AU2016366231B2 - Improved adapters, methods, and compositions for duplex sequencing - Google Patents

Improved adapters, methods, and compositions for duplex sequencing Download PDF

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AU2016366231B2
AU2016366231B2 AU2016366231A AU2016366231A AU2016366231B2 AU 2016366231 B2 AU2016366231 B2 AU 2016366231B2 AU 2016366231 A AU2016366231 A AU 2016366231A AU 2016366231 A AU2016366231 A AU 2016366231A AU 2016366231 B2 AU2016366231 B2 AU 2016366231B2
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nucleic acid
adapter
strand
sequence
target nucleic
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AU2016366231A1 (en
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Lawrence A. Loeb
Jesse J. SALK
Michael W. Schmitt
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Twinstrand Biosciences Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2016366231A 2015-12-08 2016-12-08 Improved adapters, methods, and compositions for duplex sequencing Active AU2016366231B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562264822P 2015-12-08 2015-12-08
US62/264,822 2015-12-08
US201662281917P 2016-01-22 2016-01-22
US62/281,917 2016-01-22
PCT/US2016/065605 WO2017100441A1 (en) 2015-12-08 2016-12-08 Improved adapters, methods, and compositions for duplex sequencing

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AU2016366231A1 AU2016366231A1 (en) 2018-06-14
AU2016366231B2 true AU2016366231B2 (en) 2022-12-15

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US (3) US11332784B2 (enExample)
EP (2) EP3387152B1 (enExample)
JP (3) JP6975507B2 (enExample)
CN (1) CN109072294A (enExample)
AU (1) AU2016366231B2 (enExample)
CA (1) CA3006792A1 (enExample)
ES (1) ES2911421T3 (enExample)
IL (1) IL259788B2 (enExample)
PL (1) PL3387152T3 (enExample)
PT (1) PT3387152T (enExample)
WO (1) WO2017100441A1 (enExample)

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