CA2402299C - Serum free cultivation of primate embryonic stem cells - Google Patents
Serum free cultivation of primate embryonic stem cells Download PDFInfo
- Publication number
- CA2402299C CA2402299C CA2402299A CA2402299A CA2402299C CA 2402299 C CA2402299 C CA 2402299C CA 2402299 A CA2402299 A CA 2402299A CA 2402299 A CA2402299 A CA 2402299A CA 2402299 C CA2402299 C CA 2402299C
- Authority
- CA
- Canada
- Prior art keywords
- culture
- stem cells
- embryonic stem
- serum
- growth factor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Gynecology & Obstetrics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/522,030 | 2000-03-09 | ||
| US09/522,030 US7005252B1 (en) | 2000-03-09 | 2000-03-09 | Serum free cultivation of primate embryonic stem cells |
| PCT/US2001/006912 WO2001066697A2 (en) | 2000-03-09 | 2001-03-02 | Serum free cultivation of primate embryonic stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2402299A1 CA2402299A1 (en) | 2001-09-13 |
| CA2402299C true CA2402299C (en) | 2012-12-18 |
Family
ID=24079156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2402299A Expired - Lifetime CA2402299C (en) | 2000-03-09 | 2001-03-02 | Serum free cultivation of primate embryonic stem cells |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US7005252B1 (enExample) |
| EP (1) | EP1261691B1 (enExample) |
| JP (3) | JP5717311B2 (enExample) |
| KR (1) | KR100795760B1 (enExample) |
| CN (1) | CN100372928C (enExample) |
| AU (2) | AU2001241973B2 (enExample) |
| BR (1) | BR0108507A (enExample) |
| CA (1) | CA2402299C (enExample) |
| IL (2) | IL151270A0 (enExample) |
| IS (1) | IS6515A (enExample) |
| MX (1) | MXPA02008698A (enExample) |
| NO (1) | NO335780B1 (enExample) |
| NZ (1) | NZ520701A (enExample) |
| WO (1) | WO2001066697A2 (enExample) |
Families Citing this family (152)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7413904B2 (en) * | 1998-10-23 | 2008-08-19 | Geron Corporation | Human embryonic stem cells having genetic modifications |
| US7410798B2 (en) * | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US8252280B1 (en) | 1999-08-05 | 2012-08-28 | Regents Of The University Of Minnesota | MAPC generation of muscle |
| US10638734B2 (en) | 2004-01-05 | 2020-05-05 | Abt Holding Company | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof |
| US7015037B1 (en) | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
| US7455983B2 (en) * | 2000-01-11 | 2008-11-25 | Geron Corporation | Medium for growing human embryonic stem cells |
| US20100173410A1 (en) * | 2000-03-09 | 2010-07-08 | Wicell Research Institute, Inc. | Cultivation of Primate Embryonic Stem Cells |
| US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| CA2438501C (en) | 2001-02-14 | 2014-09-16 | Leo T. Furcht | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof |
| IL159895A0 (en) * | 2001-07-20 | 2004-06-20 | Technion Res & Dev Foundation | Methods of generating human cardiac cells and tissues and uses thereof |
| US7704736B2 (en) * | 2001-11-09 | 2010-04-27 | Trustees Of The University Of Pennsylvania | Compositions for the derivation of germ cells from stem cells and methods of use thereof |
| GB0202149D0 (en) | 2002-01-30 | 2002-03-20 | Univ Edinburgh | Pluripotency determining factors and uses thereof |
| US20040111285A1 (en) * | 2002-04-09 | 2004-06-10 | Mark Germain | Method for human pluripotent stem cells |
| US20050244961A1 (en) * | 2002-08-22 | 2005-11-03 | Robert Short | Cell culture surface |
| WO2004038012A1 (en) * | 2002-10-25 | 2004-05-06 | Hunan Hui-Lin Life Technology Co. Ltd | The feeder cell layer for in vitro culturing human embryonic stem cells and the method for culturing embryonic stem cells |
| GB0304918D0 (en) * | 2003-03-05 | 2003-04-09 | Celltran Ltd | Cell culture |
| US7820439B2 (en) | 2003-09-03 | 2010-10-26 | Reliance Life Sciences Pvt Ltd. | In vitro generation of GABAergic neurons from pluripotent stem cells |
| US20070269412A1 (en) * | 2003-12-02 | 2007-11-22 | Celavie Biosciences, Llc | Pluripotent cells |
| JP4676442B2 (ja) | 2003-12-02 | 2011-04-27 | セラヴィー バイオサイエンシズ エルエルシー | 神経前駆細胞を増殖させるための組成物および方法 |
| US7541185B2 (en) * | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
| US8647873B2 (en) | 2004-04-27 | 2014-02-11 | Viacyte, Inc. | PDX1 expressing endoderm |
| US7625753B2 (en) * | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| US7985585B2 (en) * | 2004-07-09 | 2011-07-26 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
| US20050266554A1 (en) * | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| KR101221302B1 (ko) | 2003-12-23 | 2013-01-11 | 비아싸이트, 인크. | 완전 내배엽 |
| WO2007059007A2 (en) | 2005-11-14 | 2007-05-24 | Cythera, Inc. | Markers of definitive endoderm |
| WO2005065354A2 (en) * | 2003-12-31 | 2005-07-21 | The Burnham Institute | Defined media for pluripotent stem cell culture |
| EP1715033A4 (en) * | 2004-02-13 | 2008-06-11 | Asahi Techno Glass Corp | MEDIUM FOR PREPARATION OF FEEDER CELLS FOR EMBRYONIC STEM CELLS AND FEEDER CELLS |
| JP4688793B2 (ja) * | 2004-03-23 | 2011-05-25 | 敏宏 赤池 | 多能性幹細胞の増殖方法 |
| EP2377922B1 (en) | 2004-04-27 | 2020-04-08 | Viacyte, Inc. | PDX1 expressing endoderm |
| JP5128946B2 (ja) * | 2004-05-21 | 2013-01-23 | ウィセル リサーチ インスティテュート インコーポレイテッド | 胚性幹細胞のフィーダー非依存性長期培養 |
| ES2754038T3 (es) | 2004-07-09 | 2020-04-15 | Viacyte Inc | Células mesendodérmicas y células de línea pre-primitiva |
| EP1791952A4 (en) * | 2004-08-13 | 2008-06-11 | Univ Georgia Res Found | COMPOSITIONS AND METHODS OF SELF-RENEWAL AND DIFFERENTIATION IN HUMAN EMBRYONAL STEM CELLS |
| ES2383813T3 (es) | 2004-09-08 | 2012-06-26 | Wisconsin Alumni Research Foundation | Método de cultivo y cultivo de células madre embrionarias |
| ES2800973T3 (es) * | 2004-09-28 | 2021-01-07 | Wisconsin Alumni Res Found | Cultivo de células madre embrionarias de primates |
| US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
| EP1844139A1 (en) * | 2004-12-30 | 2007-10-17 | Stemlifeline, Inc. | Methods and systems relating to embryonic stem cell lines |
| EP1841857A1 (en) * | 2004-12-30 | 2007-10-10 | Stemlifeline, Inc. | Methods and compositions relating to embryonic stem cell lines |
| CN1298843C (zh) * | 2005-02-07 | 2007-02-07 | 十堰市太和医院 | 不依赖饲养细胞的人胚干细胞培养基 |
| AU2006202209B2 (en) * | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| CA2613889A1 (en) * | 2005-06-08 | 2006-12-14 | Centocor, Inc. | A cellular therapy for ocular degeneration |
| AU2006262369B2 (en) | 2005-06-22 | 2012-07-05 | Asterias Biotherapeutics, Inc. | Suspension culture of human embryonic stem cells |
| US9101590B2 (en) * | 2005-07-29 | 2015-08-11 | Yale University | Defined culture conditions of human embryonic stem cells |
| KR100670616B1 (ko) * | 2005-08-25 | 2007-01-17 | 주식회사 메디아나전자 | 상피세포 또는 섬유아세포를 이용한 세포 배양용 인공 배아 및 세포 배양 장치 |
| BRPI0617084A2 (pt) * | 2005-09-02 | 2011-07-12 | Agency Science Tech & Res | método, linhagem de célula progenitora, célula diferenciada e método para gerar uma célula diferenciada de uma célula-tronco (es) embrionária |
| US7682799B2 (en) * | 2005-10-06 | 2010-03-23 | University Of Massachusetts | Cell division marker |
| WO2007042225A2 (en) * | 2005-10-07 | 2007-04-19 | Cellartis Ab | A method for obtaining a xeno-free hbs cell line |
| EP3584311A1 (en) | 2005-10-27 | 2019-12-25 | Viacyte, Inc. | Pdx-1 expressing dorsal and ventral foregut endoderm |
| US7413900B2 (en) * | 2005-10-31 | 2008-08-19 | President And Fellows Of Harvard College | Immortalized fibroblasts |
| CA2644468C (en) | 2006-03-02 | 2022-02-01 | Cythera, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| US11254916B2 (en) | 2006-03-02 | 2022-02-22 | Viacyte, Inc. | Methods of making and using PDX1-positive pancreatic endoderm cells |
| US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| US7989204B2 (en) * | 2006-04-28 | 2011-08-02 | Viacyte, Inc. | Hepatocyte lineage cells |
| EP2021462B1 (en) | 2006-04-28 | 2019-01-09 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
| CN100465268C (zh) * | 2006-05-17 | 2009-03-04 | 北京大学 | 人胚胎干细胞的培养方法及其专用培养基 |
| US7964402B2 (en) * | 2006-05-25 | 2011-06-21 | Sanford-Burnham Medical Research Institute | Methods for culture and production of single cell populations of human embryonic stem cells |
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