EP4105319A4 - Method for suppressing differentiation of pluripotent stem cells - Google Patents

Method for suppressing differentiation of pluripotent stem cells Download PDF

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Publication number
EP4105319A4
EP4105319A4 EP21754008.7A EP21754008A EP4105319A4 EP 4105319 A4 EP4105319 A4 EP 4105319A4 EP 21754008 A EP21754008 A EP 21754008A EP 4105319 A4 EP4105319 A4 EP 4105319A4
Authority
EP
European Patent Office
Prior art keywords
stem cells
pluripotent stem
suppressing differentiation
differentiation
suppressing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21754008.7A
Other languages
German (de)
French (fr)
Other versions
EP4105319A1 (en
Inventor
Kazuhiro Takeuchi
Masato Ibuki
Yohei Hayashi
Mami TAKASAKI
Sho KAMBAYASHI
Yoshikazu Kawai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaneka Corp
RIKEN Institute of Physical and Chemical Research
Original Assignee
Kaneka Corp
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp, RIKEN Institute of Physical and Chemical Research filed Critical Kaneka Corp
Publication of EP4105319A1 publication Critical patent/EP4105319A1/en
Publication of EP4105319A4 publication Critical patent/EP4105319A4/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP21754008.7A 2020-02-12 2021-02-12 Method for suppressing differentiation of pluripotent stem cells Pending EP4105319A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020021843 2020-02-12
PCT/JP2021/005255 WO2021162090A1 (en) 2020-02-12 2021-02-12 Method for suppressing differentiation of pluripotent stem cells

Publications (2)

Publication Number Publication Date
EP4105319A1 EP4105319A1 (en) 2022-12-21
EP4105319A4 true EP4105319A4 (en) 2024-03-20

Family

ID=77292727

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21754008.7A Pending EP4105319A4 (en) 2020-02-12 2021-02-12 Method for suppressing differentiation of pluripotent stem cells

Country Status (5)

Country Link
US (1) US20230094568A1 (en)
EP (1) EP4105319A4 (en)
JP (1) JPWO2021162090A1 (en)
CN (1) CN115135755A (en)
WO (1) WO2021162090A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11746331B2 (en) 2017-01-27 2023-09-05 Kaneka Corporation Endodermal cell population, and method for producing cell population of any of three germ layers from pluripotent cell
WO2023120420A1 (en) * 2021-12-20 2023-06-29 株式会社カネカ Mass production method of pluripotent stem cell stock
WO2023149495A1 (en) * 2022-02-04 2023-08-10 国立研究開発法人理化学研究所 Production method for induced pluripotent stem cells
CN114591894B (en) * 2022-02-28 2024-01-09 中国人民解放军总医院 Preparation method and application of skin multipotent precursor stem cells
WO2024101385A1 (en) * 2022-11-09 2024-05-16 株式会社カネカ Method for producing pluripotent stem cells and method for inducing differentiation of pluripotent stem cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170275593A1 (en) * 2014-07-30 2017-09-28 Yeda Research And Development Co. Ltd. Media for culturing pluripotent stem cells
WO2019131940A1 (en) * 2017-12-28 2019-07-04 株式会社カネカ Pluripotent stem cell aggregation inhibitor

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2746482T3 (en) * 2010-09-07 2020-03-06 Technion Res & Dev Foundation New methods and culture media to cultivate pluripotent stem cells
JP5999658B2 (en) * 2011-11-25 2016-09-28 国立大学法人京都大学 Method for culturing pluripotent stem cells
CN111684063A (en) * 2018-01-31 2020-09-18 富士胶片株式会社 Method for producing cell
JP7022340B2 (en) 2018-08-01 2022-02-18 日本電気硝子株式会社 Camera calibration method, calibration device and calibration target
WO2021020267A1 (en) * 2019-07-26 2021-02-04 富士フイルム株式会社 Medium for enhancing proliferation ability of naive type pluripotent stem cells and method for producing pluripotent stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170275593A1 (en) * 2014-07-30 2017-09-28 Yeda Research And Development Co. Ltd. Media for culturing pluripotent stem cells
WO2019131940A1 (en) * 2017-12-28 2019-07-04 株式会社カネカ Pluripotent stem cell aggregation inhibitor
US20220267736A1 (en) * 2017-12-28 2022-08-25 Kaneka Corporation Pluripotent stem cell aggregation suppressor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GE GUO ET AL: "Epigenetic resetting of human pluripotency", DEVELOPMENT, vol. 144, no. 15, 31 August 2017 (2017-08-31), GB, pages 2748 - 2763, XP055456607, ISSN: 0950-1991, DOI: 10.1242/dev.146811 *
See also references of WO2021162090A1 *

Also Published As

Publication number Publication date
EP4105319A1 (en) 2022-12-21
US20230094568A1 (en) 2023-03-30
JPWO2021162090A1 (en) 2021-08-19
WO2021162090A1 (en) 2021-08-19
CN115135755A (en) 2022-09-30

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Ipc: C12N 5/074 20100101ALI20240215BHEP

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