JPH09511404A - 呼吸器多核体ウイルスプロテインgのペプチド断片、免疫原的薬剤、それを含む医薬組成物、および製造法 - Google Patents
呼吸器多核体ウイルスプロテインgのペプチド断片、免疫原的薬剤、それを含む医薬組成物、および製造法Info
- Publication number
- JPH09511404A JPH09511404A JP7526123A JP52612395A JPH09511404A JP H09511404 A JPH09511404 A JP H09511404A JP 7526123 A JP7526123 A JP 7526123A JP 52612395 A JP52612395 A JP 52612395A JP H09511404 A JPH09511404 A JP H09511404A
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. サブグループAおよびサブグループBのヒト呼吸器多核体ウイルス、ま たはウシ呼吸器多核体ウイルス、またはウシのGタンパク質の配列のアミノ酸残 基130と230との間のペプチド配列、または前記ペプチド配列と少なくとも 80%の相同性を有する配列を保持することを特徴とする、免疫原要素として使 用できるポリペプチド。 2. RSVプロテインGのアミノ酸残基番号174と187との間のペプチ ド配列または対応する配列と少なくとも80%の相同性を有する配列を含むこと を特徴とする、請求項1に記載のポリペプチド。 3. a)位置173および/または186におけるCysアミノ酸がジスル フィド架橋を形成しないアミノ酸、特にセリンにより置換されている、および/ または b)位置176および182におけるアミノ酸がジスルフィド架橋以外の共有 結合の架橋を形成することができるアミノ酸、特にアスパラギン酸およびオルニ チンである、 配列を含むことを特徴とする、請求項1または2に記載のポリペプチド。 4. RSVプロテインGの配列のアミノ酸残基番号140と200の間との ペプチド配列または前記ペプチド配列と少なくとも80%の相同性を有する配列 から成ることを特徴とする、請求項1〜3のいずれか一項に記載のポリペプチド 。 5. RSVプロテインGの配列のアミノ酸残基番号158と190との間の ペプチド配列または前記ペプチド配列と少なくとも80%の相同性を有する配列 から成ることを特徴とする、請求項1〜3のいずれか一項に記載のポリペプチド 。 6. ヒトRSV、サブグループAおよびサブグループBのプロテインGまた はウシRSVの配列のアミノ酸残基番号130と230との間のペプチド配列ま たは前記ペプチド配列と少なくとも80%の相同性を有する配列から成ることを 特徴とする、請求項1〜3のいずれか一項に記載のポリペプチド。 7. 下記の配列: 配列番号5: Ser Ile Cys Ser Asn Asn Pro Thr Cys Trp Ala Ile Cys Lys、 配列番号6: Ser Ile Cys Gly Asn Asn Gln Leu Cys Lys Ser Ile Cys Lys、 配列番号7: Ser Ile Cys Ser Asn Asn Pro Thr Cys Trp Ala Ile Ser Lys、 配列番号8: Ser Ile Cys Gly Asn Asn Gln Leu Cys Lys Ser Ile Ser Lys、 配列番号9: Ser Ile Asp Ser Asn Asn Pro Thr Orn Trp Ala Ile Cys Lys、 配列番号10: Ser Ile Asp Gly Asn Asn Gln Leu Orn Lys Ser Ile Cys Lys、 配列番号11: Ser Ile Asp Ser Asn Asn Pro Thr Orn Trp Ala Ile Ser Lys、 配列番号12: Ser Ile Asp Gly Asn Asn Gln Leu Orn Lys Ser Ile Ser Lys、 の中の1つを有することを特徴とする、請求項1〜3のいずれか一項に記載のポ リペプチド。 8. 配列番号14〜73の中の1つを有することを特徴とする、請求項1〜 3のいずれか一項に記載のポリペプチド。 9. N−末端またはC−末端の位置に少なくとも1つのシステイン残基をさ らに含むことを特徴とする、請求項1〜8のいずれか一項に記載のポリペプチド 。 10. 担体タンパク質に結合した請求項1〜9のいずれか一項に記載のポリ ペプチドを含むことを特徴とする免疫原的薬剤。 11. 担体タンパク質が免疫アジュバントタンパク質であることを特徴とす る、請求項10に記載の免疫原的薬剤。 12. 担体タンパク質がOmpAタンパク質であることを特徴とする、請求 項10および11に記載の免疫原的薬剤。 13. ポリペプチドがクレブシエラ(Klebsiella)属の細菌の外膜のタンパク 質との可溶性複合体の形態であることを特徴とする、請求項10〜12のいずれ か一項に記載の薬剤。 14. 免疫アジュバントタンパク質がクレブシエラ・ニューモニエ(Klebsie lla pneumoniae)のタンパク質p40またはタンパク質p40と少なくとも80 %の相同性を有するタンパク質であることを特徴とする、請求項11〜13のい ずれか一項に記載の薬剤。 15. ポリペプチドが結合タンパク質により担体タンパク質に複合化されて いることを特徴とする、請求項10〜14のいずれか一項に記載の薬剤。 16. 結合タンパク質がヒト血清アルブミンレセプターであることを特徴と する、請求項15に記載の薬剤。 17. 前記ポリペプチドが配列番号13を含むタンパク質に結合されている ことを特徴とする、請求項10〜16のいずれか一項に記載の薬剤。 18. 結合が共有結合であることを特徴とする、請求項10〜17のいずれ か一項に記載の薬剤。 19. 生物学的経路により得られることを特徴とする、請求項10〜18の いずれか一項に記載の薬剤。 20. 請求項1〜9のいずれか一項に記載のポリペプチドまたは請求項10 〜19のいずれか一項に記載の薬剤を含有することを特徴とする、ヒトRSV、 サブグループAおよび/またはサブグループB、またはウシRSVにより誘発さ れる感染の予防および/または治療用組成物。 21. 注射可能な経路による投与に適合した薬学上許容される賦形剤をさら に含有することを特徴とする、請求項20に記載の組成物。 22. 非特異的免疫アジュバントを含むことを特徴とする、請求項20また は21に記載の組成物。 23. 請求項1〜9のいずれか一項に記載のポリペプチドをコードすること を特徴とするヌクレオチド配列。 24. 配列番号13を含むタンパク質をコードすることを特徴とするヌクレ オチド配列。 25. a)クレブシエラ(Klebsiella)属の細菌の膜リポ多糖類を2価のカチ オンおよび界面活性剤の存在下に沈降させて、上清中に全体の膜タンパク質を回 収し、 b)前記タンパク質をアニオン交換クロマトグラフィーにかけて、免疫アジュ バントタンパク質を含有する画分を分離し、 c)免疫アジュバントタンパク質を含有する画分を濃縮し、 d)免疫アジュバントタンパク質を請求項1〜9のいずれか一項に記載のポリ ペプチドと複合化させて可溶性複合体を形成する、 ことを特徴とする、請求項20または21に記載の組成物の中に挿入された複合 化ペプチドを製造する方法。 26. 工程d)を0.05%より低いか、またはそれに等しい濃度のグルタ ルアルデヒドの存在下に5日より長いか、またはそれに等しい期間の間実施する ことを特徴とする、請求項25に記載の方法。 27. 抗原の免疫原性を改良するための、配列番号13または少なくとも8 0%の相同性を有する配列を有するタンパク質の使用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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FR9404009A FR2718452B1 (fr) | 1994-04-06 | 1994-04-06 | Elément d'immunogène, agent immunogène, composition pharmaceutique et procédé de préparation. |
FR94/04009 | 1994-04-06 | ||
PCT/FR1995/000444 WO1995027787A1 (fr) | 1994-04-06 | 1995-04-06 | Fragment peptidique de la proteine g du virus respiratoire syncytial, agent immunogene, composition pharmaceutique le contenant et procede de preparation |
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JP2005221425A Division JP4180590B2 (ja) | 1994-04-06 | 2005-07-29 | クレブシエラ・ニューモニエ由来の免疫アジュバントタンパク質 |
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JPH09511404A true JPH09511404A (ja) | 1997-11-18 |
JP3883569B2 JP3883569B2 (ja) | 2007-02-21 |
Family
ID=9461767
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JP52612395A Expired - Fee Related JP3883569B2 (ja) | 1994-04-06 | 1995-04-06 | 呼吸器多核体ウイルスプロテインgのペプチド断片、免疫原的薬剤、それを含む医薬組成物、および製造法 |
JP2005221425A Expired - Fee Related JP4180590B2 (ja) | 1994-04-06 | 2005-07-29 | クレブシエラ・ニューモニエ由来の免疫アジュバントタンパク質 |
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JP2005221425A Expired - Fee Related JP4180590B2 (ja) | 1994-04-06 | 2005-07-29 | クレブシエラ・ニューモニエ由来の免疫アジュバントタンパク質 |
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US (5) | US6113911A (ja) |
EP (2) | EP0754231B1 (ja) |
JP (2) | JP3883569B2 (ja) |
AT (1) | ATE331031T1 (ja) |
AU (1) | AU708856B2 (ja) |
BR (1) | BR1100321A (ja) |
CA (2) | CA2187083C (ja) |
DE (1) | DE69535074T2 (ja) |
DK (1) | DK0754231T3 (ja) |
ES (1) | ES2268691T3 (ja) |
FR (1) | FR2718452B1 (ja) |
NZ (2) | NZ284500A (ja) |
PT (1) | PT754231E (ja) |
WO (1) | WO1995027787A1 (ja) |
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1994
- 1994-04-06 FR FR9404009A patent/FR2718452B1/fr not_active Expired - Fee Related
-
1995
- 1995-04-06 PT PT95916721T patent/PT754231E/pt unknown
- 1995-04-06 US US08/721,979 patent/US6113911A/en not_active Expired - Fee Related
- 1995-04-06 ES ES95916721T patent/ES2268691T3/es not_active Expired - Lifetime
- 1995-04-06 NZ NZ284500A patent/NZ284500A/en not_active IP Right Cessation
- 1995-04-06 JP JP52612395A patent/JP3883569B2/ja not_active Expired - Fee Related
- 1995-04-06 DK DK95916721T patent/DK0754231T3/da active
- 1995-04-06 EP EP95916721A patent/EP0754231B1/fr not_active Expired - Lifetime
- 1995-04-06 WO PCT/FR1995/000444 patent/WO1995027787A1/fr active IP Right Grant
- 1995-04-06 CA CA2187083A patent/CA2187083C/fr not_active Expired - Fee Related
- 1995-04-06 EP EP00126606A patent/EP1111053A3/fr not_active Withdrawn
- 1995-04-06 DE DE69535074T patent/DE69535074T2/de not_active Expired - Lifetime
- 1995-04-06 AT AT95916721T patent/ATE331031T1/de active
- 1995-04-06 CA CA002635998A patent/CA2635998A1/fr not_active Abandoned
- 1995-04-06 NZ NZ329833A patent/NZ329833A/en not_active IP Right Cessation
- 1995-04-06 AU AU23109/95A patent/AU708856B2/en not_active Ceased
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1997
- 1997-04-22 BR BR1100321A patent/BR1100321A/pt active IP Right Grant
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2000
- 2000-06-30 US US09/582,876 patent/US6537556B1/en not_active Expired - Fee Related
- 2000-09-01 US US09/654,289 patent/US6410030B1/en not_active Expired - Fee Related
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2002
- 2002-03-05 US US10/091,257 patent/US6616930B2/en not_active Expired - Fee Related
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2003
- 2003-09-05 US US10/655,855 patent/US7220545B2/en not_active Expired - Fee Related
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Also Published As
Publication number | Publication date |
---|---|
ES2268691T3 (es) | 2007-03-16 |
EP0754231A1 (fr) | 1997-01-22 |
US6537556B1 (en) | 2003-03-25 |
FR2718452A1 (fr) | 1995-10-13 |
AU2310995A (en) | 1995-10-30 |
DE69535074T2 (de) | 2007-06-28 |
US6616930B2 (en) | 2003-09-09 |
US6113911A (en) | 2000-09-05 |
US20050250933A1 (en) | 2005-11-10 |
JP3883569B2 (ja) | 2007-02-21 |
DE69535074D1 (de) | 2006-08-03 |
JP4180590B2 (ja) | 2008-11-12 |
ATE331031T1 (de) | 2006-07-15 |
CA2187083C (fr) | 2011-06-07 |
FR2718452B1 (fr) | 1996-06-28 |
BR1100321A (pt) | 2000-03-21 |
WO1995027787A1 (fr) | 1995-10-19 |
CA2187083A1 (fr) | 1995-10-19 |
EP1111053A3 (fr) | 2001-08-08 |
NZ329833A (en) | 2000-02-28 |
DK0754231T3 (da) | 2006-10-23 |
EP1111053A2 (fr) | 2001-06-27 |
JP2006020637A (ja) | 2006-01-26 |
AU708856B2 (en) | 1999-08-12 |
EP0754231B1 (fr) | 2006-06-21 |
PT754231E (pt) | 2006-11-30 |
US20030064078A1 (en) | 2003-04-03 |
CA2635998A1 (fr) | 1995-10-19 |
US6410030B1 (en) | 2002-06-25 |
US7220545B2 (en) | 2007-05-22 |
NZ284500A (en) | 1998-04-27 |
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