JP6975507B2 - 二本鎖配列決定のための改善されたアダプター、方法、及び組成物 - Google Patents

二本鎖配列決定のための改善されたアダプター、方法、及び組成物 Download PDF

Info

Publication number
JP6975507B2
JP6975507B2 JP2018530698A JP2018530698A JP6975507B2 JP 6975507 B2 JP6975507 B2 JP 6975507B2 JP 2018530698 A JP2018530698 A JP 2018530698A JP 2018530698 A JP2018530698 A JP 2018530698A JP 6975507 B2 JP6975507 B2 JP 6975507B2
Authority
JP
Japan
Prior art keywords
nucleic acid
adapter
strand
double
stranded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018530698A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019509015A5 (enExample
JP2019509015A (ja
Inventor
ローブ,ローレンス・エー
シュミット,マイケル・ダブリュー
ソーク,ジェシー・ジェイ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Twinstrand Biosciences Inc
Original Assignee
Twinstrand Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Twinstrand Biosciences Inc filed Critical Twinstrand Biosciences Inc
Publication of JP2019509015A publication Critical patent/JP2019509015A/ja
Publication of JP2019509015A5 publication Critical patent/JP2019509015A5/ja
Priority to JP2021179334A priority Critical patent/JP7548579B2/ja
Application granted granted Critical
Publication of JP6975507B2 publication Critical patent/JP6975507B2/ja
Priority to JP2023184524A priority patent/JP2024010122A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2018530698A 2015-12-08 2016-12-08 二本鎖配列決定のための改善されたアダプター、方法、及び組成物 Active JP6975507B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021179334A JP7548579B2 (ja) 2015-12-08 2021-11-02 二本鎖配列決定のための改善されたアダプター、方法、及び組成物
JP2023184524A JP2024010122A (ja) 2015-12-08 2023-10-27 二本鎖配列決定のための改善されたアダプター、方法、及び組成物

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562264822P 2015-12-08 2015-12-08
US62/264,822 2015-12-08
US201662281917P 2016-01-22 2016-01-22
US62/281,917 2016-01-22
PCT/US2016/065605 WO2017100441A1 (en) 2015-12-08 2016-12-08 Improved adapters, methods, and compositions for duplex sequencing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2021179334A Division JP7548579B2 (ja) 2015-12-08 2021-11-02 二本鎖配列決定のための改善されたアダプター、方法、及び組成物

Publications (3)

Publication Number Publication Date
JP2019509015A JP2019509015A (ja) 2019-04-04
JP2019509015A5 JP2019509015A5 (enExample) 2020-01-23
JP6975507B2 true JP6975507B2 (ja) 2021-12-01

Family

ID=57750605

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2018530698A Active JP6975507B2 (ja) 2015-12-08 2016-12-08 二本鎖配列決定のための改善されたアダプター、方法、及び組成物
JP2021179334A Active JP7548579B2 (ja) 2015-12-08 2021-11-02 二本鎖配列決定のための改善されたアダプター、方法、及び組成物
JP2023184524A Pending JP2024010122A (ja) 2015-12-08 2023-10-27 二本鎖配列決定のための改善されたアダプター、方法、及び組成物

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2021179334A Active JP7548579B2 (ja) 2015-12-08 2021-11-02 二本鎖配列決定のための改善されたアダプター、方法、及び組成物
JP2023184524A Pending JP2024010122A (ja) 2015-12-08 2023-10-27 二本鎖配列決定のための改善されたアダプター、方法、及び組成物

Country Status (11)

Country Link
US (3) US11332784B2 (enExample)
EP (2) EP3387152B1 (enExample)
JP (3) JP6975507B2 (enExample)
CN (1) CN109072294A (enExample)
AU (1) AU2016366231B2 (enExample)
CA (1) CA3006792A1 (enExample)
ES (1) ES2911421T3 (enExample)
IL (1) IL259788B2 (enExample)
PL (1) PL3387152T3 (enExample)
PT (1) PT3387152T (enExample)
WO (1) WO2017100441A1 (enExample)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
SMT202100154T1 (it) 2012-02-17 2021-05-07 Hutchinson Fred Cancer Res Composizioni e metodi per identificare precisamente mutazioni
PL2828218T3 (pl) 2012-03-20 2021-01-11 University Of Washington Through Its Center For Commercialization Sposoby obniżania współczynnika błędów masywnie równoległej sekwencji dna z wykorzystaniem duplex consensus sequencing
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
EP4610368A3 (en) 2013-08-05 2025-11-05 Twist Bioscience Corporation De novo synthesized gene libraries
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
WO2016078095A1 (zh) 2014-11-21 2016-05-26 深圳华大基因科技有限公司 鼓泡状接头元件和使用其构建测序文库的方法
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
HK1258869A1 (zh) 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
EP3387152B1 (en) 2015-12-08 2022-01-26 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
CN117174167A (zh) 2015-12-17 2023-12-05 夸登特健康公司 通过分析无细胞dna确定肿瘤基因拷贝数的方法
EP4488686A3 (en) 2016-04-14 2025-04-30 Guardant Health, Inc. Methods for early detection of cancer
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
EP3485033B1 (en) 2016-07-12 2022-09-28 Qiagen Sciences, LLC Single end duplex dna sequencing
ES2875318T3 (es) * 2016-07-18 2021-11-10 Hoffmann La Roche Procedimiento para generar colecciones de ADN circular monocatenario para secuenciación de molécula única
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
AU2017315294B2 (en) 2016-08-22 2023-12-21 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP4664304A2 (en) 2016-09-21 2025-12-17 Atlas Data Storage, Inc. Nucleic acid based data storage
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN117004721A (zh) 2016-12-28 2023-11-07 奎斯特诊断投资有限责任公司 用于检测循环肿瘤dna的组合物和方法
EP3571616B1 (en) 2017-01-18 2021-05-19 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11479807B2 (en) 2017-03-23 2022-10-25 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
AU2018266377B2 (en) 2017-05-08 2024-06-20 Illumina, Inc. Universal short adapters for indexing of polynucleotide samples
KR102784398B1 (ko) 2017-06-12 2025-03-19 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
US11447818B2 (en) * 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES
ES2966831T3 (es) 2017-11-06 2024-04-24 Illumina Inc Técnicas de indexación de ácidos nucleicos
US11739367B2 (en) 2017-11-08 2023-08-29 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
EP3735459A4 (en) 2018-01-04 2021-10-06 Twist Bioscience Corporation DNA-BASED DIGITAL INFORMATION STORAGE
CN108300716B (zh) * 2018-01-05 2020-06-30 武汉康测科技有限公司 接头元件、其应用和基于不对称多重pcr进行靶向测序文库构建的方法
AU2019233918B2 (en) 2018-03-15 2025-09-11 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations
GB201804641D0 (en) 2018-03-22 2018-05-09 Inivata Ltd Methods of sequencing nucleic acids and error correction of sequence reads
EP3768853A4 (en) 2018-03-23 2021-04-28 Board Of Regents The University Of Texas System EFFICIENT SEQUENCING OF DSDNA WITH EXTREMELY LOW ERROR RATE
AU2019269635A1 (en) * 2018-05-16 2020-11-26 Twinstrand Biosciences, Inc. Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications
IL278771B2 (en) 2018-05-18 2025-09-01 Twist Bioscience Corp Polynucleotides, reagents and methods for nucleic acid hybridization
CN112673099A (zh) * 2018-07-12 2021-04-16 特温斯特兰德生物科学有限公司 用于表征基因组编辑、克隆扩增的方法和试剂及相关应用
EP3844300A1 (en) * 2018-08-28 2021-07-07 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
WO2020058389A1 (en) * 2018-09-21 2020-03-26 F. Hoffmann-La Roche Ag System and method for modular and combinatorial nucleic acid sample preparation for sequencing
CA3115819A1 (en) 2018-10-16 2020-04-23 Twinstrand Biosciences, Inc. Methods and reagents for efficient genotyping of large numbers of samples via pooling
US11358137B2 (en) 2018-12-26 2022-06-14 Industrial Technology Research Institute Tubular structure for producing droplets and method for producing droplets
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
JP2022521766A (ja) * 2019-02-25 2022-04-12 ツイスト バイオサイエンス コーポレーション 次世代シーケンシングのための組成物および方法
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
EP3930753A4 (en) 2019-02-26 2023-03-29 Twist Bioscience Corporation VARIANT NUCLEIC ACID LIBRARIES FOR GLP1 RECEPTOR
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
AU2020321991A1 (en) * 2019-08-01 2022-03-03 Twinstrand Biosciences, Inc. Methods and reagents for nucleic acid sequencing and associated applications
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
WO2021061829A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
ES3041276T3 (en) 2019-11-06 2025-11-11 Univ Leland Stanford Junior Methods for analyzing nucleic acid molecules
US11783912B2 (en) 2021-05-05 2023-10-10 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for analyzing nucleic acid molecules
EP4555084A2 (en) * 2022-07-12 2025-05-21 University of Washington Systems and methods for variant detection in cells
CN119907855A (zh) * 2022-07-25 2025-04-29 深圳华大智造科技股份有限公司 用于长dna分子测序的溶液中位置协同条形码化的方法

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5308751A (en) 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
ATE364718T1 (de) * 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
WO2000024939A1 (en) 1998-10-27 2000-05-04 Affymetrix, Inc. Complexity management and analysis of genomic dna
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
CA2741049C (en) 2001-10-15 2019-02-05 Bioarray Solutions, Ltd. Multiplexed analysis of polymorphic loci by probe elongation-mediated detection
US7406385B2 (en) 2001-10-25 2008-07-29 Applera Corporation System and method for consensus-calling with per-base quality values for sample assemblies
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
US7452699B2 (en) 2003-01-15 2008-11-18 Dana-Farber Cancer Institute, Inc. Amplification of DNA in a hairpin structure, and applications
WO2004081183A2 (en) 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
CA2552858A1 (en) 2004-01-23 2005-08-04 Lingvitae As Improving polynucleotide ligation reactions
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US8796441B2 (en) 2005-04-13 2014-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Human sweet and umami taste receptor variants
ATE491045T1 (de) 2005-06-23 2010-12-15 Keygene Nv Strategien mit hohem durchsatz zur identifizierung und zum nachweis von polymorphismen
EP1929039B2 (en) 2005-09-29 2013-11-20 Keygene N.V. High throughput screening of mutagenized populations
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7537897B2 (en) 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
US20070172839A1 (en) 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
PL2963127T3 (pl) 2006-04-04 2018-01-31 Keygene Nv Wysokoprzepustowa detekcja markerów molekularnych na podstawie fragmentów restrykcyjnych
WO2008093098A2 (en) * 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2201143B2 (en) 2007-09-21 2016-08-24 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
EP3170904B1 (en) 2008-03-28 2017-08-16 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110301042A1 (en) 2008-11-11 2011-12-08 Helicos Biosciences Corporation Methods of sample encoding for multiplex analysis of samples by single molecule sequencing
CA2750879C (en) 2009-01-30 2018-05-22 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US20100331204A1 (en) 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
US20120165202A1 (en) 2009-04-30 2012-06-28 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
FI3425062T3 (fi) 2010-06-09 2023-09-01 Keygene Nv Kombinatorisia sekvenssiviivakoodeja suuren suorituskyvyn seulontaa varten
US20120238738A1 (en) 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US20120244525A1 (en) 2010-07-19 2012-09-27 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US8481292B2 (en) 2010-09-21 2013-07-09 Population Genetics Technologies Litd. Increasing confidence of allele calls with molecular counting
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
US9074251B2 (en) * 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
WO2012083225A2 (en) 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
WO2013009175A1 (en) 2011-07-08 2013-01-17 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
EP2753712B1 (en) 2011-09-06 2017-03-22 Gen-Probe Incorporated Closed nucleic acid structures
US9249460B2 (en) 2011-09-09 2016-02-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
JP5418579B2 (ja) 2011-12-06 2014-02-19 株式会社デンソー 開閉眼検出装置
CA2862552A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20160153039A1 (en) 2012-01-26 2016-06-02 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US9528107B2 (en) * 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
SMT202100154T1 (it) 2012-02-17 2021-05-07 Hutchinson Fred Cancer Res Composizioni e metodi per identificare precisamente mutazioni
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
EP3287531B1 (en) 2012-02-28 2019-06-19 Agilent Technologies, Inc. Method for attaching a counter sequence to a nucleic acid sample
NO2694769T3 (enExample) * 2012-03-06 2018-03-03
CA2867293C (en) 2012-03-13 2020-09-01 Abhijit Ajit PATEL Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
PL2828218T3 (pl) 2012-03-20 2021-01-11 University Of Washington Through Its Center For Commercialization Sposoby obniżania współczynnika błędów masywnie równoległej sekwencji dna z wykorzystaniem duplex consensus sequencing
CA2873176C (en) 2012-05-10 2024-02-27 The General Hospital Corporation Methods for determining a nucleotide sequence
EP2855707B1 (en) 2012-05-31 2017-07-12 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
CN102877136B (zh) * 2012-09-24 2014-03-12 上海交通大学 基于基因组简化与二代测序dna文库构建方法及试剂盒
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
US9290800B2 (en) * 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
US10087481B2 (en) 2013-03-19 2018-10-02 New England Biolabs, Inc. Enrichment of target sequences
US9873907B2 (en) 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
WO2015048084A1 (en) * 2013-09-24 2015-04-02 Somalogic, Inc. Multiaptamer target detection
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
EP3099796B1 (en) 2014-01-31 2018-08-29 Swift Biosciences, Inc. Improved methods for processing dna substrates
ES2925014T3 (es) 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
US11149305B2 (en) 2015-01-23 2021-10-19 Washington University Detection of rare sequence variants, methods and compositions therefor
US10686637B2 (en) 2015-02-20 2020-06-16 Nec Corporation Receiving apparatus and receiving method
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10465241B2 (en) 2015-06-15 2019-11-05 The Board Of Trustees Of The Leleand Stanford Junior University High resolution STR analysis using next generation sequencing
GB201515557D0 (en) 2015-09-02 2015-10-14 14M Genomics Ltd Method of sequencing
CN108350491A (zh) 2015-11-18 2018-07-31 加利福尼亚太平洋生物科学股份有限公司 将核酸加载到基材上
EP3387152B1 (en) 2015-12-08 2022-01-26 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
WO2017127742A1 (en) 2016-01-22 2017-07-27 Grail, Inc. Variant based disease diagnostics and tracking
CN108603229A (zh) 2016-01-22 2018-09-28 格瑞尔公司 用于高保真测序的方法和系统
EP3485033B1 (en) 2016-07-12 2022-09-28 Qiagen Sciences, LLC Single end duplex dna sequencing
CN107664703B (zh) 2016-07-29 2022-07-08 希森美康株式会社 标本运送装置、标本图像拍摄系统及标本分析系统
WO2018031588A1 (en) 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
US10487358B2 (en) 2016-09-23 2019-11-26 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
US10982351B2 (en) 2016-12-23 2021-04-20 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
US11479807B2 (en) 2017-03-23 2022-10-25 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
US11739367B2 (en) 2017-11-08 2023-08-29 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
WO2019160998A1 (en) 2018-02-13 2019-08-22 Twinstrand Biosciences, Inc. Methods and reagents for detecting and assessing genotoxicity
AU2019233918B2 (en) 2018-03-15 2025-09-11 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations
EP3768853A4 (en) 2018-03-23 2021-04-28 Board Of Regents The University Of Texas System EFFICIENT SEQUENCING OF DSDNA WITH EXTREMELY LOW ERROR RATE
AU2019269635A1 (en) 2018-05-16 2020-11-26 Twinstrand Biosciences, Inc. Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications
CN112673099A (zh) 2018-07-12 2021-04-16 特温斯特兰德生物科学有限公司 用于表征基因组编辑、克隆扩增的方法和试剂及相关应用
KR102662200B1 (ko) 2019-07-15 2024-04-29 엘지디스플레이 주식회사 유기발광표시장치

Also Published As

Publication number Publication date
EP4043584A1 (en) 2022-08-17
PT3387152T (pt) 2022-04-19
CN109072294A (zh) 2018-12-21
PL3387152T3 (pl) 2022-05-09
IL259788B2 (en) 2023-05-01
US20170211140A1 (en) 2017-07-27
IL259788B1 (en) 2023-01-01
IL259788A (en) 2018-07-31
US11332784B2 (en) 2022-05-17
ES2911421T3 (es) 2022-05-19
JP2024010122A (ja) 2024-01-23
EP3387152B1 (en) 2022-01-26
JP7548579B2 (ja) 2024-09-10
JP2019509015A (ja) 2019-04-04
AU2016366231A1 (en) 2018-06-14
US20250313888A1 (en) 2025-10-09
AU2016366231B2 (en) 2022-12-15
CA3006792A1 (en) 2017-06-15
WO2017100441A1 (en) 2017-06-15
JP2022017453A (ja) 2022-01-25
US20220267841A1 (en) 2022-08-25
EP3387152A1 (en) 2018-10-17

Similar Documents

Publication Publication Date Title
JP6975507B2 (ja) 二本鎖配列決定のための改善されたアダプター、方法、及び組成物
US20240141426A1 (en) Compositions and methods for identification of a duplicate sequencing read
CN110248675B (zh) 利用竞争性链置换构建下一代测序(ngs)文库
EP3408406B1 (en) A novel y-shaped adaptor for nucleic acid sequencing and method of use
US20180223350A1 (en) Duplex adapters and duplex sequencing
CN115516108A (zh) 评估核酸的方法和材料
JP6876785B2 (ja) 単分子配列決定のための一本鎖環状dnaライブラリーを生成するための方法
CN104093854A (zh) 表征组合物中的rna的方法和试剂盒
JP2025522572A (ja) 核酸配列決定のための方法及び組成物
US20250163492A1 (en) Method for generating population of labeled nucleic acid molecules and kit for the method
US20240052339A1 (en) Rna probe for mutation profiling and use thereof
US20250297301A1 (en) Single-stranded end preserving adaptors
HK1227923A1 (en) Compositions and methods for identification of a duplicate sequencing read

Legal Events

Date Code Title Description
A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20181221

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20181221

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191206

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20191206

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210112

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210409

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210609

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210709

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20211005

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20211102

R150 Certificate of patent or registration of utility model

Ref document number: 6975507

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250