JPS63246667A - 糞便中のヘモグロビンの検出方法 - Google Patents
糞便中のヘモグロビンの検出方法Info
- Publication number
- JPS63246667A JPS63246667A JP8047087A JP8047087A JPS63246667A JP S63246667 A JPS63246667 A JP S63246667A JP 8047087 A JP8047087 A JP 8047087A JP 8047087 A JP8047087 A JP 8047087A JP S63246667 A JPS63246667 A JP S63246667A
- Authority
- JP
- Japan
- Prior art keywords
- hemoglobin
- feces
- sample
- spoon
- excrement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000001554 Hemoglobins Human genes 0.000 title claims abstract description 47
- 108010054147 Hemoglobins Proteins 0.000 title claims abstract description 47
- 238000001514 detection method Methods 0.000 title description 9
- 108010014251 Muramidase Proteins 0.000 claims abstract description 10
- 102000016943 Muramidase Human genes 0.000 claims abstract description 10
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 10
- 229960000274 lysozyme Drugs 0.000 claims abstract description 10
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 10
- 239000004325 lysozyme Substances 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 229940088598 enzyme Drugs 0.000 claims abstract description 7
- 210000003608 fece Anatomy 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 27
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 claims description 15
- 238000003018 immunoassay Methods 0.000 claims description 4
- 230000001420 bacteriolytic effect Effects 0.000 claims description 2
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 11
- 230000001900 immune effect Effects 0.000 abstract description 9
- 229920001817 Agar Polymers 0.000 abstract description 8
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract description 8
- 239000000758 substrate Substances 0.000 abstract description 5
- 239000008272 agar Substances 0.000 abstract description 3
- 238000004040 coloring Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 3
- CMPQUABWPXYYSH-UHFFFAOYSA-N phenyl phosphate Chemical compound OP(O)(=O)OC1=CC=CC=C1 CMPQUABWPXYYSH-UHFFFAOYSA-N 0.000 abstract description 2
- 238000005070 sampling Methods 0.000 abstract 2
- 102000005744 Glycoside Hydrolases Human genes 0.000 abstract 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 9
- 230000000968 intestinal effect Effects 0.000 description 9
- 230000002550 fecal effect Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 210000003097 mucus Anatomy 0.000 description 6
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- 230000000740 bleeding effect Effects 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 108091007734 digestive enzymes Proteins 0.000 description 3
- 102000038379 digestive enzymes Human genes 0.000 description 3
- 210000002249 digestive system Anatomy 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 102000013415 peroxidase activity proteins Human genes 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 210000005095 gastrointestinal system Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8047087A JPS63246667A (ja) | 1987-03-31 | 1987-03-31 | 糞便中のヘモグロビンの検出方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8047087A JPS63246667A (ja) | 1987-03-31 | 1987-03-31 | 糞便中のヘモグロビンの検出方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63246667A true JPS63246667A (ja) | 1988-10-13 |
| JPH0569466B2 JPH0569466B2 (enrdf_load_stackoverflow) | 1993-10-01 |
Family
ID=13719148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8047087A Granted JPS63246667A (ja) | 1987-03-31 | 1987-03-31 | 糞便中のヘモグロビンの検出方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63246667A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0259466U (enrdf_load_stackoverflow) * | 1988-10-20 | 1990-05-01 | ||
| JPH0260870U (enrdf_load_stackoverflow) * | 1988-10-26 | 1990-05-07 | ||
| WO2019168109A1 (ja) | 2018-03-02 | 2019-09-06 | 栄研化学株式会社 | 検体中に含まれる蛋白質を安定化する方法、検体中に含まれる蛋白質を安定化させるための溶液 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI841542B (zh) | 2017-12-01 | 2024-05-11 | 日商榮研化學股份有限公司 | 將血紅素與血紅素結合球蛋白之複合體安定化之方法、及用以保存含有血紅素之檢體之保存溶液 |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52125623A (en) * | 1976-04-13 | 1977-10-21 | Chugai Pharmaceut Co Ltd | Quantitative determination of antigenic substances and reagent kit for the same |
| JPS54157690A (en) * | 1976-07-06 | 1979-12-12 | Becton Dickinson Co | Plastic substrate coated with acceptor for ligand to be detected* and method of making said substrate |
| JPS56106154A (en) * | 1980-01-17 | 1981-08-24 | Suovaniemi Finnpipette | Detection method of hemoglobin in night soil |
| JPS5823796A (ja) * | 1981-07-16 | 1983-02-12 | エフ．ホフマン ― ラ ロシュ アーゲー | 人潜血の検出方法 |
| JPS59125064A (ja) * | 1983-01-05 | 1984-07-19 | Eiken Kagaku Kk | 免疫学的ラテツクス凝集反応をもちいた糞便中のヘモグロビンの検出方法および該方法に使用する試薬 |
| JPS59182367A (ja) * | 1983-02-02 | 1984-10-17 | オ−ストレイリアン・モノクロ−ナル・デベロプメント・プロプライエタリ−・リミテツド | 糞便中の微量血液を検出するための方法および診断器具 |
| JPS61228351A (ja) * | 1985-04-02 | 1986-10-11 | Kyoto Ikagaku Kenkyusho:Kk | 糞便中のヘモグロビンの検出方法 |
| JPS6261584A (ja) * | 1985-09-13 | 1987-03-18 | Tdk Corp | 生理活性物質固定化磁気微粒子 |
-
1987
- 1987-03-31 JP JP8047087A patent/JPS63246667A/ja active Granted
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52125623A (en) * | 1976-04-13 | 1977-10-21 | Chugai Pharmaceut Co Ltd | Quantitative determination of antigenic substances and reagent kit for the same |
| JPS54157690A (en) * | 1976-07-06 | 1979-12-12 | Becton Dickinson Co | Plastic substrate coated with acceptor for ligand to be detected* and method of making said substrate |
| JPS56106154A (en) * | 1980-01-17 | 1981-08-24 | Suovaniemi Finnpipette | Detection method of hemoglobin in night soil |
| JPS5823796A (ja) * | 1981-07-16 | 1983-02-12 | エフ．ホフマン ― ラ ロシュ アーゲー | 人潜血の検出方法 |
| JPS59125064A (ja) * | 1983-01-05 | 1984-07-19 | Eiken Kagaku Kk | 免疫学的ラテツクス凝集反応をもちいた糞便中のヘモグロビンの検出方法および該方法に使用する試薬 |
| JPS59182367A (ja) * | 1983-02-02 | 1984-10-17 | オ−ストレイリアン・モノクロ−ナル・デベロプメント・プロプライエタリ−・リミテツド | 糞便中の微量血液を検出するための方法および診断器具 |
| JPS61228351A (ja) * | 1985-04-02 | 1986-10-11 | Kyoto Ikagaku Kenkyusho:Kk | 糞便中のヘモグロビンの検出方法 |
| JPS6261584A (ja) * | 1985-09-13 | 1987-03-18 | Tdk Corp | 生理活性物質固定化磁気微粒子 |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0259466U (enrdf_load_stackoverflow) * | 1988-10-20 | 1990-05-01 | ||
| JPH0260870U (enrdf_load_stackoverflow) * | 1988-10-26 | 1990-05-07 | ||
| WO2019168109A1 (ja) | 2018-03-02 | 2019-09-06 | 栄研化学株式会社 | 検体中に含まれる蛋白質を安定化する方法、検体中に含まれる蛋白質を安定化させるための溶液 |
| KR20200128122A (ko) | 2018-03-02 | 2020-11-11 | 에이껜 가가꾸 가부시끼가이샤 | 검체 중에 포함된 단백질을 안정화시키는 방법, 검체 중에 포함된 단백질을 안정화시키기 위한 용액 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0569466B2 (enrdf_load_stackoverflow) | 1993-10-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |