JPH10510718A - 血管内皮増殖因子−b - Google Patents
血管内皮増殖因子−bInfo
- Publication number
- JPH10510718A JPH10510718A JP8526450A JP52645096A JPH10510718A JP H10510718 A JPH10510718 A JP H10510718A JP 8526450 A JP8526450 A JP 8526450A JP 52645096 A JP52645096 A JP 52645096A JP H10510718 A JPH10510718 A JP H10510718A
- Authority
- JP
- Japan
- Prior art keywords
- vegf
- protein
- acid sequence
- amino acid
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 特徴的なアミノ酸配列 Pro−Xaa−Cys−Val−Xaa−Xaa−Xaa−Arg−Cys− Xaa−Gly−Cys−Cys(配列認識番号16) を有し、内皮細胞又は中胚葉細胞の増殖を促進する特性を有するたん白質をコー ドする単離核酸であって、 前記核酸は、図1および2のDNA(配列認識番号1)と、図3のDNA(配列 認識番号4)と、図5のDNA(配列認識番号6)と、図7のDNA(配列認識 番号8)と、図10のDNA(配列認識番号10)と、図12のDNA(配列認 識番号12)と、図14のDNA(配列認識番号14)と、前記DNA配列の少 なくとも一つとストリンジェントな条件下でハイブリダズする核酸とから成るグ ループから選択される単離核酸。 2. 前記核酸はcDNAである請求項1の核酸。 3. 図1および2のDNA(配列認識番号1)に対応するcDNAを有する請 求項1の核酸。 4. 核酸配列が哺乳類DNAである請求項1の核酸。 5. 前記核酸はマウスDNAである請求項4の核酸。 6. 前記核酸はヒトDNAである請求項4の核酸。 7. 前記核酸は血管内皮細胞の増殖を促進するたん白質をコードする請求項1 の核酸。 8. 図3のDNA(配列認識番号4)に対応するcDNAを有する請求 項1の核酸。 9. 図5のDNA(配列認識番号6)に対応するcDNAを有する請求項1の 核酸。 10.図7のDNA(配列認識番号8)に対応するcDNAを有する請求項1の 核酸。 11.図10のDNA(配列認識番号10)に対応するcDNAを有する請求項 1の核酸。 12.図12のDNA(配列認識番号12)に対応するcDNAを有する請求項 1の核酸。 13.図14のDNA(配列認識番号14)に対応するcDNAを有する請求項 1の核酸。 14.プロモーター配列と操作可能にリンクされた請求項1の核酸を有するベク ター。 15.前記ベクターは真核ベクターである請求項14のベクター。 16.前記ベクターは原核ベクターである請求項14のベクター。 17.前記ベクターはプラスミドである請求項14のベクター。 18.特徴的な配列 Pro−Xaa−Cys−Val−Xaa−Xaa−Xaa−Arg−Cys− Xaa−Gly−Cys−Cys(配列認識番号16) を示し、内皮細胞又は中胚葉細胞の増殖を促進する特性を有する単離たん白質で あって、前記たん白質は、図1のアミノ酸配列(配列認識番号2)と、図2のア ミノ酸配列(配列認識番号3)と、図4のアミノ酸配列(配列認識番号5)と、 図6のアミノ酸配列(配列認識番号7)と、図8のアミノ酸配列(配列認識番号 9)と、図11のアミノ酸配列(配列認識番号11)と、図13のアミノ酸配列 (配列認識番号13)と、図15のアミノ酸配列(配列認識番号15)とから成 るグループから選択されるアミノ酸配列に実質的に対応するアミノ酸配列を有す る単離たん白質。 19.前記たん白質は、図1のアミノ酸配列(配列認識番号2)に対応するアミ ノ酸配列を有する請求項18の単離たん白質。 20.前記たん白質は、図2のアミノ酸配列(配列認識番号3)に対応するアミ ノ酸配列を有する請求項18の単離たん白質。 21.前記たん白質は、図4のアミノ酸配列(配列認識番号5)に対応するアミ ノ酸配列を有する請求項18の単離たん白質。 22.前記たん白質は、図6のアミノ酸配列(配列認識番号7)に対応するアミ ノ酸配列を有する請求項18の単離たん白質。 23.前記たん白質は、図8のアミノ酸配列(配列認識番号9)に対応するアミ ノ酸配列を有する請求項18の単離たん白質。 24.前記たん白質は、図11のアミノ酸配列(配列認識番号11)に対応する アミノ酸配列を有する請求項18の単離たん白質。 25.前記たん白質は、図13のアミノ酸配列(配列認識番号13)に対応する アミノ酸配列を有する請求項18の単離たん白質。 26.前記たん白質は、図15のアミノ酸配列(配列認識番号15)に対応する アミノ酸配列を有する請求項18の単離たん白質。 27.前記たん白質は哺乳類たん白質である請求項18の単離たん白質。 28.前記たん白質はマウスたん白質である請求項27の単離たん白質。 29.前記たん白質はヒトたん白質である請求項27の単離たん白質。 30.前記たん白質は血管内皮細胞の増殖を促進する請求項18の単離たん白質 。 31.図1および2のDNA(配列認識番号1)と、図3のDNA(配列認識番 号4)と、図5のDNA(配列認識番号6)と、図7のDNA(配列認識番号8 )と、図10のDNA(配列認識番号10)と、図12のDNA(配列認識番号 12)と、図14のDNA(配列認識番号14)と、前記DNA配列の少なくと も一つとストリンジェントな条件下でハイブリダイズするDNAとから成るグル ープから選択されるDNAの発現によって作り出される単離たん白質。 32.内皮細胞又は中胚葉細胞の増殖を促進するのに有効な量の請求項18のた ん白質と、少なくとも一つの従来式製薬用キャリア又は希釈剤とを有する医薬組 成物。 33.請求項18のたん白質と反応する抗体。 34.前記抗体はモノクローナル抗体である請求項33の抗体。 35.請求項14のベクターによってトランスフォーメーション又はトランスフ ェクションされて、内皮細胞又は中胚葉細胞の増殖を促進する特徴を有するたん 白質を発現する宿主細胞。 36.前記宿主細胞は真核細胞である請求項35のトランスフェクションされた 宿主細胞。 37.前記宿主細胞はCOS細胞である請求項35のトランスフェクションされ た宿主細胞。 38.前記宿主細胞は原核細胞である請求項35のトランスフェクションされた 宿主細胞。 39.前記宿主細胞は293EBNA細胞である請求項35のトランスフェクシ ョンされた宿主細胞。 40.前記宿主細胞は昆虫細胞である請求項35のトランスフェクションされた 宿主細胞。 41.サンプル中のVEGF−Bの量を検出するために、VEGF−Bと反応す る請求項33の抗体を有する、テストサンプル中のVEGF−Bを定量的に検出 するための診断手段。 42.前記抗体は標識化抗体である請求項41の診断手段。 43.テストVEGF−B遺伝子と請求項1の核酸配列との配列比較を容易にす るために、ポリメラーゼ連鎖反応によってテストVEGF−B遺伝子を増幅する ために、請求項1の核酸配列に対して相補的な少なくとも一対のプライマーを有 する、テストサンプル中のVEGF−Bを検出するた めの診断手段。 44.有効VEGF−B結合量の請求項33の抗体と、少なくとも一つの薬学的 に許容可能なキャリア又はアジュバントと、を有する医薬組成物。 45.内皮細胞又は中胚葉細胞の増殖を促進するのに有効な量の請求項18のた ん白質と、VEGFとを有する医薬組成物。 46.更に、細胞増殖促進量の前記たん白質が、細胞と結合しない状態で存在す ることを確実にするのに有効な量のヘパリンを有する請求項45の医薬組成物。 47.更に、細胞増殖促進量の前記たん白質が、細胞と結合しない状態で存在す ることを確実にするのに有効な量のヘパリンを有する請求項32の医薬組成物。 48.血管新生の刺激に有効な量の請求項18のたん白質と、ヘパリンとを有す る医薬組成物。 49.請求項18のたん白質を有する単離たん白質ダイマー。 50.前記たん白質ダイマーは前記たん白質のホモダイマーである請求項49の 単離たん白質ダイマー。 51.前記たん白質ダイマーは前記たん白質とVEGFとのヘテロダイマーであ る請求項49の単離たん白質ダイマー。 52.前記たん白質ダイマーはジスルフィド−結合ダイマーである請求項49の 単離たん白質ダイマー。 53.請求項18のたん白質と、 VEGFから成るグループから選択される少 なくとも一つのたん白質を、その少なくとも1つのたん白質を発現する細胞から 放出させるのを促進する方法であって、前記細胞をヘパリンに対して露出する工 程を有する方法。 54.請求項1の核酸配列の少なくとも一部に対して相補的なアンチセンスヌク レオチド配列を有するベクター。 55.VEGF−Bを発現する細胞からのVEGF−Bの発現を遅延させる方法 であって、前記細胞に請求項54のベクターをトランスフェクションする工程を 有する方法。 56.前記細胞は腫瘍細胞である請求項55の方法。 57.図25の配列(配列認識番号17)に対応するヌクレオチド配列、又は、 前記核酸配列とストリンジェントな条件下でハイブリダイズする核酸を有する単 離核酸。 58.VEGF−Bたん白質を発現するべく、適当なプロモーターに操作可能に リンクされた、請求項1の核酸配列を有するベクターでトランスフォーメーショ ン又はトランスフェクションされた宿主細胞。 59.前記VEGF−Bたん白質は、図1のアミノ酸配列(配列認識番号2)と 、図2のアミノ酸配列(配列認識番号3)と、図4のアミノ酸配列(配列認識番 号5)と、図6のアミノ酸配列(配列認識番号7)と、図8のアミノ酸配列(配 列認識番号9)と、図11のアミノ酸配列(配列認識番号11)と、図13のア ミノ酸配列(配列認識番号13)と、図15のアミノ酸配列(配列認識番号15 )とから成るグループから選択されるア ミノ酸配列に実質的に対応するアミノ酸配列からなる請求項58の宿主細胞。 60.請求項1の核酸配列に対して相補的な少なくとも一対のプライマーを有す るテストサンプル中のVEGF−Bを検出するための診断手段。 61.請求項18のたん白質とVEGFから成るグループから選択される少なく とも1つのたん白質を、前記少なくとも一つのたん白質を発現する細胞から得る ための方法であって、前記細胞をヘパリンに対して露出して、前記少なくとも1 つのたん白質の放出を誘発させる工程と、放出されたたん白質を収集する工程、 とを有する方法。 62.請求項1のヌクレオチド配列を、適当なプロモーターと操作可能にリンク された関係で、ベクターに導入する工程を有するVEGF−Bたん白質の発現に 適したベクターを作る方法。 63.前記単離核酸分子は、50%ホルムアミド、5xSSC pH7.0又は 5xSSPE緩衝液、1%ないし2%のSDS、5ないし10xデンハルト溶液 、そして、100μg/mlの鮭精子DNA中に於いて、42℃で、マウスVE GF−Bをコードする核酸分子とハイブリダイズするヒトVEGF−B分子をコ ードする単離核酸分子。
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US569,063 | 1990-08-17 | ||
US39765195A | 1995-03-01 | 1995-03-01 | |
US08/397,651 | 1995-03-01 | ||
US08/469,427 | 1995-06-06 | ||
US08/469,427 US5607918A (en) | 1995-03-01 | 1995-06-06 | Vascular endothelial growth factor-B and DNA coding therefor |
US08/569,063 | 1995-12-06 | ||
US08/569,063 US5928939A (en) | 1995-03-01 | 1995-12-06 | Vascular endothelial growth factor-b and dna coding therefor |
US469,427 | 1995-12-06 | ||
US397,651 | 1995-12-06 | ||
PCT/US1996/002957 WO1996026736A1 (en) | 1995-03-01 | 1996-03-01 | Vascular endothelial growth factor-b |
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PT814827E (pt) | 2003-11-28 |
US5840693A (en) | 1998-11-24 |
AU741856B2 (en) | 2001-12-13 |
US6331301B1 (en) | 2001-12-18 |
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EP0814827A1 (en) | 1998-01-07 |
US20030170253A1 (en) | 2003-09-11 |
JP3297687B2 (ja) | 2002-07-02 |
EP0814827A4 (en) | 1999-11-10 |
CN1176603A (zh) | 1998-03-18 |
CN1146440C (zh) | 2004-04-21 |
DE69629181D1 (de) | 2003-08-28 |
JP3275024B2 (ja) | 2002-04-15 |
US5928939A (en) | 1999-07-27 |
AU5302196A (en) | 1996-09-18 |
DK0814827T3 (da) | 2003-11-10 |
CA2211687C (en) | 2011-05-24 |
HK1008485A1 (en) | 1999-07-30 |
DE69629181T2 (de) | 2004-05-13 |
ATE245437T1 (de) | 2003-08-15 |
EP0814827B1 (en) | 2003-07-23 |
NO973933L (no) | 1997-09-01 |
NZ305091A (en) | 1998-11-25 |
WO1996026736A1 (en) | 1996-09-06 |
NO973933D0 (no) | 1997-08-27 |
CA2211687A1 (en) | 1996-09-06 |
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