CN116200465A - 用于原位分子检测的杂交链反应方法 - Google Patents

用于原位分子检测的杂交链反应方法 Download PDF

Info

Publication number
CN116200465A
CN116200465A CN202211146810.9A CN202211146810A CN116200465A CN 116200465 A CN116200465 A CN 116200465A CN 202211146810 A CN202211146810 A CN 202211146810A CN 116200465 A CN116200465 A CN 116200465A
Authority
CN
China
Prior art keywords
hcr
probe
initiator
sample
fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211146810.9A
Other languages
English (en)
Chinese (zh)
Inventor
E·R·道哈瑟
G·M·丘奇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of CN116200465A publication Critical patent/CN116200465A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202211146810.9A 2016-04-25 2017-04-25 用于原位分子检测的杂交链反应方法 Pending CN116200465A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662326959P 2016-04-25 2016-04-25
US62/326,959 2016-04-25
CN201780039335.6A CN109415761B (zh) 2016-04-25 2017-04-25 用于原位分子检测的杂交链反应方法
PCT/US2017/029333 WO2017189525A1 (en) 2016-04-25 2017-04-25 Hybridization chain reaction methods for in situ molecular detection

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201780039335.6A Division CN109415761B (zh) 2016-04-25 2017-04-25 用于原位分子检测的杂交链反应方法

Publications (1)

Publication Number Publication Date
CN116200465A true CN116200465A (zh) 2023-06-02

Family

ID=60160064

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202211146810.9A Pending CN116200465A (zh) 2016-04-25 2017-04-25 用于原位分子检测的杂交链反应方法
CN201780039335.6A Active CN109415761B (zh) 2016-04-25 2017-04-25 用于原位分子检测的杂交链反应方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201780039335.6A Active CN109415761B (zh) 2016-04-25 2017-04-25 用于原位分子检测的杂交链反应方法

Country Status (7)

Country Link
US (3) US11718874B2 (https=)
EP (2) EP4613756A3 (https=)
JP (1) JP7259182B2 (https=)
CN (2) CN116200465A (https=)
AU (2) AU2017257624B2 (https=)
CA (2) CA3210120C (https=)
WO (1) WO2017189525A1 (https=)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3604555B1 (en) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP2971184B1 (en) 2013-03-12 2019-04-17 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP4328322A3 (en) 2014-07-30 2024-05-22 President and Fellows of Harvard College Probe library construction
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US11352667B2 (en) 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
IL290679B2 (en) 2016-07-05 2023-10-01 California Inst Of Techn Hybridization chain reaction based on a minimal initiator
CN118853848A (zh) 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
EP3720972A1 (en) * 2017-12-08 2020-10-14 California Institute of Technology Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridzation of probes
CN111819288B (zh) 2018-01-26 2023-07-14 北京生命科学研究所 基于杂交链式反应的免疫信号放大方法
CN110441525A (zh) * 2018-05-02 2019-11-12 南京大学 基于dna微阵列的蛋白质化学发光成像分析方法
CN112770776B (zh) 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
EP3894583A4 (en) 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
SG11202112059TA (en) * 2019-05-24 2021-12-30 Nat Univ Singapore Probe assay for the detection of biomolecules
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
KR102221191B1 (ko) * 2019-05-30 2021-03-03 한국과학기술연구원 표적 핵산 검출 구조체 및 신호 증폭 제제를 포함하는 표적 핵산 검출 키트
CN119220641A (zh) 2019-05-31 2024-12-31 10X基因组学有限公司 检测目标核酸分子的方法
EP3754028A1 (en) * 2019-06-18 2020-12-23 Apollo Life Sciences GmbH Method of signal encoding of analytes in a sample
CN110484440A (zh) * 2019-08-30 2019-11-22 希格诺(南京)生物科技有限公司 一种核酸荧光原位检测装置及方法
CN111593095B (zh) * 2019-09-30 2023-04-18 天津大学 基于SiO2核酸探针和杂交链信号放大的Ag+检测方法
US20210095333A1 (en) * 2019-09-30 2021-04-01 X Development Llc Quantification of molecules using nucleic acid strand displacement detection
US20220356514A1 (en) * 2019-10-16 2022-11-10 Illumina, Inc. Systems and methods for detecting multiple analytes
CN114761806B (zh) * 2019-10-18 2025-05-09 高丽大学校产学协力团 基于水凝胶颗粒的靶材料的比色检测
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN112877402A (zh) * 2019-11-30 2021-06-01 闽江学院 一种目标物催化诱导自组装dna网状纳米材料的制备与应用
GB201919032D0 (en) * 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
EP4097251A1 (en) 2020-01-29 2022-12-07 10X Genomics, Inc. Compositions and methods for analyte detection
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
CN111172243B (zh) * 2020-02-05 2022-09-09 山西大同大学 基于杂交链式反应的荧光dna传感器及e542k基因检测方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
DE102020103966B4 (de) * 2020-02-14 2022-03-03 Testo bioAnalytics GmbH Sondenkomplex zum spezifischen Nachweis von wenigstens einer Substanz, korrespondierendes Verfahren und Verwendung
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
EP4484571A3 (en) 2020-02-21 2025-03-19 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
IL295981A (en) 2020-03-06 2022-10-01 California Inst Of Techn Analysis of target molecules within a sample using chain reaction hybridization
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
CN111411146B (zh) * 2020-03-31 2024-01-16 宜昌美光硅谷生命科技股份有限公司 一种检测和定量单拷贝染色体外dna或rna的方法
US20230151405A1 (en) * 2020-04-15 2023-05-18 The Penn State Research Foundation Biodegradable dna-alginate conjugate for reversible protein and cell labeling and imaging
US20210340621A1 (en) * 2020-04-22 2021-11-04 Readcoor, Llc Methods and systems for sequencing
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
JP7482506B2 (ja) * 2020-06-12 2024-05-14 学校法人東邦大学 短鎖ヘアピンＤＮＡを用いた改良型ｉｎ ｓｉｔｕ ハイブリダイゼーション反応
US12492427B2 (en) * 2020-06-18 2025-12-09 Resolve Biosciences Gmbh Multiplex method for detecting different analytes in a sample
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CN114480592A (zh) * 2020-10-27 2022-05-13 重庆医科大学 基于UDG介导PCR、磁性纳米富集和链置换反应的SNPs检测技术
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
GB202100760D0 (en) * 2021-01-20 2021-03-03 Qbiotix Ltd Target-dependent polymerisation of oligonucleotides
WO2022164796A1 (en) 2021-01-26 2022-08-04 California Institute Of Technology Allosteric conditional guide rnas for cell-selective regulation of crispr/cas
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023010085A2 (en) * 2021-07-28 2023-02-02 University Of Washington Multiplexed in situ signal amplification with splint ligation extension (slx) probes
AU2022317053B2 (en) 2021-07-30 2025-01-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12553079B2 (en) 2021-08-03 2026-02-17 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
EP4446426B1 (en) 2021-08-16 2026-03-04 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US20230220379A1 (en) * 2021-09-24 2023-07-13 Crispr Therapeutics Ag HIGH PURITY gRNA SYNTHESIS PROCESS
US20240384357A1 (en) * 2021-10-04 2024-11-21 The Regents Of The University Of California Nucleic acid implementation of multilayer perceptrons
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CN114252602B (zh) * 2021-12-22 2023-09-12 清华大学深圳国际研究生院 微流控芯片、基于微流控芯片的检测系统及细菌的检测方法
US20250101511A1 (en) * 2022-01-17 2025-03-27 University Of Washington Oligo-mediated deposition of functional molecules for spatially resolved genomics, transcriptomics, and proteomics
EP4482982A1 (en) * 2022-02-21 2025-01-01 Kanvas Biosciences, Inc Highly multiplexed detection of gene expression with hybridization chain reaction
CN118974836A (zh) 2022-03-08 2024-11-15 10X基因组学有限公司 将光学拥挤最小化的原位代码设计方法
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
JP2025058469A (ja) * 2023-09-28 2025-04-09 国立大学法人九州大学 プローブの設計方法、プローブの設計システム、及びプローブの設計プログラム
US20260015659A1 (en) * 2024-07-11 2026-01-15 SequLITE Genomics US, Inc. Method for Nucleic Acid Sequence Detection and Sequencing Method
CN119685325B (zh) * 2025-02-26 2025-05-23 绿城农科检测技术有限公司 一种具有磁性纳米粒子的三角多价适配体引发链的制备方法
CN120361183A (zh) * 2025-04-28 2025-07-25 山西锦波生物医药股份有限公司 Grft及其融合蛋白在副粘病毒或新发冠状病毒感染中的应用
CN121160891A (zh) * 2025-09-17 2025-12-19 上海市第十人民医院 一种基于荧光指纹特征的多重病原菌联合鉴别方法
CN121653230A (zh) * 2026-02-06 2026-03-13 中国科学技术大学 高通量原位检测生物样品内核酸分子的探针组、试剂盒和方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060234261A1 (en) * 2005-03-08 2006-10-19 Pierce Niles A Colorimetric readout of hybridization chain reaction
CN105392898A (zh) * 2013-04-30 2016-03-09 加州理工学院 通过顺序杂交编条形码的分子多重标记

Family Cites Families (263)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4123610A (en) 1977-03-09 1978-10-31 The United States Government Nucleic acid crosslinking agent and affinity inactivation of nucleic acids therewith
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4981985A (en) 1987-10-20 1991-01-01 Trustees Of The University Of Pennsylvania Synthesis of photolabile chelators for multivalent cations
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US4844617A (en) 1988-01-20 1989-07-04 Tencor Instruments Confocal measuring microscope with automatic focusing
US5151189A (en) * 1990-09-17 1992-09-29 Gelman Sciences, Inc. Cationic charge modified microporous membrane
JPH04268359A (ja) 1991-02-22 1992-09-24 Taiho Yakuhin Kogyo Kk 刺激応答性ヒドロゲル及びその製造方法
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
EP0585436B1 (en) 1992-02-13 2000-05-03 SurModics, Inc. Immobilization of chemical species in crosslinked matrices
US5594235A (en) 1993-06-17 1997-01-14 Ultrapointe Corporation Automated surface acquisition for a confocal microscope
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
SE9400522D0 (sv) * 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US6284284B1 (en) 1995-06-06 2001-09-04 Advanced Tissue Sciences, Inc. Compositions and methods for production and use of an injectable naturally secreted extracellular matrix
US5830708A (en) 1995-06-06 1998-11-03 Advanced Tissue Sciences, Inc. Methods for production of a naturally secreted extracellular matrix
PL331513A1 (en) 1996-06-06 1999-07-19 Lynx Therapeutics Method of sequencing, by a ligand effect, specific encoded adapters and composition containing double-string oligonucleotidic adapters
JP4532608B2 (ja) 1997-02-07 2010-08-25 オリンパス株式会社 走査型プローブ顕微鏡を用いた核酸検出方法
US5948617A (en) 1997-06-13 1999-09-07 Biospeparations, Inc. Methods of in situ hybridization
US6083726A (en) * 1998-02-03 2000-07-04 Lucent Technologies, Inc. Methods for polynucleotide synthesis and articles for polynucleotide hybridization
US6068979A (en) 1998-04-22 2000-05-30 Lumigen, Inc. Simplified sequential chemiluminescent detection
EP1090293B2 (en) 1998-06-24 2019-01-23 Illumina, Inc. Decoding of array sensors with microspheres
WO2000008212A1 (en) 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
ATE556149T1 (de) * 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
JP2002542793A (ja) 1999-04-22 2002-12-17 ザ アルバート アインシュタイン カレッジ オブ メディシン オブ イエシバ ユニバーシティ 多蛍光ｆｉｓｈによる遺伝子発現パターンのアッセイ法
US20020015952A1 (en) 1999-07-30 2002-02-07 Anderson Norman G. Microarrays and their manufacture by slicing
WO2001026708A1 (en) 1999-10-12 2001-04-19 The Ohio State University Reactive polymeric valve, dispensing devices and methods using same
AU1774501A (en) 1999-11-17 2001-05-30 Research Foundation Of State University Of New York, The Three dimensional data storage device and method for reading
AU2001247414A1 (en) 2000-03-14 2001-09-24 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
ATE364722T1 (de) * 2000-06-02 2007-07-15 Bayer Corp Verfahren zum nachweis und zur lokalisierung von genen in situ durch hybridisierung einer verzweigten dna
US8548745B2 (en) 2000-06-08 2013-10-01 The Regents Of The University Of California Visual-servoing optical microscopy
US7613571B2 (en) 2000-07-28 2009-11-03 Doyle Michael D Method and system for the multidimensional morphological reconstruction of genome expression activity
US6797152B2 (en) 2000-07-31 2004-09-28 California Institute Of Technology Sensors and sensing methods for detecting analytes based on changes in pKa of a sensing polymer
CA2428757A1 (en) 2000-11-15 2002-07-18 Roche Diagnostics Corporation Methods and reagents for identifying rare fetal cells in the maternal circulation
WO2002048404A2 (en) 2000-12-14 2002-06-20 Gen-Probe Incorporated Method and kit for enhancing the association rates of polynucleotides
EP2465943A3 (en) 2001-03-16 2012-10-03 Kalim Mir Linear polymer display
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US20060248349A1 (en) 2001-09-24 2006-11-02 Rathjen Alicemarie G Method for preparing and using personal and genetic profiles
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
JP2005509444A (ja) 2001-11-23 2005-04-14 シモン・フレデリックソン 多価近接プローブにより近接プロービングするための方法およびキット
US20090246879A1 (en) 2002-12-20 2009-10-01 Callida Genomics Materials and Methods Relating to Nano-Tags and Nano-Brands
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
CN103396933B (zh) 2003-02-26 2016-04-20 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
CN1791822A (zh) 2003-05-20 2006-06-21 卢西德有限公司 用于对患者身体所选位置进行成像的共焦显微镜
WO2005047543A2 (en) 2003-06-10 2005-05-26 Applera Corporation Ligation assay
CN1580283A (zh) 2003-08-13 2005-02-16 清华大学 一种检测核酸分子的方法
US7193054B2 (en) 2003-08-26 2007-03-20 University Of Rochester Nanofabrication using actin filaments
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
WO2005049851A2 (en) 2003-11-14 2005-06-02 University Of Utah Research Foundation Methods, articles, and compositions for identifying oligonucleotides
US7381529B2 (en) 2003-12-31 2008-06-03 Intel Corporation Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes
US20050191687A1 (en) * 2004-02-27 2005-09-01 Tianxin Wang Method for multiplexed analyte detection
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
WO2005098049A2 (en) 2004-03-25 2005-10-20 California Institute Of Technology Hybridization chain reaction
WO2006076017A2 (en) 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7253947B2 (en) 2004-10-07 2007-08-07 New York University Portable automated confocal microscope
WO2006081547A1 (en) 2005-01-27 2006-08-03 Cambridge Research And Instrumentation, Inc. Classifying image features
EP2230316A1 (en) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
ITRM20050068A1 (it) * 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine.
US7727721B2 (en) * 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
EP1865978A4 (en) * 2005-03-21 2012-01-25 Univ Columbia NEW NEURONAL PAINWAY
US20070020650A1 (en) 2005-04-01 2007-01-25 Avak Kahvejian Methods for detecting proteins
MX2007012486A (es) 2005-04-08 2007-12-13 Mycosol Inc Ensayos de investigacion sobre estilbazio.
US7989166B2 (en) 2005-04-12 2011-08-02 In Situ Rcp A/S Circle probes and their use in the identification of biomolecules
US11561951B2 (en) 2005-05-16 2023-01-24 Panvia Future Technologies, Inc. Multidimensional associative memory and data searching
CA2615323A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
DK2500439T4 (da) 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
DE602006020577D1 (de) 2005-07-01 2011-04-21 Dako Denmark As Monomere und polymere linker zur konjugierung von biologischen molekülen und anderen stoffen
US8486621B2 (en) 2005-08-11 2013-07-16 Cornell Research Foundation, Inc. Nucleic acid-based matrixes
CN1959384A (zh) 2005-11-01 2007-05-09 河南省生物工程技术研究中心 一种三维网格检测技术
WO2007120265A2 (en) 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
WO2007076128A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
JP5700911B2 (ja) 2005-12-23 2015-04-15 ナノストリング テクノロジーズ，インコーポレーテッド 配向され、固定化された巨大分子を含む組成物とその製造法
US7864996B2 (en) 2006-02-17 2011-01-04 Lucid, Inc. System for macroscopic and confocal imaging of tissue
CA2647382A1 (en) * 2006-03-10 2007-09-20 President And Fellows Of Harvard College Methods and apparatus for near field irradiation
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
WO2007111937A1 (en) * 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
RU2394915C2 (ru) 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
CN101495654A (zh) 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
CA2653095C (en) 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
US7964347B2 (en) 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
US20080038163A1 (en) 2006-06-23 2008-02-14 Applera Corporation Systems and Methods for Cooling in Biological Analysis Instruments
US7745129B1 (en) 2006-07-12 2010-06-29 Kenneth David Schatz Methods for sequencing of a necleic acid
WO2008033848A2 (en) 2006-09-11 2008-03-20 The Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing
US20090208965A1 (en) 2006-10-25 2009-08-20 Ikonisys, Inc. Automated method for detecting cancers and high grade hyperplasias
WO2008055915A2 (en) 2006-11-06 2008-05-15 Clondiag Gmbh Device and process for assays using binding members
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US7655898B2 (en) 2006-11-30 2010-02-02 Cambridge Research & Instrumentation, Inc. Optical filter assembly with selectable bandwidth and rejection
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US20080269068A1 (en) * 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
CN101688241B (zh) 2007-03-02 2015-01-21 杜邦营养生物科学有限公司 具有改善的噬菌体抗性的培养物
US8145677B2 (en) 2007-03-27 2012-03-27 Faleh Jassem Al-Shameri Automated generation of metadata for mining image and text data
CA2687292C (en) 2007-04-10 2017-07-04 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
WO2008144562A1 (en) * 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
DK2171088T3 (en) 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
US7534991B2 (en) 2007-07-10 2009-05-19 Cambridge Research & Instrumentation, Inc. Athermalized birefringent filter apparatus and method
US20110294135A1 (en) 2007-08-02 2011-12-01 BioDesic, LLC Compositions and methods for analyte detection and quantitation
JP2010539982A (ja) 2007-10-01 2010-12-24 アプライド バイオシステムズ， エルエルシー チェイスライゲーション配列決定法
CN101889074A (zh) 2007-10-04 2010-11-17 哈尔西恩莫尔丘勒公司 采用电子显微镜对核酸聚合物测序
US20090139311A1 (en) 2007-10-05 2009-06-04 Applied Biosystems Inc. Biological Analysis Systems, Devices, and Methods
EP2051051B1 (en) 2007-10-16 2020-06-03 Cambridge Research & Instrumentation, Inc. Spectral imaging system with dynamic optical correction
WO2009065093A2 (en) 2007-11-17 2009-05-22 University Of Medicine And Dentistry Of New Jersey Use of stem cells for wound healing
CN101586150B (zh) 2008-05-23 2016-09-28 陕西佰美基因股份有限公司 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途
US8199999B2 (en) 2008-06-17 2012-06-12 Cambridge Research & Instrumentation, Inc. Image classifier training
WO2010011961A2 (en) 2008-07-25 2010-01-28 University Of Georgia Research Foundation, Inc. Prokaryotic rnai-like system and methods of use
WO2010017264A2 (en) 2008-08-05 2010-02-11 Cornell University Photo-crosslinked nucleic acid hydrogels
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
US9896720B2 (en) 2008-09-10 2018-02-20 Rutgers, The State University Of New Jersey Imaging individual mRNA molecules using multiple singly labeled probes
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
US20100087325A1 (en) 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
WO2010046796A1 (en) * 2008-10-23 2010-04-29 Koninklijke Philips Electronics, N.V. Molecular imaging
US9404098B2 (en) 2008-11-06 2016-08-02 University Of Georgia Research Foundation, Inc. Method for cleaving a target RNA using a Cas6 polypeptide
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP3150724A1 (en) 2008-12-19 2017-04-05 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP4528345B2 (ja) 2009-01-28 2010-08-18 シャープ株式会社 動画再生装置、動画再生方法、動画再生方法をコンピュータで実現するためのプログラム及びそのプログラムを記録した記録媒体
JP5277082B2 (ja) 2009-06-15 2013-08-28 株式会社日立ハイテクノロジーズ 蛍光分析方法
FR2948475A1 (fr) 2009-07-24 2011-01-28 Bionext Procede de caracterisation d'objets tridimensionnels
US8431151B2 (en) * 2009-08-06 2013-04-30 Syracuse University Antimicrobial nanostructured hydrogel web containing silver
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
CA2778249C (en) 2009-11-03 2018-12-04 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection sequencing (qnps)
EP2531220B1 (en) 2009-12-18 2018-05-02 President and Fellows of Harvard College Active scaffolds for on-demand drug and cell delivery
US8632976B2 (en) 2010-01-19 2014-01-21 Agdia Amplification of TRP1 for specific detection of Phytophthora ramorum
EP2516681B1 (en) 2010-02-11 2017-10-18 Nanostring Technologies, Inc Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation
WO2011100617A2 (en) 2010-02-12 2011-08-18 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
WO2011112634A2 (en) 2010-03-08 2011-09-15 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
US10087431B2 (en) 2010-03-10 2018-10-02 The Regents Of The University Of California Methods of generating nucleic acid fragments
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
US8462981B2 (en) 2010-04-07 2013-06-11 Cambridge Research & Instrumentation, Inc. Spectral unmixing for visualization of samples
MX2012013037A (es) 2010-05-10 2013-07-29 Univ California Composiciones de endorribonucleasa y metodos de uso de las mismas.
US20130059741A1 (en) 2010-05-13 2013-03-07 Illumina, Inc. Binding assays for markers
WO2012005595A2 (en) 2010-07-09 2012-01-12 Wouter Leonard De Laat V3-d genomic region of interest sequencing strategies
EP2601513B1 (en) 2010-08-05 2014-05-14 Cambridge Research & Instrumentation, Inc. Enhancing visual assessment of samples
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods
US8658361B2 (en) 2010-10-21 2014-02-25 Advanced Cell Diagnostics, Inc. Ultra sensitive method for in situ detection of nucleic acids
WO2012056440A1 (en) 2010-10-28 2012-05-03 Nanodoc Ltd. COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA
WO2012057689A1 (en) 2010-10-29 2012-05-03 Ge Healthcare Bio-Sciences Ab Proximity ligation technology for western blot applications
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
US8859201B2 (en) 2010-11-16 2014-10-14 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
CN103649331A (zh) 2011-02-15 2014-03-19 莱卡生物系统纽卡斯尔有限责任公司 用于mrna的定位的原位检测的方法
JP5687514B2 (ja) 2011-02-17 2015-03-18 株式会社日立ハイテクノロジーズ 核酸配列解析装置
DK2500436T3 (en) 2011-03-17 2016-08-29 Pasteur Institut A method, probe and kit for in situ hybridization of DNA and use thereof
US20120252686A1 (en) 2011-03-31 2012-10-04 Good Start Genetics Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8946389B2 (en) * 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
WO2012150035A1 (de) 2011-05-04 2012-11-08 Genovoxx Gmbh Nukleosid-triphosphat-konjugate und methoden zu deren anwendung
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
EP2714938B1 (en) 2011-05-27 2017-11-15 President and Fellows of Harvard College Methods of amplifying whole genome of a single cell
WO2012164565A1 (en) 2011-06-01 2012-12-06 Yeda Research And Development Co. Ltd. Compositions and methods for downregulating prokaryotic genes
PL222511B1 (pl) 2011-06-08 2016-08-31 Międzynarodowy Inst Biologii Molekularnej I Komórkowej W Warszawie Endorybonukleazy dsRNA
EP3604555B1 (en) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
EP4249605B1 (en) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Methods for analyte detection
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
US20150166969A1 (en) 2012-02-24 2015-06-18 Fred Hutchinson Cancer Research Center Compositions and methods for the treatment of hemoglobinopathies
CA2865575C (en) * 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
WO2013130824A1 (en) 2012-02-29 2013-09-06 Sangamo Biosciences, Inc. Methods and compositions for treating huntington's disease
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
SG196730A1 (en) 2012-07-16 2014-02-13 Agency Science Tech & Res Methods for reading data from a storage medium using a reader and storage devices
ES2805794T3 (es) 2012-07-20 2021-02-15 Lsi Medience Corp Procedimiento de fotoacoplamiento que utiliza una sonda que contiene ácidos nucleicos fotosensibles
WO2014022702A2 (en) 2012-08-03 2014-02-06 The Regents Of The University Of California Methods and compositions for controlling gene expression by rna processing
KR102148747B1 (ko) 2012-08-09 2020-08-27 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 쥬니어 유니버시티 현미경적 분석을 위한 생물학적 표본을 준비하기 위한 방법과 조성물
WO2014028538A2 (en) 2012-08-13 2014-02-20 William Marsh Rice University Multiplexed in situ molecular analyses and programmable molecular probes for regulated signal amplification
AU2013302966B2 (en) 2012-08-15 2017-06-08 AURAD GmbH Systems and methods for imaging tissue
US10032064B2 (en) 2012-08-21 2018-07-24 Cambridge Research & Instrumentation, Inc. Visualization and measurement of cell compartments
CN102908119A (zh) 2012-09-26 2013-02-06 温州医学院眼视光研究院 一种共焦扫描成像系统及其像差控制方法
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
AU2013335451C1 (en) 2012-10-23 2024-07-04 Toolgen Incorporated Composition for cleaving a target DNA comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof
EP2920324B1 (en) 2012-11-14 2017-12-27 Olink Bioscience AB Localised rca-based amplification method
PL3360964T3 (pl) 2012-12-06 2020-03-31 Sigma-Aldrich Co. Llc Modyfikacja i regulacja genomu oparta na crispr
IL239344B2 (en) 2012-12-12 2024-06-01 Broad Inst Inc Systems engineering, methods and optimal guiding components for sequence manipulation
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
EP2840140B2 (en) 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
EP3031921B1 (en) 2012-12-12 2025-03-12 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
WO2014093655A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
DK2931891T3 (da) 2012-12-17 2019-08-19 Harvard College Rna-styret modificering af menneskelige genomer
WO2014110027A1 (en) 2013-01-10 2014-07-17 Caliper Life Sciences, Inc. Multispectral imaging system and methods
WO2014110025A1 (en) 2013-01-10 2014-07-17 Caliper Life Sciences, Inc. Whole slide multispectral imaging systems and methods
CA2898184A1 (en) 2013-01-16 2014-07-24 Emory University Cas9-nucleic acid complexes and uses related thereto
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
EP2971184B1 (en) 2013-03-12 2019-04-17 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
RU2662932C2 (ru) 2013-03-14 2018-07-31 Карибо Биосайенсиз, Инк. Композиции и способы с участием нуклеиновых кислот, нацеленных на нуклеиновые кислоты
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US20140273230A1 (en) 2013-03-15 2014-09-18 Sigma-Aldrich Co., Llc Crispr-based genome modification and regulation
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
CN112301024A (zh) 2013-03-15 2021-02-02 通用医疗公司 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性
US9330295B2 (en) 2013-03-15 2016-05-03 Brown University Spatial sequencing/gene expression camera
US10510435B2 (en) * 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
AU2014273082B2 (en) 2013-05-29 2018-11-08 Cellectis A method for producing precise DNA cleavage using Cas9 nickase activity
US9267135B2 (en) 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
SG10201913068PA (en) 2013-06-04 2020-02-27 Harvard College Rna-guided transcriptional regulation
CN105492611A (zh) 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US10794802B2 (en) 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
EP3047271B1 (en) 2013-09-20 2022-05-11 California Institute Of Technology Methods for phenotyping of intact whole tissues
US10539772B2 (en) 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
GB201401885D0 (en) * 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
EP3108218A4 (en) 2014-02-21 2017-11-15 Massachusetts Institute Of Technology Expansion microscopy
CN105981083B (zh) 2014-02-24 2019-01-08 索尼公司 智能可穿戴设备和用于优化输出的方法
CN106164736A (zh) 2014-05-30 2016-11-23 斯坦福大学托管董事会 用于对大的完整组织样品进行成像的方法及装置
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
US10126242B2 (en) 2014-07-09 2018-11-13 Caliper Life Sciences, Inc. Pure spectrum extraction from biological samples in fluorescence multispectral imaging
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP3169810B1 (en) 2014-07-17 2024-09-04 California Institute of Technology Multiplex analysis of molecules in single cells by image correlation
EP4328322A3 (en) 2014-07-30 2024-05-22 President and Fellows of Harvard College Probe library construction
US11091810B2 (en) 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US20160108458A1 (en) 2014-10-06 2016-04-21 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed detection and quantification of nucleic acids in single-cells
WO2016081740A1 (en) 2014-11-21 2016-05-26 Nanostring Technologies, Inc. Enzyme- and amplification-free sequencing
KR102602507B1 (ko) 2014-11-24 2023-11-14 나노스트링 테크놀로지스, 인크. 유전자 정제 및 이미징을 위한 방법 및 장치
CA2969038C (en) 2014-12-03 2023-07-04 Ventana Medical Systems, Inc. Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
US9607375B2 (en) 2015-06-03 2017-03-28 Eileen B. Gallagher Biological data annotation and visualization
WO2017015097A1 (en) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
SG10202107053QA (en) 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
WO2017019481A1 (en) 2015-07-24 2017-02-02 The Johns Hopkins University Compositions and methods of rna analysis
EP3332034B1 (en) 2015-08-06 2024-09-25 Arc Bio, LLC Systems and methods for genomic analysis
EP3332258B1 (en) 2015-08-07 2020-01-01 Massachusetts Institute of Technology Protein retention expansion microscopy
US10364457B2 (en) 2015-08-07 2019-07-30 Massachusetts Institute Of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
US11214661B2 (en) 2015-09-17 2022-01-04 Massachusetts Institute Of Technology Three-dimensional nanofabrication by patterning of hydrogels
WO2017075275A1 (en) 2015-10-29 2017-05-04 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for imaging a biological sample
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
US20190155835A1 (en) 2015-11-03 2019-05-23 President And Fellows Of Harvard College Systems and Methods for Processing Spatially Related Sequence Data Received from a Sequencing Device
CN108700460B (zh) 2015-12-21 2020-11-03 威里利生命科学有限责任公司 成像系统和成像方法
WO2017139501A1 (en) 2016-02-10 2017-08-17 The Board Of Trustees Of The Leland Stanford Junior University Rna fixation and detection in clarity-based hydrogel tissue
US20210207131A1 (en) 2016-02-18 2021-07-08 President And Fellows Of Harvard College Multiplex Alteration of Cells Using a Pooled Nucleic Acid Library and Analysis Thereof
US11360025B2 (en) 2016-02-22 2022-06-14 Miltenyi Biotec B.V. & Co. KG Automated analysis tool for biological specimens
US20170253918A1 (en) 2016-03-01 2017-09-07 Expansion Technologies Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics
WO2017161251A1 (en) 2016-03-17 2017-09-21 President And Fellows Of Harvard College Methods for detecting and identifying genomic nucleic acids
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US20180052081A1 (en) 2016-05-11 2018-02-22 Expansion Technologies Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics
CN116200462B (zh) 2016-05-16 2025-10-03 布鲁克空间生物学公司 用于检测样品中目标核酸的方法
EP3252452A1 (en) 2016-05-25 2017-12-06 The Board of Trustees of the Leland Stanford Junior University Method for imaging and analysis of a biological specimen
IL290679B2 (en) * 2016-07-05 2023-10-01 California Inst Of Techn Hybridization chain reaction based on a minimal initiator
AU2017302300B2 (en) 2016-07-27 2023-08-17 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
US12421540B2 (en) 2016-08-01 2025-09-23 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
CA3034617A1 (en) 2016-08-30 2018-03-08 California Institute Of Technology Immunohistochemistry via hybridization chain reaction
EP3539036A4 (en) 2016-11-08 2020-06-17 President and Fellows of Harvard College MATRIX AND DELETE
EP4339956A3 (en) 2016-11-08 2024-06-19 President and Fellows of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
EP4556575A3 (en) 2016-11-21 2025-10-08 Bruker Spatial Biology, Inc. A method for sequencing nucleic acids
EP3601339A4 (en) * 2017-03-31 2020-11-04 Ultivue, Inc. DNA ANTIGEN EXCHANGE AND AMPLIFICATION
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
US11753676B2 (en) 2017-10-11 2023-09-12 Expansion Technologies Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy
WO2019103996A1 (en) 2017-11-21 2019-05-31 Expansion Technologies Expansion microscopy compatible and multiplexed in situ hybridization of formalin fixed paraffin embedded tissue sections for spatially resolved transcriptomics
EP3720972A1 (en) 2017-12-08 2020-10-14 California Institute of Technology Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridzation of probes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060234261A1 (en) * 2005-03-08 2006-10-19 Pierce Niles A Colorimetric readout of hybridization chain reaction
CN105392898A (zh) * 2013-04-30 2016-03-09 加州理工学院 通过顺序杂交编条形码的分子多重标记

Also Published As

Publication number Publication date
AU2023210577A1 (en) 2023-08-24
EP3449016B1 (en) 2025-07-23
EP4613756A2 (en) 2025-09-10
US20210381049A1 (en) 2021-12-09
EP4613756A3 (en) 2025-11-12
CN109415761B (zh) 2022-09-20
AU2017257624B2 (en) 2023-05-25
CA3022290A1 (en) 2017-11-02
JP2019518434A (ja) 2019-07-04
US20190218608A1 (en) 2019-07-18
US20240124932A1 (en) 2024-04-18
JP7259182B2 (ja) 2023-04-18
CN109415761A (zh) 2019-03-01
AU2017257624A1 (en) 2018-11-22
US11713485B2 (en) 2023-08-01
EP3449016A4 (en) 2019-10-02
EP3449016A1 (en) 2019-03-06
CA3210120A1 (en) 2017-11-02
US11718874B2 (en) 2023-08-08
WO2017189525A1 (en) 2017-11-02
CA3210120C (en) 2024-04-09

Similar Documents

Publication Publication Date Title
US11713485B2 (en) Hybridization chain reaction methods for in situ molecular detection
EP2647426A1 (en) Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
EP3058091A1 (en) Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
CN106435744B (zh) 使用y-接头和消失限制位点的文库构建
CN114729349A (zh) 条码化核酸用于检测和测序的方法
EP4448792A1 (en) Hybrid clustering
US20240158784A1 (en) Molecular barcodes and related methods and systems
JP2024509822A5 (https=)
EP2456892B1 (en) Method for sequencing a polynucleotide template
WO2024256580A1 (en) Concurrent sequencing with spatially separated rings
JP2002233385A (ja) 核酸の増幅方法及びそれに用いられるプライマー固定化担体
Mi et al. Multiplexed RNA imaging and in situ profiling in living cells
WO2026080906A1 (en) In-solution methods for determination of protein information by recoding amino acid polymers into dna polymers
CN121135811A (zh) 蛋白质标记方法及其应用
WO2024215735A1 (en) Multiplexed optical barcoding for spatial omics
WO2025062002A1 (en) Concurrent sequencing using nick translation

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20230602