JPS62153758A - フロ−サイトメ−タによる網状赤血球測定方法 - Google Patents
フロ−サイトメ−タによる網状赤血球測定方法Info
- Publication number
- JPS62153758A JPS62153758A JP60293700A JP29370085A JPS62153758A JP S62153758 A JPS62153758 A JP S62153758A JP 60293700 A JP60293700 A JP 60293700A JP 29370085 A JP29370085 A JP 29370085A JP S62153758 A JPS62153758 A JP S62153758A
- Authority
- JP
- Japan
- Prior art keywords
- light
- fluorescence
- exciting
- reticulocytes
- optical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001995 reticulocyte Anatomy 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims description 16
- 230000003287 optical effect Effects 0.000 claims abstract description 32
- 210000004027 cell Anatomy 0.000 claims abstract description 17
- 210000003743 erythrocyte Anatomy 0.000 claims description 13
- 230000005284 excitation Effects 0.000 claims description 12
- 210000001772 blood platelet Anatomy 0.000 claims description 8
- 230000000638 stimulation Effects 0.000 claims description 7
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 2
- 210000000601 blood cell Anatomy 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 7
- 238000007796 conventional method Methods 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000004159 blood analysis Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000005375 photometry Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- YDSPVZISFWHZIL-UHFFFAOYSA-N 9-(4-aminophenyl)-7-methylacridin-3-amine;nitrate Chemical compound [O-][N+]([O-])=O.C12=CC(C)=CC=C2N=C2C=C(N)C=CC2=C1C1=CC=C(N)C=C1 YDSPVZISFWHZIL-UHFFFAOYSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000009610 hypersensitivity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000004298 light response Effects 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
- G01N2015/014—Reticulocytes
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60293700A JPS62153758A (ja) | 1985-12-27 | 1985-12-27 | フロ−サイトメ−タによる網状赤血球測定方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60293700A JPS62153758A (ja) | 1985-12-27 | 1985-12-27 | フロ−サイトメ−タによる網状赤血球測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62153758A true JPS62153758A (ja) | 1987-07-08 |
| JPH0464590B2 JPH0464590B2 (zh) | 1992-10-15 |
Family
ID=17798106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60293700A Granted JPS62153758A (ja) | 1985-12-27 | 1985-12-27 | フロ−サイトメ−タによる網状赤血球測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62153758A (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0767382A3 (en) * | 1995-10-06 | 1998-02-25 | Toa Medical Electronics Co., Ltd. | Fluorescent compounds and their use for measuring reticulocytes |
| EP0806664A3 (en) * | 1996-04-12 | 1998-04-08 | Toa Medical Electronics Co., Ltd. | A reagent for measuring reticulocytes and a method of measuring them |
| US20210033592A1 (en) * | 2018-04-28 | 2021-02-04 | Shenzhen Mindray Bio-Medical Electronics Co. | Blood analyzer and analysis method |
-
1985
- 1985-12-27 JP JP60293700A patent/JPS62153758A/ja active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0767382A3 (en) * | 1995-10-06 | 1998-02-25 | Toa Medical Electronics Co., Ltd. | Fluorescent compounds and their use for measuring reticulocytes |
| EP0806664A3 (en) * | 1996-04-12 | 1998-04-08 | Toa Medical Electronics Co., Ltd. | A reagent for measuring reticulocytes and a method of measuring them |
| US20210033592A1 (en) * | 2018-04-28 | 2021-02-04 | Shenzhen Mindray Bio-Medical Electronics Co. | Blood analyzer and analysis method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0464590B2 (zh) | 1992-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |