JP7259182B2 - in situ分子検出のためのハイブリダイゼーション連鎖反応法 - Google Patents
in situ分子検出のためのハイブリダイゼーション連鎖反応法 Download PDFInfo
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- C12Q1/6841—In situ hybridisation
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662326959P | 2016-04-25 | 2016-04-25 | |
| US62/326,959 | 2016-04-25 | ||
| PCT/US2017/029333 WO2017189525A1 (en) | 2016-04-25 | 2017-04-25 | Hybridization chain reaction methods for in situ molecular detection |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019518434A JP2019518434A (ja) | 2019-07-04 |
| JP2019518434A5 JP2019518434A5 (https=) | 2020-06-18 |
| JP7259182B2 true JP7259182B2 (ja) | 2023-04-18 |
Family
ID=60160064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018555974A Active JP7259182B2 (ja) | 2016-04-25 | 2017-04-25 | in situ分子検出のためのハイブリダイゼーション連鎖反応法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US11718874B2 (https=) |
| EP (2) | EP4613756A3 (https=) |
| JP (1) | JP7259182B2 (https=) |
| CN (2) | CN109415761B (https=) |
| AU (2) | AU2017257624B2 (https=) |
| CA (2) | CA3210120C (https=) |
| WO (1) | WO2017189525A1 (https=) |
Families Citing this family (121)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013055995A2 (en) | 2011-10-14 | 2013-04-18 | President And Fellows Of Harvard College | Sequencing by structure assembly |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP3578666A1 (en) | 2013-03-12 | 2019-12-11 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| CN112029826B (zh) | 2014-07-30 | 2025-03-14 | 哈佛学院院长及董事 | 用于测定核酸的系统和方法 |
| US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| JP6882282B2 (ja) | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 三次元核酸含有マトリックスの立体撮像のための方法と装置 |
| CN109415761B (zh) | 2016-04-25 | 2022-09-20 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
| EP4050112A1 (en) | 2016-06-21 | 2022-08-31 | 10X Genomics, Inc. | Nucleic acid sequencing |
| IL324487A (en) | 2016-07-05 | 2026-01-01 | California Inst Of Techn | Hybridization chain reaction based on a minimal initiator |
| CN109923216B (zh) | 2016-08-31 | 2024-08-02 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
| CA3078158A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | Rna templated ligation |
| US20210115504A1 (en) * | 2017-12-08 | 2021-04-22 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridization of probes |
| US12258614B2 (en) | 2018-01-26 | 2025-03-25 | Genans Biotechnology Co., Ltd. | Hybridization chain reaction-based method for amplifying immunosignals |
| CN110441525A (zh) * | 2018-05-02 | 2019-11-12 | 南京大学 | 基于dna微阵列的蛋白质化学发光成像分析方法 |
| CN112770776B (zh) | 2018-07-30 | 2025-08-19 | 瑞德库尔有限责任公司 | 用于样品处理或分析的方法和系统 |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| EP3894590B1 (en) | 2018-12-10 | 2025-10-22 | 10X Genomics, Inc. | Methods of using master / copy arrays for spatial detection |
| CA3122569A1 (en) | 2018-12-13 | 2020-06-18 | President And Fellows Of Harvard College | Amplification methods and systems for merfish and other applications |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| SG11202112059TA (en) * | 2019-05-24 | 2021-12-30 | Nat Univ Singapore | Probe assay for the detection of biomolecules |
| KR102221191B1 (ko) * | 2019-05-30 | 2021-03-03 | 한국과학기술연구원 | 표적 핵산 검출 구조체 및 신호 증폭 제제를 포함하는 표적 핵산 검출 키트 |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| WO2020240025A1 (en) | 2019-05-31 | 2020-12-03 | Cartana Ab | Method of detecting target nucleic acid molecules |
| EP3754028A1 (en) * | 2019-06-18 | 2020-12-23 | Apollo Life Sciences GmbH | Method of signal encoding of analytes in a sample |
| CN110484440A (zh) * | 2019-08-30 | 2019-11-22 | 希格诺(南京)生物科技有限公司 | 一种核酸荧光原位检测装置及方法 |
| CN111593095B (zh) * | 2019-09-30 | 2023-04-18 | 天津大学 | 基于SiO2核酸探针和杂交链信号放大的Ag+检测方法 |
| US20210095333A1 (en) * | 2019-09-30 | 2021-04-01 | X Development Llc | Quantification of molecules using nucleic acid strand displacement detection |
| CA3162326A1 (en) * | 2019-10-16 | 2021-04-22 | Sarah SHULTZABERGER | Systems and methods for detecting multiple analytes |
| KR102452343B1 (ko) * | 2019-10-18 | 2022-10-07 | 고려대학교 산학협력단 | 하이드로젤 입자 기반 표적물질 비색 검출법 |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| CN112877402A (zh) * | 2019-11-30 | 2021-06-01 | 闽江学院 | 一种目标物催化诱导自组装dna网状纳米材料的制备与应用 |
| GB201919032D0 (en) * | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| WO2021133849A1 (en) | 2019-12-23 | 2021-07-01 | 10X Genomics, Inc. | Methods for spatial analysis using rna-templated ligation |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| WO2021155063A1 (en) | 2020-01-29 | 2021-08-05 | Readcoor, Llc | Compositions and methods for analyte detection |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| CN111172243B (zh) * | 2020-02-05 | 2022-09-09 | 山西大同大学 | 基于杂交链式反应的荧光dna传感器及e542k基因检测方法 |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| DE102020103966B4 (de) * | 2020-02-14 | 2022-03-03 | Testo bioAnalytics GmbH | Sondenkomplex zum spezifischen Nachweis von wenigstens einer Substanz, korrespondierendes Verfahren und Verwendung |
| EP4107284A1 (en) | 2020-02-17 | 2022-12-28 | 10X Genomics, Inc. | In situ analysis of chromatin interaction |
| EP4484571A3 (en) | 2020-02-21 | 2025-03-19 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| AU2021265719A1 (en) | 2020-03-06 | 2022-11-03 | California Institute Of Technology | Analysis of target molecules within a sample via hybridization chain reaction |
| GB202004469D0 (en) | 2020-03-27 | 2020-05-13 | Olink Proteomics Ab | Controls for proximity detection assays |
| CN111411146B (zh) * | 2020-03-31 | 2024-01-16 | 宜昌美光硅谷生命科技股份有限公司 | 一种检测和定量单拷贝染色体外dna或rna的方法 |
| US20230151405A1 (en) * | 2020-04-15 | 2023-05-18 | The Penn State Research Foundation | Biodegradable dna-alginate conjugate for reversible protein and cell labeling and imaging |
| ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
| US20210340621A1 (en) * | 2020-04-22 | 2021-11-04 | Readcoor, Llc | Methods and systems for sequencing |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| EP4553168A3 (en) | 2020-05-22 | 2025-08-27 | 10x Genomics, Inc. | Spatial analysis to detect sequence variants |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| JP7482506B2 (ja) * | 2020-06-12 | 2024-05-14 | 学校法人東邦大学 | 短鎖ヘアピンDNAを用いた改良型in situ ハイブリダイゼーション反応 |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| CA3181725A1 (en) * | 2020-06-18 | 2021-12-23 | Andreas Geipel | Multiplex method for detecting different analytes in a sample |
| EP4172362B1 (en) | 2020-06-25 | 2024-09-18 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| CN114480592A (zh) * | 2020-10-27 | 2022-05-13 | 重庆医科大学 | 基于UDG介导PCR、磁性纳米富集和链置换反应的SNPs检测技术 |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4012046A1 (en) | 2020-12-11 | 2022-06-15 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| GB202100760D0 (en) * | 2021-01-20 | 2021-03-03 | Qbiotix Ltd | Target-dependent polymerisation of oligonucleotides |
| US11873485B2 (en) | 2021-01-26 | 2024-01-16 | California Institute Of Technology | Allosteric conditional guide RNAs for cell-selective regulation of CRISPR/Cas |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| EP4428246B1 (en) | 2021-04-14 | 2025-12-24 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| EP4582555A3 (en) | 2021-06-03 | 2025-10-22 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| US20250084476A1 (en) * | 2021-07-28 | 2025-03-13 | University Of Washington | Multiplexed in situ signal amplification with splint ligation extension (slx) probes |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| CA3224093A1 (en) | 2021-07-30 | 2023-02-02 | Jorge Ivan Hernandez Neuta | Methods and compositions for synchronizing reactions in situ |
| US12460251B2 (en) | 2021-08-03 | 2025-11-04 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid molecules |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| US12529096B2 (en) | 2021-08-03 | 2026-01-20 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid structures |
| EP4381095A1 (en) | 2021-08-03 | 2024-06-12 | 10X Genomics, Inc. | Nucleic acid concatemers and methods for stabilizing and/or compacting the same |
| US12435364B2 (en) | 2021-08-16 | 2025-10-07 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| WO2023047340A1 (en) * | 2021-09-24 | 2023-03-30 | Crispr Therapeutics Ag | HIGH PURITY gRNA SYNTHESIS PROCESS |
| WO2023059549A1 (en) * | 2021-10-04 | 2023-04-13 | The Regents Of The University Of California | Nucleic acid implementation of multilayer perceptrons |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| CN114252602B (zh) * | 2021-12-22 | 2023-09-12 | 清华大学深圳国际研究生院 | 微流控芯片、基于微流控芯片的检测系统及细菌的检测方法 |
| US20250101511A1 (en) * | 2022-01-17 | 2025-03-27 | University Of Washington | Oligo-mediated deposition of functional molecules for spatially resolved genomics, transcriptomics, and proteomics |
| CA3252139A1 (en) * | 2022-02-21 | 2023-08-24 | Kanvas Biosciences, Inc. | HIGHLY MULTIPLEXED DETECTION OF GENE EXPRESSION BY CHAIN HYBRIDIZATION REACTION |
| EP4490737A1 (en) | 2022-03-08 | 2025-01-15 | 10X Genomics, Inc. | In situ code design methods for minimizing optical crowding |
| AU2023243205A1 (en) | 2022-04-01 | 2024-09-19 | 10X Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| EP4519453A1 (en) | 2022-05-06 | 2025-03-12 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| CN119213143A (zh) | 2022-05-11 | 2024-12-27 | 10X基因组学有限公司 | 用于原位测序的组合物和方法 |
| WO2023245190A1 (en) | 2022-06-17 | 2023-12-21 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| WO2024040060A1 (en) | 2022-08-16 | 2024-02-22 | 10X Genomics, Inc. | Ap50 polymerases and uses thereof |
| WO2024102809A1 (en) | 2022-11-09 | 2024-05-16 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
| WO2025029831A1 (en) | 2023-07-31 | 2025-02-06 | 10X Genomics, Inc. | Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification |
| JP2025058469A (ja) * | 2023-09-28 | 2025-04-09 | 国立大学法人九州大学 | プローブの設計方法、プローブの設計システム、及びプローブの設計プログラム |
| US20260015659A1 (en) * | 2024-07-11 | 2026-01-15 | SequLITE Genomics US, Inc. | Method for Nucleic Acid Sequence Detection and Sequencing Method |
| CN119685325B (zh) * | 2025-02-26 | 2025-05-23 | 绿城农科检测技术有限公司 | 一种具有磁性纳米粒子的三角多价适配体引发链的制备方法 |
| CN120361183A (zh) * | 2025-04-28 | 2025-07-25 | 山西锦波生物医药股份有限公司 | Grft及其融合蛋白在副粘病毒或新发冠状病毒感染中的应用 |
| CN121160891A (zh) * | 2025-09-17 | 2025-12-19 | 上海市第十人民医院 | 一种基于荧光指纹特征的多重病原菌联合鉴别方法 |
| CN121653230A (zh) * | 2026-02-06 | 2026-03-13 | 中国科学技术大学 | 高通量原位检测生物样品内核酸分子的探针组、试剂盒和方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014182528A2 (en) | 2013-04-30 | 2014-11-13 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding |
| WO2015118029A1 (en) | 2014-02-04 | 2015-08-13 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (hcr) |
Family Cites Families (263)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4123610A (en) | 1977-03-09 | 1978-10-31 | The United States Government | Nucleic acid crosslinking agent and affinity inactivation of nucleic acids therewith |
| US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
| US4981985A (en) | 1987-10-20 | 1991-01-01 | Trustees Of The University Of Pennsylvania | Synthesis of photolabile chelators for multivalent cations |
| US4886741A (en) | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
| US4844617A (en) | 1988-01-20 | 1989-07-04 | Tencor Instruments | Confocal measuring microscope with automatic focusing |
| US5151189A (en) * | 1990-09-17 | 1992-09-29 | Gelman Sciences, Inc. | Cationic charge modified microporous membrane |
| JPH04268359A (ja) | 1991-02-22 | 1992-09-24 | Taiho Yakuhin Kogyo Kk | 刺激応答性ヒドロゲル及びその製造方法 |
| US5424413A (en) | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| AU3664693A (en) | 1992-02-13 | 1993-09-03 | Bio-Metric Systems, Inc. | Immobilization of chemical species in crosslinked matrices |
| US5594235A (en) | 1993-06-17 | 1997-01-14 | Ultrapointe Corporation | Automated surface acquisition for a confocal microscope |
| US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| SE9400522D0 (sv) * | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5830708A (en) | 1995-06-06 | 1998-11-03 | Advanced Tissue Sciences, Inc. | Methods for production of a naturally secreted extracellular matrix |
| US6284284B1 (en) | 1995-06-06 | 2001-09-04 | Advanced Tissue Sciences, Inc. | Compositions and methods for production and use of an injectable naturally secreted extracellular matrix |
| CA2256700A1 (en) | 1996-06-06 | 1997-12-11 | Lynx Therapeutics, Inc. | Sequencing by ligation of encoded adaptors |
| JP4532608B2 (ja) | 1997-02-07 | 2010-08-25 | オリンパス株式会社 | 走査型プローブ顕微鏡を用いた核酸検出方法 |
| US5948617A (en) | 1997-06-13 | 1999-09-07 | Biospeparations, Inc. | Methods of in situ hybridization |
| US6083726A (en) * | 1998-02-03 | 2000-07-04 | Lucent Technologies, Inc. | Methods for polynucleotide synthesis and articles for polynucleotide hybridization |
| US6068979A (en) | 1998-04-22 | 2000-05-30 | Lumigen, Inc. | Simplified sequential chemiluminescent detection |
| JP3662850B2 (ja) | 1998-06-24 | 2005-06-22 | イルミナ インコーポレイテッド | 微小球を有するアレイセンサーのデコード |
| JP2002531056A (ja) | 1998-08-07 | 2002-09-24 | セレイ, エルエルシー | 遺伝子分析におけるゲルマイクロドロップ |
| US6232067B1 (en) | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
| DE60045917D1 (de) * | 1999-02-23 | 2011-06-16 | Caliper Life Sciences Inc | Sequenzierung durch inkorporation |
| EP1171636A2 (en) | 1999-04-22 | 2002-01-16 | Albert Einstein College Of Medicine Of Yeshiva University | Assay of gene expression patterns by multi-fluor fish |
| US20020015952A1 (en) | 1999-07-30 | 2002-02-07 | Anderson Norman G. | Microarrays and their manufacture by slicing |
| CA2386151A1 (en) | 1999-10-12 | 2001-04-19 | Marc Madou | Reactive polymeric valve, dispensing devices and methods using same |
| AU1774501A (en) | 1999-11-17 | 2001-05-30 | Research Foundation Of State University Of New York, The | Three dimensional data storage device and method for reading |
| US7115738B2 (en) | 2000-03-14 | 2006-10-03 | Active Motif | Hydroxyproline/phosphono oligonucleotide analogues, methods of synthesis and methods of use |
| US7033758B2 (en) * | 2000-06-02 | 2006-04-25 | Bayer Corporation | Highly sensitive gene detection and localization using in situ branched-DNA hybridization |
| US8548745B2 (en) | 2000-06-08 | 2013-10-01 | The Regents Of The University Of California | Visual-servoing optical microscopy |
| US7613571B2 (en) | 2000-07-28 | 2009-11-03 | Doyle Michael D | Method and system for the multidimensional morphological reconstruction of genome expression activity |
| US6797152B2 (en) | 2000-07-31 | 2004-09-28 | California Institute Of Technology | Sensors and sensing methods for detecting analytes based on changes in pKa of a sensing polymer |
| EP1368369A4 (en) | 2000-11-15 | 2006-02-22 | Hoffmann La Roche | METHOD AND REAGENTS FOR IDENTIFYING RARE FOETAL CELLS IN THE MATERIAL CIRCUIT |
| AU3090102A (en) | 2000-12-14 | 2002-06-24 | Gen Probe Inc | Method and kit for enhancing the association rates of polynucleotides |
| EP2801624B1 (en) | 2001-03-16 | 2019-03-06 | Singular Bio, Inc | Arrays and methods of use |
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| US20060248349A1 (en) | 2001-09-24 | 2006-11-02 | Rathjen Alicemarie G | Method for preparing and using personal and genetic profiles |
| US20030148335A1 (en) | 2001-10-10 | 2003-08-07 | Li Shen | Detecting targets by unique identifier nucleotide tags |
| GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
| US8013134B2 (en) | 2001-11-23 | 2011-09-06 | Olink Ab | Kit for proximity probing with multivalent proximity probes |
| US20090246879A1 (en) | 2002-12-20 | 2009-10-01 | Callida Genomics | Materials and Methods Relating to Nano-Tags and Nano-Brands |
| WO2005003375A2 (en) | 2003-01-29 | 2005-01-13 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| EP2365095A1 (en) | 2003-02-26 | 2011-09-14 | Callida Genomics, Inc. | Random array DNA analysis by hybridization |
| US7385043B1 (en) | 2003-04-30 | 2008-06-10 | The Public Health Research Institute Of The City Of New York, Inc. | Homogeneous multiplex screening assays and kits |
| CN102135661B (zh) | 2003-05-20 | 2014-06-04 | 卢西德有限公司 | 用于对患者身体所选位置进行成像的共焦显微镜 |
| US7255994B2 (en) | 2003-06-10 | 2007-08-14 | Applera Corporation | Ligation assay |
| CN1580283A (zh) | 2003-08-13 | 2005-02-16 | 清华大学 | 一种检测核酸分子的方法 |
| US7193054B2 (en) | 2003-08-26 | 2007-03-20 | University Of Rochester | Nanofabrication using actin filaments |
| US20050064435A1 (en) | 2003-09-24 | 2005-03-24 | Xing Su | Programmable molecular barcodes |
| WO2005049851A2 (en) | 2003-11-14 | 2005-06-02 | University Of Utah Research Foundation | Methods, articles, and compositions for identifying oligonucleotides |
| US7381529B2 (en) | 2003-12-31 | 2008-06-03 | Intel Corporation | Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes |
| JP2007526772A (ja) | 2004-02-27 | 2007-09-20 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | インサイチュー配列決定用ポロニー蛍光ビーズ |
| US20050191687A1 (en) * | 2004-02-27 | 2005-09-01 | Tianxin Wang | Method for multiplexed analyte detection |
| WO2005098049A2 (en) | 2004-03-25 | 2005-10-20 | California Institute Of Technology | Hybridization chain reaction |
| WO2006076017A2 (en) | 2004-04-30 | 2006-07-20 | Applera Corporation | Methods and kits for identifying target nucleotides in mixed populations |
| US20060024711A1 (en) | 2004-07-02 | 2006-02-02 | Helicos Biosciences Corporation | Methods for nucleic acid amplification and sequence determination |
| US7253947B2 (en) | 2004-10-07 | 2007-08-07 | New York University | Portable automated confocal microscope |
| CN101916359B (zh) | 2005-01-27 | 2012-06-20 | 剑桥研究和仪器设备股份有限公司 | 把样本的不同区域分类到相应类别的方法和设备 |
| WO2006084132A2 (en) | 2005-02-01 | 2006-08-10 | Agencourt Bioscience Corp. | Reagents, methods, and libraries for bead-based squencing |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| ITRM20050068A1 (it) * | 2005-02-17 | 2006-08-18 | Istituto Naz Per Le Malattie I | Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine. |
| US20060234261A1 (en) * | 2005-03-08 | 2006-10-19 | Pierce Niles A | Colorimetric readout of hybridization chain reaction |
| US7727721B2 (en) | 2005-03-08 | 2010-06-01 | California Institute Of Technology | Hybridization chain reaction amplification for in situ imaging |
| US8252754B2 (en) * | 2005-03-21 | 2012-08-28 | The Trustees Of Columbia University In The City Of New York | Neuronal pain pathway |
| US20070020650A1 (en) | 2005-04-01 | 2007-01-25 | Avak Kahvejian | Methods for detecting proteins |
| WO2007086900A2 (en) | 2005-04-08 | 2007-08-02 | Mycosol, Inc. | Stilbazium research assays |
| EP1871896B1 (en) | 2005-04-12 | 2015-06-03 | Olink AB | Circle probes and their use in the identification of biomolecules |
| US11561951B2 (en) | 2005-05-16 | 2023-01-24 | Panvia Future Technologies, Inc. | Multidimensional associative memory and data searching |
| US7601499B2 (en) | 2005-06-06 | 2009-10-13 | 454 Life Sciences Corporation | Paired end sequencing |
| US7842793B2 (en) | 2005-06-14 | 2010-11-30 | The California Institute Of Technology | Methods of making nucleic acid nanostructures |
| JP5331476B2 (ja) | 2005-06-15 | 2013-10-30 | カリダ・ジェノミックス・インコーポレイテッド | 遺伝子解析および化学解析用の単分子アレイ |
| ES2511218T5 (es) | 2005-06-20 | 2017-12-07 | Advanced Cell Diagnostics, Inc. | Kits y productos para detección de ácidos nucleicos en células individuales y para identificación de células raras en grandes poblaciones de células heterogéneas |
| EP1920072A2 (en) | 2005-07-01 | 2008-05-14 | Dako Denmark A/S | In situ hybridization detection method |
| US8486621B2 (en) | 2005-08-11 | 2013-07-16 | Cornell Research Foundation, Inc. | Nucleic acid-based matrixes |
| CN1959384A (zh) | 2005-11-01 | 2007-05-09 | 河南省生物工程技术研究中心 | 一种三维网格检测技术 |
| US20070190543A1 (en) | 2005-11-14 | 2007-08-16 | Applera Corporation | Coded Molecules for Detecting Target Analytes |
| EP1963531B1 (en) | 2005-12-23 | 2011-09-21 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| US8986926B2 (en) | 2005-12-23 | 2015-03-24 | Nanostring Technologies, Inc. | Compositions comprising oriented, immobilized macromolecules and methods for their preparation |
| US7864996B2 (en) | 2006-02-17 | 2011-01-04 | Lucid, Inc. | System for macroscopic and confocal imaging of tissue |
| WO2007106402A2 (en) * | 2006-03-10 | 2007-09-20 | President And Fellows Of Harvard College | Methods and apparatus for near field irradiation |
| GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| US20070231823A1 (en) * | 2006-03-23 | 2007-10-04 | Mckernan Kevin J | Directed enrichment of genomic DNA for high-throughput sequencing |
| RU2394915C2 (ru) | 2006-03-24 | 2010-07-20 | Александр Борисович Четверин | Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления |
| US8975216B2 (en) | 2006-03-30 | 2015-03-10 | Pacific Biosciences Of California | Articles having localized molecules disposed thereon and methods of producing same |
| EP3722409A1 (en) | 2006-03-31 | 2020-10-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| CN101495654A (zh) | 2006-04-19 | 2009-07-29 | 阿普里拉股份有限公司 | 无凝胶珠基测序的试剂、方法和文库 |
| JP5081232B2 (ja) | 2006-05-22 | 2012-11-28 | ナノストリング テクノロジーズ, インコーポレイテッド | ナノレポーターを分析するためのシステムおよび方法 |
| US7964347B2 (en) | 2006-06-15 | 2011-06-21 | Krassen Dimitrov | Labels for electronic detection of individual molecules and methods for their detection |
| US20080038163A1 (en) | 2006-06-23 | 2008-02-14 | Applera Corporation | Systems and Methods for Cooling in Biological Analysis Instruments |
| US7745129B1 (en) | 2006-07-12 | 2010-06-29 | Kenneth David Schatz | Methods for sequencing of a necleic acid |
| WO2008033848A2 (en) | 2006-09-11 | 2008-03-20 | The Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University | Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing |
| US20090208965A1 (en) | 2006-10-25 | 2009-08-20 | Ikonisys, Inc. | Automated method for detecting cancers and high grade hyperplasias |
| EP2061588B1 (en) | 2006-11-06 | 2016-07-13 | Clondiag GmbH | Device and process for binding assays |
| US9201063B2 (en) | 2006-11-16 | 2015-12-01 | General Electric Company | Sequential analysis of biological samples |
| US7655898B2 (en) | 2006-11-30 | 2010-02-02 | Cambridge Research & Instrumentation, Inc. | Optical filter assembly with selectable bandwidth and rejection |
| WO2008069973A2 (en) | 2006-12-01 | 2008-06-12 | The Trustees Of Columbia University In The City Of New York | Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| US20080269068A1 (en) * | 2007-02-06 | 2008-10-30 | President And Fellows Of Harvard College | Multiplex decoding of sequence tags in barcodes |
| AU2008223544B2 (en) | 2007-03-02 | 2014-06-05 | Dupont Nutrition Biosciences Aps | Cultures with improved phage resistance |
| US8145677B2 (en) | 2007-03-27 | 2012-03-27 | Faleh Jassem Al-Shameri | Automated generation of metadata for mining image and text data |
| US8415102B2 (en) | 2007-04-10 | 2013-04-09 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
| WO2008144562A1 (en) * | 2007-05-16 | 2008-11-27 | California Institute Of Technology | A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways |
| ES2959127T3 (es) | 2007-06-19 | 2024-02-20 | Hoffmann La Roche | Secuenciación de ácidos nucleicos de alto rendimiento por expansión |
| US7534991B2 (en) | 2007-07-10 | 2009-05-19 | Cambridge Research & Instrumentation, Inc. | Athermalized birefringent filter apparatus and method |
| US20110294135A1 (en) | 2007-08-02 | 2011-12-01 | BioDesic, LLC | Compositions and methods for analyte detection and quantitation |
| JP2010539982A (ja) | 2007-10-01 | 2010-12-24 | アプライド バイオシステムズ, エルエルシー | チェイスライゲーション配列決定法 |
| AU2008308457A1 (en) | 2007-10-04 | 2009-04-09 | Halcyon Molecular | Sequencing nucleic acid polymers with electron microscopy |
| US20090139311A1 (en) | 2007-10-05 | 2009-06-04 | Applied Biosystems Inc. | Biological Analysis Systems, Devices, and Methods |
| EP2051051B1 (en) | 2007-10-16 | 2020-06-03 | Cambridge Research & Instrumentation, Inc. | Spectral imaging system with dynamic optical correction |
| US20110020291A1 (en) | 2007-11-17 | 2011-01-27 | Debrabrata Banerjee | Use of stem cells for wound healing |
| CN101586150B (zh) | 2008-05-23 | 2016-09-28 | 陕西佰美基因股份有限公司 | 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途 |
| US8199999B2 (en) | 2008-06-17 | 2012-06-12 | Cambridge Research & Instrumentation, Inc. | Image classifier training |
| WO2010011961A2 (en) | 2008-07-25 | 2010-01-28 | University Of Georgia Research Foundation, Inc. | Prokaryotic rnai-like system and methods of use |
| US20120040397A1 (en) | 2008-08-05 | 2012-02-16 | Dan Luo | Photo-Crosslinked Nucleic Acid Hydrogels |
| US8519115B2 (en) | 2008-08-14 | 2013-08-27 | Nanostring Technologies, Inc. | Stable nanoreporters |
| WO2010030818A2 (en) | 2008-09-10 | 2010-03-18 | University Of Medicine And Dentistry Of New Jersey | IMAGING INDIVIDUAL mRNA MOLECULES USING MULTIPLE SINGLY LABELED PROBES |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US20100076057A1 (en) | 2008-09-23 | 2010-03-25 | Northwestern University | TARGET DNA INTERFERENCE WITH crRNA |
| US20100087325A1 (en) | 2008-10-07 | 2010-04-08 | Illumina, Inc. | Biological sample temperature control system and method |
| RU2529804C2 (ru) * | 2008-10-23 | 2014-09-27 | Конинклейке Филипс Электроникс, Н.В. | Молекулярная визуализация |
| WO2010054108A2 (en) | 2008-11-06 | 2010-05-14 | University Of Georgia Research Foundation, Inc. | Cas6 polypeptides and methods of use |
| US8309306B2 (en) * | 2008-11-12 | 2012-11-13 | Nodality, Inc. | Detection composition |
| WO2010080134A1 (en) | 2008-12-19 | 2010-07-15 | President And Fellows Of Harvard College | Particle-assisted nucleic acid sequencing |
| JP4528345B2 (ja) | 2009-01-28 | 2010-08-18 | シャープ株式会社 | 動画再生装置、動画再生方法、動画再生方法をコンピュータで実現するためのプログラム及びそのプログラムを記録した記録媒体 |
| JP5277082B2 (ja) | 2009-06-15 | 2013-08-28 | 株式会社日立ハイテクノロジーズ | 蛍光分析方法 |
| FR2948475A1 (fr) | 2009-07-24 | 2011-01-28 | Bionext | Procede de caracterisation d'objets tridimensionnels |
| US8431151B2 (en) * | 2009-08-06 | 2013-04-30 | Syracuse University | Antimicrobial nanostructured hydrogel web containing silver |
| WO2011047087A2 (en) | 2009-10-13 | 2011-04-21 | Nanostring Technologies, Inc. | Protein detection via nanoreporters |
| US20110104693A1 (en) | 2009-11-03 | 2011-05-05 | High Throughput Genomics, Inc. | QUANTITATIVE NUCLEASE PROTECTION SEQUENCING (qNPS) |
| EP2531220B1 (en) | 2009-12-18 | 2018-05-02 | President and Fellows of Harvard College | Active scaffolds for on-demand drug and cell delivery |
| US8632976B2 (en) | 2010-01-19 | 2014-01-21 | Agdia | Amplification of TRP1 for specific detection of Phytophthora ramorum |
| EP2516681B1 (en) | 2010-02-11 | 2017-10-18 | Nanostring Technologies, Inc | Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation |
| WO2011100617A2 (en) | 2010-02-12 | 2011-08-18 | Life Technologies Corporation | Nucleic acid, biomolecule and polymer identifier codes |
| US10267808B2 (en) | 2010-03-08 | 2019-04-23 | California Institute Of Technology | Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells |
| US10087431B2 (en) | 2010-03-10 | 2018-10-02 | The Regents Of The University Of California | Methods of generating nucleic acid fragments |
| GB201004292D0 (en) | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
| HRP20151294T1 (hr) | 2010-04-05 | 2016-03-11 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| US8462981B2 (en) | 2010-04-07 | 2013-06-11 | Cambridge Research & Instrumentation, Inc. | Spectral unmixing for visualization of samples |
| SG185481A1 (en) | 2010-05-10 | 2012-12-28 | Univ California | Endoribonuclease compositions and methods of use thereof |
| WO2011143583A1 (en) | 2010-05-13 | 2011-11-17 | Illumina, Inc. | Binding assays for markers |
| WO2012005595A2 (en) | 2010-07-09 | 2012-01-12 | Wouter Leonard De Laat | V3-d genomic region of interest sequencing strategies |
| US9541504B2 (en) | 2010-08-05 | 2017-01-10 | Cambridge Research & Instrumentation, Inc. | Enhancing visual assessment of samples |
| EP2627781B1 (en) | 2010-10-15 | 2017-02-22 | Olink Bioscience AB | Dynamic range methods |
| CN104849472A (zh) | 2010-10-21 | 2015-08-19 | 领先细胞医疗诊断有限公司 | 用于原位检测核酸的超灵敏方法 |
| WO2012056440A1 (en) | 2010-10-28 | 2012-05-03 | Nanodoc Ltd. | COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA |
| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| EP2633081B1 (en) | 2010-10-29 | 2017-01-11 | Olink Bioscience AB | Proximity ligation technology for western blot applications |
| WO2012067911A1 (en) | 2010-11-16 | 2012-05-24 | Nabsys, Inc. | Methods for sequencing a biomolecule by detecting relative positions of hybridized probes |
| EP2675916B1 (en) | 2011-02-15 | 2016-08-24 | Leica Biosystems Newcastle Limited | METHOD FOR LOCALIZED IN SITU DETECTION OF mRNA |
| JP5687514B2 (ja) | 2011-02-17 | 2015-03-18 | 株式会社日立ハイテクノロジーズ | 核酸配列解析装置 |
| EP2500436B1 (en) | 2011-03-17 | 2016-05-25 | Institut Pasteur | Method, probe and kit for DNA in situ hybridation and use thereof |
| US20120252686A1 (en) | 2011-03-31 | 2012-10-04 | Good Start Genetics | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US8946389B2 (en) * | 2011-04-25 | 2015-02-03 | University Of Washington | Compositions and methods for multiplex biomarker profiling |
| EP2705161A1 (de) | 2011-05-04 | 2014-03-12 | Genovoxx GmbH | Nukleosid-triphosphat-konjugate und methoden zu deren anwendung |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| GB201108678D0 (en) | 2011-05-24 | 2011-07-06 | Olink Ab | Multiplexed proximity ligation assay |
| WO2012166425A2 (en) | 2011-05-27 | 2012-12-06 | President And Fellows Of Harvard College | Methods of amplifying whole genome of a single cell |
| WO2012164565A1 (en) | 2011-06-01 | 2012-12-06 | Yeda Research And Development Co. Ltd. | Compositions and methods for downregulating prokaryotic genes |
| PL222511B1 (pl) | 2011-06-08 | 2016-08-31 | Międzynarodowy Inst Biologii Molekularnej I Komórkowej W Warszawie | Endorybonukleazy dsRNA |
| WO2013055995A2 (en) | 2011-10-14 | 2013-04-18 | President And Fellows Of Harvard College | Sequencing by structure assembly |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP4697020A2 (en) | 2011-12-22 | 2026-02-18 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| RU2650811C2 (ru) | 2012-02-24 | 2018-04-17 | Фред Хатчинсон Кэнсер Рисерч Сентер | Композиции и способы лечения гемоглобинопатии |
| US10941396B2 (en) * | 2012-02-27 | 2021-03-09 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
| CN108285491B (zh) | 2012-02-29 | 2021-08-10 | 桑格摩生物科学股份有限公司 | 治疗亨廷顿氏病的方法和组合物 |
| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| US9082418B2 (en) | 2012-07-16 | 2015-07-14 | Marvell International Ltd. | Methods for reading data from a storage medium using a reader and storage devices |
| JP6019120B2 (ja) | 2012-07-20 | 2016-11-02 | 株式会社Lsiメディエンス | 光応答性核酸類を含むプローブを用いた光連結方法 |
| HK1207111A1 (en) | 2012-08-03 | 2016-01-22 | 加利福尼亚大学董事会 | Methods and compositions for controlling gene expression by rna processing |
| WO2014025392A1 (en) | 2012-08-09 | 2014-02-13 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for preparing biological specimens for microscopic analysis |
| WO2014028538A2 (en) | 2012-08-13 | 2014-02-20 | William Marsh Rice University | Multiplexed in situ molecular analyses and programmable molecular probes for regulated signal amplification |
| EP2885670B1 (en) | 2012-08-15 | 2021-01-27 | Lucid, Inc. | Systems and methods for imaging tissue |
| EP2888576B1 (en) | 2012-08-21 | 2023-10-04 | Akoya Biosciences, Inc. | Visualization and measurement of cell compartments |
| CN102908119A (zh) | 2012-09-26 | 2013-02-06 | 温州医学院眼视光研究院 | 一种共焦扫描成像系统及其像差控制方法 |
| US9783841B2 (en) | 2012-10-04 | 2017-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of target nucleic acids in a cellular sample |
| EP4357457B1 (en) | 2012-10-23 | 2024-10-16 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| WO2014076209A1 (en) | 2012-11-14 | 2014-05-22 | Olink Ab | Localised rca-based amplification method |
| EP3363902B1 (en) | 2012-12-06 | 2019-11-27 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
| AU2013359262C1 (en) | 2012-12-12 | 2021-05-13 | Massachusetts Institute Of Technology | CRISPR-Cas component systems, methods and compositions for sequence manipulation |
| DK2931897T3 (en) | 2012-12-12 | 2018-02-05 | Broad Inst Inc | CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| DK2931898T3 (en) | 2012-12-12 | 2016-06-20 | Massachusetts Inst Technology | CONSTRUCTION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION WITH FUNCTIONAL DOMAINS |
| WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| CA2895155C (en) | 2012-12-17 | 2021-07-06 | President And Fellows Of Harvard College | Rna-guided human genome engineering |
| CN105103190B (zh) | 2013-01-10 | 2018-07-17 | 加利珀生命科学股份有限公司 | 全视野载玻片多光谱成像系统和方法 |
| WO2014110027A1 (en) | 2013-01-10 | 2014-07-17 | Caliper Life Sciences, Inc. | Multispectral imaging system and methods |
| EP3919505B1 (en) | 2013-01-16 | 2023-08-30 | Emory University | Uses of cas9-nucleic acid complexes |
| US9411930B2 (en) | 2013-02-01 | 2016-08-09 | The Regents Of The University Of California | Methods for genome assembly and haplotype phasing |
| EP3578666A1 (en) | 2013-03-12 | 2019-12-11 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| US9273349B2 (en) | 2013-03-14 | 2016-03-01 | Affymetrix, Inc. | Detection of nucleic acids |
| MX374090B (es) | 2013-03-14 | 2025-03-05 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico. |
| AU2014227653B2 (en) | 2013-03-15 | 2017-04-20 | The General Hospital Corporation | Using RNA-guided foki nucleases (RFNs) to increase specificity for RNA-guided genome editing |
| US20140273230A1 (en) | 2013-03-15 | 2014-09-18 | Sigma-Aldrich Co., Llc | Crispr-based genome modification and regulation |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| US9330295B2 (en) | 2013-03-15 | 2016-05-03 | Brown University | Spatial sequencing/gene expression camera |
| US10510435B2 (en) * | 2013-04-30 | 2019-12-17 | California Institute Of Technology | Error correction of multiplex imaging analysis by sequential hybridization |
| US20160122774A1 (en) | 2013-05-29 | 2016-05-05 | Cellectis | A method for producing precise dna cleavage using cas9 nickase activity |
| US20140356956A1 (en) | 2013-06-04 | 2014-12-04 | President And Fellows Of Harvard College | RNA-Guided Transcriptional Regulation |
| EP4596565A3 (en) | 2013-06-04 | 2025-11-05 | President And Fellows Of Harvard College | Rna-guided transcriptional regulation |
| EP3011030B1 (en) | 2013-06-17 | 2023-11-08 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| EP3013983B1 (en) | 2013-06-25 | 2023-02-15 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| JP2016538569A (ja) | 2013-09-20 | 2016-12-08 | カリフォルニア インスティチュート オブ テクノロジー | 無傷全組織の表現型分類のための方法 |
| US10794802B2 (en) | 2013-09-20 | 2020-10-06 | California Institute Of Technology | Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping |
| US10539772B2 (en) | 2013-10-09 | 2020-01-21 | Howard Hughes Medical Institute | Multiview light-sheet microscopy |
| EP3058091B1 (en) | 2013-10-18 | 2020-03-25 | The Broad Institute, Inc. | Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing |
| EP3080259B1 (en) | 2013-12-12 | 2023-02-01 | The Broad Institute, Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
| US10309879B2 (en) | 2014-02-21 | 2019-06-04 | Massachusetts Institute Of Technology | Expansion microscopy |
| CN105960572B (zh) | 2014-02-24 | 2019-01-22 | 索尼公司 | 用于定制的触觉反馈的智能可穿戴设备和方法 |
| JP2017517761A (ja) | 2014-05-30 | 2017-06-29 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | 大きなインタクトな組織試料を撮像するための方法及び装置 |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| WO2016007739A1 (en) | 2014-07-09 | 2016-01-14 | Caliper Life Sciences, Inc. | Pure spectrum extraction from biological samples |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| US10872679B2 (en) | 2014-07-17 | 2020-12-22 | California Institute Of Technology | Multiplex analysis of molecules in single cells by image correlation |
| CN112029826B (zh) | 2014-07-30 | 2025-03-14 | 哈佛学院院长及董事 | 用于测定核酸的系统和方法 |
| US11091810B2 (en) | 2015-01-27 | 2021-08-17 | BioSpyder Technologies, Inc. | Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology |
| US20160108458A1 (en) | 2014-10-06 | 2016-04-21 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplexed detection and quantification of nucleic acids in single-cells |
| CA2968376C (en) | 2014-11-21 | 2020-06-23 | Nanostring Technologies, Inc. | Enzyme- and amplification-free sequencing |
| JP7144142B2 (ja) | 2014-11-24 | 2022-09-29 | ナノストリング テクノロジーズ,インコーポレイティド | 遺伝子精製および画像化のための方法および装置 |
| CN107209934B (zh) | 2014-12-03 | 2021-03-30 | 文塔纳医疗系统公司 | 用于定量分析异质生物标志物分布的方法、系统和装置 |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US10526649B2 (en) | 2015-04-14 | 2020-01-07 | Massachusetts Institute Of Technology | Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information |
| US11408890B2 (en) | 2015-04-14 | 2022-08-09 | Massachusetts Institute Of Technology | Iterative expansion microscopy |
| US9607375B2 (en) | 2015-06-03 | 2017-03-28 | Eileen B. Gallagher | Biological data annotation and visualization |
| WO2017015099A1 (en) | 2015-07-17 | 2017-01-26 | Nanostring Technologies, Inc. | Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue |
| EP3325649B1 (en) | 2015-07-17 | 2024-01-03 | Nanostring Technologies, Inc. | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
| WO2017019481A1 (en) | 2015-07-24 | 2017-02-02 | The Johns Hopkins University | Compositions and methods of rna analysis |
| US11929149B2 (en) | 2015-08-06 | 2024-03-12 | Arc Bio, Llc | Systems and methods for genomic analysis |
| CA2994958C (en) | 2015-08-07 | 2024-02-13 | Massachusetts Institute Of Technology | Nanoscale imaging of proteins and nucleic acids via expansion microscopy |
| EP3332258B1 (en) | 2015-08-07 | 2020-01-01 | Massachusetts Institute of Technology | Protein retention expansion microscopy |
| US11214661B2 (en) | 2015-09-17 | 2022-01-04 | Massachusetts Institute Of Technology | Three-dimensional nanofabrication by patterning of hydrogels |
| US10802262B2 (en) | 2015-10-29 | 2020-10-13 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and systems for imaging a biological sample |
| JP6882282B2 (ja) | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 三次元核酸含有マトリックスの立体撮像のための方法と装置 |
| WO2017079382A1 (en) | 2015-11-03 | 2017-05-11 | President And Fellows Of Harvard College | Systems and methods for processing spatially related sequence data received from a sequencing device |
| CN108700460B (zh) | 2015-12-21 | 2020-11-03 | 威里利生命科学有限责任公司 | 成像系统和成像方法 |
| US11254974B2 (en) | 2016-02-10 | 2022-02-22 | The Board Of Trustees Of The Leland Stanford Junior University | RNA fixation and detection in clarity-based hydrogel tissue |
| US20210207131A1 (en) | 2016-02-18 | 2021-07-08 | President And Fellows Of Harvard College | Multiplex Alteration of Cells Using a Pooled Nucleic Acid Library and Analysis Thereof |
| US11360025B2 (en) | 2016-02-22 | 2022-06-14 | Miltenyi Biotec B.V. & Co. KG | Automated analysis tool for biological specimens |
| US20170253918A1 (en) | 2016-03-01 | 2017-09-07 | Expansion Technologies | Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics |
| WO2017161251A1 (en) | 2016-03-17 | 2017-09-21 | President And Fellows Of Harvard College | Methods for detecting and identifying genomic nucleic acids |
| CN109415761B (zh) | 2016-04-25 | 2022-09-20 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
| US20180052081A1 (en) | 2016-05-11 | 2018-02-22 | Expansion Technologies | Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics |
| EP4674976A3 (en) | 2016-05-16 | 2026-03-25 | Bruker Spatial Biology, Inc. | Methods for detecting target nucleic acids in a sample |
| EP3252452A1 (en) | 2016-05-25 | 2017-12-06 | The Board of Trustees of the Leland Stanford Junior University | Method for imaging and analysis of a biological specimen |
| IL324487A (en) * | 2016-07-05 | 2026-01-01 | California Inst Of Techn | Hybridization chain reaction based on a minimal initiator |
| CN109789228B (zh) | 2016-07-27 | 2022-10-21 | 斯坦福大学托管董事会 | 高度复用荧光成像 |
| US12421540B2 (en) | 2016-08-01 | 2025-09-23 | California Institute Of Technology | Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism |
| CA3034617A1 (en) | 2016-08-30 | 2018-03-08 | California Institute Of Technology | Immunohistochemistry via hybridization chain reaction |
| US20190276881A1 (en) | 2016-11-08 | 2019-09-12 | President And Fellows Of Harvard College | Multiplexed imaging using merfish, expansion microscopy, and related technologies |
| CN116732143A (zh) | 2016-11-08 | 2023-09-12 | 哈佛学院院长及董事 | 基质印迹和清除 |
| KR102313431B1 (ko) | 2016-11-21 | 2021-10-18 | 나노스트링 테크놀로지스, 인크. | 화학적 조성물 및 이것을 사용하는 방법 |
| CN117187346A (zh) * | 2017-03-31 | 2023-12-08 | 乌尔蒂维尤股份有限公司 | Dna-抗原交换和扩增 |
| US11180804B2 (en) | 2017-07-25 | 2021-11-23 | Massachusetts Institute Of Technology | In situ ATAC sequencing |
| US11753676B2 (en) | 2017-10-11 | 2023-09-12 | Expansion Technologies | Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy |
| WO2019103996A1 (en) | 2017-11-21 | 2019-05-31 | Expansion Technologies | Expansion microscopy compatible and multiplexed in situ hybridization of formalin fixed paraffin embedded tissue sections for spatially resolved transcriptomics |
| US20210115504A1 (en) | 2017-12-08 | 2021-04-22 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridization of probes |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014182528A2 (en) | 2013-04-30 | 2014-11-13 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding |
| WO2015118029A1 (en) | 2014-02-04 | 2015-08-13 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (hcr) |
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