EA202092792A1 - Модифицированные стерол-ацилтрансферазы - Google Patents

Модифицированные стерол-ацилтрансферазы

Info

Publication number
EA202092792A1
EA202092792A1 EA202092792A EA202092792A EA202092792A1 EA 202092792 A1 EA202092792 A1 EA 202092792A1 EA 202092792 A EA202092792 A EA 202092792A EA 202092792 A EA202092792 A EA 202092792A EA 202092792 A1 EA202092792 A1 EA 202092792A1
Authority
EA
Eurasian Patent Office
Prior art keywords
vitamin
sterol acyltransferase
modified
modified sterol
relates
Prior art date
Application number
EA202092792A
Other languages
English (en)
Inventor
Отто Мартин Леманн
Гаральд Пихлер
Холли Штольтерфот
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA202092792A1 publication Critical patent/EA202092792A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01072—DELTA24-sterol reductase (1.3.1.72)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01026—Sterol O-acyltransferase (2.3.1.26)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение касается модифицированных ферментов - стерол-ацилтрансфераз с улучшенной активностью и/или специфичностью в отношении ацилирования предшественника витамина D3, 7-дегидрохолестерина (7-DHC) для применения при биотехнологическом производстве витамина D3. Изобретение также касается штаммов хозяина, экспрессирующих данные модифицированные ферменты, и их применения в способе получения витамина D3 или его производных и/или метаболитов.
EA202092792A 2018-05-22 2019-05-21 Модифицированные стерол-ацилтрансферазы EA202092792A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH6282018 2018-05-22
PCT/EP2019/063078 WO2019224188A1 (en) 2018-05-22 2019-05-21 Modified sterol acyltransferases

Publications (1)

Publication Number Publication Date
EA202092792A1 true EA202092792A1 (ru) 2021-04-02

Family

ID=67003440

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202092792A EA202092792A1 (ru) 2018-05-22 2019-05-21 Модифицированные стерол-ацилтрансферазы

Country Status (7)

Country Link
US (1) US20210095325A1 (ru)
EP (1) EP3797158A1 (ru)
JP (1) JP2021524733A (ru)
CN (1) CN112154205A (ru)
BR (1) BR112020023803A2 (ru)
EA (1) EA202092792A1 (ru)
WO (1) WO2019224188A1 (ru)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10203352A1 (de) * 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
WO2011067144A1 (en) 2009-12-03 2011-06-09 Dsm Ip Assets B.V. Production of non-yeast sterols by yeast
US11466303B2 (en) 2015-12-23 2022-10-11 Dsm Ip Assets B.V. Production of sterols in modified yeast

Also Published As

Publication number Publication date
US20210095325A1 (en) 2021-04-01
CN112154205A (zh) 2020-12-29
EP3797158A1 (en) 2021-03-31
JP2021524733A (ja) 2021-09-16
WO2019224188A1 (en) 2019-11-28
BR112020023803A2 (pt) 2021-02-23

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