CN115135755A - 多能干细胞分化的抑制方法 - Google Patents

多能干细胞分化的抑制方法 Download PDF

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Publication number
CN115135755A
CN115135755A CN202180014472.0A CN202180014472A CN115135755A CN 115135755 A CN115135755 A CN 115135755A CN 202180014472 A CN202180014472 A CN 202180014472A CN 115135755 A CN115135755 A CN 115135755A
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inhibitor
cells
culture
pluripotent stem
less
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CN202180014472.0A
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English (en)
Chinese (zh)
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竹内一博
伊吹将人
林洋平
高崎真美
神林昌
河井义和
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Kaneka Corp
RIKEN
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Kaneka Corp
RIKEN
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202180014472.0A 2020-02-12 2021-02-12 多能干细胞分化的抑制方法 Pending CN115135755A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-021843 2020-02-12
JP2020021843 2020-02-12
PCT/JP2021/005255 WO2021162090A1 (ja) 2020-02-12 2021-02-12 多能性幹細胞の分化抑制方法

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CN115135755A true CN115135755A (zh) 2022-09-30

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US (1) US20230094568A1 (https=)
EP (1) EP4105319A4 (https=)
JP (1) JP7731555B2 (https=)
CN (1) CN115135755A (https=)
WO (1) WO2021162090A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110234754A (zh) * 2017-01-27 2019-09-13 株式会社钟化 内胚层细胞团、和由多能细胞制造三胚层中的任意胚层的细胞团的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023120420A1 (ja) * 2021-12-20 2023-06-29 株式会社カネカ 多能性幹細胞ストックの大量製造方法
US20250145965A1 (en) * 2022-02-04 2025-05-08 Riken Production method for induced pluripotent stem cells
JP2023114300A (ja) * 2022-02-04 2023-08-17 国立研究開発法人理化学研究所 人工多能性幹細胞の製造方法
EP4479521A4 (en) * 2022-02-15 2026-01-28 Univ Southern California METHODS OF DEVIATION AND PROPAGATION OF AVILY MULTIPOTENTED STEM CELLS AND THEIR APPLICATIONS
CN114591894B (zh) * 2022-02-28 2024-01-09 中国人民解放军总医院 一种皮肤多能前体干细胞的制备方法及其应用
EP4617362A1 (en) 2022-11-09 2025-09-17 Kaneka Corporation Method for producing pluripotent stem cells and method for inducing differentiation of pluripotent stem cells
WO2025206288A1 (ja) * 2024-03-29 2025-10-02 株式会社カネカ 多能性幹細胞の分化効率向上法

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US20170275593A1 (en) * 2014-07-30 2017-09-28 Yeda Research And Development Co. Ltd. Media for culturing pluripotent stem cells
WO2019131940A1 (ja) * 2017-12-28 2019-07-04 株式会社カネカ 多能性幹細胞凝集抑制剤
WO2019151386A1 (ja) * 2018-01-31 2019-08-08 富士フイルム株式会社 細胞の製造方法

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JP6283223B2 (ja) 2010-09-07 2018-02-21 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド 多能性幹細胞を培養するための新規な方法および培養培地
JP5999658B2 (ja) * 2011-11-25 2016-09-28 国立大学法人京都大学 多能性幹細胞の培養方法
ES2732730T3 (es) 2013-11-22 2019-11-25 Riken Método para producir telencéfalo o tejido progenitor del mismo
SG11201701398SA (en) * 2014-08-22 2017-03-30 Cambridge Entpr Ltd Resetting pluripotent stem cells
WO2017159862A1 (ja) 2016-03-18 2017-09-21 国立大学法人京都大学 多能性幹細胞由来心筋細胞の凝集体の凍結方法
US20190194621A1 (en) * 2016-09-08 2019-06-27 Kyowa Hakko Bio Co., Ltd. Medium for culturing stem cells, method for culturing stem cells, growth promoter for stem cells, and cell composition and method for producing same
MY199613A (en) 2016-11-25 2023-11-09 Riken Cell population for transplantation and method for producing same
WO2019093340A1 (ja) 2017-11-07 2019-05-16 国立大学法人京都大学 ナイーブ型多能性幹細胞からの原始内胚葉誘導方法
US20190316081A1 (en) * 2018-04-17 2019-10-17 Yonatan Y. Lipsitz Media and methods for expansion of pluripotent stem cells
JP7022340B2 (ja) 2018-08-01 2022-02-18 日本電気硝子株式会社 カメラのキャリブレーション方法、キャリブレーション装置及びキャリブレーション用ターゲット
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WO2019131940A1 (ja) * 2017-12-28 2019-07-04 株式会社カネカ 多能性幹細胞凝集抑制剤
WO2019151386A1 (ja) * 2018-01-31 2019-08-08 富士フイルム株式会社 細胞の製造方法

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110234754A (zh) * 2017-01-27 2019-09-13 株式会社钟化 内胚层细胞团、和由多能细胞制造三胚层中的任意胚层的细胞团的方法
US11746331B2 (en) 2017-01-27 2023-09-05 Kaneka Corporation Endodermal cell population, and method for producing cell population of any of three germ layers from pluripotent cell
CN110234754B (zh) * 2017-01-27 2024-02-20 株式会社钟化 内胚层细胞团、和由多能细胞制造三胚层中的任意胚层的细胞团的方法

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JP7731555B2 (ja) 2025-09-01
EP4105319A4 (en) 2024-03-20
WO2021162090A1 (ja) 2021-08-19
JPWO2021162090A1 (https=) 2021-08-19
EP4105319A1 (en) 2022-12-21
US20230094568A1 (en) 2023-03-30

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