CN100372928C - 灵长类动物胚胎干细胞的无血清培养物 - Google Patents

灵长类动物胚胎干细胞的无血清培养物 Download PDF

Info

Publication number
CN100372928C
CN100372928C CNB018062350A CN01806235A CN100372928C CN 100372928 C CN100372928 C CN 100372928C CN B018062350 A CNB018062350 A CN B018062350A CN 01806235 A CN01806235 A CN 01806235A CN 100372928 C CN100372928 C CN 100372928C
Authority
CN
China
Prior art keywords
embryonic stem
serum
stem cells
growth factor
fibroblast growth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CNB018062350A
Other languages
English (en)
Chinese (zh)
Other versions
CN1416345A (zh
Inventor
J·A·汤姆森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24079156&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN100372928(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of CN1416345A publication Critical patent/CN1416345A/zh
Application granted granted Critical
Publication of CN100372928C publication Critical patent/CN100372928C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CNB018062350A 2000-03-09 2001-03-02 灵长类动物胚胎干细胞的无血清培养物 Expired - Lifetime CN100372928C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/522,030 2000-03-09
US09/522,030 US7005252B1 (en) 2000-03-09 2000-03-09 Serum free cultivation of primate embryonic stem cells

Publications (2)

Publication Number Publication Date
CN1416345A CN1416345A (zh) 2003-05-07
CN100372928C true CN100372928C (zh) 2008-03-05

Family

ID=24079156

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB018062350A Expired - Lifetime CN100372928C (zh) 2000-03-09 2001-03-02 灵长类动物胚胎干细胞的无血清培养物

Country Status (14)

Country Link
US (4) US7005252B1 (enExample)
EP (1) EP1261691B1 (enExample)
JP (3) JP5717311B2 (enExample)
KR (1) KR100795760B1 (enExample)
CN (1) CN100372928C (enExample)
AU (2) AU2001241973B2 (enExample)
BR (1) BR0108507A (enExample)
CA (1) CA2402299C (enExample)
IL (2) IL151270A0 (enExample)
IS (1) IS6515A (enExample)
MX (1) MXPA02008698A (enExample)
NO (1) NO335780B1 (enExample)
NZ (1) NZ520701A (enExample)
WO (1) WO2001066697A2 (enExample)

Families Citing this family (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7413904B2 (en) * 1998-10-23 2008-08-19 Geron Corporation Human embryonic stem cells having genetic modifications
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US8252280B1 (en) 1999-08-05 2012-08-28 Regents Of The University Of Minnesota MAPC generation of muscle
US10638734B2 (en) 2004-01-05 2020-05-05 Abt Holding Company Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
US7015037B1 (en) 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US20100173410A1 (en) * 2000-03-09 2010-07-08 Wicell Research Institute, Inc. Cultivation of Primate Embryonic Stem Cells
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
CA2438501C (en) 2001-02-14 2014-09-16 Leo T. Furcht Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
IL159895A0 (en) * 2001-07-20 2004-06-20 Technion Res & Dev Foundation Methods of generating human cardiac cells and tissues and uses thereof
US7704736B2 (en) * 2001-11-09 2010-04-27 Trustees Of The University Of Pennsylvania Compositions for the derivation of germ cells from stem cells and methods of use thereof
GB0202149D0 (en) 2002-01-30 2002-03-20 Univ Edinburgh Pluripotency determining factors and uses thereof
US20040111285A1 (en) * 2002-04-09 2004-06-10 Mark Germain Method for human pluripotent stem cells
US20050244961A1 (en) * 2002-08-22 2005-11-03 Robert Short Cell culture surface
WO2004038012A1 (en) * 2002-10-25 2004-05-06 Hunan Hui-Lin Life Technology Co. Ltd The feeder cell layer for in vitro culturing human embryonic stem cells and the method for culturing embryonic stem cells
GB0304918D0 (en) * 2003-03-05 2003-04-09 Celltran Ltd Cell culture
US7820439B2 (en) 2003-09-03 2010-10-26 Reliance Life Sciences Pvt Ltd. In vitro generation of GABAergic neurons from pluripotent stem cells
US20070269412A1 (en) * 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
JP4676442B2 (ja) 2003-12-02 2011-04-27 セラヴィー バイオサイエンシズ エルエルシー 神経前駆細胞を増殖させるための組成物および方法
US7541185B2 (en) * 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7625753B2 (en) * 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US7985585B2 (en) * 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
US20050266554A1 (en) * 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
KR101221302B1 (ko) 2003-12-23 2013-01-11 비아싸이트, 인크. 완전 내배엽
WO2007059007A2 (en) 2005-11-14 2007-05-24 Cythera, Inc. Markers of definitive endoderm
WO2005065354A2 (en) * 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
EP1715033A4 (en) * 2004-02-13 2008-06-11 Asahi Techno Glass Corp MEDIUM FOR PREPARATION OF FEEDER CELLS FOR EMBRYONIC STEM CELLS AND FEEDER CELLS
JP4688793B2 (ja) * 2004-03-23 2011-05-25 敏宏 赤池 多能性幹細胞の増殖方法
EP2377922B1 (en) 2004-04-27 2020-04-08 Viacyte, Inc. PDX1 expressing endoderm
JP5128946B2 (ja) * 2004-05-21 2013-01-23 ウィセル リサーチ インスティテュート インコーポレイテッド 胚性幹細胞のフィーダー非依存性長期培養
ES2754038T3 (es) 2004-07-09 2020-04-15 Viacyte Inc Células mesendodérmicas y células de línea pre-primitiva
EP1791952A4 (en) * 2004-08-13 2008-06-11 Univ Georgia Res Found COMPOSITIONS AND METHODS OF SELF-RENEWAL AND DIFFERENTIATION IN HUMAN EMBRYONAL STEM CELLS
ES2383813T3 (es) 2004-09-08 2012-06-26 Wisconsin Alumni Research Foundation Método de cultivo y cultivo de células madre embrionarias
ES2800973T3 (es) * 2004-09-28 2021-01-07 Wisconsin Alumni Res Found Cultivo de células madre embrionarias de primates
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
EP1844139A1 (en) * 2004-12-30 2007-10-17 Stemlifeline, Inc. Methods and systems relating to embryonic stem cell lines
EP1841857A1 (en) * 2004-12-30 2007-10-10 Stemlifeline, Inc. Methods and compositions relating to embryonic stem cell lines
CN1298843C (zh) * 2005-02-07 2007-02-07 十堰市太和医院 不依赖饲养细胞的人胚干细胞培养基
AU2006202209B2 (en) * 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
CA2613889A1 (en) * 2005-06-08 2006-12-14 Centocor, Inc. A cellular therapy for ocular degeneration
AU2006262369B2 (en) 2005-06-22 2012-07-05 Asterias Biotherapeutics, Inc. Suspension culture of human embryonic stem cells
US9101590B2 (en) * 2005-07-29 2015-08-11 Yale University Defined culture conditions of human embryonic stem cells
KR100670616B1 (ko) * 2005-08-25 2007-01-17 주식회사 메디아나전자 상피세포 또는 섬유아세포를 이용한 세포 배양용 인공 배아 및 세포 배양 장치
BRPI0617084A2 (pt) * 2005-09-02 2011-07-12 Agency Science Tech & Res método, linhagem de célula progenitora, célula diferenciada e método para gerar uma célula diferenciada de uma célula-tronco (es) embrionária
US7682799B2 (en) * 2005-10-06 2010-03-23 University Of Massachusetts Cell division marker
WO2007042225A2 (en) * 2005-10-07 2007-04-19 Cellartis Ab A method for obtaining a xeno-free hbs cell line
EP3584311A1 (en) 2005-10-27 2019-12-25 Viacyte, Inc. Pdx-1 expressing dorsal and ventral foregut endoderm
US7413900B2 (en) * 2005-10-31 2008-08-19 President And Fellows Of Harvard College Immortalized fibroblasts
CA2644468C (en) 2006-03-02 2022-02-01 Cythera, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US7989204B2 (en) * 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
EP2021462B1 (en) 2006-04-28 2019-01-09 Lifescan, Inc. Differentiation of human embryonic stem cells
CN100465268C (zh) * 2006-05-17 2009-03-04 北京大学 人胚胎干细胞的培养方法及其专用培养基
US7964402B2 (en) * 2006-05-25 2011-06-21 Sanford-Burnham Medical Research Institute Methods for culture and production of single cell populations of human embryonic stem cells
AU2007261530B2 (en) * 2006-06-20 2014-02-06 Genzyme Corporation Serum-free media and their uses for chondrocyte expansion
WO2008018684A1 (en) * 2006-08-11 2008-02-14 Modern Cell & Tissue Technologies Inc. Culture medium for co-culturing of human stem cells and their feeder cells
US20100323442A1 (en) * 2006-10-17 2010-12-23 Emmanuel Edward Baetge Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells
AU2007321928A1 (en) 2006-11-24 2008-05-29 Regents Of The University Of Minnesota Endodermal progenitor cells
US10829733B2 (en) * 2007-01-04 2020-11-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
US7883698B2 (en) * 2007-01-17 2011-02-08 Maria Michejda Isolation and preservation of fetal hematopoietic and mesencymal system cells from non-controversial materials and/or tissues resulting from miscarriages and methods of therapeutic use
AU2008211103B2 (en) * 2007-01-30 2014-05-08 University Of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (MMC)
US7951593B2 (en) * 2007-03-20 2011-05-31 Universite Rene Descartes-Paris V Culture medium for gingival fibroblasts
US9080145B2 (en) * 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
WO2009006399A1 (en) 2007-07-01 2009-01-08 Lifescan Single pluripotent stem cell culture
BRPI0814425A2 (pt) 2007-07-18 2014-10-21 Lifescan Inc Diferenciação de células-tronco embrionárias humanas
JP6087043B2 (ja) 2007-07-31 2017-03-01 ライフスキャン・インコーポレイテッドＬｉｆｅｓｃａｎ，Ｉｎｃ． ヒトフィーダー細胞を用いた多能性幹細胞の分化
CA3114827C (en) 2007-07-31 2023-09-05 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
CA2954431C (en) * 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
US20100087002A1 (en) * 2008-02-21 2010-04-08 Benjamin Fryer Methods, Surface Modified Plates and Compositions for Cell Attachment, Cultivation and Detachment
RU2551772C2 (ru) 2008-02-21 2015-05-27 Сентокор Орто Байотек Инк. Способы, поверхностно-модифицированные носители и композиции для иммобилизации, культивирования и открепления клеток
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
EP2283117B1 (en) 2008-04-21 2013-10-23 Viacyte, Inc. Methods for purifying pancreatic endoderm cells derived from human embryonic stem cells
US8623648B2 (en) * 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US7939322B2 (en) * 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
PL2274430T3 (pl) 2008-04-30 2017-09-29 Sanbio Inc Komórki regenerujące nerwy ze zmianami w metylacji DNA
WO2009154606A1 (en) 2008-06-03 2009-12-23 Cythera, Inc. Growth factors for production of definitive endoderm
US20090298178A1 (en) * 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
AU2009267137A1 (en) 2008-06-30 2010-01-07 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
MX2011000123A (es) * 2008-06-30 2011-02-25 Centocor Ortho Biotech Inc Diferenciacion de las celulas madre pluripotentes.
US20110305672A1 (en) 2008-07-25 2011-12-15 University Of Georgia Research Foundation, Inc. COMPOSITIONS FOR MESODERM DERIVED ISL1+ MULTIPOTENT CELLS (IMPs), EPICARDIAL PROGENITOR CELLS (EPCs) AND MULTIPOTENT CD56C CELLS (C56Cs) AND METHODS OF PRODUCING AND USING SAME
WO2010011352A2 (en) 2008-07-25 2010-01-28 The University Of Georgia Research Foundation, Inc. Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
US20100028307A1 (en) * 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
MX2011004565A (es) 2008-10-31 2011-07-28 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
MX2011004563A (es) 2008-10-31 2011-06-01 Centocor Ortho Biotech Inc Diferenciacion de celulas madre embrionarias humanas al linaje endocrino pancreatico.
DK2356213T3 (da) 2008-11-04 2019-09-09 Viacyte Inc Stamcelleaggregatsuspensionssammensætninger og fremgangsmåder til differentiering deraf
EP3363444B1 (en) 2008-11-14 2022-09-14 ViaCyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
EP3260534A1 (en) 2008-11-20 2017-12-27 Janssen Biotech, Inc. Pluripotent stem cell culture on micro-carriers
BRPI0921996A2 (pt) * 2008-11-20 2015-08-18 Centocor Ortho Biotech Inc Métodos e composições para cultura e ligação de células em substratos planos.
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
US8642334B2 (en) 2009-02-17 2014-02-04 Memorial Sloan Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
US9109245B2 (en) 2009-04-22 2015-08-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
WO2010124142A2 (en) 2009-04-22 2010-10-28 Cythera, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
EP2456862A4 (en) 2009-07-20 2013-02-27 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
KR101785626B1 (ko) 2009-07-20 2017-10-16 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
KR101786735B1 (ko) 2009-07-20 2017-10-18 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
NZ598457A (en) * 2009-08-03 2014-06-27 Recombinetics Inc Methods and compositions for targeted gene modification
MX350966B (es) * 2009-10-29 2017-09-26 Janssen Biotech Inc Celulas madre pluripotentes.
WO2011063005A2 (en) 2009-11-17 2011-05-26 Advanced Cell Technology, Inc. Methods of producing human rpe cells and pharmaceutical preparations of human rpe cells
BR112012017761A2 (pt) 2009-12-23 2015-09-15 Centocor Ortho Biotech Inc diferenciação das células-tronco embrionárias humanas
PH12012501254A1 (en) * 2009-12-23 2012-11-05 Janssen Biotech Inc Differentiation of human embryonic stem cells
US9969981B2 (en) * 2010-03-01 2018-05-15 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
RU2663339C1 (ru) 2010-05-12 2018-08-03 Янссен Байотек, Инк. Дифференцирование эмбриональных стволовых клеток человека
EP4245370A3 (en) 2010-07-12 2023-11-29 University of Southern California Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
KR20190027969A (ko) 2010-08-12 2019-03-15 얀센 바이오테크 인코포레이티드 췌장 내분비 전구 세포를 이용한 당뇨병의 치료
CA2809300A1 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP6133776B2 (ja) 2010-08-31 2017-05-24 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞の分化
KR101836850B1 (ko) 2010-08-31 2018-03-09 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
US9732128B2 (en) 2010-10-22 2017-08-15 Biotime, Inc. Methods of modifying transcriptional regulatory networks in stem cells
US8415156B2 (en) * 2010-12-17 2013-04-09 Biolamina Ab Recombinant laminin-521
CA2829006A1 (en) 2011-03-04 2012-09-13 The Regents Of The University Of California Locally released growth factors to mediate motor recovery after stroke
US8785190B2 (en) 2011-04-06 2014-07-22 Sanbio, Inc. Methods and compositions for modulating peripheral immune function
US8877489B2 (en) 2011-12-05 2014-11-04 California Institute Of Technology Ultrathin parylene-C semipermeable membranes for biomedical applications
US10478206B2 (en) 2011-04-29 2019-11-19 University Of Southern California Instruments and methods for the implantation of cell-seeded substrates
WO2012170853A1 (en) 2011-06-10 2012-12-13 Wisconsin Alumni Research Foundation ("Warf") Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
CN110684734A (zh) 2011-11-04 2020-01-14 纪念斯隆-凯特琳癌症中心 用于植入的中脑多巴胺（da）神经元
US9248013B2 (en) 2011-12-05 2016-02-02 California Institute Of Technology 3-Dimensional parylene scaffold cage
AU2012355698B2 (en) 2011-12-22 2018-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
RU2018128383A (ru) 2012-03-07 2019-03-14 Янссен Байотек, Инк. Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток
KR20160027218A (ko) 2012-05-23 2016-03-09 에프. 호프만-라 로슈 아게 내배엽 및 간세포를 수득하고 사용하는 조성물 및 방법
RU2018108850A (ru) 2012-06-08 2019-02-26 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки
CN102732477B (zh) * 2012-06-15 2013-06-19 江苏瑞思坦生物科技有限公司 人脂肪干细胞无血清基础培养基
JP6297559B2 (ja) 2012-07-31 2018-03-20 バジル・エム・ハンタッシュＢａｓｉｌ Ｍ． ＨＡＮＴＡＳＨ Ｈｌａｇ改変された細胞および方法
US20140178988A1 (en) 2012-10-08 2014-06-26 Biotime, Inc. Differentiated Progeny of Clonal Progenitor Cell Lines
AU2013248265B2 (en) 2012-11-08 2018-11-01 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
SG10201707811XA (en) 2012-12-31 2017-11-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
MX2015008577A (es) 2012-12-31 2015-09-07 Janssen Biotech Inc Cultivo de celulas madre embrionarias humanas en la interfase aire-liquido para la diferenciacion en celulas endocrinas pancreaticas.
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
EP4039798A1 (en) 2012-12-31 2022-08-10 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
EP3498824A1 (en) 2013-04-26 2019-06-19 Memorial Sloan-Kettering Cancer Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
EP3003290B1 (en) 2013-06-05 2021-03-10 AgeX Therapeutics, Inc. Compositions for use in the treatment of wounds in mammalian species
WO2014197934A1 (en) 2013-06-14 2014-12-18 The University Of Queensland Renal progenitor cells
CA2931334A1 (en) 2013-11-21 2015-05-28 Memorial Sloan-Kettering Cancer Center Specification of functional cranial placode derivatives from human pluripotent stem cells
US11078462B2 (en) 2014-02-18 2021-08-03 ReCyte Therapeutics, Inc. Perivascular stromal cells from primate pluripotent stem cells
KR102162138B1 (ko) 2014-05-16 2020-10-06 얀센 바이오테크 인코포레이티드 췌장 내분비 세포에서 mafa 발현을 향상시키기 위한 소분자의 용도
US20170114323A1 (en) 2014-06-19 2017-04-27 Whitehead Institute For Biomedical Research Uses of kinase inhibitors for inducing and maintaining pluripotency
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells
CN104357379B (zh) * 2014-09-30 2017-08-08 刘兴宇 干细胞培养基
JP2018536438A (ja) 2015-12-07 2018-12-13 バイオタイム，インコーポレーテッド 多様な多能性幹細胞から誘導された褐色脂肪細胞の再誘導のための方法
EP3423158B1 (en) 2016-02-24 2023-11-15 The Rockefeller University Embryonic cell-based therapeutic candidate screening systems, models for huntington's disease and uses thereof
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
KR101877793B1 (ko) * 2016-07-15 2018-07-13 주식회사 엔바이오텍 줄기세포 배양용 무혈청 배지 조성물 및 이를 이용한 줄기세포의 배양 방법
JP2019537729A (ja) 2016-09-28 2019-12-26 オルガノボ インコーポレイテッド アッセイにおける人工腎臓組織の使用
CN106754652B (zh) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754657B (zh) * 2017-03-28 2022-07-22 北京赛斯达生物技术有限公司 一种猴胚胎干细胞的无血清培养基
US20210123016A1 (en) 2018-05-02 2021-04-29 Novartis Ag Regulators of human pluripotent stem cells and uses thereof
CN114109294A (zh) * 2020-08-26 2022-03-01 中石化胜利石油工程有限公司管具技术服务中心 闸板拆装装置及其控制系统

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US670372A (en) * 1898-01-13 1901-03-19 William D Carpenter Process of producing casein products.
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5750376A (en) * 1991-07-08 1998-05-12 Neurospheres Holdings Ltd. In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5612211A (en) 1990-06-08 1997-03-18 New York University Stimulation, production and culturing of hematopoietic progenitor cells by fibroblast growth factors
US5639046A (en) * 1992-07-21 1997-06-17 Fabio Perini S.P.A. Machine and method for the formation of coreless logs of web material
US5690926A (en) 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU3392697A (en) 1996-06-14 1998-01-07 Regents Of The University Of California, The (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
US6331406B1 (en) * 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
GB9722370D0 (en) * 1997-10-22 1997-12-17 Ici Plc Dye sheet cassette and printing apparatus
EP1025204A4 (en) 1997-10-23 2001-02-28 Geron Corp Methods and materials for the growth of primate-derived primordial stem cells
DE19756864C5 (de) 1997-12-19 2014-07-10 Oliver Brüstle Neurale Vorläuferzellen, Verfahren zu ihrer Herstellung und ihre Verwendung zur Therapie von neuralen Defekten
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
EP1179046B1 (en) 1999-05-07 2006-03-08 University Of Utah Research Foundation Lineage-restricted precursor cells isolated from mouse neural tube and mouse embryonic stem cells
IL129966A (en) * 1999-05-14 2009-12-24 Technion Res & Dev Foundation ISOLATED HUMAN EMBRYOID BODIES (hEB) DERIVED FROM HUMAN EMBRYONIC STEM CELLS
US6750581B2 (en) * 2002-01-24 2004-06-15 Visteon Global Technologies, Inc. Automotive alternator stator assembly with rectangular continuous wire

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US670372A (en) * 1898-01-13 1901-03-19 William D Carpenter Process of producing casein products.
US5750376A (en) * 1991-07-08 1998-05-12 Neurospheres Holdings Ltd. In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells

Also Published As

Publication number Publication date
US20050148070A1 (en) 2005-07-07
EP1261691B1 (en) 2013-07-31
NO20024200D0 (no) 2002-09-03
JP5839666B2 (ja) 2016-01-06
US7005252B1 (en) 2006-02-28
CA2402299A1 (en) 2001-09-13
NO335780B1 (no) 2015-02-16
EP1261691A2 (en) 2002-12-04
IS6515A (is) 2002-08-20
AU4197301A (en) 2001-09-17
JP5717311B2 (ja) 2015-05-13
HK1053616A1 (zh) 2003-10-31
JP2003525625A (ja) 2003-09-02
KR100795760B1 (ko) 2008-01-21
IL151270A0 (en) 2003-04-10
US7217569B2 (en) 2007-05-15
NO20024200L (no) 2002-09-03
US20030190748A1 (en) 2003-10-09
WO2001066697A2 (en) 2001-09-13
CA2402299C (en) 2012-12-18
MXPA02008698A (es) 2003-04-14
WO2001066697A3 (en) 2002-03-07
BR0108507A (pt) 2002-12-17
NZ520701A (en) 2004-03-26
CN1416345A (zh) 2003-05-07
AU2001241973B2 (en) 2006-11-09
JP2012005489A (ja) 2012-01-12
KR20030032926A (ko) 2003-04-26
US20060040384A1 (en) 2006-02-23
IL151270A (en) 2008-07-08
JP2011234735A (ja) 2011-11-24

Similar Documents

Publication Publication Date Title
CN100372928C (zh) 灵长类动物胚胎干细胞的无血清培养物
KR101437927B1 (ko) 영장류 배아 줄기 세포의 배양
JP5227318B2 (ja) 細胞増殖培地
AU2001241973A1 (en) Serum free cultivation of primate embryonic stem cells
US20080241919A1 (en) Defined media for pluripotent stem cell culture
AU2007200575B2 (en) Serum free cultivation of primate embryonic stem cells
HK1053616B (en) Serum free cultivation of primate embryonic stem cells
JP6446496B2 (ja) 霊長類胚性幹細胞の培養

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1053616

Country of ref document: HK

CX01 Expiry of patent term

Granted publication date: 20080305

CX01 Expiry of patent term