JPWO2020004490A5 - - Google Patents

Info

Publication number

JPWO2020004490A5

JPWO2020004490A5 JP2020527592A JP2020527592A JPWO2020004490A5 JP WO2020004490 A5 JPWO2020004490 A5 JP WO2020004490A5 JP 2020527592 A JP2020527592 A JP 2020527592A JP 2020527592 A JP2020527592 A JP 2020527592A JP WO2020004490 A5 JPWO2020004490 A5 JP WO2020004490A5

Authority

JP

Japan

Prior art keywords

amino acid

seq

antibody

acid sequence

acid sequences

Prior art date

2019-06-26

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 54
• 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 54
• 230000004927 fusion Effects 0.000 claims description 34
• 108010047041 Complementarity Determining Regions Proteins 0.000 claims description 24
• 239000000203 mixture Substances 0.000 claims description 17
• 101710173787 Chondroitin sulfate proteoglycan 5 Proteins 0.000 claims description 15
• 102100028758 Chondroitin sulfate proteoglycan 5 Human genes 0.000 claims description 15
• 210000004556 brain Anatomy 0.000 claims description 13
• 239000000427 antigen Substances 0.000 claims description 10
• 102000036639 antigens Human genes 0.000 claims description 10
• 108091007433 antigens Proteins 0.000 claims description 10
• 210000004027 cell Anatomy 0.000 claims description 7
• 238000000034 method Methods 0.000 claims description 7
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• 239000012634 fragment Substances 0.000 claims description 4
• 230000014759 maintenance of location Effects 0.000 claims description 4
• 102000039446 nucleic acids Human genes 0.000 claims description 4
• 108020004707 nucleic acids Proteins 0.000 claims description 4
• 150000007523 nucleic acids Chemical class 0.000 claims description 4
• 208000014644 Brain disease Diseases 0.000 claims description 3
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 2
• BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 claims description 2
• 210000001130 astrocyte Anatomy 0.000 claims description 2
• 229920001477 hydrophilic polymer Polymers 0.000 claims description 2
• 229920002521 macromolecule Polymers 0.000 claims description 2
• 238000004519 manufacturing process Methods 0.000 claims description 2
• 210000002569 neuron Anatomy 0.000 claims description 2
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
• 125000003275 alpha amino acid group Chemical group 0.000 claims 124
• 238000012258 culturing Methods 0.000 claims 1
• 150000001413 amino acids Chemical group 0.000 description 254
• 108010076504 Protein Sorting Signals Proteins 0.000 description 80
• 239000002773 nucleotide Substances 0.000 description 37
• 125000003729 nucleotide group Chemical group 0.000 description 36
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 4
• 101100168771 Mus musculus Cspg5 gene Proteins 0.000 description 3
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• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
• 235000002198 Annona diversifolia Nutrition 0.000 description 1
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• LTXREWYXXSTFRX-QGZVFWFLSA-N Linagliptin Chemical compound N=1C=2N(C)C(=O)N(CC=3N=C4C=CC=CC4=C(C)N=3)C(=O)C=2N(CC#CC)C=1N1CCC[C@@H](N)C1 LTXREWYXXSTFRX-QGZVFWFLSA-N 0.000 description 1
• 241000282560 Macaca mulatta Species 0.000 description 1
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• NFLRWRCXIJABMY-UHFFFAOYSA-M cesium diaminomethylideneazanium 2,2,2-trifluoroacetate thiocyanate Chemical compound [Cs+].[S-]C#N.NC(N)=N.OC(=O)C(F)(F)F NFLRWRCXIJABMY-UHFFFAOYSA-M 0.000 description 1
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• BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
• 229910000397 disodium phosphate Inorganic materials 0.000 description 1
• 235000019800 disodium phosphate Nutrition 0.000 description 1
• 239000012153 distilled water Substances 0.000 description 1
• 239000007758 minimum essential medium Substances 0.000 description 1
• 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
• 235000019796 monopotassium phosphate Nutrition 0.000 description 1
• 239000013642 negative control Substances 0.000 description 1
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• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
• 239000010452 phosphate Substances 0.000 description 1
• 239000008363 phosphate buffer Substances 0.000 description 1
• 239000002504 physiological saline solution Substances 0.000 description 1
• GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
• LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
• 102000004196 processed proteins & peptides Human genes 0.000 description 1
• 150000003839 salts Chemical class 0.000 description 1
• 239000001488 sodium phosphate Substances 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1