JP7460540B2 - 修飾ステロールアシルトランスフェラーゼ - Google Patents

修飾ステロールアシルトランスフェラーゼ Download PDF

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Publication number
JP7460540B2
JP7460540B2 JP2020557258A JP2020557258A JP7460540B2 JP 7460540 B2 JP7460540 B2 JP 7460540B2 JP 2020557258 A JP2020557258 A JP 2020557258A JP 2020557258 A JP2020557258 A JP 2020557258A JP 7460540 B2 JP7460540 B2 JP 7460540B2
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Prior art keywords
dhc
sterol
yeast
are1
modified
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JP2021523692A (ja
JP2021523692A5 (https=
Inventor
クリストファー， マーク ファレル，
リサ， アン ラプラード，
オットー， マーティン レーマン，
ジョシュア トゥルーハート，
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DSM IP Assets BV
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01072—DELTA24-sterol reductase (1.3.1.72)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01026—Sterol O-acyltransferase (2.3.1.26)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020557258A 2018-05-22 2019-05-21 修飾ステロールアシルトランスフェラーゼ Active JP7460540B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH00627/18 2018-05-22
CH6272018 2018-05-22
PCT/EP2019/063076 WO2019224187A1 (en) 2018-05-22 2019-05-21 Modified sterol acyltransferases

Publications (3)

Publication Number Publication Date
JP2021523692A JP2021523692A (ja) 2021-09-09
JP2021523692A5 JP2021523692A5 (https=) 2022-03-09
JP7460540B2 true JP7460540B2 (ja) 2024-04-02

Family

ID=67003439

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020557258A Active JP7460540B2 (ja) 2018-05-22 2019-05-21 修飾ステロールアシルトランスフェラーゼ

Country Status (7)

Country Link
US (1) US12227772B2 (https=)
EP (1) EP3797159A1 (https=)
JP (1) JP7460540B2 (https=)
CN (1) CN112752841B (https=)
BR (1) BR112020023394A2 (https=)
EA (1) EA202092799A1 (https=)
WO (1) WO2019224187A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114606147B (zh) * 2022-03-15 2022-12-16 江南大学 一种同时增强和抑制酿酒酵母7-脱氢胆固醇合成中多个关键基因的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003508052A (ja) 1999-08-30 2003-03-04 モンサント テクノロジー エルエルシー 植物ステロールアシルトランスフェラーゼ
JP2007516717A (ja) 2003-12-24 2007-06-28 ダニスコ エイ／エス タンパク質
JP2010534479A (ja) 2007-07-26 2010-11-11 アムジエン・インコーポレーテツド 変性レシチン−コレステロールアシルトランスフェラーゼ酵素
JP2012024084A (ja) 2010-06-25 2012-02-09 Mitsubishi Chemicals Corp 新規酵母およびそれを用いたδ５，７−ステロール及びハイドロコルチゾンの製造方法
JP2013502903A (ja) 2009-08-26 2013-01-31 オーガノバランス ゲーエムベーハー 脂質の生産のための遺伝的に改変された生物
WO2017108799A1 (en) 2015-12-23 2017-06-29 Dsm Ip Assets B.V. Production of sterols in modified yeast

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10203352A1 (de) 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
ES2607891T3 (es) 2004-07-16 2017-04-04 Dsm Ip Assets B.V. Ingeniería metabólica de células eucariotas que fermentan xilosa
US9624521B2 (en) 2009-12-03 2017-04-18 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
AU2012346662B2 (en) 2011-11-30 2016-07-28 Danisco Us Inc. Yeast strains engineered to produce ethanol from acetic acid and glycerol

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003508052A (ja) 1999-08-30 2003-03-04 モンサント テクノロジー エルエルシー 植物ステロールアシルトランスフェラーゼ
JP2007516717A (ja) 2003-12-24 2007-06-28 ダニスコ エイ／エス タンパク質
JP2010534479A (ja) 2007-07-26 2010-11-11 アムジエン・インコーポレーテツド 変性レシチン−コレステロールアシルトランスフェラーゼ酵素
JP2013502903A (ja) 2009-08-26 2013-01-31 オーガノバランス ゲーエムベーハー 脂質の生産のための遺伝的に改変された生物
JP2012024084A (ja) 2010-06-25 2012-02-09 Mitsubishi Chemicals Corp 新規酵母およびそれを用いたδ５，７−ステロール及びハイドロコルチゾンの製造方法
WO2017108799A1 (en) 2015-12-23 2017-06-29 Dsm Ip Assets B.V. Production of sterols in modified yeast

Also Published As

Publication number Publication date
EP3797159A1 (en) 2021-03-31
US20210115412A1 (en) 2021-04-22
WO2019224187A1 (en) 2019-11-28
JP2021523692A (ja) 2021-09-09
CN112752841B (zh) 2024-04-05
EA202092799A1 (ru) 2021-03-01
CN112752841A (zh) 2021-05-04
US12227772B2 (en) 2025-02-18
BR112020023394A2 (pt) 2021-02-09

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