JP7677950B2 - ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法 - Google Patents

ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法 Download PDF

Info

Publication number
JP7677950B2
JP7677950B2 JP2022513303A JP2022513303A JP7677950B2 JP 7677950 B2 JP7677950 B2 JP 7677950B2 JP 2022513303 A JP2022513303 A JP 2022513303A JP 2022513303 A JP2022513303 A JP 2022513303A JP 7677950 B2 JP7677950 B2 JP 7677950B2
Authority
JP
Japan
Prior art keywords
nucleic acid
hbv
seq
primers
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022513303A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022546443A (ja
JP2022546443A5 (enExample
Inventor
フォン ジェフリー
サディアス ハミルトン アーロン
ヘイル マリンサ
コズロフ イゴル
グスタボ マリンズ エド
マリー スコット エリザベス
ワン リン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2022546443A publication Critical patent/JP2022546443A/ja
Publication of JP2022546443A5 publication Critical patent/JP2022546443A5/ja
Application granted granted Critical
Publication of JP7677950B2 publication Critical patent/JP7677950B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ecology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022513303A 2019-08-27 2020-02-24 ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法 Active JP7677950B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962892132P 2019-08-27 2019-08-27
US62/892,132 2019-08-27
PCT/EP2020/054715 WO2021037399A1 (en) 2019-08-27 2020-02-24 Compositions and methods for amplification and detection of hepatitis b virus rna, including hbv rna transcribed from cccdna

Publications (3)

Publication Number Publication Date
JP2022546443A JP2022546443A (ja) 2022-11-04
JP2022546443A5 JP2022546443A5 (enExample) 2023-03-03
JP7677950B2 true JP7677950B2 (ja) 2025-05-15

Family

ID=69701185

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022513303A Active JP7677950B2 (ja) 2019-08-27 2020-02-24 ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法

Country Status (6)

Country Link
US (1) US11739382B2 (enExample)
EP (1) EP4022086B1 (enExample)
JP (1) JP7677950B2 (enExample)
CN (1) CN114286866A (enExample)
ES (1) ES3039650T3 (enExample)
WO (1) WO2021037399A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4022086B1 (en) 2019-08-27 2025-08-06 F. Hoffmann-La Roche AG Compositions and methods for amplification and detection of hepatitis b virus rna, including hbv rna transcribed from cccdna
US20220275465A1 (en) * 2019-08-27 2022-09-01 Roche Molecular Systems, Inc. COMPOSITIONS AND METHODS FOR AMPLIFICATION AND DETECTION OF HEPATITIS B VIRUS RNA, INCLUDING HBV RNA TRANSCRIBED FROM cccDNA
CN115125334A (zh) * 2022-06-29 2022-09-30 广州达安基因股份有限公司 一种乙型肝炎病毒rna检测试剂盒及方法
CN119452103A (zh) * 2022-09-02 2025-02-14 学校法人北里研究所 评估幽门螺旋杆菌除菌后的患者罹患胃癌的风险的方法、多核苷酸和试剂盒
CN117512209A (zh) * 2023-09-20 2024-02-06 南方医科大学南方医院 一种检测hbv剪接型rna的引物和探针组合物及其应用
CN119433101A (zh) * 2024-11-28 2025-02-14 郑州安图生物工程股份有限公司 用于定量扩增和检测血清或血浆中hbv rna的引物组和方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090220969A1 (en) 2007-09-28 2009-09-03 North Carolina State University Identifying and quantifying small RNAs
JP2015530113A (ja) 2012-09-28 2015-10-15 セファイド マイクロｒｎａ多重アッセイのための２プライマーｐｃｒ
JP2017501683A (ja) 2013-11-11 2017-01-19 パナジーン・インコーポレイテッド クランピングプローブ及び検出プローブを用いた多重標的核酸の検出方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0135587B2 (en) 1983-02-22 2002-12-18 Syngene, Inc. Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP1067134B1 (en) 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
JP3451667B2 (ja) 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
ES2253778T3 (es) 1996-06-04 2006-06-01 University Of Utah Research Foundation Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia.
EP0866071B1 (en) 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
US20040058314A1 (en) 2002-05-29 2004-03-25 Ming Liang He Assay for the detection and quantification of HBV cccDNA by real-time PCR
JP4386660B2 (ja) 2003-03-25 2009-12-16 株式会社先端生命科学研究所 Ｈｂｖ−ｒｎａを含むｈｂｖ粒子
US20120045747A1 (en) 2010-08-23 2012-02-23 Samsung Techwin Co., Ltd. Kit for detecting hepatitis b virus and method for detecting hepatitis b virus using the same
EP1783211A4 (en) 2004-06-22 2007-08-08 Advanced Life Science Inst Inc HBV PARTICLE WITH HBV RNA
ES2371088T3 (es) 2005-10-05 2011-12-27 F. Hoffmann-La Roche Ag Transferencia de energía no fluorescente.
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
KR101822609B1 (ko) * 2010-06-24 2018-01-26 주식회사 파나진 Ｐｎａ 기반의 실시간 ｐｃｒ 클램핑을 이용한 마이크로ｒｎａ 발현양상의 분석 방법 및 키트
US9175332B2 (en) * 2010-07-29 2015-11-03 Roche Molecular Systems, Inc. Generic PCR
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
CN104884636B (zh) 2012-10-30 2018-09-18 新加坡科技研究局 Hbv对肝细胞癌癌症患者临床结果的影响
CN103898100B (zh) * 2012-12-26 2017-09-22 深圳先进技术研究院 cccDNA标准品及其制备方法、定量检测乙肝病毒cccDNA的方法及试剂盒
EP3052644B1 (en) 2013-10-01 2019-07-10 Texcell Detection of rare microbiological nucleic acids
CN104372106A (zh) * 2014-09-05 2015-02-25 徐州市传染病医院 一种LAMP法检测石蜡包埋肝组织中HBV cccDNA的引物和试剂盒
CN104388598B (zh) 2014-12-11 2016-08-24 上海五色石医学研究有限公司 一种HBV cccDNA数字PCR定量检测试剂盒及其应用
US11248255B2 (en) * 2015-06-19 2022-02-15 The Governing Council Of The University Of Toronto Amplification of nanoparticle based assay
CN108603226A (zh) * 2016-02-15 2018-09-28 Jbs科学公司 乙型肝炎病毒cccdna的定量测量
EA202091646A1 (ru) * 2018-01-05 2020-11-17 Квошент Сюисс Са Платформа на основе самособирающегося диагностического массива
EP4022086B1 (en) 2019-08-27 2025-08-06 F. Hoffmann-La Roche AG Compositions and methods for amplification and detection of hepatitis b virus rna, including hbv rna transcribed from cccdna

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090220969A1 (en) 2007-09-28 2009-09-03 North Carolina State University Identifying and quantifying small RNAs
JP2015530113A (ja) 2012-09-28 2015-10-15 セファイド マイクロｒｎａ多重アッセイのための２プライマーｐｃｒ
JP2017501683A (ja) 2013-11-11 2017-01-19 パナジーン・インコーポレイテッド クランピングプローブ及び検出プローブを用いた多重標的核酸の検出方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Arch. Virol.，2019年08月19日，Vol. 164，p. 2683-2690
J. Hepatol.，2018年，Vol. 68，p. S489，FRI-287

Also Published As

Publication number Publication date
EP4022086C0 (en) 2025-08-06
CN114286866A (zh) 2022-04-05
JP2022546443A (ja) 2022-11-04
ES3039650T3 (en) 2025-10-23
WO2021037399A1 (en) 2021-03-04
US20210062259A1 (en) 2021-03-04
US11739382B2 (en) 2023-08-29
EP4022086A1 (en) 2022-07-06
EP4022086B1 (en) 2025-08-06

Similar Documents

Publication Publication Date Title
JP7677950B2 (ja) ｃｃｃＤＮＡから転写されたＨＢＶ ＲＮＡを含む、Ｂ型肝炎ウイルスＲＮＡの増幅および検出のための組成物および方法
JP6911190B2 (ja) バベシア（Ｂａｂｅｓｉａ）の検出のための組成物及び方法
JP7478734B2 (ja) カンジダ・アウリス（ｃａｎｄｉｄａ ａｕｒｉｓ）の検出のための組成物および方法
US10793923B2 (en) Compositions and methods for detection of BK virus
US20220275465A1 (en) COMPOSITIONS AND METHODS FOR AMPLIFICATION AND DETECTION OF HEPATITIS B VIRUS RNA, INCLUDING HBV RNA TRANSCRIBED FROM cccDNA
JP6741665B2 (ja) 重なり合う加水分解プローブを用いた一ヌクレオチド多型検出法
US20240240273A1 (en) Compositions and Methods for Detecting Hepatitis Delta Virus by a Dual-Target Assay
US20240102110A1 (en) Compositions and methods for detection of malaria
JP6999645B2 (ja) 核酸の増幅及び検出／定量の効率を改良するためのヘルパーオリゴヌクレオチド
US10370731B2 (en) Compositions and methods for detection of hepatitis C virus genotype 3
JP7628085B2 (ja) 鎖分離温度に基づくＤＮＡに対してＲＮＡ標的の優先的／選択的増幅のためのｄＩＴＰの利用
JP2026502046A (ja) 複数のｂ型肝炎ウイルス遺伝子標的のためのデジタルｐｃｒアッセイ設計およびそのための非伸長性ブロッカーオリゴヌクレオチド
CN117441029A (zh) 用于检测具有刺突蛋白突变的严重急性呼吸综合征冠状病毒2(sars-cov-2)变体的组合物和方法
CA2994606C (en) Compositions and methods for detection of mycobacterium tuberculosis
CN118202069A (zh) 用于检测疟疾的组合物和方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230222

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230222

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240402

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240702

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20241105

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250204

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250415

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250501

R150 Certificate of patent or registration of utility model

Ref document number: 7677950

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150