JP7731555B2 - 多能性幹細胞の分化抑制方法 - Google Patents

多能性幹細胞の分化抑制方法

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Publication number
JP7731555B2
JP7731555B2 JP2022500470A JP2022500470A JP7731555B2 JP 7731555 B2 JP7731555 B2 JP 7731555B2 JP 2022500470 A JP2022500470 A JP 2022500470A JP 2022500470 A JP2022500470 A JP 2022500470A JP 7731555 B2 JP7731555 B2 JP 7731555B2
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Prior art keywords
culture
cells
inhibitor
pluripotent stem
medium
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Japanese (ja)
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JPWO2021162090A1 (https=
Inventor
一博 竹内
将人 伊吹
洋平 林
真美 ▲高▼▲崎▼
昌 神林
義和 河井
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Kaneka Corp
RIKEN
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Kaneka Corp
RIKEN
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022500470A 2020-02-12 2021-02-12 多能性幹細胞の分化抑制方法 Active JP7731555B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020021843 2020-02-12
JP2020021843 2020-02-12
PCT/JP2021/005255 WO2021162090A1 (ja) 2020-02-12 2021-02-12 多能性幹細胞の分化抑制方法

Publications (2)

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JPWO2021162090A1 JPWO2021162090A1 (https=) 2021-08-19
JP7731555B2 true JP7731555B2 (ja) 2025-09-01

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JP2022500470A Active JP7731555B2 (ja) 2020-02-12 2021-02-12 多能性幹細胞の分化抑制方法

Country Status (5)

Country Link
US (1) US20230094568A1 (https=)
EP (1) EP4105319A4 (https=)
JP (1) JP7731555B2 (https=)
CN (1) CN115135755A (https=)
WO (1) WO2021162090A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102448428B1 (ko) 2017-01-27 2022-09-30 가부시키가이샤 가네카 내배엽계 세포 집단, 및 다능성 세포로부터 3배엽 중 어느 하나의 세포 집단을 제조하는 방법
WO2023120420A1 (ja) * 2021-12-20 2023-06-29 株式会社カネカ 多能性幹細胞ストックの大量製造方法
US20250145965A1 (en) * 2022-02-04 2025-05-08 Riken Production method for induced pluripotent stem cells
JP2023114300A (ja) * 2022-02-04 2023-08-17 国立研究開発法人理化学研究所 人工多能性幹細胞の製造方法
EP4479521A4 (en) * 2022-02-15 2026-01-28 Univ Southern California METHODS OF DEVIATION AND PROPAGATION OF AVILY MULTIPOTENTED STEM CELLS AND THEIR APPLICATIONS
CN114591894B (zh) * 2022-02-28 2024-01-09 中国人民解放军总医院 一种皮肤多能前体干细胞的制备方法及其应用
EP4617362A1 (en) 2022-11-09 2025-09-17 Kaneka Corporation Method for producing pluripotent stem cells and method for inducing differentiation of pluripotent stem cells
WO2025206288A1 (ja) * 2024-03-29 2025-10-02 株式会社カネカ 多能性幹細胞の分化効率向上法

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013077423A1 (ja) 2011-11-25 2013-05-30 国立大学法人京都大学 多能性幹細胞の培養方法
US20130236961A1 (en) 2010-09-07 2013-09-12 Technion Research & Development Foundation Limited Novel methods and culture media for culturing pluripotent stem cells
WO2015076388A1 (ja) 2013-11-22 2015-05-28 国立研究開発法人理化学研究所 終脳又はその前駆組織の製造方法
JP2017525351A (ja) 2014-07-30 2017-09-07 イェダ リサーチ アンド ディベロップメント カンパニー リミテッドＹｅｄａ Ｒｅｓｅａｒｃｈ Ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔ Ｃｏ．Ｌｔｄ． 多能性幹細胞の培養用培地
WO2017159862A1 (ja) 2016-03-18 2017-09-21 国立大学法人京都大学 多能性幹細胞由来心筋細胞の凝集体の凍結方法
WO2018097253A1 (ja) 2016-11-25 2018-05-31 国立研究開発法人理化学研究所 移植用細胞集団及びその製造方法
WO2019093340A1 (ja) 2017-11-07 2019-05-16 国立大学法人京都大学 ナイーブ型多能性幹細胞からの原始内胚葉誘導方法
WO2019131940A1 (ja) 2017-12-28 2019-07-04 株式会社カネカ 多能性幹細胞凝集抑制剤
WO2019151386A1 (ja) 2018-01-31 2019-08-08 富士フイルム株式会社 細胞の製造方法

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SG11201701398SA (en) * 2014-08-22 2017-03-30 Cambridge Entpr Ltd Resetting pluripotent stem cells
US20190194621A1 (en) * 2016-09-08 2019-06-27 Kyowa Hakko Bio Co., Ltd. Medium for culturing stem cells, method for culturing stem cells, growth promoter for stem cells, and cell composition and method for producing same
US20190316081A1 (en) * 2018-04-17 2019-10-17 Yonatan Y. Lipsitz Media and methods for expansion of pluripotent stem cells
JP7022340B2 (ja) 2018-08-01 2022-02-18 日本電気硝子株式会社 カメラのキャリブレーション方法、キャリブレーション装置及びキャリブレーション用ターゲット
WO2021020267A1 (ja) * 2019-07-26 2021-02-04 富士フイルム株式会社 ナイーブ型多能性幹細胞の増殖能亢進用培地および多能性幹細胞の製造方法

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US20130236961A1 (en) 2010-09-07 2013-09-12 Technion Research & Development Foundation Limited Novel methods and culture media for culturing pluripotent stem cells
WO2013077423A1 (ja) 2011-11-25 2013-05-30 国立大学法人京都大学 多能性幹細胞の培養方法
WO2015076388A1 (ja) 2013-11-22 2015-05-28 国立研究開発法人理化学研究所 終脳又はその前駆組織の製造方法
JP2017525351A (ja) 2014-07-30 2017-09-07 イェダ リサーチ アンド ディベロップメント カンパニー リミテッドＹｅｄａ Ｒｅｓｅａｒｃｈ Ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔ Ｃｏ．Ｌｔｄ． 多能性幹細胞の培養用培地
WO2017159862A1 (ja) 2016-03-18 2017-09-21 国立大学法人京都大学 多能性幹細胞由来心筋細胞の凝集体の凍結方法
WO2018097253A1 (ja) 2016-11-25 2018-05-31 国立研究開発法人理化学研究所 移植用細胞集団及びその製造方法
WO2019093340A1 (ja) 2017-11-07 2019-05-16 国立大学法人京都大学 ナイーブ型多能性幹細胞からの原始内胚葉誘導方法
WO2019131940A1 (ja) 2017-12-28 2019-07-04 株式会社カネカ 多能性幹細胞凝集抑制剤
WO2019151386A1 (ja) 2018-01-31 2019-08-08 富士フイルム株式会社 細胞の製造方法

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ANGELES,Alejandro De Los，Generation of ERK-Independent Human and Non-Human Primate PLuripotent Stem Cells，Current Protocols in Stem Cell Biology，2019年，vol.49,e85，p.1-17

Also Published As

Publication number Publication date
EP4105319A4 (en) 2024-03-20
WO2021162090A1 (ja) 2021-08-19
JPWO2021162090A1 (https=) 2021-08-19
EP4105319A1 (en) 2022-12-21
US20230094568A1 (en) 2023-03-30
CN115135755A (zh) 2022-09-30

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