EA202092799A1 - Модифицированные стерол-ацилтрансферазы - Google Patents

Модифицированные стерол-ацилтрансферазы

Info

Publication number
EA202092799A1
EA202092799A1 EA202092799A EA202092799A EA202092799A1 EA 202092799 A1 EA202092799 A1 EA 202092799A1 EA 202092799 A EA202092799 A EA 202092799A EA 202092799 A EA202092799 A EA 202092799A EA 202092799 A1 EA202092799 A1 EA 202092799A1
Authority
EA
Eurasian Patent Office
Prior art keywords
vitamin
sterol acyltransferase
modified
modified sterol
relates
Prior art date
Application number
EA202092799A
Other languages
English (en)
Inventor
Кристофер Марк Фаррел
Лиза Энн Лапраде
Отто Мартин Леман
Джошуа Трухарт
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA202092799A1 publication Critical patent/EA202092799A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01072—DELTA24-sterol reductase (1.3.1.72)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01026—Sterol O-acyltransferase (2.3.1.26)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Настоящее изобретение касается модифицированных ферментов - стерол-ацилтрансфераз с улучшенной активностью и/или специфичностью в отношении ацилирования предшественника витамина D3, 7-дегидрохолестерина (7-DHC), для применения при биотехнологическом получении витамина D3. Кроме того, изобретение касается дрожжевых штаммов, экспрессирующих данные модифицированные ферменты, и их применения в способе получения витамина D3 или его производных и/или метаболитов.
EA202092799A 2018-05-22 2019-05-21 Модифицированные стерол-ацилтрансферазы EA202092799A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH6272018 2018-05-22
PCT/EP2019/063076 WO2019224187A1 (en) 2018-05-22 2019-05-21 Modified sterol acyltransferases

Publications (1)

Publication Number Publication Date
EA202092799A1 true EA202092799A1 (ru) 2021-03-01

Family

ID=67003439

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202092799A EA202092799A1 (ru) 2018-05-22 2019-05-21 Модифицированные стерол-ацилтрансферазы

Country Status (7)

Country Link
US (1) US20210115412A1 (ru)
EP (1) EP3797159A1 (ru)
JP (1) JP7460540B2 (ru)
CN (1) CN112752841B (ru)
BR (1) BR112020023394A2 (ru)
EA (1) EA202092799A1 (ru)
WO (1) WO2019224187A1 (ru)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114606147B (zh) * 2022-03-15 2022-12-16 江南大学 一种同时增强和抑制酿酒酵母7-脱氢胆固醇合成中多个关键基因的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0014154B1 (pt) 1999-08-30 2017-03-28 Monsanto Technology Llc sequência e construto de ácido nucleíco, bem como métodos para produzir polipeptídeo semelhante à lecitina: colesterol aciltransferases, para modificar o teor de esteróis de célula de planta e para alterar a produção de óleo por célula de planta
DE10203352A1 (de) 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
BRPI0417533B1 (pt) 2003-12-24 2016-03-01 Danisco enzima variante glicolipídio aciltransferase, uso e método para sua produção, método para o preparo de um produto alimentício e produto assado de farinha, processo de refinamento enzimático
CN101914462B (zh) * 2004-07-16 2013-04-24 Dsm知识产权资产有限公司 发酵木糖的真核细胞的代谢工程
US8168416B2 (en) 2007-07-26 2012-05-01 Amgen Inc. Modified lecithin-cholesterol acyltransferase enzymes
EP2292741A1 (en) 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids
WO2011067144A1 (en) * 2009-12-03 2011-06-09 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
JP2012024084A (ja) 2010-06-25 2012-02-09 Mitsubishi Chemicals Corp 新規酵母およびそれを用いたδ５，７−ステロール及びハイドロコルチゾンの製造方法
EP3321368A3 (en) * 2011-11-30 2018-05-23 DSM IP Assets B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
CN109154015B (zh) * 2015-12-23 2022-03-15 帝斯曼知识产权资产管理有限公司 在经修饰的酵母中生产甾醇

Also Published As

Publication number Publication date
EP3797159A1 (en) 2021-03-31
US20210115412A1 (en) 2021-04-22
CN112752841A (zh) 2021-05-04
BR112020023394A2 (pt) 2021-02-09
JP7460540B2 (ja) 2024-04-02
JP2021523692A (ja) 2021-09-09
WO2019224187A1 (en) 2019-11-28
CN112752841B (zh) 2024-04-05

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