JPH0372085B2 - - Google Patents
Info
- Publication number
- JPH0372085B2 JPH0372085B2 JP58044316A JP4431683A JPH0372085B2 JP H0372085 B2 JPH0372085 B2 JP H0372085B2 JP 58044316 A JP58044316 A JP 58044316A JP 4431683 A JP4431683 A JP 4431683A JP H0372085 B2 JPH0372085 B2 JP H0372085B2
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- sulfuric acid
- acid reaction
- absorption spectrum
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000006243 chemical reaction Methods 0.000 claims description 101
- 239000000126 substance Substances 0.000 claims description 71
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 50
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 50
- 238000004519 manufacturing process Methods 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 41
- 238000011282 treatment Methods 0.000 claims description 34
- 235000018102 proteins Nutrition 0.000 claims description 33
- 102000004169 proteins and genes Human genes 0.000 claims description 33
- 108090000623 proteins and genes Proteins 0.000 claims description 33
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 30
- 241000209094 Oryza Species 0.000 claims description 30
- 235000007164 Oryza sativa Nutrition 0.000 claims description 30
- 235000009566 rice Nutrition 0.000 claims description 30
- 238000000862 absorption spectrum Methods 0.000 claims description 28
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 26
- 150000004676 glycans Chemical class 0.000 claims description 26
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 26
- 229920001282 polysaccharide Polymers 0.000 claims description 26
- 239000005017 polysaccharide Substances 0.000 claims description 26
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 24
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 19
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 18
- 239000002244 precipitate Substances 0.000 claims description 18
- 239000007864 aqueous solution Substances 0.000 claims description 17
- 239000012528 membrane Substances 0.000 claims description 15
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 14
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 claims description 14
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 238000002844 melting Methods 0.000 claims description 13
- 230000008018 melting Effects 0.000 claims description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 238000000502 dialysis Methods 0.000 claims description 12
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 12
- 235000010755 mineral Nutrition 0.000 claims description 12
- 239000011707 mineral Substances 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- -1 ligroin Chemical compound 0.000 claims description 10
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 9
- MYKOKMFESWKQRX-UHFFFAOYSA-N 10h-anthracen-9-one;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 MYKOKMFESWKQRX-UHFFFAOYSA-N 0.000 claims description 8
- 238000001460 carbon-13 nuclear magnetic resonance spectrum Methods 0.000 claims description 8
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 8
- 235000018417 cysteine Nutrition 0.000 claims description 8
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 claims description 8
- 239000003495 polar organic solvent Substances 0.000 claims description 8
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 7
- FNEHAOQZWPHONV-UHFFFAOYSA-N 9h-carbazole;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C3=CC=CC=C3NC2=C1 FNEHAOQZWPHONV-UHFFFAOYSA-N 0.000 claims description 7
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 7
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 7
- 239000000470 constituent Substances 0.000 claims description 7
- 230000003544 deproteinization Effects 0.000 claims description 7
- 239000011630 iodine Substances 0.000 claims description 7
- 229910052740 iodine Inorganic materials 0.000 claims description 7
- 230000007935 neutral effect Effects 0.000 claims description 7
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 claims description 7
- 238000005185 salting out Methods 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 claims description 6
- 208000035473 Communicable disease Diseases 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 238000003809 water extraction Methods 0.000 claims description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 4
- 239000002246 antineoplastic agent Substances 0.000 claims description 4
- 239000002955 immunomodulating agent Substances 0.000 claims description 4
- 229940121354 immunomodulator Drugs 0.000 claims description 4
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 235000002639 sodium chloride Nutrition 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 230000003449 preventive effect Effects 0.000 claims description 3
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 3
- 229940124597 therapeutic agent Drugs 0.000 claims description 3
- 238000010306 acid treatment Methods 0.000 claims description 2
- 239000001103 potassium chloride Substances 0.000 claims description 2
- 235000011164 potassium chloride Nutrition 0.000 claims description 2
- 230000002584 immunomodulator Effects 0.000 claims 1
- 239000000843 powder Substances 0.000 description 25
- 241000699670 Mus sp. Species 0.000 description 24
- 206010028980 Neoplasm Diseases 0.000 description 20
- 239000006228 supernatant Substances 0.000 description 20
- 238000005119 centrifugation Methods 0.000 description 17
- 239000000243 solution Substances 0.000 description 16
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 14
- 230000000694 effects Effects 0.000 description 14
- 208000015181 infectious disease Diseases 0.000 description 13
- 229940088598 enzyme Drugs 0.000 description 12
- 239000002504 physiological saline solution Substances 0.000 description 12
- 241000699666 Mus <mouse, genus> Species 0.000 description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 10
- 108090000637 alpha-Amylases Proteins 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 8
- 102000004139 alpha-Amylases Human genes 0.000 description 8
- 229940024171 alpha-amylase Drugs 0.000 description 8
- 201000011510 cancer Diseases 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 6
- 230000000259 anti-tumor effect Effects 0.000 description 6
- 230000002519 immonomodulatory effect Effects 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 230000007123 defense Effects 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 239000011800 void material Substances 0.000 description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
- 230000024932 T cell mediated immunity Effects 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000002523 gelfiltration Methods 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 241000186779 Listeria monocytogenes Species 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 208000006268 Sarcoma 180 Diseases 0.000 description 3
- 206010070834 Sensitisation Diseases 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229960000583 acetic acid Drugs 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 210000002540 macrophage Anatomy 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000008313 sensitization Effects 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000007059 acute toxicity Effects 0.000 description 2
- 231100000403 acute toxicity Toxicity 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000006806 disease prevention Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 230000004727 humoral immunity Effects 0.000 description 2
- 230000016784 immunoglobulin production Effects 0.000 description 2
- 230000004957 immunoregulator effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000003801 milling Methods 0.000 description 2
- 150000007522 mineralic acids Chemical class 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- RFCLPRUJLQKYFX-BZNPZCIMSA-N (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxy-2,3,4-trimethylhexanal Chemical compound C[C@@](C=O)(O)[C@@](O)([C@](O)([C@H](O)CO)C)C RFCLPRUJLQKYFX-BZNPZCIMSA-N 0.000 description 1
- XJFIPJXYYUYQIC-VZFHVOOUSA-N (2R,3S,4S,5S)-2,3,4,5,6-pentahydroxy-6-methylheptanal Chemical compound CC([C@H]([C@H]([C@@H]([C@H](C=O)O)O)O)O)(O)C XJFIPJXYYUYQIC-VZFHVOOUSA-N 0.000 description 1
- CLINFZQBXPTCNW-FVGYRXGTSA-N (2s)-2-amino-3-(1h-indol-3-yl)propanoic acid;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 CLINFZQBXPTCNW-FVGYRXGTSA-N 0.000 description 1
- HULXDCYFWWGJQO-UTINFBMNSA-N (3R,4S,5R,6R)-3,4,5,6,7-pentahydroxy-3,4,5-trimethylheptan-2-one Chemical compound C[C@@]([C@@]([C@](C(=O)C)(O)C)(O)C)(O)[C@H](O)CO HULXDCYFWWGJQO-UTINFBMNSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- KPZGRMZPZLOPBS-UHFFFAOYSA-N 1,3-dichloro-2,2-bis(chloromethyl)propane Chemical compound ClCC(CCl)(CCl)CCl KPZGRMZPZLOPBS-UHFFFAOYSA-N 0.000 description 1
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- LCSKNASZPVZHEG-UHFFFAOYSA-N 3,6-dimethyl-1,4-dioxane-2,5-dione;1,4-dioxane-2,5-dione Chemical group O=C1COC(=O)CO1.CC1OC(=O)C(C)OC1=O LCSKNASZPVZHEG-UHFFFAOYSA-N 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 229920000298 Cellophane Polymers 0.000 description 1
- 241000272194 Ciconiiformes Species 0.000 description 1
- 208000027932 Collagen disease Diseases 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 239000001263 FEMA 3042 Substances 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 108010028688 Isoamylase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010059712 Pronase Proteins 0.000 description 1
- 235000019774 Rice Bran oil Nutrition 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 239000003957 anion exchange resin Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000001099 axilla Anatomy 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- AYJRCSIUFZENHW-DEQYMQKBSA-L barium(2+);oxomethanediolate Chemical compound [Ba+2].[O-][14C]([O-])=O AYJRCSIUFZENHW-DEQYMQKBSA-L 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000010335 hydrothermal treatment Methods 0.000 description 1
- 230000005934 immune activation Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 239000008165 rice bran oil Substances 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00Â -Â C08B35/00; Derivatives thereof
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
æ¬çºæã¯ç±³ç³ ãç±æ°Žæœåºããæœåºç©ãããã«é€
èçœæäœããããšã«ããåŸãããäžèšã®æ§è³ªãæ
ããæ°èŠå€ç³äœRDPç©è³ªããã®è£œé æ³ããã³ã
ããæå¹æåãšããæè «çå€ãå ç«èª¿ç¯å€ããã³
ææçäºé²æ²»çå€ã«é¢ãã詳ããã¯åªããæè «ç
掻æ§ãå ç«èª¿ç¯æŽ»æ§ããã³ææé²åŸ¡æŽ»æ§ãæãã
æ°èŠå€ç³äœRDPç©è³ªãšãç±³ç³ ãåæãšããŠåœè©²
ç©è³ªãæœåºã粟補ããåœè©²ç©è³ªã®è£œé æ³ã«é¢ã
ãããªããRDPãšã¯Rice bran De Proteinã®é
æåããšã€ãç¥èªã§ãããç±³ç³ ããè±èçœããã
ã®ãšããæå³ã§ããã (1) ååéïŒéæèãééãããååéïŒäžä»¥äž
ãšæšå®ãããã (2) 溶解æ§ïŒã¢ã«ã³ãŒã«ãã¢ã»ãã³ããããµã³ã
ãã³ãŒã³ãé ¢é žãšãã«ããžã¡ãã«ã¹ã«ãã
ã·ãããªã°ãã€ã³ãåå¡©åççŽ ãã¯ããã
ã«ã ããã³ãšãŒãã«ã«äžæº¶ã§ãããæ°Žã«ã¯
å¯æº¶ã§ããã (3) 氎溶液ã®PHïŒïŒïŒ 氎溶液ã¯äžæ§ã瀺ãã (4) èçœè³ªãç¡æ©è³ªïŒèçœè³ªãå«æãããïŒïŒ 以
äžã®ç¡æ©è³ªãå«æããã (5) åè²åå¿ïŒã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«
é žåå¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ã
ã€ã³ç¡«é žåå¿ãã¯ãã¢ãããŒãç¡«é žåå¿ã
ããšããŒã«ç¡«é žåå¿ãã«ã«ããŸãŒã«ç¡«é žå
å¿ãéœæ§ã§ãããããŠãŒã¬ããåå¿ããã³
ãããªã³åå¿ãããŒãªãŒããªãŒãªã³åå¿ã
ãšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ãé°æ§
ã§ããã (6) 玫å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã玫å€éš
åžåã¹ãã¯ãã«ã瀺ãã (7) èµ€å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºãèµ€å€éš
åžåã¹ãã¯ãã«ã瀺ãã (8) 13CâNMRã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã13Câ
NMRã¹ãã¯ãã«ã瀺ãã (9) æ§æç³ïŒã°ã«ã³ãŒã¹ã®ã¿ã§æ§æãããã (10) æ§é ïŒÎ±âïŒïŒïŒã°ã«ã³ã·ãçµåãäž»éªšæ Œãš
ããçŽ10åã®Î±âïŒïŒïŒçµåã§çµã°ããã°
ã«ã³ãŒã¹æ®åºã«ïŒåã®å²åã§Î±âïŒïŒïŒçµ
åãæããã (11) èç¹ïŒäžå®ã®èç¹ã瀺ããã220âã§è€å€ãã
280âã§é»å€ããŠçåãèµ·ããã ãã€ãŠãæ®»ç²ãè±ç²çãããã¯ãããã®è¡šå±€éš
ãåæãšããŠçç掻æ§ãæããç©è³ªã補é ããæ¹
æ³ãææ¡ãããŠãããããããã®æ¹æ³ã¯ååŸéã
éåžžã«å°ãªããæ¯æ§ãã¿ãããçã®åé¡ç¹ãã
ãã æ¬çºæã¯ãããã®åé¡ç¹ã解æ¶ãã¹ãéæç 究
ãç¶ããçµæãªããããã®ã§ããã æ¬çºæã®å€ç³äœRDPç©è³ªã¯ç±³ç³ ãç±æ°ŽåŠçã
ãŠåŸãããæœåºæ¶²ã«æ¥µæ§ææ©æº¶åªãå ããããã
ããã¯å¡©æå€ãå ããçããæ²ã§ããååããã®
ã¡æ°Žã«æº¶è§£ããé€èçœæäœãå¿ èŠã«å¿ããŠç²Ÿè£œåŠ
çãç²æ«åŠçãè¡ãªãããšã«ãã€ãŠè£œé ããããš
ãã§ããã æ¬çºæã«çšããããåæç±³ç³ ã¯éåžžã®ç²Ÿç±³ã«ã
ããŠçºçããç±³ç³ ã§ãããåœè©²ç±³ç³ ã®çºçæºã§ã
ãçç±³ã®åçš®ãç£å°ããã³ç²Ÿçœæ©ççãåããªã
ãããã®åæç±³ç³ ããã®å€ç³äœRDPç©è³ªã®æœåºã
粟補ã«å ç«ã€ãŠåœè©²åæäžã«æ··åšããç ç±³çã¯å¯
åçã«é€å»ããæŽæµããããšãæãŸããããŸãã
ç±³ç³ æ²¹ãæœåºããåŸã®æ®æž£ã§ããè±èç³ çã®ãã
ã«ãä»ã®ç®çã«äœ¿çšãããåŸã®ç±³ç³ ã§ãã€ãŠãæ¬
çºæã«äœ¿çšããããšãã§ããã ç±³ç³ ã®æœåºæ¶²ã¯ãç±³ç³ ãç±æ°ŽåŠçããããšã«ã
ãåŸããããç±æ°ŽåŠçã¯çŽ°ããç²ç ããç±³ç³ ã«å¯Ÿ
ãçŽïŒã10åéïŒééïŒã®èžçæ°ŽãŸãã¯ç²Ÿè£œæ°Žã
å ããã¹ãã³ã¬ã¹ã¿ã³ã¯ãããŒããŒåŒãã¿ã³ã¯ã
ã¬ã©ã¹è£œã¿ã³ã¯ãæµéåŒã®ç®¡ç¶æœåºè£ 眮çã®å®¹åš
ãããã¯ãããã®èå§æ§å®¹åšãçšããŠæ¹æãããŸ
ãã¯æ¹æããªãã§ïŒã15KgïŒcm2ã奜ãŸããã¯ïŒã
5.0KgïŒcm2ã®å§åã70ã200âã奜ãŸããã¯100ã
150âã®æž©åºŠã§10åä¹è³24æéã奜ãŸããã¯0.5ã
ïŒæéæ¹æããªããæœåºãè¡ãªããå®çšäžã¯ïŒã
3.0KgïŒcm2ã®å§åã100ã140âã®æž©åºŠã§ïŒãïŒæ
éã®ç±æ°ŽåŠçãé©åœã§ããã ãªãã該åŠçã«å ç«ã€ãŠãç±³ç³ ã¯æ°ŽæŽåŸãé ¢é ž
ãšãã«ãåå¡©åçé žãã¯ãããã«ã ããšãŒãã«ã
ïœâãããµã³ããã³ãŒã³ãç³æ²¹ãšãŒãã«ãã¢ã»ã
ã³çã®ææ©æº¶åªã«ãã€ãŠè溶æ§åºåã®é€å»ãè¡ãª
ãããšãæãŸããã åèšç±æ°ŽåŠçã«ããåŸãããæœåºæ¶²ã¯æ¿Ÿéãã
ãã¯é å¿åé¢çã®æäœã«ãã€ãŠåºåœ¢åãšåé¢ãã
å¿ èŠã«å¿ããŠé©åœãªæ段ãããšãã°æžå§æ¿çž®ãé
å€æ¿Ÿéçã®æ段ãåç¬ãããã¯çµåããŠè¡ãªãé©
åœéãŸã§æ¿çž®ããã ãã®æœåºæ¶²ã«å¯ŸããŠæ°Žã«å¯æº¶ãªæ¥µæ§ææ©æº¶åªã
ããã¯å¡©æå€ãå ããçããæ²ã§ããååããã
ãšã«ãã€ãŠå€ç³äœRDPç©è³ªãå«ãç»åãåŸãã
ãšãã§ãããããã§çšãã極æ§ææ©æº¶åªãšããŠ
ã¯ãããšãã°ã¡ã¿ããŒã«ããšã¿ããŒã«ããããã
ãŒã«ãã¢ã»ãã³çãããã極æ§ææ©æº¶åªã®äœ¿çšé
ã¯æœåºæ¶²äžã«ãããç®çç©è³ªã®å«æéçãèæ ®ã
ãŠæ±ºå®ãããããšã¿ããŒã«ãçšããå ŽåãäŸã«ã
ããšããšã¿ããŒã«æ¿åºŠã30ã50ïŒ ïŒïŒ¶ïŒïŒ¶ïŒã«ãª
ãããã«æ·»å ããã°ããããªããçããæ²ã§ãã¯
åèšãšã¿ããŒã«çã®ææ©æº¶åªã§æŽæµããããšã奜
ãŸãããäžæ¹ãå¡©æå€ãšããŠã¯å¡©åãããªãŠã ã
ç¡«é žã¢ã³ã¢ããŠã ãå¡©åã«ãªãŠã çããããéåžž
ã¯ç±æ°Žæœåºæ¶²ã«å¯Ÿãå¡©æå€ã飜å床0.5ãïŒã«ãª
ããŸã§å ããŠæ²ã§ããçæããããã æ¬çºæã§ã¯ã極æ§ææ©æº¶åªãããã¯å¡©æå€ãç±
æ°Žæœåºæ¶²ã«å ããåãããã¯äžèšã®åŠããããç©
質ãæ·»å ããŠæ²ã§ããçæãããã次ãã§æ°Žã«æº¶
解ããåŸã«ãé€èçœåŠçãå¿ èŠã«å¿ããŠç²Ÿè£œåŠç
ããã³ç²æ«ååŠçãåç¬ã§ãããã¯é©å®çµåããŠ
è¡ãªãããšãã§ãããé€èçœåŠçã粟補åŠçãšã
ãŠã¯çš®ã ã®æäœãé©çšããããšãã§ãããããšã
ã°å€ç³äœRDPç©è³ªãå«æãã溶液ã«æŸ±ç²åè§£é µ
çŽ ããã³ïŒãŸãã¯èçœåè§£é µçŽ ãäœçšãããŠæ··åš
ããŠãã柱ç²ãèçœè³ªãªã©ã®äžçŽç©ãäœåååã
ããäœåååããããã®ã¯åŸã®ç²Ÿè£œå·¥çšã§é€å»ã
ãããé µçŽ ã¯ä»»æã®ãã®ã䜿çšããããšãã§ãã
ããšãã°Î±âã¢ãã©ãŒãŒãã€ãœã¢ãã©ãŒãŒããã«
ã©ããŒãŒãªã©ã®çš®ã ã®ã¢ãã©ãŒãŒãããã€ã³ãã
ãã·ã³ãããªãã·ã³ãããããŒãŒãªã©ã®çš®ã ã®ã
ããã¢ãŒãŒãå¿ èŠãªãã°ä»ã®é µçŽ ãä»»æã«äœ¿çšã§
ãããé µçŽ åŠçã¯ã奜ãŸããã¯é µçŽ ãåºè³ªã®1/10
ïŒïŒã1/5000ã®å²åã§æ·»å ãã0.5ã24æéã奜ãŸ
ããã¯ïŒã15æéè¡ãªãã ä»ã®é€èçœåŠçããã³ç²Ÿè£œåŠçãšããŠãåèšå€
ç³äœRDPç©è³ªå«æ氎溶液ã«æ°·é ¢é žãç¡«é žãå¡©é žã
ã¿ã³ãã³é žãããªã¯ãã«é ¢é žãªã©ã®ç¡æ©é žããã
ã¯ææ©é žã0.1ã10ïŒ ã奜ãŸããã¯ïŒãïŒïŒ çšåºŠ
å ããæ²ã§ããçæãããªãã°æ¿Ÿéãé å¿åé¢ç
ã®æäœã«ããé€ããç¶ããŠæ®åããé žãç¡æ©ã€ãª
ã³ããã³äœåååºåãæµæ°ŽãŸãã¯èžçæ°Žäžã§ã»ã
ãã¢ã³èãã³ããžãªã³èçã®éæèã«ãŠïŒãïŒæ¥
éæããæ¹æ³ãããŠãšãã¯ã¹ãã¢ã³ããŒã©ã€ãã
ããŠãªã©ã€ãããã€ã€ã€ãªã³çã®ã«ããªã³ãã¢ã
ãªã³äº€ææš¹èãçšããã€ãªã³äº€æåŠçæ¹æ³ãåç»
ååé10000ã100000ã®èã䜿çšããéå€æ¿Ÿéæ¹
æ³ãã²ã«æ¿Ÿéãé å¿åé¢ã掻æ§çåŠçãæ¿çž®çã
åç¬ã§ãããã¯çµã¿åãããŠé©çšããŠåç»é€å»ã
ããããã«ãé žãé µçŽ çã«ããäœååååŠçãé©
çšããããšãã§ããã äžèšãããããã¢ãŒãŒãŸãã¯é žåŠçã«ããå€ç³
äœRDPç©è³ªã«å€Ÿéããèçœè³ªã®å€§éšåã¯é€å»ã
ããããäžéšå€ç³äœRDPç©è³ªã«ååŠçã«çµåã
ãèçœè³ªã¯å®å šã«ã¯é€å»ãããªãå Žåããããã
ã®å Žåã¯ããã«ã»ããŒã°æ³çã®é€èçœæ³ã䜿çšã
ãããšã«ããå®å šé€èçœãè¡ãªãããšãã§ããã äžèšããææ©æº¶åªã«ããæŽæµãé µçŽ åŠçãé€è
çœãã€ãªã³äº€æåŠçãéå€æ¿Ÿéãã²ã«æ¿Ÿéãé å¿
åé¢ã掻æ§çåŠçããã³æ¿çž®çã®ç²Ÿè£œåŠçã¯ãã
ã®æäœé åºãçµã¿åããæ¹ãªã©ã«å¶éã¯ãªãå¿ èŠ
ã«å¿ããŠåç¬ãããã¯ïŒçš®ä»¥äžãçµã¿åãããŠé©
çšããã°ããã åèšã®æäœã«ãã粟補ãããé«ååã®å€ç³äœ
RDPç©è³ªãå«ã氎溶液ãåçµä¹Ÿç¥ãåŽé§ä¹Ÿç¥ã
極æ§ææ©æº¶åªã«ããæ²ã§ããªã©ãè¡ãªã€ãŠçœè²ã®
ç²æ«ç¶ãšããããšãã§ããã ãã®ããã«ããŠåŸãããå€ç³äœRDPç©è³ªã¯æ¬¡
ã®ãããªçååŠçæ§è³ªãæããŠãããéæèãé
éãããææ©é žãŸãã¯ææ©æº¶åªãããšãã°ã¢ã«ã³
ãŒã«ïŒã¡ã¿ããŒã«ããšã¿ããŒã«ããããããŒã«ã
ãã¿ããŒã«çïŒãã¢ã»ãã³ããããµã³ããã³ãŒã³ã
é ¢é žãšãã«ããžã¡ãã«ã¹ã«ããã·ãããªã°ãã€
ã³ãåå¡©åççŽ ãã¯ãããã«ã ããšãŒãã«çã«äž
溶ã§ããããæ°Žã«ã¯å¯æº¶ã§ããããŸããæ¬ç©è³ªã®
ïŒïŒ 氎溶液ã®PHã¯äžæ§ã§ãããæ¬ç©è³ªã¯äžå®ã®è
ç¹ã瀺ããã220âã§è€å€ãã280âã§é»å€ããŠç
åãèµ·ããåŸèšè£œé äŸïŒã§ä»£è¡šãããæ¬ç©è³ªã®å
çŽ åæã®çµæã¯ãççŽ 38.10ïŒ ãæ°ŽçŽ 6.27ïŒ ãé ž
çŽ 50.73ïŒ ãç¡æ©è³ª4.90ïŒ ã§ãã€ãããŸããæ¬çº
æã®æ¹æ³ã«ããåŸãç©è³ªã®ïŒïŒ 氎溶液ã®åè²åå¿
ã¯ã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«é žåå¿ãããª
ãããã¢ã³ç¡«é žåå¿ãã·ã¹ãã€ã³ç¡«é žåå¿ãã¯ã
ã¢ãããŒãç¡«é žåå¿ãããšããŒã«ç¡«é žåå¿ãã«ã«
ããŸãŒã«ç¡«é žåå¿ãããããéœæ§ã§ãããããŠãŒ
ã¬ããåå¿ããã³ãããªã³åå¿ãããŒãªãŒããªãŒ
ãªã³åå¿ããšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ã¯
é°æ§ã§ããã ãŸããåŸèšè£œé äŸïŒã§ä»£è¡šãããæ¬ç©è³ªã®æ¯æ
å 床ã¯ãαã20 DïŒïŒ142ããïŒ145ãïŒH2OïŒã§ãã€ãã
次ã«ãç¡æ©è³ªã«ã€ããŠã¯äž»ãšããŠSiïŒïŒ°ïŒïŒ«ïŒ
NaïŒCaïŒMgãªã©ãå«ãŸããŠããããšã確èªã
ãããããå çŽ ã¯RDPç©è³ªãã»ãã¢ããŒãºCLâ
6BïŒãã¢ã«ãã·ã¢è£œïŒã«ãŠã²ã«æ¿Ÿéãããšããç³
æåãšæåãå ±ã«ãããã€ãããªãŠãŒã ã«çŸãã
ãããšããèããŠãç¡æ©è³ªãšããŠåç¬ã§RDPç©
質ã«å€ŸéããŠããã®ã§ã¯ãªããRDPç©è³ªã®éªšæ Œ
æåã«ååŠçã«çµåããŠååšããŠãããã®ãšæšå®
ãããã ããã«ãæ¬ç©è³ªã®ïŒïŒ 氎溶液ã«1Nç¡«é žã«ãªã
ããã«ç¡«é žãå ãã100âã§ïŒæéå æ°Žå解ãè¡
ãªãã次ãã§çé žããªãŠã ãå ããŠäžåããåŸã®
äžæž 液ã¯ã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«é žå
å¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ãã€ã³ç¡«é žå
å¿ãã¯ãã¢ãããŒãç¡«é žåå¿çã¯ããããéœæ§ã§
ãã€ããããŠãŒã¬ããåå¿ããã³ãããªã³åå¿ã
ããŒãªãŒããªãŒãªã³åå¿çã¯é°æ§ã§ãã€ããäž
æ¹ãåœè©²å解液ã®èå±€ã¯ãããã°ã©ãã€ãŒãè¡ãª
ããšãå¿ ãã°ã«ã³ãŒã¹ã®ã¿ãæ€åºããã ãŸããRDPç©è³ªã®ç¡«é žããã³ã®é žã«ããå®å š
å æ°Žå解ç©ã®èå±€ã¯ãããã°ã©ãã€ãŒãäžèšã®ïŒ
çš®é¡ã®å±é溶åªã§å±éãããšãããããããã°ã«
ã³ãŒã¹ä»¥å€ã®ç³ã¹ããããæ€åºããªãã®ã§ãæ¬ç©
質ã¯ã°ã«ã³ãŒã¹ã®ã¿ãæ§æç³ãšããå€ç³ã§ããã
ãšãå€æããã (1)é ¢é žãšãã«ïŒã¡ã¿ããŒã«ïŒé ¢é žïŒæ°Ž 65ïŒ15ïŒ10ïŒ10 (2)é ¢é žãšãã«ïŒã€ãœãããããŒã«ïŒæ°Ž 65ïŒ23ïŒ12 (3)ã€ãœãããããŒã«ïŒããªãžã³ïŒæ°ŽïŒé ¢é ž ïŒïŒïŒïŒïŒïŒïŒ (4)ïœâãã¿ããŒã«ïŒããªãžã³ïŒæ°Ž ïŒïŒïŒïŒïŒ ããã«ãæ¬ç©è³ªã¯ç¬¬ïŒå³ã«ç€ºã玫å€éšåžåã¹ã
ã¯ãã«ã第ïŒå³ã«ç€ºãèµ€å€éšåžåã¹ãã¯ãã«ãã
ã³ç¬¬ïŒå³ã«ç€ºã13CâNMRã¹ãã¯ãã«ã瀺ãã
ãããã®ã¹ãã¯ãã«ã®è§£æãšæ¯æå 床ã®çµæã
ããαçµåã®ååšãæšå®ãããããŸããæ¬ç©è³ªã
éæèãééãããã»ãã¢ããŒãºCLâ6BïŒãã¢
ã«ãã·ã¢è£œïŒã®ã²ã«æ¿Ÿéã§ãã€ãããªãŠãŒã ã«çŸ
ãããç¹ãããæ¬ç©è³ªã¯ã°ã«ã³ãŒã¹ãå¯äžã®ç³æ§
ææåãšããå€ç³äœã§ãããšèªããããã ãŸããRDPç©è³ªã¯éãšãŠçŽ é žé žåå®éšã«ãã
ã°ã«ã³ãŒã¹æ®åºïŒååœãçŽ1.7ã¢ã«ã®éãšãŠçŽ é ž
ãæ¶è²»ããçŽ0.85ã¢ã«ã®ã®é žãçããããšãã¹ã
ã¹å解ãè¡ãªã€ã溶液ãããŒããŒã¯ãããã°ã©ã
ã€ãŒã§åæãããšå€éã®ã°ãªã»ãªã³ãæ€åºããã
ãšãã¡ãã«åç³ã®ããŒããŒã¯ãããã°ã©ã ããå°
éã®ããã©ã¡ãã«ã°ã«ã³ãŒã¹ããžã¡ãã«ã°ã«ã³ãŒ
ã¹ã®ä»ã«å€éã®ïŒïŒïŒïŒïŒâããªã¡ãã«ã°ã«ã³ãŒ
ã¹ãæ€åºããããšçã®å®éšçµæããèããŠÎ±â
ïŒïŒïŒã°ã«ã³ã·ãçµåãäž»äœãšããå€ç³ã§ããã
ãšãæšå®ããããããã«ãRDPç©è³ªã13Câ
NMRã§è§£æããã¹ãã¯ãã«ããæ¬ç©è³ªã¯Î±â
ïŒïŒïŒã°ã«ã³ã·ãçµåãäž»äœã§ããããå°éã®Î±
âïŒïŒïŒçµåããå«æãããã®ååšæ¯ã¯çŽ10察ïŒ
ã§ããããšãå€æããã æ¬çºæã«ããåŸãããå€ç³äœRDPç©è³ªã¯ãæ
è «ç掻æ§ãå ç«èª¿ç¯æŽ»æ§ãææé²åŸ¡æŽ»æ§çã®çš®ã
ã®çç掻æ§ãæããŠããããšãå€æããã以äžã«
ããããã®çç掻æ§ã«ã€ããŠãã®æ€å®æ³ããã³åŸ
è¿°ãã補é äŸïŒã§åŸãããå€ç³äœRDPç©è³ªãæ
äžããå®éšã§ã®æ€å®çµæã«ã€ããŠè©³è¿°ããã (1) æè «ç掻æ§ã«ã€ã㊠(ã€) åç³»è «çã¡ã¹âã«å¯ŸããRDPç©è³ªã®è ¹è
æäžã®å¹æ ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®BALBïŒïŒ£âCRJ
ããŠã¹ã«ïŒé±éãåç³»ã®ããŠã¹ã®è ¹è å ã§ç¶ä»£ã
ãç现èã¡ã¹âãããŠã¹ïŒå¹åœãïŒÃ105åã
è ¹è å ã«ç§»æ€ããå¯Ÿç §çŸ€20å¹ïŒïŒçŸ€ïŒãè©ŠéšçŸ€å
10å¹ïŒïŒçŸ€ïŒã®èšïŒçŸ€ã«åãããç现èã移æ€ã
ãç¿æ¥ããé£ç¶ïŒæ¥éãè©ŠéšçŸ€ã«ã¯ççé£å¡©æ°Žã«
溶解ããRDPç©è³ªãããŠã¹ïŒå¹ã®äœéïŒKgåœã
å10ïŒ30ïŒ100mgã0.1mlãã€è ¹è å ã«æäžãã察
ç §çŸ€ã«ã¯åæ§ã«ããŠççé£å¡©æ°Žã®ã¿ãæäžããã
以åŸãçåæ¥æ°ã芳å¯ãã延åœå¹æã次åŒã«ãã
ç®åºããã 延åœçïŒïŒ ïŒïŒè©ŠéšçŸ€ã®å¹³åçåæ¥æ°ïŒå¯Ÿç §çŸ€ã®å¹³å
çåæ¥æ°Ã100 (ã) åç³»è «çã¡ã¹âã«å¯ŸããRDPç©è³ªã®çµå£
æäžã®å¹æ ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®BALBïŒïŒ£âCRJ
ããŠã¹ã«ïŒé±éãåç³»ã®ããŠã¹ã®è ¹è å ã§ç¶ä»£ã
ãç现èã¡ã¹âãããŠã¹ïŒå¹åœãïŒÃ104åã
å³è äžç®äžã«ç§»æ€ããå¯Ÿç §çŸ€20å¹ïŒïŒçŸ€ïŒãè©Šéš
矀å10å¹ïŒïŒçŸ€ïŒã®èšïŒçŸ€ã«åãããç现èã移
æ€ããç¿æ¥ããé£ç¶10æ¥éãè©ŠéšçŸ€ã«ã¯ççé£å¡©
æ°Žã«æº¶è§£ããRDPç©è³ªãããŠã¹ïŒå¹ã®äœéïŒKg
åœãå10ïŒ30ïŒ100mgã0.2mlãã€çµå£ãŸã³ããçš
ããŠèã«æäžããå¯Ÿç §çŸ€ã«ã¯åæ§ã«ããŠççé£å¡©
æ°Žã®ã¿ãæäžãããç现èã移æ€ããŠãã35æ¥åŸ
ã«åããŠã¹ãå± æ®ºããå¢æ®ããè «çãåãåºãé
éã枬å®ããããªããé»æ¢çã¯æ¬¡åŒã«ããç®åºã
ãã é»æ¢çïŒïŒ ïŒïŒïŒïŒâè©ŠéšçŸ€ã®å¹³åè «çééïŒå¯Ÿç §çŸ€
ã®å¹³åè «çééïŒ Ã100 äžèš(ã€)ïŒ(ã)ã®æ¹æ³ã«ããæ€å®ããRDPç©è³ªã®
æè «çå¹æã¯äžè¡šã®éãã§ãã€ãã
èçœæäœããããšã«ããåŸãããäžèšã®æ§è³ªãæ
ããæ°èŠå€ç³äœRDPç©è³ªããã®è£œé æ³ããã³ã
ããæå¹æåãšããæè «çå€ãå ç«èª¿ç¯å€ããã³
ææçäºé²æ²»çå€ã«é¢ãã詳ããã¯åªããæè «ç
掻æ§ãå ç«èª¿ç¯æŽ»æ§ããã³ææé²åŸ¡æŽ»æ§ãæãã
æ°èŠå€ç³äœRDPç©è³ªãšãç±³ç³ ãåæãšããŠåœè©²
ç©è³ªãæœåºã粟補ããåœè©²ç©è³ªã®è£œé æ³ã«é¢ã
ãããªããRDPãšã¯Rice bran De Proteinã®é
æåããšã€ãç¥èªã§ãããç±³ç³ ããè±èçœããã
ã®ãšããæå³ã§ããã (1) ååéïŒéæèãééãããååéïŒäžä»¥äž
ãšæšå®ãããã (2) 溶解æ§ïŒã¢ã«ã³ãŒã«ãã¢ã»ãã³ããããµã³ã
ãã³ãŒã³ãé ¢é žãšãã«ããžã¡ãã«ã¹ã«ãã
ã·ãããªã°ãã€ã³ãåå¡©åççŽ ãã¯ããã
ã«ã ããã³ãšãŒãã«ã«äžæº¶ã§ãããæ°Žã«ã¯
å¯æº¶ã§ããã (3) 氎溶液ã®PHïŒïŒïŒ 氎溶液ã¯äžæ§ã瀺ãã (4) èçœè³ªãç¡æ©è³ªïŒèçœè³ªãå«æãããïŒïŒ 以
äžã®ç¡æ©è³ªãå«æããã (5) åè²åå¿ïŒã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«
é žåå¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ã
ã€ã³ç¡«é žåå¿ãã¯ãã¢ãããŒãç¡«é žåå¿ã
ããšããŒã«ç¡«é žåå¿ãã«ã«ããŸãŒã«ç¡«é žå
å¿ãéœæ§ã§ãããããŠãŒã¬ããåå¿ããã³
ãããªã³åå¿ãããŒãªãŒããªãŒãªã³åå¿ã
ãšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ãé°æ§
ã§ããã (6) 玫å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã玫å€éš
åžåã¹ãã¯ãã«ã瀺ãã (7) èµ€å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºãèµ€å€éš
åžåã¹ãã¯ãã«ã瀺ãã (8) 13CâNMRã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã13Câ
NMRã¹ãã¯ãã«ã瀺ãã (9) æ§æç³ïŒã°ã«ã³ãŒã¹ã®ã¿ã§æ§æãããã (10) æ§é ïŒÎ±âïŒïŒïŒã°ã«ã³ã·ãçµåãäž»éªšæ Œãš
ããçŽ10åã®Î±âïŒïŒïŒçµåã§çµã°ããã°
ã«ã³ãŒã¹æ®åºã«ïŒåã®å²åã§Î±âïŒïŒïŒçµ
åãæããã (11) èç¹ïŒäžå®ã®èç¹ã瀺ããã220âã§è€å€ãã
280âã§é»å€ããŠçåãèµ·ããã ãã€ãŠãæ®»ç²ãè±ç²çãããã¯ãããã®è¡šå±€éš
ãåæãšããŠçç掻æ§ãæããç©è³ªã補é ããæ¹
æ³ãææ¡ãããŠãããããããã®æ¹æ³ã¯ååŸéã
éåžžã«å°ãªããæ¯æ§ãã¿ãããçã®åé¡ç¹ãã
ãã æ¬çºæã¯ãããã®åé¡ç¹ã解æ¶ãã¹ãéæç 究
ãç¶ããçµæãªããããã®ã§ããã æ¬çºæã®å€ç³äœRDPç©è³ªã¯ç±³ç³ ãç±æ°ŽåŠçã
ãŠåŸãããæœåºæ¶²ã«æ¥µæ§ææ©æº¶åªãå ããããã
ããã¯å¡©æå€ãå ããçããæ²ã§ããååããã®
ã¡æ°Žã«æº¶è§£ããé€èçœæäœãå¿ èŠã«å¿ããŠç²Ÿè£œåŠ
çãç²æ«åŠçãè¡ãªãããšã«ãã€ãŠè£œé ããããš
ãã§ããã æ¬çºæã«çšããããåæç±³ç³ ã¯éåžžã®ç²Ÿç±³ã«ã
ããŠçºçããç±³ç³ ã§ãããåœè©²ç±³ç³ ã®çºçæºã§ã
ãçç±³ã®åçš®ãç£å°ããã³ç²Ÿçœæ©ççãåããªã
ãããã®åæç±³ç³ ããã®å€ç³äœRDPç©è³ªã®æœåºã
粟補ã«å ç«ã€ãŠåœè©²åæäžã«æ··åšããç ç±³çã¯å¯
åçã«é€å»ããæŽæµããããšãæãŸããããŸãã
ç±³ç³ æ²¹ãæœåºããåŸã®æ®æž£ã§ããè±èç³ çã®ãã
ã«ãä»ã®ç®çã«äœ¿çšãããåŸã®ç±³ç³ ã§ãã€ãŠãæ¬
çºæã«äœ¿çšããããšãã§ããã ç±³ç³ ã®æœåºæ¶²ã¯ãç±³ç³ ãç±æ°ŽåŠçããããšã«ã
ãåŸããããç±æ°ŽåŠçã¯çŽ°ããç²ç ããç±³ç³ ã«å¯Ÿ
ãçŽïŒã10åéïŒééïŒã®èžçæ°ŽãŸãã¯ç²Ÿè£œæ°Žã
å ããã¹ãã³ã¬ã¹ã¿ã³ã¯ãããŒããŒåŒãã¿ã³ã¯ã
ã¬ã©ã¹è£œã¿ã³ã¯ãæµéåŒã®ç®¡ç¶æœåºè£ 眮çã®å®¹åš
ãããã¯ãããã®èå§æ§å®¹åšãçšããŠæ¹æãããŸ
ãã¯æ¹æããªãã§ïŒã15KgïŒcm2ã奜ãŸããã¯ïŒã
5.0KgïŒcm2ã®å§åã70ã200âã奜ãŸããã¯100ã
150âã®æž©åºŠã§10åä¹è³24æéã奜ãŸããã¯0.5ã
ïŒæéæ¹æããªããæœåºãè¡ãªããå®çšäžã¯ïŒã
3.0KgïŒcm2ã®å§åã100ã140âã®æž©åºŠã§ïŒãïŒæ
éã®ç±æ°ŽåŠçãé©åœã§ããã ãªãã該åŠçã«å ç«ã€ãŠãç±³ç³ ã¯æ°ŽæŽåŸãé ¢é ž
ãšãã«ãåå¡©åçé žãã¯ãããã«ã ããšãŒãã«ã
ïœâãããµã³ããã³ãŒã³ãç³æ²¹ãšãŒãã«ãã¢ã»ã
ã³çã®ææ©æº¶åªã«ãã€ãŠè溶æ§åºåã®é€å»ãè¡ãª
ãããšãæãŸããã åèšç±æ°ŽåŠçã«ããåŸãããæœåºæ¶²ã¯æ¿Ÿéãã
ãã¯é å¿åé¢çã®æäœã«ãã€ãŠåºåœ¢åãšåé¢ãã
å¿ èŠã«å¿ããŠé©åœãªæ段ãããšãã°æžå§æ¿çž®ãé
å€æ¿Ÿéçã®æ段ãåç¬ãããã¯çµåããŠè¡ãªãé©
åœéãŸã§æ¿çž®ããã ãã®æœåºæ¶²ã«å¯ŸããŠæ°Žã«å¯æº¶ãªæ¥µæ§ææ©æº¶åªã
ããã¯å¡©æå€ãå ããçããæ²ã§ããååããã
ãšã«ãã€ãŠå€ç³äœRDPç©è³ªãå«ãç»åãåŸãã
ãšãã§ãããããã§çšãã極æ§ææ©æº¶åªãšããŠ
ã¯ãããšãã°ã¡ã¿ããŒã«ããšã¿ããŒã«ããããã
ãŒã«ãã¢ã»ãã³çãããã極æ§ææ©æº¶åªã®äœ¿çšé
ã¯æœåºæ¶²äžã«ãããç®çç©è³ªã®å«æéçãèæ ®ã
ãŠæ±ºå®ãããããšã¿ããŒã«ãçšããå ŽåãäŸã«ã
ããšããšã¿ããŒã«æ¿åºŠã30ã50ïŒ ïŒïŒ¶ïŒïŒ¶ïŒã«ãª
ãããã«æ·»å ããã°ããããªããçããæ²ã§ãã¯
åèšãšã¿ããŒã«çã®ææ©æº¶åªã§æŽæµããããšã奜
ãŸãããäžæ¹ãå¡©æå€ãšããŠã¯å¡©åãããªãŠã ã
ç¡«é žã¢ã³ã¢ããŠã ãå¡©åã«ãªãŠã çããããéåžž
ã¯ç±æ°Žæœåºæ¶²ã«å¯Ÿãå¡©æå€ã飜å床0.5ãïŒã«ãª
ããŸã§å ããŠæ²ã§ããçæããããã æ¬çºæã§ã¯ã極æ§ææ©æº¶åªãããã¯å¡©æå€ãç±
æ°Žæœåºæ¶²ã«å ããåãããã¯äžèšã®åŠããããç©
質ãæ·»å ããŠæ²ã§ããçæãããã次ãã§æ°Žã«æº¶
解ããåŸã«ãé€èçœåŠçãå¿ èŠã«å¿ããŠç²Ÿè£œåŠç
ããã³ç²æ«ååŠçãåç¬ã§ãããã¯é©å®çµåããŠ
è¡ãªãããšãã§ãããé€èçœåŠçã粟補åŠçãšã
ãŠã¯çš®ã ã®æäœãé©çšããããšãã§ãããããšã
ã°å€ç³äœRDPç©è³ªãå«æãã溶液ã«æŸ±ç²åè§£é µ
çŽ ããã³ïŒãŸãã¯èçœåè§£é µçŽ ãäœçšãããŠæ··åš
ããŠãã柱ç²ãèçœè³ªãªã©ã®äžçŽç©ãäœåååã
ããäœåååããããã®ã¯åŸã®ç²Ÿè£œå·¥çšã§é€å»ã
ãããé µçŽ ã¯ä»»æã®ãã®ã䜿çšããããšãã§ãã
ããšãã°Î±âã¢ãã©ãŒãŒãã€ãœã¢ãã©ãŒãŒããã«
ã©ããŒãŒãªã©ã®çš®ã ã®ã¢ãã©ãŒãŒãããã€ã³ãã
ãã·ã³ãããªãã·ã³ãããããŒãŒãªã©ã®çš®ã ã®ã
ããã¢ãŒãŒãå¿ èŠãªãã°ä»ã®é µçŽ ãä»»æã«äœ¿çšã§
ãããé µçŽ åŠçã¯ã奜ãŸããã¯é µçŽ ãåºè³ªã®1/10
ïŒïŒã1/5000ã®å²åã§æ·»å ãã0.5ã24æéã奜ãŸ
ããã¯ïŒã15æéè¡ãªãã ä»ã®é€èçœåŠçããã³ç²Ÿè£œåŠçãšããŠãåèšå€
ç³äœRDPç©è³ªå«æ氎溶液ã«æ°·é ¢é žãç¡«é žãå¡©é žã
ã¿ã³ãã³é žãããªã¯ãã«é ¢é žãªã©ã®ç¡æ©é žããã
ã¯ææ©é žã0.1ã10ïŒ ã奜ãŸããã¯ïŒãïŒïŒ çšåºŠ
å ããæ²ã§ããçæãããªãã°æ¿Ÿéãé å¿åé¢ç
ã®æäœã«ããé€ããç¶ããŠæ®åããé žãç¡æ©ã€ãª
ã³ããã³äœåååºåãæµæ°ŽãŸãã¯èžçæ°Žäžã§ã»ã
ãã¢ã³èãã³ããžãªã³èçã®éæèã«ãŠïŒãïŒæ¥
éæããæ¹æ³ãããŠãšãã¯ã¹ãã¢ã³ããŒã©ã€ãã
ããŠãªã©ã€ãããã€ã€ã€ãªã³çã®ã«ããªã³ãã¢ã
ãªã³äº€ææš¹èãçšããã€ãªã³äº€æåŠçæ¹æ³ãåç»
ååé10000ã100000ã®èã䜿çšããéå€æ¿Ÿéæ¹
æ³ãã²ã«æ¿Ÿéãé å¿åé¢ã掻æ§çåŠçãæ¿çž®çã
åç¬ã§ãããã¯çµã¿åãããŠé©çšããŠåç»é€å»ã
ããããã«ãé žãé µçŽ çã«ããäœååååŠçãé©
çšããããšãã§ããã äžèšãããããã¢ãŒãŒãŸãã¯é žåŠçã«ããå€ç³
äœRDPç©è³ªã«å€Ÿéããèçœè³ªã®å€§éšåã¯é€å»ã
ããããäžéšå€ç³äœRDPç©è³ªã«ååŠçã«çµåã
ãèçœè³ªã¯å®å šã«ã¯é€å»ãããªãå Žåããããã
ã®å Žåã¯ããã«ã»ããŒã°æ³çã®é€èçœæ³ã䜿çšã
ãããšã«ããå®å šé€èçœãè¡ãªãããšãã§ããã äžèšããææ©æº¶åªã«ããæŽæµãé µçŽ åŠçãé€è
çœãã€ãªã³äº€æåŠçãéå€æ¿Ÿéãã²ã«æ¿Ÿéãé å¿
åé¢ã掻æ§çåŠçããã³æ¿çž®çã®ç²Ÿè£œåŠçã¯ãã
ã®æäœé åºãçµã¿åããæ¹ãªã©ã«å¶éã¯ãªãå¿ èŠ
ã«å¿ããŠåç¬ãããã¯ïŒçš®ä»¥äžãçµã¿åãããŠé©
çšããã°ããã åèšã®æäœã«ãã粟補ãããé«ååã®å€ç³äœ
RDPç©è³ªãå«ã氎溶液ãåçµä¹Ÿç¥ãåŽé§ä¹Ÿç¥ã
極æ§ææ©æº¶åªã«ããæ²ã§ããªã©ãè¡ãªã€ãŠçœè²ã®
ç²æ«ç¶ãšããããšãã§ããã ãã®ããã«ããŠåŸãããå€ç³äœRDPç©è³ªã¯æ¬¡
ã®ãããªçååŠçæ§è³ªãæããŠãããéæèãé
éãããææ©é žãŸãã¯ææ©æº¶åªãããšãã°ã¢ã«ã³
ãŒã«ïŒã¡ã¿ããŒã«ããšã¿ããŒã«ããããããŒã«ã
ãã¿ããŒã«çïŒãã¢ã»ãã³ããããµã³ããã³ãŒã³ã
é ¢é žãšãã«ããžã¡ãã«ã¹ã«ããã·ãããªã°ãã€
ã³ãåå¡©åççŽ ãã¯ãããã«ã ããšãŒãã«çã«äž
溶ã§ããããæ°Žã«ã¯å¯æº¶ã§ããããŸããæ¬ç©è³ªã®
ïŒïŒ 氎溶液ã®PHã¯äžæ§ã§ãããæ¬ç©è³ªã¯äžå®ã®è
ç¹ã瀺ããã220âã§è€å€ãã280âã§é»å€ããŠç
åãèµ·ããåŸèšè£œé äŸïŒã§ä»£è¡šãããæ¬ç©è³ªã®å
çŽ åæã®çµæã¯ãççŽ 38.10ïŒ ãæ°ŽçŽ 6.27ïŒ ãé ž
çŽ 50.73ïŒ ãç¡æ©è³ª4.90ïŒ ã§ãã€ãããŸããæ¬çº
æã®æ¹æ³ã«ããåŸãç©è³ªã®ïŒïŒ 氎溶液ã®åè²åå¿
ã¯ã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«é žåå¿ãããª
ãããã¢ã³ç¡«é žåå¿ãã·ã¹ãã€ã³ç¡«é žåå¿ãã¯ã
ã¢ãããŒãç¡«é žåå¿ãããšããŒã«ç¡«é žåå¿ãã«ã«
ããŸãŒã«ç¡«é žåå¿ãããããéœæ§ã§ãããããŠãŒ
ã¬ããåå¿ããã³ãããªã³åå¿ãããŒãªãŒããªãŒ
ãªã³åå¿ããšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ã¯
é°æ§ã§ããã ãŸããåŸèšè£œé äŸïŒã§ä»£è¡šãããæ¬ç©è³ªã®æ¯æ
å 床ã¯ãαã20 DïŒïŒ142ããïŒ145ãïŒH2OïŒã§ãã€ãã
次ã«ãç¡æ©è³ªã«ã€ããŠã¯äž»ãšããŠSiïŒïŒ°ïŒïŒ«ïŒ
NaïŒCaïŒMgãªã©ãå«ãŸããŠããããšã確èªã
ãããããå çŽ ã¯RDPç©è³ªãã»ãã¢ããŒãºCLâ
6BïŒãã¢ã«ãã·ã¢è£œïŒã«ãŠã²ã«æ¿Ÿéãããšããç³
æåãšæåãå ±ã«ãããã€ãããªãŠãŒã ã«çŸãã
ãããšããèããŠãç¡æ©è³ªãšããŠåç¬ã§RDPç©
質ã«å€ŸéããŠããã®ã§ã¯ãªããRDPç©è³ªã®éªšæ Œ
æåã«ååŠçã«çµåããŠååšããŠãããã®ãšæšå®
ãããã ããã«ãæ¬ç©è³ªã®ïŒïŒ 氎溶液ã«1Nç¡«é žã«ãªã
ããã«ç¡«é žãå ãã100âã§ïŒæéå æ°Žå解ãè¡
ãªãã次ãã§çé žããªãŠã ãå ããŠäžåããåŸã®
äžæž 液ã¯ã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«é žå
å¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ãã€ã³ç¡«é žå
å¿ãã¯ãã¢ãããŒãç¡«é žåå¿çã¯ããããéœæ§ã§
ãã€ããããŠãŒã¬ããåå¿ããã³ãããªã³åå¿ã
ããŒãªãŒããªãŒãªã³åå¿çã¯é°æ§ã§ãã€ããäž
æ¹ãåœè©²å解液ã®èå±€ã¯ãããã°ã©ãã€ãŒãè¡ãª
ããšãå¿ ãã°ã«ã³ãŒã¹ã®ã¿ãæ€åºããã ãŸããRDPç©è³ªã®ç¡«é žããã³ã®é žã«ããå®å š
å æ°Žå解ç©ã®èå±€ã¯ãããã°ã©ãã€ãŒãäžèšã®ïŒ
çš®é¡ã®å±é溶åªã§å±éãããšãããããããã°ã«
ã³ãŒã¹ä»¥å€ã®ç³ã¹ããããæ€åºããªãã®ã§ãæ¬ç©
質ã¯ã°ã«ã³ãŒã¹ã®ã¿ãæ§æç³ãšããå€ç³ã§ããã
ãšãå€æããã (1)é ¢é žãšãã«ïŒã¡ã¿ããŒã«ïŒé ¢é žïŒæ°Ž 65ïŒ15ïŒ10ïŒ10 (2)é ¢é žãšãã«ïŒã€ãœãããããŒã«ïŒæ°Ž 65ïŒ23ïŒ12 (3)ã€ãœãããããŒã«ïŒããªãžã³ïŒæ°ŽïŒé ¢é ž ïŒïŒïŒïŒïŒïŒïŒ (4)ïœâãã¿ããŒã«ïŒããªãžã³ïŒæ°Ž ïŒïŒïŒïŒïŒ ããã«ãæ¬ç©è³ªã¯ç¬¬ïŒå³ã«ç€ºã玫å€éšåžåã¹ã
ã¯ãã«ã第ïŒå³ã«ç€ºãèµ€å€éšåžåã¹ãã¯ãã«ãã
ã³ç¬¬ïŒå³ã«ç€ºã13CâNMRã¹ãã¯ãã«ã瀺ãã
ãããã®ã¹ãã¯ãã«ã®è§£æãšæ¯æå 床ã®çµæã
ããαçµåã®ååšãæšå®ãããããŸããæ¬ç©è³ªã
éæèãééãããã»ãã¢ããŒãºCLâ6BïŒãã¢
ã«ãã·ã¢è£œïŒã®ã²ã«æ¿Ÿéã§ãã€ãããªãŠãŒã ã«çŸ
ãããç¹ãããæ¬ç©è³ªã¯ã°ã«ã³ãŒã¹ãå¯äžã®ç³æ§
ææåãšããå€ç³äœã§ãããšèªããããã ãŸããRDPç©è³ªã¯éãšãŠçŽ é žé žåå®éšã«ãã
ã°ã«ã³ãŒã¹æ®åºïŒååœãçŽ1.7ã¢ã«ã®éãšãŠçŽ é ž
ãæ¶è²»ããçŽ0.85ã¢ã«ã®ã®é žãçããããšãã¹ã
ã¹å解ãè¡ãªã€ã溶液ãããŒããŒã¯ãããã°ã©ã
ã€ãŒã§åæãããšå€éã®ã°ãªã»ãªã³ãæ€åºããã
ãšãã¡ãã«åç³ã®ããŒããŒã¯ãããã°ã©ã ããå°
éã®ããã©ã¡ãã«ã°ã«ã³ãŒã¹ããžã¡ãã«ã°ã«ã³ãŒ
ã¹ã®ä»ã«å€éã®ïŒïŒïŒïŒïŒâããªã¡ãã«ã°ã«ã³ãŒ
ã¹ãæ€åºããããšçã®å®éšçµæããèããŠÎ±â
ïŒïŒïŒã°ã«ã³ã·ãçµåãäž»äœãšããå€ç³ã§ããã
ãšãæšå®ããããããã«ãRDPç©è³ªã13Câ
NMRã§è§£æããã¹ãã¯ãã«ããæ¬ç©è³ªã¯Î±â
ïŒïŒïŒã°ã«ã³ã·ãçµåãäž»äœã§ããããå°éã®Î±
âïŒïŒïŒçµåããå«æãããã®ååšæ¯ã¯çŽ10察ïŒ
ã§ããããšãå€æããã æ¬çºæã«ããåŸãããå€ç³äœRDPç©è³ªã¯ãæ
è «ç掻æ§ãå ç«èª¿ç¯æŽ»æ§ãææé²åŸ¡æŽ»æ§çã®çš®ã
ã®çç掻æ§ãæããŠããããšãå€æããã以äžã«
ããããã®çç掻æ§ã«ã€ããŠãã®æ€å®æ³ããã³åŸ
è¿°ãã補é äŸïŒã§åŸãããå€ç³äœRDPç©è³ªãæ
äžããå®éšã§ã®æ€å®çµæã«ã€ããŠè©³è¿°ããã (1) æè «ç掻æ§ã«ã€ã㊠(ã€) åç³»è «çã¡ã¹âã«å¯ŸããRDPç©è³ªã®è ¹è
æäžã®å¹æ ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®BALBïŒïŒ£âCRJ
ããŠã¹ã«ïŒé±éãåç³»ã®ããŠã¹ã®è ¹è å ã§ç¶ä»£ã
ãç现èã¡ã¹âãããŠã¹ïŒå¹åœãïŒÃ105åã
è ¹è å ã«ç§»æ€ããå¯Ÿç §çŸ€20å¹ïŒïŒçŸ€ïŒãè©ŠéšçŸ€å
10å¹ïŒïŒçŸ€ïŒã®èšïŒçŸ€ã«åãããç现èã移æ€ã
ãç¿æ¥ããé£ç¶ïŒæ¥éãè©ŠéšçŸ€ã«ã¯ççé£å¡©æ°Žã«
溶解ããRDPç©è³ªãããŠã¹ïŒå¹ã®äœéïŒKgåœã
å10ïŒ30ïŒ100mgã0.1mlãã€è ¹è å ã«æäžãã察
ç §çŸ€ã«ã¯åæ§ã«ããŠççé£å¡©æ°Žã®ã¿ãæäžããã
以åŸãçåæ¥æ°ã芳å¯ãã延åœå¹æã次åŒã«ãã
ç®åºããã 延åœçïŒïŒ ïŒïŒè©ŠéšçŸ€ã®å¹³åçåæ¥æ°ïŒå¯Ÿç §çŸ€ã®å¹³å
çåæ¥æ°Ã100 (ã) åç³»è «çã¡ã¹âã«å¯ŸããRDPç©è³ªã®çµå£
æäžã®å¹æ ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®BALBïŒïŒ£âCRJ
ããŠã¹ã«ïŒé±éãåç³»ã®ããŠã¹ã®è ¹è å ã§ç¶ä»£ã
ãç现èã¡ã¹âãããŠã¹ïŒå¹åœãïŒÃ104åã
å³è äžç®äžã«ç§»æ€ããå¯Ÿç §çŸ€20å¹ïŒïŒçŸ€ïŒãè©Šéš
矀å10å¹ïŒïŒçŸ€ïŒã®èšïŒçŸ€ã«åãããç现èã移
æ€ããç¿æ¥ããé£ç¶10æ¥éãè©ŠéšçŸ€ã«ã¯ççé£å¡©
æ°Žã«æº¶è§£ããRDPç©è³ªãããŠã¹ïŒå¹ã®äœéïŒKg
åœãå10ïŒ30ïŒ100mgã0.2mlãã€çµå£ãŸã³ããçš
ããŠèã«æäžããå¯Ÿç §çŸ€ã«ã¯åæ§ã«ããŠççé£å¡©
æ°Žã®ã¿ãæäžãããç现èã移æ€ããŠãã35æ¥åŸ
ã«åããŠã¹ãå± æ®ºããå¢æ®ããè «çãåãåºãé
éã枬å®ããããªããé»æ¢çã¯æ¬¡åŒã«ããç®åºã
ãã é»æ¢çïŒïŒ ïŒïŒïŒïŒâè©ŠéšçŸ€ã®å¹³åè «çééïŒå¯Ÿç §çŸ€
ã®å¹³åè «çééïŒ Ã100 äžèš(ã€)ïŒ(ã)ã®æ¹æ³ã«ããæ€å®ããRDPç©è³ªã®
æè «çå¹æã¯äžè¡šã®éãã§ãã€ãã
ãè¡šã
äžè¡šããæãããªããã«ãè
¹è
æäžãçµå£æäž
ãšãã«ããŠã¹äœéïŒKgåœã30mgä»è¿ãè³é©æäžé
ãšããŠRDPç©è³ªã¯åŒ·ãæè «ç掻æ§ãæããŠãã
ããšãå€æããã ãã®ä»ã«RDPç©è³ªã¯åç³»è «çã«ã€ã¹èºçãã¡
ã©ããŒãâ16ãåçš®è «çã¶ã«ã³ãŒã180ããšãŒ
ã«ãªããè «ççã«å¯Ÿããæäžé10ã100mgïŒKgã®
ç¯å²ã§è ¹è æäžãŸãã¯çµå£æäžã«ããè «çé»æ¢ç
30ã70ïŒ ã®å¹æã確èªãããŠããåŸè¿°ããããã«
æ¯æ§ãå šãèŠãããªãç¹ãšãåãããŠæ¥µããŠæå¹
ãªæè «çå€ãšãªããããšèããããã (2) å ç«èª¿ç¯æŽ»æ§ã«ã€ã㊠(ã€) ã«ãŒãã³ã¯ã¢ã©ã³ã¹ãã¹ãïŒCCTïŒ æ¬æ³ã¯å ç«èª¿ç¯äœçšã®ãã¡ãã¯ããã¢ãŒãžã®é£
现è掻æ§ã®å¢åŒ·å¹æã«ã€ããŠèª¿ã¹ããã®ã§ããã ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®ICRâCRJããŠã¹
ïŒçŸ€ïŒå¹ã«ãççé£å¡©æ°Žã«æº¶è§£ããRDPç©è³ªã
ïŒæ¥éè ¹è æäžãïŒå¯Ÿç §çŸ€ã¯ççé£å¡©æ°Žã®ã¿ãæ
äžïŒãïŒæ¥ç®ã«ã«ãŒãã³æ¶²ïŒããªã«ã³è£œé»ã€ã³ã¯
ïŒåååïŒãã¢ãŠã³ã ã€ã³ãã€ã¢ïŒãççé£å¡©
æ°Žã§ïŒåã«åžéãã液ïŒãããŠã¹å°Ÿéèãã0.25
mlæ³šå ¥ããæ³šå ¥çŽåŸããã³10ååŸã«çŒçª©éèå¢ã
ã0.025mlæ¡è¡ãã3.5mlã®0.01ã¢ã«çé žãããªãŠ
ã 溶液ã«æžæ¿æº¶è§£ããã650nmã®åžå 床
ïŒOD650ïŒã枬å®ããè¡äžã«ãŒãã³æ¿åºŠã®æžå°ç
ã調ã¹ããå¹æã¯æ¬¡åŒã«ç€ºã貧é£ä¿æ°ã§è¡šãã
ãã 貧é£ä¿æ°ïŒïŒ«ïŒïŒlogC1âlogC2ïŒT2âT1 ïŒT1æã«ãããOD650ãC1ïŒT2æã«ããã
OD650ãC2ãšããã ãªããæ çããŠã¹ã«ã€ããŠã¯RDPç©è³ªã®æäž
éå§ããïŒæ¥åã«ã¶ã«ã³ãŒã180现èãïŒÃ107å
å€§è ¿éšçèã«ç§»æ€ãã以äžåæ§ã«è©Šéšãããçµæ
ã¯äžè¡šã®éãã§ãããæ£åžžããŠã¹ãæ çããŠã¹ãš
ãã«RDPç©è³ªã®10ã30mgïŒKgãç¹ã«30mgïŒKgã®
æäžã«ããããŠã¹ã®çŽ°ç¶²å ç®ç³»ã®æ©èœã亢é²ãã
ãã¯ããã¢ãŒãžã®è²§é£èœãå€§å¹ ã«å¢åŒ·ãããŠãã
ããšãå€æããã
ãšãã«ããŠã¹äœéïŒKgåœã30mgä»è¿ãè³é©æäžé
ãšããŠRDPç©è³ªã¯åŒ·ãæè «ç掻æ§ãæããŠãã
ããšãå€æããã ãã®ä»ã«RDPç©è³ªã¯åç³»è «çã«ã€ã¹èºçãã¡
ã©ããŒãâ16ãåçš®è «çã¶ã«ã³ãŒã180ããšãŒ
ã«ãªããè «ççã«å¯Ÿããæäžé10ã100mgïŒKgã®
ç¯å²ã§è ¹è æäžãŸãã¯çµå£æäžã«ããè «çé»æ¢ç
30ã70ïŒ ã®å¹æã確èªãããŠããåŸè¿°ããããã«
æ¯æ§ãå šãèŠãããªãç¹ãšãåãããŠæ¥µããŠæå¹
ãªæè «çå€ãšãªããããšèããããã (2) å ç«èª¿ç¯æŽ»æ§ã«ã€ã㊠(ã€) ã«ãŒãã³ã¯ã¢ã©ã³ã¹ãã¹ãïŒCCTïŒ æ¬æ³ã¯å ç«èª¿ç¯äœçšã®ãã¡ãã¯ããã¢ãŒãžã®é£
现è掻æ§ã®å¢åŒ·å¹æã«ã€ããŠèª¿ã¹ããã®ã§ããã ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®ICRâCRJããŠã¹
ïŒçŸ€ïŒå¹ã«ãççé£å¡©æ°Žã«æº¶è§£ããRDPç©è³ªã
ïŒæ¥éè ¹è æäžãïŒå¯Ÿç §çŸ€ã¯ççé£å¡©æ°Žã®ã¿ãæ
äžïŒãïŒæ¥ç®ã«ã«ãŒãã³æ¶²ïŒããªã«ã³è£œé»ã€ã³ã¯
ïŒåååïŒãã¢ãŠã³ã ã€ã³ãã€ã¢ïŒãççé£å¡©
æ°Žã§ïŒåã«åžéãã液ïŒãããŠã¹å°Ÿéèãã0.25
mlæ³šå ¥ããæ³šå ¥çŽåŸããã³10ååŸã«çŒçª©éèå¢ã
ã0.025mlæ¡è¡ãã3.5mlã®0.01ã¢ã«çé žãããªãŠ
ã 溶液ã«æžæ¿æº¶è§£ããã650nmã®åžå 床
ïŒOD650ïŒã枬å®ããè¡äžã«ãŒãã³æ¿åºŠã®æžå°ç
ã調ã¹ããå¹æã¯æ¬¡åŒã«ç€ºã貧é£ä¿æ°ã§è¡šãã
ãã 貧é£ä¿æ°ïŒïŒ«ïŒïŒlogC1âlogC2ïŒT2âT1 ïŒT1æã«ãããOD650ãC1ïŒT2æã«ããã
OD650ãC2ãšããã ãªããæ çããŠã¹ã«ã€ããŠã¯RDPç©è³ªã®æäž
éå§ããïŒæ¥åã«ã¶ã«ã³ãŒã180现èãïŒÃ107å
å€§è ¿éšçèã«ç§»æ€ãã以äžåæ§ã«è©Šéšãããçµæ
ã¯äžè¡šã®éãã§ãããæ£åžžããŠã¹ãæ çããŠã¹ãš
ãã«RDPç©è³ªã®10ã30mgïŒKgãç¹ã«30mgïŒKgã®
æäžã«ããããŠã¹ã®çŽ°ç¶²å ç®ç³»ã®æ©èœã亢é²ãã
ãã¯ããã¢ãŒãžã®è²§é£èœãå€§å¹ ã«å¢åŒ·ãããŠãã
ããšãå€æããã
ãè¡šã
(ã) ãã©ãŒã¯ããªãŒãã³ã°ã»ã«æ³ïŒPFCïŒ
æ¬æ³ã¯å
ç«èª¿ç¯äœçšã®ãã¡ã宿䞻ã®ïŒ¢çŽ°èã®è³Š
掻ã«ããæäœç£çèœã®å¢åŒ·å¹æã調ã¹ããã®ã§ã
ãã ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®ICRâCRJããŠã¹
ïŒçŸ€ïŒå¹ã«ãççé£å¡©æ°Žã«æº¶è§£ããRDPç©è³ªã
ïŒæ¥éé£ç¶ããŠè ¹è å ã«æäžãïŒå¯Ÿç §çŸ€ã¯ççé£
å¡©æ°Žã®ã¿ãæäžïŒãïŒæ¥ç®ãš11æ¥ç®ã«ããããçŸ
èµ€è¡çïŒÃ108åãå°Ÿéèããæ³šå ¥æäœãããã
ãã®ïŒæ¥åŸã«ã«ãã³ã¬ã ã®æ¹æ³ã§ããŠã¹èŸçŽ°èã®
ãã©ãŒã¯åœ¢æèœã枬å®ããã çµæã¯äžè¡šã®éãã§ãããRDPç©è³ªã¯10ã100
mgïŒKgã®æäžã«ããæäœç£çèœãèããå¢åŒ·ããŠ
ããããšã瀺ãããã
掻ã«ããæäœç£çèœã®å¢åŒ·å¹æã調ã¹ããã®ã§ã
ãã ïŒé±ä»€ã¡ã¹ãå¹³åäœé20ïœã®ICRâCRJããŠã¹
ïŒçŸ€ïŒå¹ã«ãççé£å¡©æ°Žã«æº¶è§£ããRDPç©è³ªã
ïŒæ¥éé£ç¶ããŠè ¹è å ã«æäžãïŒå¯Ÿç §çŸ€ã¯ççé£
å¡©æ°Žã®ã¿ãæäžïŒãïŒæ¥ç®ãš11æ¥ç®ã«ããããçŸ
èµ€è¡çïŒÃ108åãå°Ÿéèããæ³šå ¥æäœãããã
ãã®ïŒæ¥åŸã«ã«ãã³ã¬ã ã®æ¹æ³ã§ããŠã¹èŸçŽ°èã®
ãã©ãŒã¯åœ¢æèœã枬å®ããã çµæã¯äžè¡šã®éãã§ãããRDPç©è³ªã¯10ã100
mgïŒKgã®æäžã«ããæäœç£çèœãèããå¢åŒ·ããŠ
ããããšã瀺ãããã
ãè¡šã
(ã) é
延åç®èåå¿æ§ïŒDHRïŒ
æ¬æ³ã¯å
ç«èª¿ç¯äœçšã®ãã¡å®¿äž»ã®ïŒŽçŽ°èã®è³ŠæŽ»
ã«ãã现èæ§å ç«ã®äœçšã®å¢åŒ·å¹æã調ã¹ããã®
ã§ããã ïŒé±ä»€ã¡ã¹ãå¹³åäœé27ïœã®ICRâCRJããŠã¹
ïŒçŸ€ïŒå¹ã«ãççé£å¡©æ°Žã«æº¶è§£ããRDPç©è³ªã
ïŒæ¥éé£ç¶ããŠçµå£æäžãïŒå¯Ÿç §çŸ€ã¯ççé£å¡©æ°Ž
ã®ã¿ãæäžïŒãæè¬éå§åŸïŒæ¥ç®ã«ããŠã¹ã®åæ¯
è ¹éšã«ïŒïŒ å¡©åãã¯ãªã«ãšã¿ããŒã«æº¶æ¶²ãå¡åžã
ãŠäžæ¬¡æäœãã11æ¥ç®ã«ïŒïŒ ãã¯ãªã«ãªãªãŒãæ²¹
溶液ãããŠã¹äž¡è³ã®è¡šè£ã«å¡åžããŠäºæ¬¡æäœãã
ãã®24æéåŸã«è³åã®å¢å ãã²ãŒãžã§æž¬å®ããå¡
åžåã®è³åãšã®å·®ããè³åã®å¢å éãã¿ããäž
æ¹ãæ çããŠã¹ã«ã€ããŠã¯ã¶ã«ã³ãŒã180è ¹æ°Žå
è «ç现èãïŒÃ105åãæè¬éå§åæ¥ã«ããŠã¹è ¹
è å ã«ç§»æ€ãã以äžåæ§ã«è©Šéšããã çµæã¯äžè¡šã®éãã§ãããRDPç©è³ªã¯è©Šéšã
ã30ã500mgïŒKgã®çµå£æäžã«ãããæ£åžžããŠã¹ã
æ çããŠã¹ãšãã«çŽ°èæ§å ç«èœãèããå¢åŒ·ããŠ
ããããšã瀺ãããã
ã«ãã现èæ§å ç«ã®äœçšã®å¢åŒ·å¹æã調ã¹ããã®
ã§ããã ïŒé±ä»€ã¡ã¹ãå¹³åäœé27ïœã®ICRâCRJããŠã¹
ïŒçŸ€ïŒå¹ã«ãççé£å¡©æ°Žã«æº¶è§£ããRDPç©è³ªã
ïŒæ¥éé£ç¶ããŠçµå£æäžãïŒå¯Ÿç §çŸ€ã¯ççé£å¡©æ°Ž
ã®ã¿ãæäžïŒãæè¬éå§åŸïŒæ¥ç®ã«ããŠã¹ã®åæ¯
è ¹éšã«ïŒïŒ å¡©åãã¯ãªã«ãšã¿ããŒã«æº¶æ¶²ãå¡åžã
ãŠäžæ¬¡æäœãã11æ¥ç®ã«ïŒïŒ ãã¯ãªã«ãªãªãŒãæ²¹
溶液ãããŠã¹äž¡è³ã®è¡šè£ã«å¡åžããŠäºæ¬¡æäœãã
ãã®24æéåŸã«è³åã®å¢å ãã²ãŒãžã§æž¬å®ããå¡
åžåã®è³åãšã®å·®ããè³åã®å¢å éãã¿ããäž
æ¹ãæ çããŠã¹ã«ã€ããŠã¯ã¶ã«ã³ãŒã180è ¹æ°Žå
è «ç现èãïŒÃ105åãæè¬éå§åæ¥ã«ããŠã¹è ¹
è å ã«ç§»æ€ãã以äžåæ§ã«è©Šéšããã çµæã¯äžè¡šã®éãã§ãããRDPç©è³ªã¯è©Šéšã
ã30ã500mgïŒKgã®çµå£æäžã«ãããæ£åžžããŠã¹ã
æ çããŠã¹ãšãã«çŽ°èæ§å ç«èœãèããå¢åŒ·ããŠ
ããããšã瀺ãããã
ãè¡šã
以äžã(ã€)ïŒ(ã)ïŒ(ã)ã®åå
ç«å®éšã«ããRDPç©
質ã¯ã¡ã«ããºã ã®ç°ãªãå ç«äœçšãããããé¡è
ã«äº¢é²ãããŠããããšãããã€ããå ç«èª¿ç¯å€ã¯
äžè¬ã«ã¯çäœã®å ç«æ©èœãäœäžããããç°çš®æå
èªèæ©èœã匱ãå Žåãªã©ã«äœ¿çšããåŸãããšã
ããç¹ã«åŸ®çç©ææçãæªæ§è «çã®æ²»çå€ãæ²»ç
è£åŒ·å€ãŸãã¯äœµçšå€ãäºé²å€ãããã¯è¡åŸå埩ä¿
é²å€ãšããŠã®è¬å€çšéãæåŸ ãããã以äžã®å ç«
賊掻å埩æ©èœã®ä»ã«ããå ç«èª¿ç¯å€ã¯ç°åžžã«äº¢é²
ããçäœå ç«åå¿ãæ£åžžåããããšãã°ãªãŠã
ããè åç ãã¢ã¬ã«ã®ãŒçã®èªå·±å ç«çŸæ£ã«ãé©
çšã§ããå Žåãèããããã (3) ææé²åŸ¡æŽ»æ§ã«ã€ã㊠çäœã®çŽ°èã«ããææçã«å¯Ÿããé²åŸ¡äœçšãšã
ãŠã¯ãäŸµå ¥çŽ°èã«å¯Ÿããæäœç£çã«ãããããã
ãäœæ¶²æ§å ç«äœçšã«ãããã®ãšããã¯ããã¢ãŒãž
ã现èãäŸµå ¥çŽ°èãšæãããããã现èæ§å ç«
ã«ãããã®ãããããšãç¥ãããŠãããäžè¬ã«ã¯
çäœã¯ãããç°çš®çŽ°èã®äŸµå ¥ã«å¯ŸããŠã¯å åãªé²
埡äœçšãæã€ãŠããããæ çç¶æ ãç¹ã«çã®æ«æ
ã«ã¯èããé²åŸ¡äœçšãäœäžããããšãç¥ãããŠã
ããé垞宿䞻ãšå ±çããŠããéç æºèã«ãã€ãŠã
ãé節ãªçµæãææ¥ããããšãç¥ãããŠããã ããã§RDPç©è³ªããããã®çŽ°èã®ææçã«å¯Ÿ
ããŠå®¿äž»ã®é²åŸ¡æŽ»æ§ãå¢åŒ·ãããã©ãããäœæ¶²æ§
å ç«ãé¢äžãããšãããã代衚çææèã§ãããš
ã·ãšãªãã¢ã»ã³ãªïŒEscherichia coliïŒããã³çŽ°
èæ§å ç«ãé¢äžãããšãããããªã¹ããªã¢ã»ã¢ã
ãµã€ãã²ãã¹ïŒListeria monocytogenesïŒææ
ã«å¯ŸããRDPç©è³ªã®å¹æã調ã¹ãã ïŒé±ä»€ã¡ã¹ãå¹³åäœé26ïœã®ICRâCRJããŠã¹
ãïŒçŸ€20å¹ãã€çšããççé£å¡©æ°Žã«æº¶è§£ãã
RDPç©è³ªã10ã100mgïŒKgïŒå¯Ÿç §çŸ€ã¯ççé£å¡©æ°Ž
ã®ã¿ïŒããŠã¹ã®èäžç®äžã«çŽ°èææïŒæ¥åãïŒæ¥
åã«åïŒåæäžããåŸããšã·ãšãªãã¢ã»ã³ãªã®å Ž
åã¯ïŒÃ107åãèäžç®äžã«ããªã¹ããªã¢ã»ã¢ã
ãµã€ãã²ãã¹ã®å Žåã¯ïŒÃ107åãè ¹è å ã«ææ
ãããããããïŒé±é芳å¯ããŠãçæ®ããŠã¹æ°ã
æ¯èŒãããé²åŸ¡å¹æã¯æ¬¡åŒã«ããç®åºããã é²åŸ¡å¹æïŒïŒ ïŒ ïŒè©ŠéšçŸ€ã®çæ®å¹æ°âå¯Ÿç §çŸ€ã®çæ®å¹æ°ïŒïŒçŸ€ã®ããŠ
ã¹æ°Ã100 çµæã¯äžè¡šã«ç€ºãéãã§ãããRDPç©è³ªã®10
ã100mgïŒKgã®äºåæäžã«ããããšã·ãšãªãã¢ã»
ã³ãªææã«å¯ŸããŠã¯éåžžã«åŒ·ãé²åŸ¡äœçšãçãã
ãªã¹ããªã¢ã»ã¢ããµã€ãã²ãã¹ææã«å¯ŸããŠãæ
æãªé²åŸ¡äœçšã®å¢åŒ·å¹æãã¿ãããã åŸè¿°ããããã«ãRDPç©è³ªã¯æ¯æ§ãå šãèŠã
ããªãç¹ãšãåãããŠã極ããŠæå¹ãªææçäºé²
æ²»çå€ãšãªããããšèããããã
質ã¯ã¡ã«ããºã ã®ç°ãªãå ç«äœçšãããããé¡è
ã«äº¢é²ãããŠããããšãããã€ããå ç«èª¿ç¯å€ã¯
äžè¬ã«ã¯çäœã®å ç«æ©èœãäœäžããããç°çš®æå
èªèæ©èœã匱ãå Žåãªã©ã«äœ¿çšããåŸãããšã
ããç¹ã«åŸ®çç©ææçãæªæ§è «çã®æ²»çå€ãæ²»ç
è£åŒ·å€ãŸãã¯äœµçšå€ãäºé²å€ãããã¯è¡åŸå埩ä¿
é²å€ãšããŠã®è¬å€çšéãæåŸ ãããã以äžã®å ç«
賊掻å埩æ©èœã®ä»ã«ããå ç«èª¿ç¯å€ã¯ç°åžžã«äº¢é²
ããçäœå ç«åå¿ãæ£åžžåããããšãã°ãªãŠã
ããè åç ãã¢ã¬ã«ã®ãŒçã®èªå·±å ç«çŸæ£ã«ãé©
çšã§ããå Žåãèããããã (3) ææé²åŸ¡æŽ»æ§ã«ã€ã㊠çäœã®çŽ°èã«ããææçã«å¯Ÿããé²åŸ¡äœçšãšã
ãŠã¯ãäŸµå ¥çŽ°èã«å¯Ÿããæäœç£çã«ãããããã
ãäœæ¶²æ§å ç«äœçšã«ãããã®ãšããã¯ããã¢ãŒãž
ã现èãäŸµå ¥çŽ°èãšæãããããã现èæ§å ç«
ã«ãããã®ãããããšãç¥ãããŠãããäžè¬ã«ã¯
çäœã¯ãããç°çš®çŽ°èã®äŸµå ¥ã«å¯ŸããŠã¯å åãªé²
埡äœçšãæã€ãŠããããæ çç¶æ ãç¹ã«çã®æ«æ
ã«ã¯èããé²åŸ¡äœçšãäœäžããããšãç¥ãããŠã
ããé垞宿䞻ãšå ±çããŠããéç æºèã«ãã€ãŠã
ãé節ãªçµæãææ¥ããããšãç¥ãããŠããã ããã§RDPç©è³ªããããã®çŽ°èã®ææçã«å¯Ÿ
ããŠå®¿äž»ã®é²åŸ¡æŽ»æ§ãå¢åŒ·ãããã©ãããäœæ¶²æ§
å ç«ãé¢äžãããšãããã代衚çææèã§ãããš
ã·ãšãªãã¢ã»ã³ãªïŒEscherichia coliïŒããã³çŽ°
èæ§å ç«ãé¢äžãããšãããããªã¹ããªã¢ã»ã¢ã
ãµã€ãã²ãã¹ïŒListeria monocytogenesïŒææ
ã«å¯ŸããRDPç©è³ªã®å¹æã調ã¹ãã ïŒé±ä»€ã¡ã¹ãå¹³åäœé26ïœã®ICRâCRJããŠã¹
ãïŒçŸ€20å¹ãã€çšããççé£å¡©æ°Žã«æº¶è§£ãã
RDPç©è³ªã10ã100mgïŒKgïŒå¯Ÿç §çŸ€ã¯ççé£å¡©æ°Ž
ã®ã¿ïŒããŠã¹ã®èäžç®äžã«çŽ°èææïŒæ¥åãïŒæ¥
åã«åïŒåæäžããåŸããšã·ãšãªãã¢ã»ã³ãªã®å Ž
åã¯ïŒÃ107åãèäžç®äžã«ããªã¹ããªã¢ã»ã¢ã
ãµã€ãã²ãã¹ã®å Žåã¯ïŒÃ107åãè ¹è å ã«ææ
ãããããããïŒé±é芳å¯ããŠãçæ®ããŠã¹æ°ã
æ¯èŒãããé²åŸ¡å¹æã¯æ¬¡åŒã«ããç®åºããã é²åŸ¡å¹æïŒïŒ ïŒ ïŒè©ŠéšçŸ€ã®çæ®å¹æ°âå¯Ÿç §çŸ€ã®çæ®å¹æ°ïŒïŒçŸ€ã®ããŠ
ã¹æ°Ã100 çµæã¯äžè¡šã«ç€ºãéãã§ãããRDPç©è³ªã®10
ã100mgïŒKgã®äºåæäžã«ããããšã·ãšãªãã¢ã»
ã³ãªææã«å¯ŸããŠã¯éåžžã«åŒ·ãé²åŸ¡äœçšãçãã
ãªã¹ããªã¢ã»ã¢ããµã€ãã²ãã¹ææã«å¯ŸããŠãæ
æãªé²åŸ¡äœçšã®å¢åŒ·å¹æãã¿ãããã åŸè¿°ããããã«ãRDPç©è³ªã¯æ¯æ§ãå šãèŠã
ããªãç¹ãšãåãããŠã極ããŠæå¹ãªææçäºé²
æ²»çå€ãšãªããããšèããããã
ãè¡šã
次ã«ãRDPç©è³ªã®æ¥æ§æ¯æ§ã«ã€ããŠèšåããã
ïŒé±ä»€ãªã¹ã®SDâCRJã©ãããäœé120ã150ïœã
ïŒçŸ€10å¹ãçšããŠRDPç©è³ªã®ç©ççæäžéçã§
ãã15ïœïŒKgãæäžã芳å¯ãç¶ãããšãããå šäŸ
æ»äº¡äŸããªãäœéå¢å ãå¯Ÿç §ãšå€ããããããã
å€èŠ³äžãåæ€äžãå šãç°åžžãèªããããªãã€ãã
ãããã€ãŠãLD50ïŒ15ïœïŒKgãšèããããæ¥æ§
æ¯æ§ã¯ãªããã®ãšå€æãããã ãã®ããã«åªããæè «ç掻æ§ãå ç«èª¿ç¯æŽ»æ§ã
ææé²åŸ¡æŽ»æ§ã瀺ãå€ç³äœRDPç©è³ªãæ¯èŒç容
æãªæäœã®çµã¿åããã«ããäžèšè£œé äŸã«ç€ºãã
ãããã«å€§éã«åŸãããã®ã§ãç±³ç³ ããçç掻æ§
å€ç³ãå·¥æ¥çã«è£œé ããæè¡äžã«äžããå¹æã¯é
åžžã«å€§ãªããã®ã§ããã ããã«ã€ã³ã¿ãŒããšãã³èªèµ·èœãã¿ãããããš
ãããã«ãã¹ãã€ã³ãã«ãšã³ã¶çã®ãŠã€ã«ã¹æ§çŸ
æ£ã«å¯Ÿããäºé²æ²»çå¹æãæåŸ ã§ããã RDPç©è³ªã¯çµå£çãŸãã¯éçµå£çã«æäžã§ã
ãã®ã§ã極ããŠæçšãªæè «çå€ãå ç«èª¿ç¯å€ãã
ãã¯ææçäºé²æ²»çå€ãšããŠæåŸ ãããã ãªããå®éã®è£œå€åã«ã€ããŠã¯ãæ¬ç©è³ªãåç¬
ã§ããããã¯è³Šåœ¢å€ïŒæ°Žãççé£å¡©æ°Žãããªãšã
ã¬ã³ã°ãªã³ãŒã«ãã°ãªã»ããŒã©ãã³ã柱ç²ããã
ã¹ããªã³ãä¹³ç³ãªã©ïŒãšçµã¿åãããŠæ°Žå€ãäžž
å€ãé å€ãæ£å€ãåå€ãªã©ã®å€åã«ãŠè£œé ããã
ãšãã§ããã 次ã«ãæ¬ç©è³ªã®è£œé ã以äžã®è£œé äŸã«ãã€ãŠèª¬
æããã 補é äŸ ïŒ åžè²©ã®ç±³ç³ ãçšã篩ã§ç ç±³çãé€ãããã®25Kg
ã«æ°Žéæ°Ž125ãå ãã120âã§ïŒæéããã®åŸã
ãã«100âã§ïŒæéå ç±ãæ¹æããªããæœåºãè¡
ãªã€ãã æœåºæ¶²ãæ¿ŸéåŸã40ã«æžå§æ¿çž®ããæ°Žé žåã
ããªãŠã ã§PHã6.7ãšããåŸã500mgã®Î±âã¢ãã©
ãŒãŒïŒé·ç¬ç£æ¥è£œïŒãå ãã70âã§ïŒæéé µçŽ åŠ
çãè¡ãªã€ããåå¿åŸã100âãŸã§å ç±ãé µçŽ ã
倱掻ãããé å¿åé¢ã«ããäžæº¶ç©ãé€å»ããæçµ
æ¿åºŠã30ïŒ ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ãšã¿ããŒã«ã
å ããŠãçããæ²ã§ããåé¢ãããåé¢åŸãæ°Žã«
溶解ããããŠäžæº¶ç©ãé€ããåçµä¹Ÿç¥ãè¡ãªã€ã
ãšããã508ïœã®æ·¡é»è²ç²æ«ãåŸãããã®æ·¡é»è²
ç²æ«ïŒïœãå床ã€ãªã³äº€ææ°Žã«æº¶ãããäžæº¶ç©ã
é å¿åé¢ã§é€å»ããåŸãã»ãã¢ããŒãºCLâ6B
ïŒãã¢ã«ãã·ã¢è£œïŒã«ããã²ã«æ¿Ÿéãããã®ãã€
ãããªãŠãŒã ã«æº¶åºãããç»åãéããŠåçµä¹Ÿç¥
ãè¡ãªããïŒïœã®çœè²ç²æ«ãåŸãããããããã«
100mlã®ã€ãªã³äº€ææ°Žã«æº¶è§£ãããã¯ãããã«ã
20mlãïœâãã¿ããŒã«ïŒmlãšãšãã«å液濟æãçš
ããŠ60åéæ¯ãšãããäœéã§é å¿ãããšæ°Žå±€ãšã¯
ãããã«ã å±€ãšã®éã«å€æ§èçœè³ªã®çœãå±€ãã§ã
ããæ°Žå±€éšåãåãåºããåã³åæ¯çã®ã¯ããã
ã«ã ããã³ïœâãã¿ããŒã«ãå ããŠæ¯ãšãããã
ãã®æäœãå€æ§èçœè³ªã®çœãå±€ãåºãªããªããŸã§
çŽ30åç¹°ãè¿ããåŸãæ°Žå±€éšåãåçµä¹Ÿç¥ãããŠ
800mgã®çœè²ã®é€èçœãããç²æ«ãåŸãã 補é äŸ ïŒ åžè²©ã®ç±³ç³ 25Kgããããµã³100ã§éæµè±èã
ãã®ã¡ä¹Ÿç¥ããç±³ç³ ã補é äŸïŒãšåæ§ã®æ¹æ³ã§æœ
åºåååŠçãããŠæ·¡é»è²ç²æ«450ïœãåŸãããã®
ç²æ«ïŒïœãçšããŠè£œé äŸïŒãšåæ§ã®æ¹æ³ã§åŠçã
ãŠ750mgã®çœè²ç²æ«ãåŸãã 補é äŸ ïŒ åžè²©ã®è±èç³ ïŒKgã«æ°Ž20ãå ãæ¹æããªãã
120âã§ïŒæéå å§å ç±äžã§æœåºãããæœåºæ¶²ã
æžå§æ¿çž®ããŠåŸãïŒã®æ¿çž®æ¶²ã«çµæ¶Î±âã¢ãã©
ãŒãŒïŒé·ç¬ç£æ¥è£œïŒ0.3ïœãå ããŠ60âã§ïŒæé
ä¿æããããããåŸã100âã«å æž©ããåŸãé å¿
åé¢ããŠäžæž 4.9ãåŸãããã®äžæž ã«ãšã¿ããŒ
ã«ãå ããŠãšã¿ããŒã«æ¿åºŠ40ïŒ ãšããçããæ²ã§
ããååããã次ãã§ãããåçµä¹Ÿç¥ããŠæ·¡é»è€
è²ç²æ«88ïœãåŸãã ãã®ç²æ«ïŒïœãçšããŠè£œé äŸïŒãšåæ§ã«åŠçã
ãŠ720mgã®çœè²ç²æ«ãåŸãã 補é äŸ ïŒ åžè²©ã®ç±³ç³ 20Kgã30ã¡ãã·ãŠã®ç¯©ã§ãµãã€ãŠç
ç±³çã®å€Ÿéç©ãé€å»ããåŸãã€ãªã³äº€ææš¹èã§åŠ
çããæ°Žéæ°Ž100ã§æŽæµããã次ãã§ãæŽæµã
ãç±³ç³ ã«èžçæ°Ž50ãå ãæ¹æããªãã110âã§
ïŒæéå å§å ç±äžã§æœåºããåŸãæ¿ŸéãããåŸã
ãã濟液ãæžå§æ¿çž®ããããã«é å¿åé¢ããŠäžæž
10ãåŸãããã®äžæž ã«çµæ¶Î±âã¢ãã©ãŒãŒ250
mgãæ·»å ãã65âã§24æéäœçšããããåŸã100
âã«å æž©ããã次ãã§ãšã¿ããŒã«æ¿åºŠ30ïŒ ã«ãªã
ããã«ãšã¿ããŒã«ãå ãçããæ²ã§ããé å¿åé¢
ã«ãã€ãŠæ¡åããããã®æ²ã§ãã«æ°ŽïŒãå ããŠ
溶解ããåŸãé å¿åé¢ãè¡ãªã€ãŠäžæž ãåŸããã
ã®äžæž ãïŒãšãªããŸã§æžå§æ¿çž®ããããã«é å¿
åé¢ããŠäžæž ãåŸããããæµæ°Žã«å¯ŸããŠïŒæ¥éé
æããé å¿åé¢ãè¡ãªã€ãŠäžæž ïŒãåŸãããã®
äžæž ïŒã«ã¯ãããã«ã 200mlãïœâãã¿ããŒã«
40mlãå ãã以äžè£œé äŸïŒãšåæ§ã®æ¹æ³ã§é€èçœ
åŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœè²ç²æ«402
ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§éæåŸé å¿åé¢ããŠåŸãããäžæž ïŒ
ãéœã€ãªã³äº€æã²ã«ã§ããCMã»ãã¢ããŒãº
ïŒãã¢ã«ãã·ã¢è£œïŒããã³é°ã€ãªã³äº€æã²ã«ã§ã
ãDEAEã»ãã¢ããŒãºïŒãã¢ã«ãã·ã¢è£œïŒã§é 次
ã€ãªã³äº€æåŠçããéåžçéšãéãïŒã«æ¿çž®ã
ãåŸã補é äŸïŒãšåæ§ã®æ¹æ³ã§é€èçœåŠçããã³
åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœè²ç²æ«358ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§éæåŸé å¿åé¢ããŠåŸãããäžæž ïŒ
ã«æŽ»æ§ç10ïœãå ãã30ååŸã«é å¿åé¢ãè¡ãª
ãäžæž ãåŸãããã®äžæž ã補é äŸïŒãšåæ§ã®æ¹æ³
ã§é€èçœåŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœè²
ç²æ«365ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§éæåŸé å¿åé¢ããŠåŸãããäžæž ïŒ
ã®ãã¡20mlãã»ãã¢ããŒãºCLâ6BïŒãã¢ã«ã
ã·ã¢è£œïŒã§ã²ã«æ¿Ÿéãããã®ãã€ãããªãŠãŒã ç»
åãéããŠ100mlãšããããã®æ¶²ã補é äŸïŒãšå
æ§ã«é€èçœåŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœ
è²ç²æ«70ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçç©ããšã¿ããŒã«
æ²ã§ããããŠåŸãããæ²ã§ãç©ãåã³10ã®æ°Žã«
溶解ããåç»ååéïŒäžã®ãŠã«ãã©ãã€ã«ã¿ãŒè
ãçšããŠäœååç»åãé€å»ãããšåæã«ïŒã«ãª
ããŸã§æ¿çž®ããçããæ²ã§ããé å¿åé¢ããŠé€å»
ã2.8ã®äžæž ãåŸãããã®äžæž ã補é äŸïŒãšå
æ§ã«é€èçœåŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠ
400ïœã®çœè²ç²æ«ãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçã100âã§ïŒæ
éå ç±ããŠé µçŽ ã倱掻ããã液ã«ãæçµæ¿åºŠ40ïŒ
ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ã¢ã»ãã³ãå ããçãã
æ²ã§ãã10ã®æ°Žã«æº¶è§£ããã以åŸã補é äŸïŒã«
ããããŠã«ãã©ãã€ã«ã¿ãŒèãçšããåŠç以åŸã®
å·¥çšãè¡ãªã412ïœã®çœè²ç²æ«ãåŸãã 補é äŸ 10 補é äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçã100âã§ïŒæ
éå ç±ããŠé µçŽ ã倱掻ããã液ã«ã飜å床70ïŒ ã«
ãªãããã«ç¡«é žã¢ã³ã¢ããŠã ãå ããŠå¡©æãè¡ãª
ããçããæ²ã§ããé å¿åé¢ã§éãïŒã®æ°Žã«æº¶
解ããæµæ°Žã«å¯ŸããŠïŒæ¥ééæãè¡ãªã€ãããã®
éæå 液ã«ããªã¯ãã«é ¢é žãæ¿åºŠïŒïŒ ã«ãªããã
ã«æ·»å ããŠæ²ã§ããçããããçããæ²ã§ããé
å¿åé¢ã§é€å»ããäžæž ãåã³æµæ°Žã«å¯ŸããŠïŒæ¥é
éæããéæå 液ãåçµä¹Ÿç¥ããŠæ·¡é»è²ç²æ«503
ïœãåŸãããã®ç²æ«ïŒïœãã€ãªã³äº€ææ°Žã«æº¶è§£
ããã»ãã¢ããŒãºCLâ6BïŒãã¢ã«ãã·ã¢è£œïŒã®
ã«ã©ã ã§ã²ã«æ¿Ÿéãè¡ãªãããã®ãã€ãããªãŠãŒ
ã ç»åãéããŠåçµä¹Ÿç¥ãçœè²ç²æ«1.5ïœãåŸãã 補é äŸ 11 補é äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçãè¡ãªã€ã液ã
ãã®ãŸãŸ40âãŸã§äžããèçœåè§£é µçŽ ïŒããããŒ
ãŒïŒ¥ãç§ç ååŠè£œïŒ600mgãå ããŠ24æéåå¿ã
ãããåå¿æ¶²ã100âã§ïŒæéå ç±ããŠé µçŽ ã倱
掻ãããåŸãé å¿åé¢ããŠäžæº¶ç©ãé€å»ããäžæž
ã«ãæçµæ¿åºŠ30ïŒ ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ãšã¿ã
ãŒã«ãå ããçããæ²ã§ããé å¿åé¢ã§éãã10
ã®æ°Žã«æº¶è§£ããã以åŸã補é äŸïŒã«ããããŠã«
ãã©ãã€ã«ã¿ãŒèãçšããåŠç以åŸã®å·¥çšãè¡ãª
ãçœè²ç²æ«413ïœãåŸãã 補é äŸ 12 補é äŸïŒãšåæ§ã®å·¥çšãçµãŠåŸããããŠã«ãã©
ãã€ã«ã¿ãŒèåŠçäžæž ïŒã«è£œé äŸïŒãšåæ§ã®æ¯
çã§ã¯ãããã«ã ãïœâãã¿ããŒã«ãå ããŠé€è
çœæäœãè¡ãªãã氎溶éšãåŽé§ä¹Ÿç¥ããŠ420ïœã®
çœè²ç²æ«ãåŸãã 補é äŸ 13 補é äŸ12ã§é€èçœæäœãè¡ãªã€ãŠåŸããã氎溶
éšã«æçµæ¿åºŠ40ïŒ ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ãšã¿ã
ãŒã«ãå ããçããæ²ã§ããé å¿åé¢ã§éããã
ãã«ïŒåãšã¿ããŒã«ã§æŽæµè±æ°ŽããåŸãç空也ç¥
ããŠçœè²ç²æ«405ïœãåŸãã
ïŒé±ä»€ãªã¹ã®SDâCRJã©ãããäœé120ã150ïœã
ïŒçŸ€10å¹ãçšããŠRDPç©è³ªã®ç©ççæäžéçã§
ãã15ïœïŒKgãæäžã芳å¯ãç¶ãããšãããå šäŸ
æ»äº¡äŸããªãäœéå¢å ãå¯Ÿç §ãšå€ããããããã
å€èŠ³äžãåæ€äžãå šãç°åžžãèªããããªãã€ãã
ãããã€ãŠãLD50ïŒ15ïœïŒKgãšèããããæ¥æ§
æ¯æ§ã¯ãªããã®ãšå€æãããã ãã®ããã«åªããæè «ç掻æ§ãå ç«èª¿ç¯æŽ»æ§ã
ææé²åŸ¡æŽ»æ§ã瀺ãå€ç³äœRDPç©è³ªãæ¯èŒç容
æãªæäœã®çµã¿åããã«ããäžèšè£œé äŸã«ç€ºãã
ãããã«å€§éã«åŸãããã®ã§ãç±³ç³ ããçç掻æ§
å€ç³ãå·¥æ¥çã«è£œé ããæè¡äžã«äžããå¹æã¯é
åžžã«å€§ãªããã®ã§ããã ããã«ã€ã³ã¿ãŒããšãã³èªèµ·èœãã¿ãããããš
ãããã«ãã¹ãã€ã³ãã«ãšã³ã¶çã®ãŠã€ã«ã¹æ§çŸ
æ£ã«å¯Ÿããäºé²æ²»çå¹æãæåŸ ã§ããã RDPç©è³ªã¯çµå£çãŸãã¯éçµå£çã«æäžã§ã
ãã®ã§ã極ããŠæçšãªæè «çå€ãå ç«èª¿ç¯å€ãã
ãã¯ææçäºé²æ²»çå€ãšããŠæåŸ ãããã ãªããå®éã®è£œå€åã«ã€ããŠã¯ãæ¬ç©è³ªãåç¬
ã§ããããã¯è³Šåœ¢å€ïŒæ°Žãççé£å¡©æ°Žãããªãšã
ã¬ã³ã°ãªã³ãŒã«ãã°ãªã»ããŒã©ãã³ã柱ç²ããã
ã¹ããªã³ãä¹³ç³ãªã©ïŒãšçµã¿åãããŠæ°Žå€ãäžž
å€ãé å€ãæ£å€ãåå€ãªã©ã®å€åã«ãŠè£œé ããã
ãšãã§ããã 次ã«ãæ¬ç©è³ªã®è£œé ã以äžã®è£œé äŸã«ãã€ãŠèª¬
æããã 補é äŸ ïŒ åžè²©ã®ç±³ç³ ãçšã篩ã§ç ç±³çãé€ãããã®25Kg
ã«æ°Žéæ°Ž125ãå ãã120âã§ïŒæéããã®åŸã
ãã«100âã§ïŒæéå ç±ãæ¹æããªããæœåºãè¡
ãªã€ãã æœåºæ¶²ãæ¿ŸéåŸã40ã«æžå§æ¿çž®ããæ°Žé žåã
ããªãŠã ã§PHã6.7ãšããåŸã500mgã®Î±âã¢ãã©
ãŒãŒïŒé·ç¬ç£æ¥è£œïŒãå ãã70âã§ïŒæéé µçŽ åŠ
çãè¡ãªã€ããåå¿åŸã100âãŸã§å ç±ãé µçŽ ã
倱掻ãããé å¿åé¢ã«ããäžæº¶ç©ãé€å»ããæçµ
æ¿åºŠã30ïŒ ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ãšã¿ããŒã«ã
å ããŠãçããæ²ã§ããåé¢ãããåé¢åŸãæ°Žã«
溶解ããããŠäžæº¶ç©ãé€ããåçµä¹Ÿç¥ãè¡ãªã€ã
ãšããã508ïœã®æ·¡é»è²ç²æ«ãåŸãããã®æ·¡é»è²
ç²æ«ïŒïœãå床ã€ãªã³äº€ææ°Žã«æº¶ãããäžæº¶ç©ã
é å¿åé¢ã§é€å»ããåŸãã»ãã¢ããŒãºCLâ6B
ïŒãã¢ã«ãã·ã¢è£œïŒã«ããã²ã«æ¿Ÿéãããã®ãã€
ãããªãŠãŒã ã«æº¶åºãããç»åãéããŠåçµä¹Ÿç¥
ãè¡ãªããïŒïœã®çœè²ç²æ«ãåŸãããããããã«
100mlã®ã€ãªã³äº€ææ°Žã«æº¶è§£ãããã¯ãããã«ã
20mlãïœâãã¿ããŒã«ïŒmlãšãšãã«å液濟æãçš
ããŠ60åéæ¯ãšãããäœéã§é å¿ãããšæ°Žå±€ãšã¯
ãããã«ã å±€ãšã®éã«å€æ§èçœè³ªã®çœãå±€ãã§ã
ããæ°Žå±€éšåãåãåºããåã³åæ¯çã®ã¯ããã
ã«ã ããã³ïœâãã¿ããŒã«ãå ããŠæ¯ãšãããã
ãã®æäœãå€æ§èçœè³ªã®çœãå±€ãåºãªããªããŸã§
çŽ30åç¹°ãè¿ããåŸãæ°Žå±€éšåãåçµä¹Ÿç¥ãããŠ
800mgã®çœè²ã®é€èçœãããç²æ«ãåŸãã 補é äŸ ïŒ åžè²©ã®ç±³ç³ 25Kgããããµã³100ã§éæµè±èã
ãã®ã¡ä¹Ÿç¥ããç±³ç³ ã補é äŸïŒãšåæ§ã®æ¹æ³ã§æœ
åºåååŠçãããŠæ·¡é»è²ç²æ«450ïœãåŸãããã®
ç²æ«ïŒïœãçšããŠè£œé äŸïŒãšåæ§ã®æ¹æ³ã§åŠçã
ãŠ750mgã®çœè²ç²æ«ãåŸãã 補é äŸ ïŒ åžè²©ã®è±èç³ ïŒKgã«æ°Ž20ãå ãæ¹æããªãã
120âã§ïŒæéå å§å ç±äžã§æœåºãããæœåºæ¶²ã
æžå§æ¿çž®ããŠåŸãïŒã®æ¿çž®æ¶²ã«çµæ¶Î±âã¢ãã©
ãŒãŒïŒé·ç¬ç£æ¥è£œïŒ0.3ïœãå ããŠ60âã§ïŒæé
ä¿æããããããåŸã100âã«å æž©ããåŸãé å¿
åé¢ããŠäžæž 4.9ãåŸãããã®äžæž ã«ãšã¿ããŒ
ã«ãå ããŠãšã¿ããŒã«æ¿åºŠ40ïŒ ãšããçããæ²ã§
ããååããã次ãã§ãããåçµä¹Ÿç¥ããŠæ·¡é»è€
è²ç²æ«88ïœãåŸãã ãã®ç²æ«ïŒïœãçšããŠè£œé äŸïŒãšåæ§ã«åŠçã
ãŠ720mgã®çœè²ç²æ«ãåŸãã 補é äŸ ïŒ åžè²©ã®ç±³ç³ 20Kgã30ã¡ãã·ãŠã®ç¯©ã§ãµãã€ãŠç
ç±³çã®å€Ÿéç©ãé€å»ããåŸãã€ãªã³äº€ææš¹èã§åŠ
çããæ°Žéæ°Ž100ã§æŽæµããã次ãã§ãæŽæµã
ãç±³ç³ ã«èžçæ°Ž50ãå ãæ¹æããªãã110âã§
ïŒæéå å§å ç±äžã§æœåºããåŸãæ¿ŸéãããåŸã
ãã濟液ãæžå§æ¿çž®ããããã«é å¿åé¢ããŠäžæž
10ãåŸãããã®äžæž ã«çµæ¶Î±âã¢ãã©ãŒãŒ250
mgãæ·»å ãã65âã§24æéäœçšããããåŸã100
âã«å æž©ããã次ãã§ãšã¿ããŒã«æ¿åºŠ30ïŒ ã«ãªã
ããã«ãšã¿ããŒã«ãå ãçããæ²ã§ããé å¿åé¢
ã«ãã€ãŠæ¡åããããã®æ²ã§ãã«æ°ŽïŒãå ããŠ
溶解ããåŸãé å¿åé¢ãè¡ãªã€ãŠäžæž ãåŸããã
ã®äžæž ãïŒãšãªããŸã§æžå§æ¿çž®ããããã«é å¿
åé¢ããŠäžæž ãåŸããããæµæ°Žã«å¯ŸããŠïŒæ¥éé
æããé å¿åé¢ãè¡ãªã€ãŠäžæž ïŒãåŸãããã®
äžæž ïŒã«ã¯ãããã«ã 200mlãïœâãã¿ããŒã«
40mlãå ãã以äžè£œé äŸïŒãšåæ§ã®æ¹æ³ã§é€èçœ
åŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœè²ç²æ«402
ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§éæåŸé å¿åé¢ããŠåŸãããäžæž ïŒ
ãéœã€ãªã³äº€æã²ã«ã§ããCMã»ãã¢ããŒãº
ïŒãã¢ã«ãã·ã¢è£œïŒããã³é°ã€ãªã³äº€æã²ã«ã§ã
ãDEAEã»ãã¢ããŒãºïŒãã¢ã«ãã·ã¢è£œïŒã§é 次
ã€ãªã³äº€æåŠçããéåžçéšãéãïŒã«æ¿çž®ã
ãåŸã補é äŸïŒãšåæ§ã®æ¹æ³ã§é€èçœåŠçããã³
åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœè²ç²æ«358ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§éæåŸé å¿åé¢ããŠåŸãããäžæž ïŒ
ã«æŽ»æ§ç10ïœãå ãã30ååŸã«é å¿åé¢ãè¡ãª
ãäžæž ãåŸãããã®äžæž ã補é äŸïŒãšåæ§ã®æ¹æ³
ã§é€èçœåŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœè²
ç²æ«365ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§éæåŸé å¿åé¢ããŠåŸãããäžæž ïŒ
ã®ãã¡20mlãã»ãã¢ããŒãºCLâ6BïŒãã¢ã«ã
ã·ã¢è£œïŒã§ã²ã«æ¿Ÿéãããã®ãã€ãããªãŠãŒã ç»
åãéããŠ100mlãšããããã®æ¶²ã補é äŸïŒãšå
æ§ã«é€èçœåŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠçœ
è²ç²æ«70ïœãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçç©ããšã¿ããŒã«
æ²ã§ããããŠåŸãããæ²ã§ãç©ãåã³10ã®æ°Žã«
溶解ããåç»ååéïŒäžã®ãŠã«ãã©ãã€ã«ã¿ãŒè
ãçšããŠäœååç»åãé€å»ãããšåæã«ïŒã«ãª
ããŸã§æ¿çž®ããçããæ²ã§ããé å¿åé¢ããŠé€å»
ã2.8ã®äžæž ãåŸãããã®äžæž ã補é äŸïŒãšå
æ§ã«é€èçœåŠçããã³åçµä¹Ÿç¥åŠçãè¡ãªã€ãŠ
400ïœã®çœè²ç²æ«ãåŸãã 補é äŸ ïŒ è£œé äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçã100âã§ïŒæ
éå ç±ããŠé µçŽ ã倱掻ããã液ã«ãæçµæ¿åºŠ40ïŒ
ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ã¢ã»ãã³ãå ããçãã
æ²ã§ãã10ã®æ°Žã«æº¶è§£ããã以åŸã補é äŸïŒã«
ããããŠã«ãã©ãã€ã«ã¿ãŒèãçšããåŠç以åŸã®
å·¥çšãè¡ãªã412ïœã®çœè²ç²æ«ãåŸãã 補é äŸ 10 補é äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçã100âã§ïŒæ
éå ç±ããŠé µçŽ ã倱掻ããã液ã«ã飜å床70ïŒ ã«
ãªãããã«ç¡«é žã¢ã³ã¢ããŠã ãå ããŠå¡©æãè¡ãª
ããçããæ²ã§ããé å¿åé¢ã§éãïŒã®æ°Žã«æº¶
解ããæµæ°Žã«å¯ŸããŠïŒæ¥ééæãè¡ãªã€ãããã®
éæå 液ã«ããªã¯ãã«é ¢é žãæ¿åºŠïŒïŒ ã«ãªããã
ã«æ·»å ããŠæ²ã§ããçããããçããæ²ã§ããé
å¿åé¢ã§é€å»ããäžæž ãåã³æµæ°Žã«å¯ŸããŠïŒæ¥é
éæããéæå 液ãåçµä¹Ÿç¥ããŠæ·¡é»è²ç²æ«503
ïœãåŸãããã®ç²æ«ïŒïœãã€ãªã³äº€ææ°Žã«æº¶è§£
ããã»ãã¢ããŒãºCLâ6BïŒãã¢ã«ãã·ã¢è£œïŒã®
ã«ã©ã ã§ã²ã«æ¿Ÿéãè¡ãªãããã®ãã€ãããªãŠãŒ
ã ç»åãéããŠåçµä¹Ÿç¥ãçœè²ç²æ«1.5ïœãåŸãã 補é äŸ 11 補é äŸïŒã§Î±âã¢ãã©ãŒãŒåŠçãè¡ãªã€ã液ã
ãã®ãŸãŸ40âãŸã§äžããèçœåè§£é µçŽ ïŒããããŒ
ãŒïŒ¥ãç§ç ååŠè£œïŒ600mgãå ããŠ24æéåå¿ã
ãããåå¿æ¶²ã100âã§ïŒæéå ç±ããŠé µçŽ ã倱
掻ãããåŸãé å¿åé¢ããŠäžæº¶ç©ãé€å»ããäžæž
ã«ãæçµæ¿åºŠ30ïŒ ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ãšã¿ã
ãŒã«ãå ããçããæ²ã§ããé å¿åé¢ã§éãã10
ã®æ°Žã«æº¶è§£ããã以åŸã補é äŸïŒã«ããããŠã«
ãã©ãã€ã«ã¿ãŒèãçšããåŠç以åŸã®å·¥çšãè¡ãª
ãçœè²ç²æ«413ïœãåŸãã 補é äŸ 12 補é äŸïŒãšåæ§ã®å·¥çšãçµãŠåŸããããŠã«ãã©
ãã€ã«ã¿ãŒèåŠçäžæž ïŒã«è£œé äŸïŒãšåæ§ã®æ¯
çã§ã¯ãããã«ã ãïœâãã¿ããŒã«ãå ããŠé€è
çœæäœãè¡ãªãã氎溶éšãåŽé§ä¹Ÿç¥ããŠ420ïœã®
çœè²ç²æ«ãåŸãã 補é äŸ 13 補é äŸ12ã§é€èçœæäœãè¡ãªã€ãŠåŸããã氎溶
éšã«æçµæ¿åºŠ40ïŒ ïŒïŒ¶ïŒïŒ¶ïŒãšãªãããã«ãšã¿ã
ãŒã«ãå ããçããæ²ã§ããé å¿åé¢ã§éããã
ãã«ïŒåãšã¿ããŒã«ã§æŽæµè±æ°ŽããåŸãç空也ç¥
ããŠçœè²ç²æ«405ïœãåŸãã
第ïŒå³ã¯çç掻æ§å€ç³äœRDPç©è³ªã®çŽ«å€éšåž
åã¹ãã¯ãã«ã®ç¹æ§åžåå³ã§ããã第ïŒå³ã¯åã
ãèµ€å€éšåžåã¹ãã¯ãã«ã®ç¹æ§åžåå³ã§ããã第
ïŒå³ã¯åãã13CâNMRã¹ãã¯ãã«ã®ç¹æ§åžå
å³ã§ããã
åã¹ãã¯ãã«ã®ç¹æ§åžåå³ã§ããã第ïŒå³ã¯åã
ãèµ€å€éšåžåã¹ãã¯ãã«ã®ç¹æ§åžåå³ã§ããã第
ïŒå³ã¯åãã13CâNMRã¹ãã¯ãã«ã®ç¹æ§åžå
å³ã§ããã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ ç±³ç³ ãç±æ°Žæœåºããæœåºç©ãããã«é€èçœæ
äœããããšã«ããåŸããã以äžã®æ§è³ªãæããæ°
èŠå€ç³äœRDPç©è³ªã (1) ååéïŒéæèãééãããååéïŒäžä»¥äž
ãšæšå®ãããã (2) 溶解æ§ïŒã¢ã«ã³ãŒã«ãã¢ã»ãã³ããããµã³ã
ãã³ãŒã³ãé ¢é žãšãã«ããžã¡ãã«ã¹ã«ãã
ã·ãããªã°ãã€ã³ãåå¡©åççŽ ãã¯ããã
ã«ã ããã³ãšãŒãã«ã«äžæº¶ã§ãããæ°Žã«ã¯
å¯æº¶ã§ããã (3) 氎溶液ã®PHïŒïŒïŒ 氎溶液ã¯äžæ§ã瀺ãã (4) èçœè³ªãç¡æ©è³ªïŒèçœè³ªãå«æãããïŒïŒ 以
äžã®ç¡æ©è³ªãå«æããã (5) åè²åå¿ïŒã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«
é žåå¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ã
ã€ã³ç¡«é žåå¿ãã¯ãã¢ãããŒãç¡«é žåå¿ã
ããšããŒã«ç¡«é žåå¿ãã«ã«ããŸãŒã«ç¡«é žå
å¿ãéœæ§ã§ãããããŠãŒã¬ããåå¿ããã³
ãããªã³åå¿ãããŒãªãŒããªãŒãªã³åå¿ã
ãšã«ãœã«ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ãé°æ§
ã§ããã (6) 玫å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã玫å€éš
åžåã¹ãã¯ãã«ã瀺ãã (7) èµ€å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºãèµ€å€éš
åžåã¹ãã¯ãã«ã瀺ãã (8) 13CâNMRã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã13Câ
NMRã¹ãã¯ãã«ã瀺ãã (9) æ§æç³ïŒã°ã«ã³ãŒã¹ã®ã¿ã§æ§æãããã (10) æ§é ïŒÎ±âïŒïŒïŒã°ã«ã³ã·ãçµåãäž»éªšæ Œãš
ããçŽ10åã®Î±âïŒïŒïŒçµåã§çµã°ããã°
ã«ã³ãŒã¹æ®åºã«ïŒåã®å²åã§Î±âïŒïŒïŒçµ
åãæããã (11) èç¹ïŒäžå®ã®èç¹ã瀺ããã220âã§è€å€ãã
280âã§é»å€ããŠçåãèµ·ããã ïŒ ç±³ç³ ãç±æ°ŽåŠçããŠåŸãããæœåºæ¶²ã«æ¥µæ§æ
æ©æº¶åªãŸãã¯å¡©æå€ãå ããçããæ²ã§ããåå
ããé€èçœæäœãè¡ãªãããšãç¹åŸŽãšãã以äžã®
æ§è³ªãæããæ°èŠå€ç³äœRDPç©è³ªã®è£œé æ³ã (1) ååéïŒéæèãééãããååéïŒäžä»¥äž
ãšæšå®ãããã (2) 溶解æ§ïŒã¢ã«ã³ãŒã«ãã¢ã»ãã³ããããµã³ã
ãã³ãŒã³ãé ¢é žãšãã«ããžã¡ãã«ã¹ã«ãã
ã·ãããªã°ãã€ã³ãåå¡©åççŽ ãã¯ããã
ã«ã ããã³ãšãŒãã«ã«äžæº¶ã§ãããæ°Žã«ã¯
å¯æº¶ã§ããã (3) 氎溶液ã®PHïŒïŒïŒ 氎溶液ã¯äžæ§ã瀺ãã (4) èçœè³ªãç¡æ©è³ªïŒèçœè³ªãå«æãããïŒïŒ 以
äžã®ç¡æ©è³ªãå«æããã (5) åè²åå¿ïŒã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«
é žåå¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ã
ã€ã³ç¡«é žåå¿ãã¯ãã¢ãããŒãç¡«é žåå¿ã
ããšããŒã«ç¡«é žåå¿ãã«ã«ããŸãŒã«ç¡«é žå
å¿ãéœæ§ã§ãããããŠãŒã¬ããåå¿ããã³
ãããªã³åå¿ãããŒãªãŒããªãŒãªã³åå¿ã
ãšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ãé°æ§
ã§ããã (6) 玫å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã玫å€éš
åžåã¹ãã¯ãã«ã瀺ãã (7) èµ€å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºãèµ€å€éš
åžåã¹ãã¯ãã«ã瀺ãã (8) 13CâNMRã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã13Câ
NMRã¹ãã¯ãã«ã瀺ãã (9) æ§æç³ïŒã°ã«ã³ãŒã¹ã®ã¿ã§æ§æãããã (10) æ§é ïŒÎ±âïŒïŒïŒã°ã«ã³ã·ãçµåãäž»éªšæ Œãš
ããçŽ10åã®Î±âïŒïŒïŒçµåã§çµã°ããã°
ã«ã³ãŒã¹æ®åºã«ïŒåã®å²åã§Î±âïŒïŒïŒçµ
åãæããã (11) èç¹ïŒäžå®ã®èç¹ã瀺ããã220âã§è€å€ãã
280âã§é»å€ããŠçåãèµ·ããã ïŒ æ¥µæ§ææ©æº¶åªãã¡ã¿ããŒã«ããšã¿ããŒã«ãã
ããããŒã«ããã³ã¢ã»ãã³ã®ããããã§ããç¹èš±
è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ°èŠå€ç³äœRDPç©è³ªã®
補é æ³ã ïŒ å¡©æå€ãå¡©åãããªãŠã ãç¡«é žã¢ã³ã¢ããŠã
ããã³å¡©åã«ãªãŠã ã®ããããã§ããç¹èš±è«æ±ã®
ç¯å²ç¬¬ïŒé èšèŒã®æ°èŠå€ç³äœRDPç©è³ªã®è£œé æ³ã ïŒ é€èçœæäœãé µçŽ åŠçæ³ãé žåŠçæ³ããã³ã»
ããŒã°æ³ã®ããããã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé
èšèŒã®æ°èŠå€ç³äœRDPç©è³ªã®è£œé æ³ã ïŒ ç±³ç³ ãç±æ°Žæœåºããæœåºç©ãããã«é€èçœæ
äœããããšã«ããåŸããã以äžã®æ§è³ªãæããæ°
èŠå€ç³äœRDPç©è³ªãŸãã¯è©²ç©è³ªãšè£œå€äžèš±å®¹ã
ãã賊圢å€ããå®è³ªçã«ãªããã®ãæå¹æåãšã
ãæè «çå€ã (1) ååéïŒéæèãééãããååéïŒäžä»¥äž
ãšæšå®ãããã (2) 溶解æ§ïŒã¢ã«ã³ãŒã«ãã¢ã»ãã³ããããµã³ã
ãã³ãŒã³ãé ¢é žãšãã«ããžã¡ãã«ã¹ã«ãã
ã·ãããªã°ãã€ã³ãåå¡©åççŽ ãã¯ããã
ã«ã ããã³ãšãŒãã«ã«äžæº¶ã§ãããæ°Žã«ã¯
å¯æº¶ã§ããã (3) 氎溶液ã®PHïŒïŒïŒ 氎溶液ã¯äžæ§ã瀺ãã (4) èçœè³ªãç¡æ©è³ªïŒèçœè³ªãå«æãããïŒïŒ 以
äžã®ç¡æ©è³ªãå«æããã (5) åè²åå¿ïŒã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«
é žåå¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ã
ã€ã³ç¡«é žåå¿ãã¯ãã¢ãããŒãç¡«é žåå¿ã
ããšããŒã«ç¡«é žåå¿ãã«ã«ããŸãŒã«ç¡«é žå
å¿ãéœæ§ã§ãããããŠãŒã¬ããåå¿ããã³
ãããªã³åå¿ãããŒãªãŒããªãŒãªã³åå¿ã
ãšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ãé°æ§
ã§ããã (6) 玫å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã玫å€éš
åžåã¹ãã¯ãã«ã瀺ãã (7) èµ€å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºãèµ€å€éš
åžåã¹ãã¯ãã«ã瀺ãã (8) 13CâNMRã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã13Câ
NMRã¹ãã¯ãã«ã瀺ãã (9) æ§æç³ïŒã°ã«ã³ãŒã¹ã®ã¿ã§æ§æãããã (10) æ§é ïŒÎ±âïŒïŒïŒã°ã«ã³ã·ãçµåãäž»éªšæ Œãš
ããçŽ10åã®Î±âïŒïŒïŒçµåã§çµã°ããã°
ã«ã³ãŒã¹æ®åºã«ïŒåã®å²åã§Î±âïŒïŒïŒçµ
åãæããã (11) èç¹ïŒäžå®ã®èç¹ã瀺ããã220âã§è€å€ãã
280âã§é»å€ããŠçåãèµ·ããã ïŒ ç±³ç³ ãç±æ°Žæœåºããæœåºç©ãããã«é€èçœæ
äœããããšã«ããåŸããã以äžã®æ§è³ªãæããæ°
èŠå€ç³äœRDPç©è³ªãŸãã¯è©²ç©è³ªãšè£œå€äžèš±å®¹ã
ãã賊圢å€ããå®è³ªçã«ãªããã®ãæå¹æåãšã
ãå ç«èª¿ç¯å€ã (1) ååéïŒéæèãééãããååéïŒäžä»¥äž
ãšæšå®ãããã (2) 溶解æ§ïŒã¢ã«ã³ãŒã«ãã¢ã»ãã³ããããµã³ã
ãã³ãŒã³ãé ¢é žãšãã«ããžã¡ãã«ã¹ã«ãã
ã·ãããªã°ãã€ã³ãåå¡©åççŽ ãã¯ããã
ã«ã ããã³ãšãŒãã«ã«äžæº¶ã§ãããæ°Žã«ã¯
å¯æº¶ã§ããã (3) 氎溶液ã®PHïŒïŒïŒ 氎溶液ã¯äžæ§ã瀺ãã (4) èçœè³ªãç¡æ©è³ªïŒèçœè³ªãå«æãããïŒïŒ 以
äžã®ç¡æ©è³ªãå«æããã (5) åè²åå¿ïŒã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«
é žåå¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ã
ã€ã³ç¡«é žåå¿ãã¯ãã¢ãããŒãç¡«é žåå¿ã
ããšããŒã«ç¡«é žåå¿ãã«ã«ããŸãŒã«ç¡«é žå
å¿ãéœæ§ã§ãããããŠãŒã¬ããåå¿ããã³
ãããªã³åå¿ãããŒãªãŒããªãŒãªã³åå¿ã
ãšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ãé°æ§
ã§ããã (6) 玫å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã玫å€éš
åžåã¹ãã¯ãã«ã瀺ãã (7) èµ€å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºãèµ€å€éš
åžåã¹ãã¯ãã«ã瀺ãã (8) 13CâNMRã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã13Câ
NMRã¹ãã¯ãã«ã瀺ãã (9) æ§æç³ïŒã°ã«ã³ãŒã¹ã®ã¿ã§æ§æãããã (10) æ§é ïŒÎ±âïŒïŒïŒã°ã«ã³ã·ãçµåãäž»éªšæ Œãš
ããçŽ10åã®Î±âïŒïŒïŒçµåã§çµã°ããã°
ã«ã³ãŒã¹æ®åºã«ïŒåã®å²åã§Î±âïŒïŒïŒçµ
åãæããã (11) èç¹ïŒäžå®ã®èç¹ã瀺ããã220âã§è€å€ãã
280âã§é»å€ããŠçåãèµ·ããã ïŒ ç±³ç³ ãç±æ°Žæœåºããæœåºç©ãããã«é€èçœæ
äœããããšã«ããåŸããã以äžã®æ§è³ªãæããæ°
èŠå€ç³äœRDPç©è³ªãŸãã¯è©²ç©è³ªãšè£œå€äžèš±å®¹ã
ãã賊圢å€ããå®è³ªçã«ãªããã®ãæå¹æåãšã
ãææçäºé²æ²»çå€ã (1) ååéïŒéæèãééãããååéïŒäžä»¥äž
ãšæšå®ãããã (2) 溶解æ§ïŒã¢ã«ã³ãŒã«ãã¢ã»ãã³ããããµã³ã
ãã³ãŒã³ãé ¢é žãšãã«ããžã¡ãã«ã¹ã«ãã
ã·ãããªã°ãã€ã³ãåå¡©åççŽ ãã¯ããã
ã«ã ããã³ãšãŒãã«ã«äžæº¶ã§ãããæ°Žã«ã¯
å¯æº¶ã§ããã (3) 氎溶液ã®PHïŒïŒïŒ 氎溶液ã¯äžæ§ã瀺ãã (4) èçœè³ªãç¡æ©è³ªïŒèçœè³ªãå«æãããïŒïŒ 以
äžã®ç¡æ©è³ªãå«æããã (5) åè²åå¿ïŒã¢ãŒãªãã·ãŠåå¿ãã¢ã³ã¹ãã³ç¡«
é žåå¿ãããªãããã¢ã³ç¡«é žåå¿ãã·ã¹ã
ã€ã³ç¡«é žåå¿ãã¯ãã¢ãããŒãç¡«é žåå¿ã
ããšããŒã«ç¡«é žåå¿ãã«ã«ããŸãŒã«ç¡«é žå
å¿ãéœæ§ã§ãããããŠãŒã¬ããåå¿ããã³
ãããªã³åå¿ãããŒãªãŒããªãŒãªã³åå¿ã
ãšã«ãœã³ã¢ã«ã¬ã³åå¿ããšãŒãåå¿ãé°æ§
ã§ããã (6) 玫å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã玫å€éš
åžåã¹ãã¯ãã«ã瀺ãã (7) èµ€å€éšåžåã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºãèµ€å€éš
åžåã¹ãã¯ãã«ã瀺ãã (8) 13CâNMRã¹ãã¯ãã«ïŒç¬¬ïŒå³ã«ç€ºã13Câ
NMRã¹ãã¯ãã«ã瀺ãã (9) æ§æç³ïŒã°ã«ã³ãŒã¹ã®ã¿ã§æ§æãããã (10) æ§é ïŒÎ±âïŒïŒïŒã°ã«ã³ã·ãçµåãäž»éªšæ Œãš
ããçŽ10åã®Î±âïŒïŒïŒçµåã§çµã°ããã°
ã«ã³ãŒã¹æ®åºã«ïŒåã®å²åã§Î±âïŒïŒïŒçµ
åãæããã (11) èç¹ïŒäžå®ã®èç¹ã瀺ããã220âã§è€å€ãã
280âã§é»å€ããŠçåãèµ·ããã
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58044316A JPS59170017A (ja) | 1983-03-18 | 1983-03-18 | æ°èŠå€ç³äœïœïœïœç©è³ªïŒãã®è£œé æ³ããã³ãããæå¹æåãšããæè «çå€ãå ç«èª¿ç¯å€ããã³ææçäºé²æ²»çå€ |
| US06/586,073 US4764507A (en) | 1983-03-18 | 1984-03-05 | Polysaccharide RDP substance |
| DE19843409029 DE3409029A1 (de) | 1983-03-18 | 1984-03-13 | Polysaccharid-rdp-substanz, verfahren zu ihrer herstellung und ihre verwendung |
| FR848404223A FR2542745B1 (fr) | 1983-03-18 | 1984-03-19 | Polysaccharide, sa preparation et son application en therapeutique |
| GB08407083A GB2139240B (en) | 1983-03-18 | 1984-03-19 | A polysaccharide rdp substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58044316A JPS59170017A (ja) | 1983-03-18 | 1983-03-18 | æ°èŠå€ç³äœïœïœïœç©è³ªïŒãã®è£œé æ³ããã³ãããæå¹æåãšããæè «çå€ãå ç«èª¿ç¯å€ããã³ææçäºé²æ²»çå€ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59170017A JPS59170017A (ja) | 1984-09-26 |
| JPH0372085B2 true JPH0372085B2 (ja) | 1991-11-15 |
Family
ID=12688076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58044316A Granted JPS59170017A (ja) | 1983-03-18 | 1983-03-18 | æ°èŠå€ç³äœïœïœïœç©è³ªïŒãã®è£œé æ³ããã³ãããæå¹æåãšããæè «çå€ãå ç«èª¿ç¯å€ããã³ææçäºé²æ²»çå€ |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4764507A (ja) |
| JP (1) | JPS59170017A (ja) |
| DE (1) | DE3409029A1 (ja) |
| FR (1) | FR2542745B1 (ja) |
| GB (1) | GB2139240B (ja) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6153220A (ja) * | 1984-08-22 | 1986-03-17 | Sapporo Breweries Ltd | æ°èŠå€ç³äœïœïœïœç©è³ªïŒãã®è£œé æ³ããã³ãããæå¹æåãšããæè «çå€ïŒå ç«èª¿ç¯å€äžŠã³ã«ææçäºé²æ²»çå€ |
| JPS6153221A (ja) * | 1984-08-22 | 1986-03-17 | Sapporo Breweries Ltd | æ°èŠå€ç³äœïœïœïœç©è³ªïŒãã®è£œé æ³ããã³ãããæå¹æåãšããæè «çå€ïŒå ç«èª¿ç¯å€äžŠã³ã«ææçäºé²æ²»çå€ |
| WO2000041541A2 (en) * | 1999-01-12 | 2000-07-20 | Vito-Mannan Polysaccharide L.L.C. | Method of isolating mucilaginous polysaccharides and uses thereof |
| US20040166248A1 (en) * | 2000-12-15 | 2004-08-26 | Sheng-Hsin Hu | Coated activated carbon |
| US6740406B2 (en) | 2000-12-15 | 2004-05-25 | Kimberly-Clark Worldwide, Inc. | Coated activated carbon |
| GB2405755B (en) * | 2003-09-04 | 2006-07-05 | Shaukat Consultants Ltd | Lamps for a road vehicle |
| CN100424098C (zh) * | 2006-10-25 | 2008-10-08 | å京äžç§éå¢è¡ä»œæéå ¬åž | äžç§çµèå¢åå€ç³ç粟å¶å·¥èº |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2900284A (en) * | 1955-09-30 | 1959-08-18 | Oshima Motoyoshi | Process for the saccharification of cellulose-containing material |
| GB1444901A (en) * | 1972-05-02 | 1976-08-04 | Milner Scient Medical Research | Glucose polymers |
| AR208286A1 (es) * | 1973-04-10 | 1976-12-20 | Cpc International Inc | Procedimiento para convertir directamente almidon granular en un hidrolizado de almidon soluble |
| SE432426B (sv) * | 1976-05-12 | 1984-04-02 | Cpc International Inc | Sett att framstella en vattenuppslamning av sterkelse |
| US4182756A (en) * | 1977-11-21 | 1980-01-08 | Abbott Laboratories | High calorie solutions of low molecular weight glucose polymer mixtures useful for intravenous administration |
| JPS5943929B2 (ja) * | 1979-08-13 | 1984-10-25 | ãµãããããŒã«æ ªåŒäŒç€Ÿ | å€ç³äœïœïœïœç©è³ªïŒãã®è£œæ³ããã³ãããæå¹æåãšããæè «çæ§å€ |
-
1983
- 1983-03-18 JP JP58044316A patent/JPS59170017A/ja active Granted
-
1984
- 1984-03-05 US US06/586,073 patent/US4764507A/en not_active Expired - Fee Related
- 1984-03-13 DE DE19843409029 patent/DE3409029A1/de active Granted
- 1984-03-19 FR FR848404223A patent/FR2542745B1/fr not_active Expired
- 1984-03-19 GB GB08407083A patent/GB2139240B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS59170017A (ja) | 1984-09-26 |
| GB2139240A (en) | 1984-11-07 |
| US4764507A (en) | 1988-08-16 |
| FR2542745B1 (fr) | 1989-02-03 |
| GB2139240B (en) | 1986-05-14 |
| DE3409029C2 (ja) | 1989-02-16 |
| DE3409029A1 (de) | 1984-10-11 |
| GB8407083D0 (en) | 1984-04-26 |
| FR2542745A1 (fr) | 1984-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS5943929B2 (ja) | å€ç³äœïœïœïœç©è³ªïŒãã®è£œæ³ããã³ãããæå¹æåãšããæè «çæ§å€ | |
| US4313934A (en) | Physiologically active polysaccharides, production and uses thereof | |
| SU704431A3 (ru) | СпПÑПб пПлÑÑеМО пПлОÑÐ°Ñ Ð°ÑОЎПв, ПблаЎаÑÑÐžÑ Ð¿ÑПÑОвППпÑÑ ÐŸÐ»ÐµÐ²ÑÐŒ ЎейÑÑвОеЌ | |
| JPS627173B2 (ja) | ||
| JPH0372085B2 (ja) | ||
| CN106243239B (zh) | äžç§çšäºæé«èçæ£è å ç«åçå¯æº¶æ§å°ååβ-1,3-è¡èç³ | |
| JPH0539305A (ja) | ã¢ã¹ãã©ã¬ã«ã¹ã»ã¡ã³ãã©ãã»ãŠã¹ããæœåºããå ç« å€èª¿æ§å€ç³é¡åã³ããããå«æããè¬å€çµæç© | |
| JPH06256208A (ja) | å ç«è³ŠæŽ»å€ | |
| US4614733A (en) | Polysaccharides pharmaceutical compositions and the use thereof | |
| JPH07116241B2 (ja) | ç±³ç³ ç±æ¥çç掻æ§å€ç³ïœïœïœã®è£œé æ³ | |
| JPS61257930A (ja) | ææé²åŸ¡å€ | |
| JPH11228602A (ja) | å ç«å匷åå€åã³å ç«å匷åé£å | |
| KR870001931B1 (ko) | ë€ë¹ì²Ž rin 묌ì§ì ì ì¡°ë°©ë² | |
| JPH06247861A (ja) | æ朰çå€ããã³ãã®è£œé æ³ | |
| JPS5936964B2 (ja) | å ç«è³ŠæŽ»å€ | |
| JPS6058925A (ja) | æè «ç掻æ§ç©è³ª | |
| JPS59170018A (ja) | ææçäºé²æ²»çå€ | |
| JPS6338325B2 (ja) | ||
| JPH0748268A (ja) | ã¢ãã©ãŒãŒé»å®³ç©è³ªã®èª¿è£œæ¹æ³ | |
| JPS5931715A (ja) | å¶çæ§ç©è³ªïœïœâïŒïŒïŒã®è£œé æ³ | |
| JPH0748400A (ja) | ã¢ãã©ãŒãŒé»å®³ç©è³ªã®è£œé æ¹æ³ | |
| KR810001499B1 (ko) | ì§ì륌 íšì íë ë€ë¹ë¥ì ì ì¡°ë°©ë² | |
| JPS58121297A (ja) | æ°èŠãªãç³èçœè³ªïœïœïœïŒãã®è£œé æ³ããã³è©²ç³èçœè³ªãæå¹æåãšããŠå«æããå¶çå€ | |
| JPS608226A (ja) | äœååèçœè³ªãæå¹æåãšããå¶ã¬ã³å€ | |
| JPS6352886A (ja) | å€ç³é¡ã®è£œé æ³ |