CN112752841B - 经修饰的甾醇酰基转移酶 - Google Patents

经修饰的甾醇酰基转移酶 Download PDF

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Publication number
CN112752841B
CN112752841B CN201980033817.XA CN201980033817A CN112752841B CN 112752841 B CN112752841 B CN 112752841B CN 201980033817 A CN201980033817 A CN 201980033817A CN 112752841 B CN112752841 B CN 112752841B
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sterol
dhc
leu
yeast
phe
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CN112752841A (zh
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克里斯托弗·马克·法雷尔
丽莎·安·拉普拉德
奥托·马丁·莱曼
约书亚·特鲁哈特
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01072—DELTA24-sterol reductase (1.3.1.72)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01026—Sterol O-acyltransferase (2.3.1.26)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201980033817.XA 2018-05-22 2019-05-21 经修饰的甾醇酰基转移酶 Active CN112752841B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH00627/18 2018-05-22
CH6272018 2018-05-22
PCT/EP2019/063076 WO2019224187A1 (en) 2018-05-22 2019-05-21 Modified sterol acyltransferases

Publications (2)

Publication Number Publication Date
CN112752841A CN112752841A (zh) 2021-05-04
CN112752841B true CN112752841B (zh) 2024-04-05

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CN201980033817.XA Active CN112752841B (zh) 2018-05-22 2019-05-21 经修饰的甾醇酰基转移酶

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US (1) US12227772B2 (https=)
EP (1) EP3797159A1 (https=)
JP (1) JP7460540B2 (https=)
CN (1) CN112752841B (https=)
BR (1) BR112020023394A2 (https=)
EA (1) EA202092799A1 (https=)
WO (1) WO2019224187A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114606147B (zh) * 2022-03-15 2022-12-16 江南大学 一种同时增强和抑制酿酒酵母7-脱氢胆固醇合成中多个关键基因的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101052706A (zh) * 2004-07-16 2007-10-10 代尔夫特技术大学 发酵木糖的真核细胞的代谢工程
CA2773030A1 (en) * 2009-08-26 2011-03-03 Organobalance Gmbh Genetically modified organisms for the production of lipids
WO2011067144A1 (en) * 2009-12-03 2011-06-09 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
WO2017108799A1 (en) * 2015-12-23 2017-06-29 Dsm Ip Assets B.V. Production of sterols in modified yeast
CN107189954A (zh) * 2011-11-30 2017-09-22 帝斯曼知识产权资产管理有限公司 由乙酸和甘油生产乙醇的工程化酵母菌株

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100854607B1 (ko) 1999-08-30 2008-08-27 몬산토 테크놀로지 엘엘씨 식물 스테롤 아실전달효소
DE10203352A1 (de) 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
BRPI0417533B1 (pt) 2003-12-24 2016-03-01 Danisco enzima variante glicolipídio aciltransferase, uso e método para sua produção, método para o preparo de um produto alimentício e produto assado de farinha, processo de refinamento enzimático
HRP20140315T1 (hr) 2007-07-26 2014-05-09 Amgen Inc. Modificirani enzimi lecitin-kolesterol aciltransferaze
JP2012024084A (ja) 2010-06-25 2012-02-09 Mitsubishi Chemicals Corp 新規酵母およびそれを用いたδ５，７−ステロール及びハイドロコルチゾンの製造方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101052706A (zh) * 2004-07-16 2007-10-10 代尔夫特技术大学 发酵木糖的真核细胞的代谢工程
CA2773030A1 (en) * 2009-08-26 2011-03-03 Organobalance Gmbh Genetically modified organisms for the production of lipids
CN102812124A (zh) * 2009-08-26 2012-12-05 器官平衡有限责任公司 用于产生脂质的经遗传改造的生物
WO2011067144A1 (en) * 2009-12-03 2011-06-09 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
CN107189954A (zh) * 2011-11-30 2017-09-22 帝斯曼知识产权资产管理有限公司 由乙酸和甘油生产乙醇的工程化酵母菌株
WO2017108799A1 (en) * 2015-12-23 2017-06-29 Dsm Ip Assets B.V. Production of sterols in modified yeast

Also Published As

Publication number Publication date
EP3797159A1 (en) 2021-03-31
US20210115412A1 (en) 2021-04-22
WO2019224187A1 (en) 2019-11-28
JP7460540B2 (ja) 2024-04-02
JP2021523692A (ja) 2021-09-09
EA202092799A1 (ru) 2021-03-01
CN112752841A (zh) 2021-05-04
US12227772B2 (en) 2025-02-18
BR112020023394A2 (pt) 2021-02-09

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