BR0108507A - Cultivo livre de soro de células-tronco embriÈnicas de primatas - Google Patents

Cultivo livre de soro de células-tronco embriÈnicas de primatas

Info

Publication number
BR0108507A
BR0108507A BR0108507-7A BR0108507A BR0108507A BR 0108507 A BR0108507 A BR 0108507A BR 0108507 A BR0108507 A BR 0108507A BR 0108507 A BR0108507 A BR 0108507A
Authority
BR
Brazil
Prior art keywords
serum
embryonic stem
stem cells
primate embryonic
free culture
Prior art date
Application number
BR0108507-7A
Other languages
English (en)
Inventor
James A Thomson
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24079156&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR0108507(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of BR0108507A publication Critical patent/BR0108507A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"CULTIVO LIVRE DE SORO DE CéLULAS-TRONCO EMBRIÈNICAS DE PRIMATAS". São aqui revelados métodos de cultivo de células-tronco embriónicas primatas. Essas células são cultivadas sobre uma base prolongada e estável em presença de fator de crescimento de fibroblastos fornecido exogenamente e em ausência de soro animal. Preferivelmente existe também uma camada alimentadora de fibroblastos. é também revelado um meio de cultura contendo a camada alimentadora de fibroblastos e o fator de crescimento de fibroblastos.
BR0108507-7A 2000-03-09 2001-03-02 Cultivo livre de soro de células-tronco embriÈnicas de primatas BR0108507A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/522,030 US7005252B1 (en) 2000-03-09 2000-03-09 Serum free cultivation of primate embryonic stem cells
PCT/US2001/006912 WO2001066697A2 (en) 2000-03-09 2001-03-02 Serum free cultivation of primate embryonic stem cells

Publications (1)

Publication Number Publication Date
BR0108507A true BR0108507A (pt) 2002-12-17

Family

ID=24079156

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0108507-7A BR0108507A (pt) 2000-03-09 2001-03-02 Cultivo livre de soro de células-tronco embriÈnicas de primatas

Country Status (15)

Country Link
US (4) US7005252B1 (pt)
EP (1) EP1261691B1 (pt)
JP (3) JP5717311B2 (pt)
KR (1) KR100795760B1 (pt)
CN (1) CN100372928C (pt)
AU (2) AU4197301A (pt)
BR (1) BR0108507A (pt)
CA (1) CA2402299C (pt)
HK (1) HK1053616A1 (pt)
IL (2) IL151270A0 (pt)
IS (1) IS6515A (pt)
MX (1) MXPA02008698A (pt)
NO (1) NO335780B1 (pt)
NZ (1) NZ520701A (pt)
WO (1) WO2001066697A2 (pt)

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