KR100538669B1 - 리간드 쌍의 결합 검출에 사용되는 화합물, 이를 포함하는조성물 및 키트 - Google Patents

리간드 쌍의 결합 검출에 사용되는 화합물, 이를 포함하는조성물 및 키트 Download PDF

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Publication number
KR100538669B1
KR100538669B1 KR10-2004-7013511A KR20047013511A KR100538669B1 KR 100538669 B1 KR100538669 B1 KR 100538669B1 KR 20047013511 A KR20047013511 A KR 20047013511A KR 100538669 B1 KR100538669 B1 KR 100538669B1
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acid
formula
tag
group
moi
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Korean (ko)
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KR20040081811A (ko
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반네스제프리
테이본존씨
하우버트제이제프리
멀리건존티
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퀴아진 지노믹스, 인코포레이티드
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Publication of KR20040081811A publication Critical patent/KR20040081811A/ko
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/01—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C233/45—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C07C233/46—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/167—Mass label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/627—Detection means characterised by use of a special device being a mass spectrometer

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Luminescent Compositions (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Hydrogenated Pyridines (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
KR10-2004-7013511A 1996-01-23 1997-01-23 리간드 쌍의 결합 검출에 사용되는 화합물, 이를 포함하는조성물 및 키트 Expired - Fee Related KR100538669B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US1043696P 1996-01-23 1996-01-23
US60/010,436 1996-01-23
US1540296P 1996-03-21 1996-03-21
US60/015,402 1996-03-21
PCT/US1997/001070 WO1997027327A2 (en) 1996-01-23 1997-01-23 Methods and compositions for detecting binding of ligand pair using non-fluorescent label

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR10-1998-0705636A Division KR100473903B1 (ko) 1996-01-23 1997-01-23 리간드쌍의결합을검출하는방법

Publications (2)

Publication Number Publication Date
KR20040081811A KR20040081811A (ko) 2004-09-22
KR100538669B1 true KR100538669B1 (ko) 2005-12-26

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KR10-2004-7013511A Expired - Fee Related KR100538669B1 (ko) 1996-01-23 1997-01-23 리간드 쌍의 결합 검출에 사용되는 화합물, 이를 포함하는조성물 및 키트
KR10-1998-0705636A Expired - Fee Related KR100473903B1 (ko) 1996-01-23 1997-01-23 리간드쌍의결합을검출하는방법

Family Applications After (1)

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KR10-1998-0705636A Expired - Fee Related KR100473903B1 (ko) 1996-01-23 1997-01-23 리간드쌍의결합을검출하는방법

Country Status (17)

Country Link
EP (1) EP0850320B1 (https=)
JP (2) JP2000507091A (https=)
KR (2) KR100538669B1 (https=)
CN (2) CN1515541A (https=)
AT (1) ATE187501T1 (https=)
AU (1) AU717330B2 (https=)
BR (1) BR9707060A (https=)
CA (1) CA2243989A1 (https=)
CZ (1) CZ218398A3 (https=)
DE (1) DE69700902T2 (https=)
ES (1) ES2143848T3 (https=)
GR (1) GR3032843T3 (https=)
HU (1) HUP9900459A3 (https=)
NZ (1) NZ331042A (https=)
PL (1) PL328272A1 (https=)
PT (1) PT850320E (https=)
WO (1) WO1997027327A2 (https=)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
DK0840804T3 (da) * 1996-01-23 2000-07-17 Rapigene Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyremolekyler ved anvendelse af størrelsesseparationsteknikker
AU2069597A (en) * 1996-03-04 1997-09-22 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
EP0963443B1 (en) * 1996-12-10 2006-03-08 Sequenom, Inc. Releasable nonvolatile mass-label molecules
AU6186398A (en) * 1997-02-24 1998-09-09 Tm Technologies, Inc. Process for selecting anti-sense oligonucleotides
ATE335845T1 (de) * 1997-05-16 2006-09-15 Exact Sciences Corp Elektrophoretische analyse von molekülen mit immobilisierten sonden
CA2297158C (en) * 1997-07-22 2009-12-22 Rapigene Inc. Methods and compounds for analyzing nucleic acids by mass spectrometry
EP1003914A1 (en) * 1997-08-05 2000-05-31 Ambion, Inc. Methods and compositions for stripping nucleic acids
US6365731B1 (en) 1997-08-06 2002-04-02 Ambion, Inc. Stripping nucleic acids with iodine and sodium thiosulfate
US5968822A (en) * 1997-09-02 1999-10-19 Pecker; Iris Polynucleotide encoding a polypeptide having heparanase activity and expression of same in transduced cells
AU1384299A (en) 1997-11-06 1999-05-31 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
GB9823646D0 (en) 1997-12-19 1998-12-23 Brax Genomics Ltd Compounds for mass spectrometry
GB9815166D0 (en) * 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds for mass spectrometry
GB9815164D0 (en) * 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds for mass spectrometry
GB9815163D0 (en) * 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds
US6104028A (en) 1998-05-29 2000-08-15 Genetrace Systems Inc. Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry
GB9812596D0 (en) * 1998-06-11 1998-08-12 Amersham Pharm Biotech Uk Ltd Energy transfer assay method
WO2000020643A1 (en) 1998-10-05 2000-04-13 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
KR20020037042A (ko) * 1999-08-31 2002-05-17 퀴아젠 지노믹스 인크. 광절단가능한 결합(들)을 갖는 에난티오머적으로-농축된화합물 및 이와 관련된 방법
DE19945351C2 (de) * 1999-09-22 2002-04-18 Lohmann Therapie Syst Lts Verfahren zum Auffinden und zur Isolierung pharmakologisch wirksamer Verbindungen aus Substanzgemischen
CA2398107C (en) 2000-01-28 2013-11-19 Althea Technologies, Inc. Methods for analysis of gene expression
US7537938B2 (en) 2000-04-28 2009-05-26 Monogram Biosciences, Inc. Biomarker detection in circulating cells
US7771929B2 (en) 2000-04-28 2010-08-10 Monogram Biosciences, Inc. Tag library compounds, compositions, kits and methods of use
US7160735B2 (en) 2000-04-28 2007-01-09 Monogram Biosciences, Inc. Tagged microparticle compositions and methods
US6716634B1 (en) 2000-05-31 2004-04-06 Agilent Technologies, Inc. Increasing ionization efficiency in mass spectrometry
US6548252B1 (en) 2000-10-13 2003-04-15 Esa, Inc. Detection of DNA damage
CA2445053A1 (en) 2001-05-21 2002-11-28 Aclara Biosciences, Inc. Methods and compositions for analyzing proteins
US7041459B2 (en) 2001-05-21 2006-05-09 Monogram Biosciences, Inc. Analyzing phosphorylated proteins
WO2002097112A2 (en) 2001-05-26 2002-12-05 Aclara Biosciences, Inc. Catalytic amplification of multiplexed assay signals
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20040229294A1 (en) 2002-05-21 2004-11-18 Po-Ying Chan-Hui ErbB surface receptor complexes as biomarkers
US7402397B2 (en) 2002-05-21 2008-07-22 Monogram Biosciences, Inc. Detecting and profiling molecular complexes
AU2003242605A1 (en) * 2002-06-03 2003-12-19 Pamgene B.V. Novel high density arrays and methods for analyte analysis
GB0306756D0 (en) * 2003-03-24 2003-04-30 Xzillion Gmbh & Co Kg Mass labels
US7250388B2 (en) * 2003-11-07 2007-07-31 Hewlett-Packard Development Company, L.P. Medium for chromatographic separations
FR2868067B1 (fr) * 2004-03-23 2006-06-23 Neomps Sa Derives d'amino-acides utiles comme agents de liaison a un support solide, leur procede de preparation et leur application a lasynthese de peptides
JP2008514900A (ja) 2004-07-30 2008-05-08 アデザ・バイオメデイカル・コーポレイシヨン 疾病および他の状態のマーカーとしての癌胎児性フィブロネクチンならびに癌胎児性フィブロネクチンの検出のための方法
WO2007000669A2 (en) 2005-06-07 2007-01-04 Centre National De La Recherche Scientifique (Cnrs) Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections
GB0518585D0 (en) 2005-09-12 2005-10-19 Electrophoretics Ltd Mass labels
US7605916B2 (en) 2006-09-21 2009-10-20 Intel Corporation Online analyte detection by surface enhanced Raman scattering (SERS)
GB0704764D0 (en) 2007-03-12 2007-04-18 Electrophoretics Ltd Isobarically labelled reagents and methods of their use
GB0809488D0 (en) 2008-05-23 2008-07-02 Electrophoretics Ltd Mass spectrometric analysis
GB0811574D0 (en) * 2008-06-24 2008-07-30 Trillion Genomics Ltd Characterising planar samples by mass spectrometry
GB0905438D0 (en) * 2009-03-30 2009-05-13 Ge Healthcare Ltd Radiolabelling reagents and methods
US20100297778A1 (en) * 2009-05-20 2010-11-25 Abbott Laboratories Conjugate Having Cleavable Linking Agent
GB0921665D0 (en) 2009-12-10 2010-01-27 Trillion Genomics Ltd Phobes
ES2676183T3 (es) 2010-07-02 2018-07-17 Ventana Medical Systems, Inc. Detección de dianas usando marcas de masa y espectrometría de masas
EP2752668A3 (en) 2010-07-23 2014-10-15 Beckman Coulter, Inc. System Or Method Of Including Analytical Units
US9637773B2 (en) 2011-01-13 2017-05-02 Enzo Life Sciences, Inc. Compounds and methods for detection of enzymes that remove formyl, succinyl, methyl succinyl or myristoyl groups from ε-amino lysine moieties
KR20140091032A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 수송 시스템의 자기 감쇠
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
WO2013070755A2 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Centrifuge system and workflow
ES2778054T3 (es) 2011-11-07 2020-08-07 Beckman Coulter Inc Sistema y método para transportar recipientes de muestras
CN104040357B (zh) 2011-11-07 2016-11-23 贝克曼考尔特公司 等分器系统以及工作流
US9446418B2 (en) 2011-11-07 2016-09-20 Beckman Coulter, Inc. Robotic arm
US8927732B2 (en) * 2012-03-30 2015-01-06 General Electric Company Biotin stannane for HPLC-free radioiodination
GB201322567D0 (en) 2013-12-19 2014-02-05 Electrophoretics Ltd Mass labels
CN103792219A (zh) * 2014-02-27 2014-05-14 厦门大学 红肉蜜柚光谱鉴别法
CN108780072B (zh) * 2016-01-22 2021-11-05 普度研究基金会 带电质量标记系统
US10814319B2 (en) * 2016-09-30 2020-10-27 Board Of Regents, University Of Texas System Functionalized polyolefin capillaries for open tubular ion chromatography
CN108490093A (zh) * 2018-03-29 2018-09-04 曲阜师范大学 一种开关型硫醇荧光标记试剂的荧光检测应用及其合成方法
CN112240915B (zh) * 2019-07-19 2022-08-12 杭州盛弗泰新材料科技有限公司 一种5-氨基-1h-1，2，4-三氮唑-3-羧酸的高效液相色谱检测方法
WO2021092795A1 (zh) * 2019-11-13 2021-05-20 李峰 核酸检测方法及装置
US12240813B2 (en) 2020-05-19 2025-03-04 Cybin Irl Limited Deuterated tryptamine derivatives and methods of use
CN112816578B (zh) * 2020-12-30 2021-09-24 上海市农业科学院 一种含氨基小分子蘑菇毒素的检测方法以及一种试剂盒
CN112881491A (zh) * 2021-02-05 2021-06-01 深圳大学 一种生物传感器电极的制备方法和葡萄糖生物传感器
CN113548981B (zh) * 2021-08-05 2023-04-28 齐鲁工业大学 三苯胺酚类化合物及其制备方法与其检测色氨酸的应用
US20250059600A1 (en) * 2021-12-09 2025-02-20 Roche Molecular Systems, Inc. Mass based detection of pcr amplicons
CN116496335B (zh) * 2023-03-21 2024-03-08 中国药科大学 一种修饰核苷酸及其应用
CN116751361B (zh) * 2023-06-15 2026-03-31 邢珺月 一种非病毒基因载体及其制备方法和应用
CN117535045B (zh) * 2023-07-12 2024-05-31 中国热带农业科学院分析测试中心 一种痕量噻虫嗪检测用Zn-MOF@Au NPS/DNA适配体荧光探针及其制备方法
EP4667939A1 (en) 2024-06-21 2025-12-24 Commissariat à l'Energie Atomique et aux Energies Alternatives Multiplex cancer cell profiling using ms-tagged probes containing an iminosydnone cleavable linker

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4709016A (en) * 1982-02-01 1987-11-24 Northeastern University Molecular analytical release tags and their use in chemical analysis
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
FI80476C (fi) * 1987-10-09 1990-06-11 Orion Yhtymae Oy Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning.
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK220890D0 (da) * 1990-09-14 1990-09-14 Ole Buchardt Fremgangsmaade til fremstilling af c-terminalt amiderede peptider
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
JPH0723800A (ja) * 1993-07-08 1995-01-27 Tanabe Seiyaku Co Ltd 核酸の検出方法
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
DE69701671T3 (de) * 1996-01-23 2006-08-17 Qiagen Genomics, Inc., Bothell Verfahren und zusammenstellungen zur sequenzbestimmung von nukleinsäuremolekülen
DK0840804T3 (da) * 1996-01-23 2000-07-17 Rapigene Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyremolekyler ved anvendelse af størrelsesseparationsteknikker

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Publication number Publication date
CZ218398A3 (cs) 1998-12-16
PT850320E (pt) 2000-05-31
EP0850320B1 (en) 1999-12-08
PL328272A1 (en) 1999-01-18
DE69700902T2 (de) 2000-07-20
WO1997027327A3 (en) 1997-11-20
BR9707060A (pt) 1999-12-28
KR19990081923A (ko) 1999-11-15
JP2000507091A (ja) 2000-06-13
CN1159452C (zh) 2004-07-28
ES2143848T3 (es) 2000-05-16
JP2008220370A (ja) 2008-09-25
HUP9900459A2 (hu) 1999-06-28
AU1707997A (en) 1997-08-20
CN1515541A (zh) 2004-07-28
CA2243989A1 (en) 1997-07-31
DE69700902D1 (de) 2000-01-13
KR100473903B1 (ko) 2005-09-30
HUP9900459A3 (en) 1999-11-29
GR3032843T3 (en) 2000-07-31
KR20040081811A (ko) 2004-09-22
ATE187501T1 (de) 1999-12-15
NZ331042A (en) 1999-10-28
CN1212019A (zh) 1999-03-24
AU717330B2 (en) 2000-03-23
EP0850320A2 (en) 1998-07-01
WO1997027327A2 (en) 1997-07-31

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