 배양 |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US7005252B1 (enExample) |
| EP (1) | EP1261691B1 (enExample) |
| JP (3) | JP5717311B2 (enExample) |
| KR (1) | KR100795760B1 (enExample) |
| CN (1) | CN100372928C (enExample) |
| AU (2) | AU2001241973B2 (enExample) |
| BR (1) | BR0108507A (enExample) |
| CA (1) | CA2402299C (enExample) |
| IL (2) | IL151270A0 (enExample) |
| IS (1) | IS6515A (enExample) |
| MX (1) | MXPA02008698A (enExample) |
| NO (1) | NO335780B1 (enExample) |
| NZ (1) | NZ520701A (enExample) |
| WO (1) | WO2001066697A2 (enExample) |
Families Citing this family (152)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7413904B2 (en) * | 1998-10-23 | 2008-08-19 | Geron Corporation | Human embryonic stem cells having genetic modifications |
| US7410798B2 (en) * | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US8252280B1 (en) | 1999-08-05 | 2012-08-28 | Regents Of The University Of Minnesota | MAPC generation of muscle |
| US10638734B2 (en) | 2004-01-05 | 2020-05-05 | Abt Holding Company | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof |
| US7015037B1 (en) | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
| US7455983B2 (en) * | 2000-01-11 | 2008-11-25 | Geron Corporation | Medium for growing human embryonic stem cells |
| US20100173410A1 (en) * | 2000-03-09 | 2010-07-08 | Wicell Research Institute, Inc. | Cultivation of Primate Embryonic Stem Cells |
| US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| IL157332A0 (en) | 2001-02-14 | 2004-02-19 | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof | |
| AU2002354893A1 (en) * | 2001-07-20 | 2003-03-03 | Technion Research And Development Foundation Ltd. | Methods of generating human cardiac cells and tissues and uses thereof |
| WO2003046129A2 (en) * | 2001-11-09 | 2003-06-05 | Trustees Of The University Of Pennsylvania | Compositions for the derivation of germ cells from stem cells and methods of use thereof |
| GB0202149D0 (en) | 2002-01-30 | 2002-03-20 | Univ Edinburgh | Pluripotency determining factors and uses thereof |
| US20040111285A1 (en) * | 2002-04-09 | 2004-06-10 | Mark Germain | Method for human pluripotent stem cells |
| AU2003260724A1 (en) * | 2002-08-22 | 2004-03-11 | Celltran Limited | Cell culture surface |
| CN1717478A (zh) * | 2002-10-25 | 2006-01-04 | 湖南惠霖生命科技有限公司 | 用于人类胚胎干细胞体外培养的饲养细胞层以及培养胚胎干细胞的方法 |
| GB0304918D0 (en) * | 2003-03-05 | 2003-04-09 | Celltran Ltd | Cell culture |
| US7820439B2 (en) | 2003-09-03 | 2010-10-26 | Reliance Life Sciences Pvt Ltd. | In vitro generation of GABAergic neurons from pluripotent stem cells |
| CA2547827C (en) | 2003-12-02 | 2015-02-03 | Catholic Healthcare West | Compositions and methods for propagation of neural progenitor cells |
| US20070269412A1 (en) | 2003-12-02 | 2007-11-22 | Celavie Biosciences, Llc | Pluripotent cells |
| US7985585B2 (en) | 2004-07-09 | 2011-07-26 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
| CN103898047B (zh) * | 2003-12-23 | 2020-03-03 | 维亚希特公司 | 定形内胚层 |
| US7541185B2 (en) * | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
| WO2007059007A2 (en) | 2005-11-14 | 2007-05-24 | Cythera, Inc. | Markers of definitive endoderm |
| US7625753B2 (en) * | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| US8647873B2 (en) | 2004-04-27 | 2014-02-11 | Viacyte, Inc. | PDX1 expressing endoderm |
| US20050266554A1 (en) * | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| WO2005065354A2 (en) * | 2003-12-31 | 2005-07-21 | The Burnham Institute | Defined media for pluripotent stem cell culture |
| US20080085554A1 (en) * | 2004-02-13 | 2008-04-10 | Norio Nakatsuji | Culture Medium for Culturing Feeder Cells for Embryonic Stem Cells Culture and the Prepared Feeder Cells |
| JP4688793B2 (ja) * | 2004-03-23 | 2011-05-25 | 敏宏 赤池 | 多能性幹細胞の増殖方法 |
| EP2377922B1 (en) | 2004-04-27 | 2020-04-08 | Viacyte, Inc. | PDX1 expressing endoderm |
| EP1749091B1 (en) | 2004-05-21 | 2013-07-31 | Wicell Research Institute, Inc. | Feeder independent extended culture of embryonic stem cells |
| EP3505622A1 (en) | 2004-07-09 | 2019-07-03 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
| WO2006020919A2 (en) * | 2004-08-13 | 2006-02-23 | University Of Georgia Research Foundation, Inc. | Compositions and methods for self-renewal and differentiation in human embryonic stem cells |
| KR101264940B1 (ko) | 2004-09-08 | 2013-05-15 | 위스콘신 얼럼나이 리서어치 화운데이션 | 배아 줄기 세포용 배지 및 이의 배양물 |
| ES2800973T3 (es) * | 2004-09-28 | 2021-01-07 | Wisconsin Alumni Res Found | Cultivo de células madre embrionarias de primates |
| US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
| US20060263879A1 (en) * | 2004-12-30 | 2006-11-23 | Stemlifeline, Inc. | Methods and systems relating to embryonic stem cell lines |
| WO2006073911A1 (en) * | 2004-12-30 | 2006-07-13 | Stemlifeline, Inc. | Methods and compositions relating to embryonic stem cell lines |
| CN1298843C (zh) * | 2005-02-07 | 2007-02-07 | 十堰市太和医院 | 不依赖饲养细胞的人胚干细胞培养基 |
| AU2006202209B2 (en) * | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| ES2400916T3 (es) * | 2005-06-08 | 2013-04-15 | Janssen Biotech, Inc. | Una terapia celular para degeneración ocular |
| EP1910516B1 (en) | 2005-06-22 | 2019-06-19 | Asterias Biotherapeutics, Inc. | Suspension culture of human embryonic stem cells |
| US9101590B2 (en) * | 2005-07-29 | 2015-08-11 | Yale University | Defined culture conditions of human embryonic stem cells |
| KR100670616B1 (ko) * | 2005-08-25 | 2007-01-17 | 주식회사 메디아나전자 | 상피세포 또는 섬유아세포를 이용한 세포 배양용 인공 배아 및 세포 배양 장치 |
| US20080199849A1 (en) * | 2005-09-02 | 2008-08-21 | Agency For Science, Technology And Research | Method of Deriving Progenitor Cell Line |
| WO2007044572A2 (en) | 2005-10-06 | 2007-04-19 | University Of Massachusetts | Cell division marker |
| US20100129906A1 (en) * | 2005-10-07 | 2010-05-27 | Cellartis Ab | Method for Obtaining Xeno-Free Hbs Cell line |
| JP5404047B2 (ja) | 2005-10-27 | 2014-01-29 | ヴィアサイト，インコーポレイテッド | Ｐｄｘ１発現背側及び腹側前腸内胚葉 |
| US7413900B2 (en) * | 2005-10-31 | 2008-08-19 | President And Fellows Of Harvard College | Immortalized fibroblasts |
| US11254916B2 (en) | 2006-03-02 | 2022-02-22 | Viacyte, Inc. | Methods of making and using PDX1-positive pancreatic endoderm cells |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| EP1999253B1 (en) | 2006-03-02 | 2019-05-22 | Viacyte, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
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| BR0108507A (pt) | 2002-12-17 |
| CA2402299C (en) | 2012-12-18 |
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