BR112020023394A2 - esterol aciltransferases modificadas - Google Patents

esterol aciltransferases modificadas Download PDF

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Publication number
BR112020023394A2
BR112020023394A2 BR112020023394-3A BR112020023394A BR112020023394A2 BR 112020023394 A2 BR112020023394 A2 BR 112020023394A2 BR 112020023394 A BR112020023394 A BR 112020023394A BR 112020023394 A2 BR112020023394 A2 BR 112020023394A2
Authority
BR
Brazil
Prior art keywords
dhc
host cell
zimosterol
sterol
enzyme
Prior art date
Application number
BR112020023394-3A
Other languages
English (en)
Portuguese (pt)
Inventor
Christopher Mark Farrell
Lisa Ann Laprade
Otto Martin Lehmann
Joshua Trueheart
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of BR112020023394A2 publication Critical patent/BR112020023394A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01072—DELTA24-sterol reductase (1.3.1.72)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01026—Sterol O-acyltransferase (2.3.1.26)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
BR112020023394-3A 2018-05-22 2019-05-21 esterol aciltransferases modificadas BR112020023394A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH00627/18 2018-05-22
CH6272018 2018-05-22
PCT/EP2019/063076 WO2019224187A1 (en) 2018-05-22 2019-05-21 Modified sterol acyltransferases

Publications (1)

Publication Number Publication Date
BR112020023394A2 true BR112020023394A2 (pt) 2021-02-09

Family

ID=67003439

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112020023394-3A BR112020023394A2 (pt) 2018-05-22 2019-05-21 esterol aciltransferases modificadas

Country Status (7)

Country Link
US (1) US12227772B2 (https=)
EP (1) EP3797159A1 (https=)
JP (1) JP7460540B2 (https=)
CN (1) CN112752841B (https=)
BR (1) BR112020023394A2 (https=)
EA (1) EA202092799A1 (https=)
WO (1) WO2019224187A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114606147B (zh) * 2022-03-15 2022-12-16 江南大学 一种同时增强和抑制酿酒酵母7-脱氢胆固醇合成中多个关键基因的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100854607B1 (ko) 1999-08-30 2008-08-27 몬산토 테크놀로지 엘엘씨 식물 스테롤 아실전달효소
DE10203352A1 (de) 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
BRPI0417533B1 (pt) 2003-12-24 2016-03-01 Danisco enzima variante glicolipídio aciltransferase, uso e método para sua produção, método para o preparo de um produto alimentício e produto assado de farinha, processo de refinamento enzimático
ES2607891T3 (es) 2004-07-16 2017-04-04 Dsm Ip Assets B.V. Ingeniería metabólica de células eucariotas que fermentan xilosa
HRP20140315T1 (hr) 2007-07-26 2014-05-09 Amgen Inc. Modificirani enzimi lecitin-kolesterol aciltransferaze
EP2292741A1 (en) 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids
US9624521B2 (en) 2009-12-03 2017-04-18 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
JP2012024084A (ja) 2010-06-25 2012-02-09 Mitsubishi Chemicals Corp 新規酵母およびそれを用いたδ５，７−ステロール及びハイドロコルチゾンの製造方法
AU2012346662B2 (en) 2011-11-30 2016-07-28 Danisco Us Inc. Yeast strains engineered to produce ethanol from acetic acid and glycerol
CN109154015B (zh) 2015-12-23 2022-03-15 帝斯曼知识产权资产管理有限公司 在经修饰的酵母中生产甾醇

Also Published As

Publication number Publication date
EP3797159A1 (en) 2021-03-31
US20210115412A1 (en) 2021-04-22
WO2019224187A1 (en) 2019-11-28
JP7460540B2 (ja) 2024-04-02
JP2021523692A (ja) 2021-09-09
CN112752841B (zh) 2024-04-05
EA202092799A1 (ru) 2021-03-01
CN112752841A (zh) 2021-05-04
US12227772B2 (en) 2025-02-18

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B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]