JPH1194742A - 蛍光顕微鏡 - Google Patents
蛍光顕微鏡Info
- Publication number
- JPH1194742A JPH1194742A JP25113297A JP25113297A JPH1194742A JP H1194742 A JPH1194742 A JP H1194742A JP 25113297 A JP25113297 A JP 25113297A JP 25113297 A JP25113297 A JP 25113297A JP H1194742 A JPH1194742 A JP H1194742A
- Authority
- JP
- Japan
- Prior art keywords
- light
- excitation
- wavelength
- light amount
- excitation light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005284 excitation Effects 0.000 claims description 167
- 230000003287 optical effect Effects 0.000 claims description 88
- 238000005259 measurement Methods 0.000 claims description 19
- 230000001678 irradiating effect Effects 0.000 abstract description 14
- 238000002834 transmittance Methods 0.000 description 36
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 18
- 238000010586 diagram Methods 0.000 description 16
- 239000007850 fluorescent dye Substances 0.000 description 12
- 238000005286 illumination Methods 0.000 description 10
- 238000003384 imaging method Methods 0.000 description 8
- 238000005562 fading Methods 0.000 description 7
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 7
- 210000001747 pupil Anatomy 0.000 description 6
- 230000005540 biological transmission Effects 0.000 description 5
- 238000010226 confocal imaging Methods 0.000 description 5
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 5
- 229910052753 mercury Inorganic materials 0.000 description 5
- 230000004888 barrier function Effects 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25113297A JPH1194742A (ja) | 1997-09-16 | 1997-09-16 | 蛍光顕微鏡 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25113297A JPH1194742A (ja) | 1997-09-16 | 1997-09-16 | 蛍光顕微鏡 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH1194742A true JPH1194742A (ja) | 1999-04-09 |
| JPH1194742A5 JPH1194742A5 (enExample) | 2005-06-02 |
Family
ID=17218157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP25113297A Pending JPH1194742A (ja) | 1997-09-16 | 1997-09-16 | 蛍光顕微鏡 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH1194742A (enExample) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001235683A (ja) * | 2000-01-27 | 2001-08-31 | Leica Microsystems Heidelberg Gmbh | 顕微鏡組立物 |
| JP2002139675A (ja) * | 2000-10-31 | 2002-05-17 | Olympus Optical Co Ltd | レーザ顕微鏡 |
| JP2005140981A (ja) * | 2003-11-06 | 2005-06-02 | Nikon Corp | 顕微鏡装置 |
| JP2005352030A (ja) * | 2004-06-09 | 2005-12-22 | Olympus Corp | 走査型レーザ観察装置、観察方法及び観察プログラム |
| JP2007500880A (ja) * | 2003-05-16 | 2007-01-18 | ユニヴェルシテイト ファン アムステルダム | 対象物の画像を形成する方法及び装置 |
| JP2008249669A (ja) * | 2007-03-30 | 2008-10-16 | Osaka Prefecture Univ | 物質・物体の認識・識別方法および装置、蛍光顕微鏡、ヘッドアップディスプレイ装置 |
| JP2012255286A (ja) * | 2011-06-08 | 2012-12-27 | Topcon Corp | 建設機械制御システム |
| CN110208187A (zh) * | 2019-04-29 | 2019-09-06 | 吉林亚泰中科医疗器械工程技术研究院股份有限公司 | 一种用于滤光片和光纤自动同步更换的装置 |
| CN113167647A (zh) * | 2018-10-22 | 2021-07-23 | 罗伯特·博世有限公司 | 用于芯片实验室分析仪器的光学模块的滤波设备、用于芯片实验室分析仪器的光学模块和用于运行芯片实验室分析仪器的光学模块的方法 |
-
1997
- 1997-09-16 JP JP25113297A patent/JPH1194742A/ja active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001235683A (ja) * | 2000-01-27 | 2001-08-31 | Leica Microsystems Heidelberg Gmbh | 顕微鏡組立物 |
| JP2002139675A (ja) * | 2000-10-31 | 2002-05-17 | Olympus Optical Co Ltd | レーザ顕微鏡 |
| JP2007500880A (ja) * | 2003-05-16 | 2007-01-18 | ユニヴェルシテイト ファン アムステルダム | 対象物の画像を形成する方法及び装置 |
| JP2005140981A (ja) * | 2003-11-06 | 2005-06-02 | Nikon Corp | 顕微鏡装置 |
| JP2005352030A (ja) * | 2004-06-09 | 2005-12-22 | Olympus Corp | 走査型レーザ観察装置、観察方法及び観察プログラム |
| JP2008249669A (ja) * | 2007-03-30 | 2008-10-16 | Osaka Prefecture Univ | 物質・物体の認識・識別方法および装置、蛍光顕微鏡、ヘッドアップディスプレイ装置 |
| JP2012255286A (ja) * | 2011-06-08 | 2012-12-27 | Topcon Corp | 建設機械制御システム |
| CN113167647A (zh) * | 2018-10-22 | 2021-07-23 | 罗伯特·博世有限公司 | 用于芯片实验室分析仪器的光学模块的滤波设备、用于芯片实验室分析仪器的光学模块和用于运行芯片实验室分析仪器的光学模块的方法 |
| JP2022508894A (ja) * | 2018-10-22 | 2022-01-19 | ローベルト ボツシユ ゲゼルシヤフト ミツト ベシユレンクテル ハフツング | ラボオンチップ分析装置用の光学モジュールのためのフィルタ装置、ラボオンチップ分析装置用の光学モジュール、及び、ラボオンチップ分析装置用の光学モジュールを動作させるための方法 |
| US11988605B2 (en) | 2018-10-22 | 2024-05-21 | Robert Bosch Gmbh | Filter device for an optical module for a lab-on-a-chip analysis device, optical module for a lab-on-a-chip analysis device and method for operating an optical module for a lab-on-a-chip analysis device |
| CN110208187A (zh) * | 2019-04-29 | 2019-09-06 | 吉林亚泰中科医疗器械工程技术研究院股份有限公司 | 一种用于滤光片和光纤自动同步更换的装置 |
| CN110208187B (zh) * | 2019-04-29 | 2024-03-19 | 吉林亚泰中科医疗器械工程技术研究院股份有限公司 | 一种用于滤光片和光纤自动同步更换的装置 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Written amendment |
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| A621 | Written request for application examination |
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